• Title/Summary/Keyword: Microscopic Observation

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Variation of Tow Geometry according to Mold Property and Shear Angle during Draping on 3D Curved Surfaces (3 차원 곡면 드레이핑 중 금형의 물성과 전단각에 따른 토우구조의 변화)

  • Chung, Jee-Gyu;Chang, Seung-Hwan
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2005.04a
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    • pp.154-157
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    • 2005
  • This paper aims to investigate the tow deformation pattern with respect to shear angle and mold property during draping of plain weave carbon/epoxy prepreg. Aluminum and PVC foams with different foam density are used for the draping hemisphere molds with 250 mm diameters. Microscopic observation reveals that tow parameters like crimp angle and Y-directional tow intervals are influenced by shear angle and mold density at the same time. The correlation between crimp angle and Y-directional tow interval is also found out.

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Morphological and Anatomical Evaluation of Grafted Pinus merkusii

  • Susilowati, Arida;Iswanto, Apri Heri;Wahyudi, Imam;Supriyanto, Supriyanto;Siregar, Iskandar Z
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.6
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    • pp.903-912
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    • 2016
  • Morphological and anatomical evaluation of grafted P. merkusii have been undertaken to obtain the information about compatible and incompatible symptoms of 18 years old grafts based on morphological observation and microscopic analysis. Samples of compatible and incompatible grafts were obtained from previous research conducted by the Silviculture Departement Team in 1994. Result showed that compatible grafts have normal stem form and secondary growth (diameter growth), but some abnormality symptoms like undulated pattern of annual growth rings, phloem thickening and abnormality resin ducts in inner and middle parts of the union area occurred. Incompatible ones showed abnormality of the stem form, cortex-bark necrosis and swelling in the union area. Microscopic observation showed abnormality of all parts of the union, undulated pattern of annual growth rings, phloem thickening, abnormal resin ducts, low numbers and discontinuity of vascular elements in the union area.

The Effect of Dammarane Glycosides of Panax ginseng on Primary Cultured Chicken Brain Cells (인삼 Dammarane Glycoside류 분획물이 일차배양한 계배의 뇌세포에 미치는 영향)

  • Park, Mi-Jung;Song, Jin-Ho;Kim, Young-Choong
    • YAKHAK HOEJI
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    • v.33 no.1
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    • pp.39-45
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    • 1989
  • Effects of dammarane glycosides of Panax ginseng on primary cultured chicken embryonic brain cells were studied by microscopic observation and determination of the activity of pyruvate dehydrogenase complex (PDHC). Brain cells were prepared from the brain of 10-day-old chicken embryo and cultured with either a standard medium consisted of 85% Dulbecco's Modified Eagle Medium (DMEM), 10% horse serum and 5% chicken embryonic extracts or a deficient medium consisted of 90% DMEM and 10% horse serum. It was observed that dammarane glycosides of Panax ginseng seemed to show the tendency to stimulate the neurite outgrowth of brain cells which were cultured with a deficient medium under microscopic observation. The activity of PDHC in brain cells cultured with a deficient medium was increased by dammarane glycosides of Panax ginseng.

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Effects of Rock Weathering on the Degradation of Engineering Properties (암반풍화도에 따른 지질공학적 특성 저감효과)

  • Lee Chang-Sup;Cho Taechin
    • Tunnel and Underground Space
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    • v.15 no.6 s.59
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    • pp.411-424
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    • 2005
  • Weathering is defined as a process by which surface rock, once formed in the deep ground, is broken down and altered to keep the equilibrium with the ambient environment. In this study granitic rock samples of different weathering grades were collected in the field and the microscopic observation, X-ray diffraction analysis, electron microscopic observation, chemical analysis, and rock property tests were carried out. Formation of secondary minerals, especially clay minerals, by weathering was identified and the mechanism for the change of engineering properties such as rock strength degradation was analyzed. Tunnel model test, Failure behaviour, Shallow tunnel, Unsupproted tunnel length.

Electron Microscopic Observation of Liposomes with Negative Stain (Liposomes의 음성염색에 의한 전자현미경적 관찰)

  • Ryu, Yong-Wun;Hong, Seong-Woon;Kim, Tae-Hwan
    • Applied Microscopy
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    • v.19 no.2
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    • pp.1-16
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    • 1989
  • To investigate the research of the tumor seeker using liposomes with negatively stained. The fine structure and size in liposomes composed of PC, DSPC, DAPC and SM phospholipid were observed. And the yield of vesicle affected with sonication by mechanical force were determined. The results were as follows. 1. The stain of 2% UA are obtained a good resolution from electron microscopic observation to compare the negatively liposomes with PTA, AM and UA solution. 2. The fine structure of liposomes except DAPC, PC and CH alone samples could be observed from EM experiment of liposomes composed of PC, DSPC, DAPC and SM phospholipid. 3. The results of experiment from 10, 20, 40 and 60 times sonication with 30sec, prepared 0.1mM NTA-SM-liposomes were obtained unilamella vesicles from groups of multilamella vesicles of phospholipid. 4. About 50nm diameter liposomes obtained through membration filtration step to prepare homogenized liposomes.

