• Title/Summary/Keyword: Microystis aeruginosa

Search Result 1, Processing Time 0.017 seconds

Axenic Isolation and 16S rRNA Gene Sequence of the Cyanobacterium Microcystis aeruginosa in Downstream of Nakdong River (낙동강 하류에 분포하는 남조류 Microcystis aeruginosa의 무균분리 및 16S rRNA 유전자 염기서열분석)

  • 박홍기;정은영;이유정;정종문;홍용기
    • Journal of Life Science
    • /
    • v.12 no.2
    • /
    • pp.158-163
    • /
    • 2002
  • For axenic isolation of the cyanobacterium Microcystis aeruginosa, water bloom at the Mulgum station from the Nakdong River was pretreated by shaking with distilled water. Removal of bacteria was accomplished using antibiotics (150 $\mu$g/$m\ell$ ampicillin and 25 $\mu$g/$m\ell$ neomycin) and colonizing on CB solid medium prepared from 0.7% agarose at 3$0^{\circ}C$ under 40 $\mu$ mol m$^{-2}$ s$^{-1}$ light. Among 26 strains of the Microcystis species, only three strains were axenically established. The three strains were examined by PCR-amplified 16S rRNA gene and 16S rRNA sequencing. The similarities were 99.5 ~100% with M. aeruginosa AF 139292.