• Current Optics and Photonics
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• 제5권5호
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• pp.524-531
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• 2021

In an integral-imaging microscopy (IIM) system, a microlens array (MLA) is the primary optical element; however, surface errors impede the resolution of a raw image's details. Calibration is a major concern with regard to incorrect projection of the light rays. A ray-tracing-based calibration method for an IIM camera is proposed, to address four errors: MLA decentering, rotational, translational, and subimage-scaling errors. All of these parameters are evaluated using the reference image obtained from the ray-traced white image. The areas and center points of the microlens are estimated using an "8-connected" and a "center-of-gravity" method respectively. The proposed approach significantly improves the rectified-image quality and nonlinear image brightness for an IIM system. Numerical and optical experiments on multiple real objects demonstrate the robustness and effectiveness of our proposed method, which achieves on average a 35% improvement in brightness for an IIM raw image.

• Applied Microscopy
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• 제36권spc1호
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• pp.47-56
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• 2006

Improvements are made to existing primitive cell volume measurement method to provide a real-time analysis capability for the phase analysis of nanocrystalline materials. Simplification is introduced in the primitive cell volume calculation leading to fast and reliable method for nano-phase identification and is applied to the phase analysis of Mo-Si-N nanocoating layer. In addition, comparison is made between real-time and film measurements for their accuracy of calculated primitive cell volume values and factors governing the accuracy of the method are determined. About 5% accuracy in primitive cell determination is obtained from camera length calibration and this technique is used to investigate the cell volume variation in WC-TiC core-shell microstructure. In addition to chemical compositional variation in core-shell type structure, primitive cell volume variation reveals additional information on lattice coherency strain across the interface.

• Current Optics and Photonics
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• 제6권2호
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• pp.161-170
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• 2022

Three-dimensional (3D) geometric models are introduced to correct vignetting, and a downhill simplex search is applied to determine the coefficients of a 3D model used in digital microscopy. Vignetting is nonuniform illuminance with a geometric regularity on a two-dimensional (2D) image plane, which allows the illuminance distribution to be estimated using 3D models. The 3D models are defined using generalized polynomials and arbitrary coefficients. Because the 3D models are nonlinear, their coefficients are determined using a simplex search. The cost function of the simplex search is defined to minimize the error between the 3D model and the reference image of a standard white board. The conventional and proposed methods for correcting the vignetting are used in experiments on four inspection systems based on machine vision and microscopy. The methods are investigated using various performance indices, including the coefficient of determination, the mean absolute error, and the uniformity after correction. The proposed method is intuitive and shows performance similar to the conventional approach, using a smaller number of coefficients.

• 한국가시화정보학회지
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• 제10권2호
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• pp.25-31
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• 2012

High-performance smartphones, equipped with a digital camera and an application software, can render conventional bench-top laboratory instruments mobile at affordable costs. As the smartphone-based devices are portable and wireless, they have wide applications especially in providing point-of-care (POC) tests in resource-constrained areas. We developed a hand-held diagnostic system, Mobile SmartScope, which consists of a small optical unit integrated with a smartphone. The performance of the SmartScope was favorably compared with that of conventional light microscopy in detecting and quantifying red blood cells. We also evaluated the fluorescence detection limit of the SmartScope incorporated with a blue light-emitting diode and appropriate optical filters by using fluorescently labeled microbeads for intensity calibration.