• Applied Microscopy
• /
• v.29 no.1
• /
• pp.1-10
• /
• 1999

The aim of the present study was to investigate with transmission electron misroscope the comparative morphology of epididymal spermatozoa in two species of the Korean insectivorous bats belonging to 'prolonged sperm storage' type (Rhinolophus ferrumequinum korai) and 'delayed implantation' type (Miniopterus schreibersi fuliginosus). Sperm head of the R. ferrumequinum korai was bullet shaped and that of M. schreibersi fuliginosus was spatula shaped. The nuclei of the sperm head of the R. ferrumequinum korai and M. schreibersi fuliginosus occupied two-third and a half of it, respectively. The segmented columns of R. ferrumequinum korai were about 12 to 14 in number, and those of the M. schreibersi fuligincsus were about 10 to 12. Particularly, a pile of the satellite fibers in middle piece of R. ferrumequinum korai remained the inner aspect of the outer dense fibers, but those of the M. schreibersi fuliginosus were not.

• Applied Microscopy
• /
• v.39 no.3
• /
• pp.267-276
• /
• 2009

A SEM study on morphology of lingual papillae of Korean long-fingered bat (Miniopterus schreibersi fuliginosus) and Savi's Pipistrelle (Pipistrellus savii) was conducted. Three kinds of lingual papillae were observed: filiform, fungiform, circumvallate papillae. Filiform papillae were divided into two types; the type 1 had a group of needle-like projections, and was distributed throughout the front half of the tongue; the type 2 had a smooth and thick body, and was found in rear half of the tongue. 35 to 45 fungiform papillae were found on the dorsal surface of the tongue in both species. They were observed along the lateral margins and were also found on front and rear end part of the tongue. There were two to three noticeably large fungiform papillae arranged in a straight line on the region between lingual prominence and circumvallate papillae. There were two circumvallate papillae close to the rear end of the tongue. They were large and round, each having two layers of pads. The overall morphology of lingual papillae of M. schreibersi fuliginosus and P. savii was found to be similar with other Chiroptera. However, few but noticeable differences were found among the filiform papillae and fungiform papillae. Type 2 filiform papillae differed in that bifid and trifid configuration were found in M. schreibersi fulginosus unlike in P. savii. In addition, numbers of large fungiform papillae located in the center of posterior region of the tongue were different with M. schreibersi have three while P. savii having only two.

• Development and Reproduction
• /
• v.1 no.2
• /
• pp.133-139
• /
• 1997

To study the function and structure of Golgi apparatus in the spermiogenesis of long-fingered bat (Miniopterus schreibersi fuliginosus), the testis obtained from adult bat was treated with the prolonged osmification or fixed with ferrocyanide reduced osmium. golgi apparatus was oval shape in early Golgi phase, and was composed of cortex and medullar enclosing acrosome in mid Golgi phase. The vesicles of crescent shape Golgi apparatus were closed or fused with small or large vesicles at the periphery of acrosome. Golgi apparatus moved behing the acrosome face in cap phase, but the Golgi apparatus was still active. According to this, Golgi apparatus appears to be involved in the formation of acrosome and sperm tail. Transfer of materials from Golgi to acrosme seems to be carried out not only by fusion of large vesicles with acrosomal vesicles but also by detachment of coated vesicle from various cisternae of Golgi fusing with acrosomal vesicle.

• Biomedical Science Letters
• /
• v.10 no.4
• /
• pp.435-445
• /
• 2004

The characteristics of the testis and the annual cycle of the seminiferous epithelium of the Miniopterus schreibersi fuliginosus were examined by optical microscopy. The testis weight and diameter of the seminiferous tubules were increased gradually from May to July, and the highest activity was observed in August. The size then decreased rapidly from October. Spermatogenesis began in May, peaked in August, and was suspended from October to April in the following year. Spermatocytogenesis were produced from May to July. Spermiogenesis occurred from August to September. In particular, immature spematogenic cells in the seminiferous tubules were engulfed by the phagocytosis of Sertoli cells in October. From November to April, the seminiferous tubuly contained only Sertoli cells and Ad spermatogonia. Therefore, the periodic changes in the seminiferous epithelium of M. S. fuliginosus suggest that a long hibernation is an adaptive strategy for the preservation of energy and the regulation of the breeding cycle.

