• 농업과학연구
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• 제39권4호
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• pp.497-502
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• 2012

Since the year 2000, the production and consumption of broccoli have rapidly increased in Korea. And, the average production area and amount were about 1,700 ha and 29,000 ton for the past 5 years. Even with the increase of these cultivation and consumption, more than 95% of the broccoli seeds are currently imported from foreign countries such as Japan and Netherlands. Therefore, development of domestic broccoli varieties is needed to relieve Korean farmers' production cost for broccoli. In this situation, National Institute of Horticultural and Herbal Science (NIHHS) of Korea has tried to develop F1 hybrid varieties from elite lines that were obtained by microspore cultivation method from 2008. As the results, about 850 lines of broccoli were obtained and self-pollinated. Then their ploidy levels of the genome were confirmed to select double haploid (D.H.) lines. And the D.H. lines' horticultural traits were evaluated in open field. After the selection of 17 elite D.H. lines, they were cross-pollinated with a male sterile (MS) line to produce F1 hybrid seeds. After 2 to 3 years field trials of these F1 hybrid varieties at the area of Suwon, Gangneung, and Jeju respectively, two hybrid varieties such as 'Wongyo8011' and 'Wongyo8012' are selected for the application of variety protection. With these 4 years of research, we found that the microspore cultivation method is a powerful tool for the conventional breeding program, especially for the development of various inbred lines and even F1 hybrid varieties in short time.

• Journal of Plant Biotechnology
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• 제44권1호
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• pp.27-34
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• 2017

유전적으로 다양한 양배추 유전자원들의 소포자 배양을 통하여 배양에 효과적인 시기와 배양 조건을 구명하기 위해 본 연구를 실시하였다. 그 결과, '$2{\times}NLN$, $AgNO_3$ 1 mg/l, 13%의 sucrose'로 조성 된 배지 조건에서 배 생산에 효과적임을 확인 할 수 있었다. 배양 재료로 사용된 유전자원 중 소포자로부터 유도된 배 발생 개수는 일대잡종 세대 보다 자가 수정 후대인 $F_2$ 또는 $F_3$ 세대에서 높았다. 이러한 결과를 통하여 양배추의 일대잡종 품종에서 소포자 배양 시 배의 획득에 실패한 경우, 그의 자손 세대를 사용하여 소포자 배양을 지속하는 것을 추천한다. 그리고 소포자로부터 유도된 배와 식물개체 수는 개화 초기의 결과가 중기 및 말기 보다 높았다. 토양순화 된 식물체의 경우는 중기와 말기의 각각 27.0 % 및 1.8 %인 것에 비하여 개화 초기에 유도된 비율이 71.2 %였다. 따라서 소포자 배양에 활용할 꽃봉오리는 개화 초기에 채취하여 사용할 것을 권장한다.

• 식물조직배양학회지
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• 제27권5호
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• pp.411-413
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• 2000

The ability of microspores or young pollen grains (male gametophytes) to undergo developmetal switch to embryogenic (sporophytic) pathway exemplifies the concept of totipotency as applied to haploid posmeiotic cells. As a first step pollen is devoid of positional information provided in situ by the intact anther - by isolation and cultivation in vitro in artificial media. This is inevitably accompanied by some degree of stress response in microspore/pollen. It has been shown in both monocots and dicots that intentional stress treatment (mostly starvation or heat shock) greatly stimulates embryo induction rate. Using transgenic sHSP antisense Nicotiana tabacum we show that expression of small heat shock proteins is an integral part of successful embryo and later haploid plant production from pollen grains. Our recently published data show that sHSP chaperone function is optimal in the absence of ATP.