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Studies on the Effect of Several Crude Drugs on Cultured Chicken Brain Cells (수종 생약이 일차배양한 계배의 뇌세포에 미치는 영향)

  • Park, Mi-Jung;Song, Jin-Ho;Kim, Young-Choong
    • Korean Journal of Pharmacognosy
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    • v.20 no.1
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    • pp.32-36
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    • 1989
  • Effects of Lycium chinensis, Epimedium koreanum and tuguaconitine which is isolated from Aconitum sibiricum on primary culture chicken embryonic brain cells were studied by microscopic observation and determined of the activity of pyruvate dehydrogenase complex(PDHC). Brain cells were prepared from the brain of 10-day-old chicken embryo and cultured with a medicine consisted of 90% Dulbecco's Modified Eagle Medium(DMEM) and 10% horse serum. It was observed that all substances studied seemed to show the tendency to stimulate the neurite outgrowth of brain cells which were cultured with a deficient medium under microscopic observation. The activity of PDHC in brain cells cultured with a deficient medium was increased by Lysium chinensis and Epimedium koreanum. However, tuguaconitine had not influence on the activity of PDHC.

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STUDIES ON EPIDERMAL-DERMAL SEPARATION AND ENZYME ACTIVITIES IN NEONATAL RAT EPIDERMIS

  • Kim, Hyeyoung
    • Toxicological Research
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    • v.5 no.2
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    • pp.89-96
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    • 1989
  • Rapid and complete epidermal-dermal separation procedure were determined in neonatal rat skin by light microscopic observation and by compairing enzyme activities in the separated epidermis. Microscopic appearance demonstrated the at four different separation procedures used in the study resulted in good separation of epidermis from dermis` heating method (i.e., immersion in 55C water for 30 sec, followed by immersion in 0-4C water) and microwave irradiation for 10 sec were saving time.

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Development of methodology for evaluating tribological properities of Ion-implanted steel (이온 주입한 강의 미시적 마모 튼성의 평가)

  • MOON, Bong-Ho;CHOI, Byung-Young
    • Journal of the Korean Society for Precision Engineering
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    • v.14 no.9
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    • pp.146-154
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    • 1997
  • Ion implantation has been used successfully as a surface treatment technology to improve the wear. fatigue and corrosion resistances of materials. A modified surface layer by ion implantation is very thin(under 1 m), but it has different mechanical properties from the substrate. It has also different wear characteristics. Since wear is a dynamic phenomenon on interacting surfaces with relative motion, an effective method for investigtating the wear of a thin layer is the observation of wear process in microscopic detail using in-situ system. The change of wear properties produces the transition of wear mode. To know the microscopic wear mechanism of this thin layer, it is very important to clarify its microscopic wear mode. In this paper, using the SEM and AFM Rribosystems as in-situ system, the microscopic wear of Ti ion-implanted 1C-3Cr steel, a material for roller in the cold working process, was investigated in repeated sliding. The depth of wear groove and the speciffc wear amount were changed with transition of microscopic wear mode. The depth of wear groove with friction cycles in AFM tribosystem and specific wear amount of Ti ion-implanted 1C-3Cr steel were less about 2-3 times than those of non-implanted 1C-3Cr steel. The microscopic wear mechansim of Ti ion-implanted 1C-3Cr steel was also clarified. The microscopic wear property was quantitatively evaluated in terms of microscopic wear mode and specific wear amount.

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Application on Microwave Energy in the Preparation of Fish Samples for Electron Microscopic Observation

  • Kim Soo Jin;Oh Hae Keun;Song Young-Hwan;Chung Hyun-Do;Kim Young-Tae;Park Nam-Kyu;Choi Tae-Jin
    • Fisheries and Aquatic Sciences
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    • v.1 no.2
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    • pp.187-191
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    • 1998
  • Chemotherapy can not be applied for the control of fish viral diseases because viruses depend on host machinery for their replication. Although new control strategies including vaccination are under development, avoidance of virus introduction by rapid and correct diagnosis is the best way of fish viral disease control. Although observation of virus particles with an electron microscope is an easy method for virus detection, it take a few days for the sample preparation. In order to shorten the sample preparation time, microwave radiation was applied in the procedure. With this method, 15 seconds was enough for fixation of virus infected fish samples or cultured cells inoculated with infectious hematopoietic necrosis virus, which takes 2-4 hours with routine methods. Also four minutes was enough for polymerization of embedding resin which takes 24-48 hours with routine methods. Samples prepared with microwave were good enough for direct electron microscopic observation and immunogold labeling assay.

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