• 한국전자현미경학회:학술대회논문집
• /
• 1997.11a
• /
• pp.45-45
• /
• 1997

• Applied Microscopy
• /
• v.28 no.4
• /
• pp.603-613
• /
• 1998

The present study was designed in order to observe relationship between the endoplasmic reticulum and the Golgi complex during spermiogenesis of the long-fingered bat (Miniopterus schreibersi fuliginosus). The testes were obtained from adult bats and treated with the prolonged osmification or fixed with ferrocyanide reduced osmiun. In the Golgi phase, The Golgi complex shows an oval shape, and was composed of a cortex and a medullar enclosing acrosome. The Golgi vacuoles with electron-dense granules of crescent shape were fused with each other. The smooth endoplasrnic reticulum was scattered in all the area of the cytoplasm. In the cap phase, The Golgi complex was crescent in shape, and faced to a nucleus. Large and small vesicles were fused with each other, and then fused with a acrosomal vacuole. The rough endoplasmic reticulum was close to the large Golgi vacuole. In the acrosome phase, The Golgi complex was moved to behind of the acrosome face. Small vesicles were fused with an acrosome, and cisternae of the trans-face of Golgi complex was connected with an acrosome in the early acrosome phase. The smooth endoplasmic reticulum was distributed in the cytoplasm. The annulate lamellar was originated from a radial body-annulate lammellae complex. In the maturation phase, The Golgi complex with dilated cistrern appeared in the cytoplasm, and also, annulate lamellar was observed in the cytoplasm. The connection of the annulate lamellar with the cistern of radial body suggests that an annulate lamellar seems to be closely related to radial body. The smooth endoplasmic reticulum was scattered in the cytoplasm in the early Golgi phase, but annulate lamellar-radial body complex which might be a residual and disappearing form of the smooth endoplasmic reticulum appeared in the acrosome phase. The Golgi complex steadily remained in the late maturation phase when the endoplasmic reticulum began to disappear from the cytoplasm: the Golgi complex was still occurred after acrosome formation. The observations obtained in the present study, which was characterized by the presence of the Golgi complex in the late maturation phase, suggests that the Golgi complex may play an important role also even after the acrosome formation.

• The Korean Journal of Zoology
• /
• v.38 no.3
• /
• pp.405-416
• /
• 1995

The testis and the epididymis of sexually mature male bats were examined to investigate the process of spermiogenesis of Korean long-fingered bat (Miniopterus schreifersi fulignosus) using electron microscope. The ultrastructural findings were analysed on the basis of Lee's method (1992). Especially, we focused on the acrosome formation. The results are as follows: The spermiogenesis of the Korean long-fingered bat can be divided into ten phases on the basis of ultrastructural differentiation; three "Golgi" phases of early, mid and late stages, two "cap" and two "acrosome" phases respectively composed of early and late phases, one "maturation phase and two "spermiation" phases of early and late phases. The axoneme of sperm in the cauda epididymis is composed of nine outer dense fiber and a central singlet. The number 1, 5, 6, and 9 outer dense fibers are larger than others. In the Golgi phases, small vesicles are separated from Golgi vesicles and then appear to fuse into a large vesicle, and finally it contacd with the outerside of the nucleus. It suggests that proacrosomal material could be made in the cytoplasm before the Golgi vesicle formation and then it could be transferred into the Golgi vesicle and condensed more and more, and finally form acrosome, just as Lee;s suggestion (1992).m acrosome, just as Lee;s suggestion (1992).