• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.1
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• pp.57-62
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• 2002

Cationic liposomes complexed with DNA have been extensively utilized for the delivery of reporter or therapeutic genes both in culture and in vivo. We investigated and determined the optimum conditions of a cationic liposome, composed of dimethyldioctadecy-lammonium bromide (DDAB) and dioleoyl phosphati-dylethanolamine UOPE), mediated a reporter plasmid expressing luciferase into insect cell lines (Sf-21 and Bm-N) and silkworm larvae. Together the data demonstrated that Bombyx mori nuclear polyhedrosis virus (BmNPV) genomic DNA (128 kb) was successfully transfected into Bm-5 cells using this liposome. These results suggest that DDAB/DOPE liposome will be useful as delivery agents for gene transfer to insect cells both in vitro and in vivo.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.2
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• pp.119-122
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• 2001

Whether Bombyx mori nuclear polyhedrosis virus (BmNPV) can be transmitted to offspring, has been a noticeable question for a long time. When fifth instar larvae of the silkworm were orally inoculated with BmNPV dot hybridization and PCR amplification analysis demonstrated that BmNPV was not detected in the eggs laid by BmNPV productively infected female moths. The results indicated that BmNPV could not be transovarially transmitted.

• Korean Journal of Clinical Laboratory Science
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• v.53 no.1
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• pp.68-80
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• 2021

An uncertified facility is a facility not certified, as defined in the Biological Agents and Toxins Act (BATA) in Singapore, but has met the requirements of the Ministry of Health to possess First and Fifth Schedule biological agents and toxins. This type of facility is also known as a Biosafety Level 2 Plus (BSL 2+) facility. Registration as an uncertified facility or a BSL 2+ facility requires a certain process and procedure to be sought with the Biosafety Branch of the ministry. This review, shares first-hand knowledge on the journey to achieving registration of the authors' facility. The procedure involved considerable preparation, setting up facility requirements, biosafety precautions, procedures and practices, and training and competence of laboratory users. The ministry conducted a thorough onsite facility audit to ensure that the facility requirements and biosafety procedures and practices were in place. It then issued an approval letter of possession for the first-time use of biological agents and registered the laboratory as an uncertified facility. The expectation is that the comprehensive information shared may be of great benefit to other facilities with similar interests.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.12
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• pp.1939-1945
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• 2018

Objective: This study was conducted with the objectives to examine the impacts of inorganic selenium (Se) and different types and levels of organic selenium on the serum and tissues Se status and antioxidant capacity in broiler breeders. Methods: Five hundred and forty 48-wk-old Lingnan Yellow broiler breeders were randomly assigned to 6 dietary treatments, provided same basal diet (0.04 mg/kg of Se) with 0.15 mg/kg, or 0.30 mg/kg of Se from sodium selenite (SS) or from selenium-enriched yeast (SY) or from selenomethionine (SM). The broiler breeders were slaughtered after an 8-wk experiment. Results: The results showed that SM was better than SY and SS, 0.30 mg/kg level was better than 0.15 mg/kg level in Se deposition (p<0.05) in serum, liver, kidney, pancreas and muscle; in antioxidant status, organic selenium had better effects than SS in broiler breeders (p<0.05), but SM and SY had a similar result, and 0.15 mg/kg level was better than 0.30 mg/kg (p<0.05). Conclusion: The results demonstrated the evident advantage of supplementation of broiler breeders with 0.15 mg/kg SM, which improved tissue Se concentrations and antioxidant status, and can be considered as the best selenium source.

• Journal of Distribution Science
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• v.18 no.12
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• pp.67-77
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• 2020

Purpose: Based on the author's adapted invariant choice, this study is to present the methodology and the calculation of the integral index of the digital component of the quality of life. By analyzing the digital indexes, the study is also to discuss distribution of ICT and the digital quality of life of the population of Kazakhstan and its regions. Research design, data, methodology: In this research, the method of calculation of integral assessment of the indicator was used, which indicates index constructs. The study analyzed objective secondary data for the period 2017-2019, which was the database from official websites of the Committee on Statistics of the Republic of Kazakhstan. Results: The study produced an integral code for assessing digital components of living standards of the population, consisting of five groups sub-indexes. Conclusions: Based on the provided analyses, we can confirm the existence of a significant difference of all the indicators of digital living standards of the population between the two leading cities: Almaty city and Nur-Sultan city. Furthermore we can deduce the differences of the examined indexes for other regions of Kazakhstan. Despite the rapid adoption of digital technologies, Kazakhstan still has significant digital gaps among cities indicating regional differences in the speed of implementation and distribution of digital technologies.

• The Journal of Asian Finance, Economics and Business
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• v.6 no.1
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• pp.321-328
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• 2019

The purpose of the study is to analyze the main approaches to assessing human capital and develop proposals for its most objective assessment of it at the different levels of manifestation - individual, microeconomic and macroeconomic. The article considers the basic approaches to the evaluation of human capital, used in practice: retrospective (costly) and prospective (income based). Cost based methods involve measuring the value of human capital based on the total costs associated with its formation. The proposed additional evaluation criteria, in particular, the use of the expert approach and the developed indicators of a qualitative assessment of the human capital of the enterprise will allow development that is more efficient and use of available human resources. Human capital is becoming a major factor in the formation and development of an innovative economy and knowledge economy. Accordingly, the proposed additions to the assessment of human capital at the country level are aimed at assessing it from a new angle, taking into account the current global trends in the formation of an innovative economy and digitalization. They meet qualitatively new requirements for human capital as the main productive factor in the creation of new highly efficient technologies that promote the active development of the social sphere, science, education, health, etc.

• Journal of Distribution Science
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• v.19 no.8
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• pp.13-24
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• 2021

Purpose: This research is aimed to explore factors affecting on Kazakhstan's pharmaceutical distribution industry, selection of various factors and assessment of the level of their influence. Based on the literature review it was defined that there is a great variety of scientific works relating to pharmaceutical distribution industry competitiveness and management improvement. Research design, data and methodology: There is very little research, which to determine the issues of pharmaceutical industry distribution in developing countries, in particular EAEU countries. The algorithm was chosen for research provision: statistical and comparative analysis, correlation, and regression analysis. The data of 1993-2020 obtained from the World Bank, Bureau of National Statistics, National Bank of Kazakhstan, which is expressed by 19 factors as macroeconomic indicators. Results: The chosen variables were selected non-randomly, these economic indicators had the most reliable, unique, and utmost for the whole research period complete information. Conclusions: There could be made adequate conclusions of the research, there is a strong positive relationship for six factors: population, GDP per capita, average annual US dollar exchange rate, the minimum pension, average assigned monthly pension, minimum wage. Pension and wage are the most significant factors affecting on the pharmaceutical distribution industry in Kazakhstan.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.8
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• pp.1102-1106
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• 2009

The Tibetan Mastiff is one of the most archaic, ferocious and the largest dogs in the world. The Kunming dog is the chief working-dog breed in China. In this study, ten microsatellite loci were used to assess the genetic diversity and evolutionary relationships in six dog breeds, including Tibetan Mastiff, Kunming dog, Belgian Malinois, Labrador Retriever, English Springer Spaniel, and German Shepherd. The highest genetic diversity was exhibited by the Tibetan Mastiff, indicating useful protection and little inbreeding in the modern Tibetan Mastiff. Higher genetic diversity was observed in European breeds, supporting the hypotheses that breeders outcross their pure breed dogs occasionally to avoid deleterious effects in Europe. Evolutionary relationships showed that English Springer Spaniel and Labrador Retriever were clustered together, then with the Tibetan Mastiff, consistent with previous cluster results. German Shepherd and Kunming dog were grouped together, coinciding with the breeding history of Kunming dog. It is the first time that Tibetan Mastiff and Kunming dog have been analyzed with microsatellites.

• Journal of the Optical Society of Korea
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• v.17 no.1
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• pp.5-9
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• 2013

We report on high-power continuous-wave operations of an Er:YAG ceramic laser in-band pumped by a cladding-pumped Er,Yb fiber laser at 1532 nm. With an output coupler of 10% transmission, the ceramic laser yielded 16.7 W of continuous-wave output at 1645 nm for 28.8 W of incident pump power, corresponding to a slope efficiency of 61.0% with respect to the incident pump power. The lasing wavelength switched to 1617 nm when output couplers of > 20% transmission were used. Up to 16.2 W of 1617 nm output was generated for 33.0 W of incident pump power, corresponding to a slope efficiency of 51.8%. Graphene Q-switched operation of Er:YAG cermic laser at 1645 nm was also demonstrated with stable pulses of 30-74 kHz repetition rates and 1.5-6.4 ${\mu}s$ pulse widths.

• Journal of Distribution Science
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• v.21 no.4
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• pp.47-56
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• 2023

Purpose: the research aims to analyze the impact of the distribution of knowledge through online learning on the intellectual potential of PhD students and produce recommendations for policy to improve intellectual capacity. During the literature review, it was determined that a large number of studies examined the impact of online learning on the quality of education at different levels. Research design, data and methodology: the research methodology is based on subjective assessment and studying the students' opinions. The basis of the study was a comprehensive analysis of primary data obtained through a sociological survey of PhD students. 324 respondents from humanitarian, medical and natural faculties participated in the survey. Results: the study revealed that online learning helps increase students' intellectual potential. PhD students had a positive attitude towards the transition from traditional education to online learning. It should be noted that, according to the results, the most popular gadgets were laptops and smartphones, which were characterized by high mobility and ease of use. Based on the obtained results, recommendations were developed for the formation of online learning with a focus on increasing students' intellectual potential. Conclusions: based on the results of the assessment of educational and innovative potential, policy recommendations and further research in this area were proposed.