• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.48 no.3
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• pp.107-114
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• 2016

Terra Alba Wallpaper was manufactured by coating inorganic terra alba paint on white base paper, and the surface of the coated layers were microscopically observed. Eco Terra Alba used the functional wall paper in this study was prepared by mixing inorganic Terra Alba and calcium carbonate. Terra Alba showed particle shape. The concentration of inorganic components, pH, and the viscosity of the coating solution were 92.0%, 7.82, 1286 cPs, respectively. The bar coater whit No 3 wire resulted in the least thickness of wet coating layer and coating thickness such $22.5{\mu}m$ with covered all the surface. Bar coater with No. 12 wire leaded to $48.4{\mu}m$ thickness. The coating layers above $43{\mu}m$ thickness showed the cracks on the wall paper surface after coating.

• Herbal Formula Science
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• v.15 no.2
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• pp.119-126
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• 2007

Objectives: This study presents observation of microorganism such as total aerobic bacteria, total fungus, E. coli, Pseudonomas aerugjnosa, Staphylococcus aureus, and Salmonella typhimurium in herbal decoction manufactured by Korean medical clinic. Methods: We examined to observe microorganism using the requirements for the experimental methods recommended by FDA. For the identification, we observed microscopic methods and carried out polymerase chain reaction (PCR) and DNA purification. The purified DNA samples were analyzed by DNA sequencer. As compared with NCBI database. the results were identified by sequences similarity. Results and conclusion: 26 (55%) of 46 decoctions observed positive for microbial test. 12 (46 %) of 26 positive decoctions exceed requirement of microbial limit test. These microbial colony identified genus of Bacillus using microscopic and DNA sequencing methods.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2003.10a
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• pp.54-54
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• 2003

We have developed a Cu-based bulk amorphous composite reinforced with a micron-sized crystalline phase, the (Cu60Zr30Ti10)95Ta5 amorphous matrix composite. The composite demonstrates the ultimate strength of 2332 MPa with a dramatically enhanced fracture strain of 15.3 %. Macroscopic observation of the fractured (Cu60Zr30Ti10)95Ta5 amorphous matrix composite showed the development of multiple shear bands along with numerous branching and deflection of shear bands. Microscopic observation on the amorphous matrix of the composite showed that cracks propagate through the residual amorphous matrix located between nanocrystallites, which had formed during deformation. Simulations based on finite element method were conducted to understand the formation mechanisms of multiple shear bands, the initiation site of shear bands, and interaction of shear bands with crystalline particles. Other microscopic fracture mechanism responsible for the enhanced plasticity was discussed.

• The Plant Pathology Journal
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• v.29 no.4
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• pp.465-470
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• 2013

In January 2012, spreading type petunia cv. Wave Pink plants showing an abnormal growth habit of sprouting unusual multiple plantlets from the lateral buds were collected from a greenhouse in Gwacheon, Gyeonggi Province, Korea. The presence of phytoplasma was investigated using PCR with the primer pairs P1/P6, and R16F1/R1 for nested-PCR. In the nested PCR, 1,096 bp PCR products were obtained, and through sequencing 12 Pet-Stol isolates were identified. Comparison of the nucleotide sequences of 16S rRNA gene of the 12 Pet-Stol isolates with other phytoplasmas belonging to aster yellows or Stolbur showed that Pet-Stol isolates were members of Stolbur. The presence of phytoplasma in petunia was also confirmed by microscopic observation of the pathogens. In this study, Stolbur phytoplasma was identified from spreading type petunia cultivars by sequence analysis of 16S rRNA gene of phytoplasma and microscopic observation of phytoplasma bodies. This is the first report of Stolbur phytoplasma in commercial Petunia hybrida cultivars.

• YAKHAK HOEJI
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• v.33 no.3
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• pp.161-166
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• 1989

Effects of dammarane glycosides of Panax ginseng on primary cultured chicken embryonic skeletal muscle cells were studied by microscopic observation and determination of the activity of acetylcholinesterase. Muscle cells were prepared from the breast of 12-day-old chicken embryo and cultured with either a medium consisted of 87.5% Dulbecco's Modified Eagle Medium (DMEM), 10% horse serum and 2.5% chicken embryonic extract or a medium consisted of 90% DMEM and 10% horse serum. It was observed that dammarane glycosides of Panax ginseng seemed to show the tendency to stimulate the growth and the differentiation of the muscle cells cultured with a medium consisted of 90% DMEM and 10% horse serum under microscopic observation. The activity of acetylcholinesterase in the muscle cells cultured with a medium consisted of 90% DMEM and 10% horse serum was increased by dammarane glycosides of Panax ginseng.

• Corrosion Science and Technology
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• v.7 no.1
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• pp.35-39
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• 2008

We have been studying application of electrochemical noise (Fluctuation) analysis for localized corrosion. Foils of Zinc, Aluminum and Magnesium were used as specimens for electrochemical cell simulating localized corrosion. These specimens were dipped in sodium chloride solutions adjusted to each exponent of Hydrogen ion concentration (pH) condition of 5.5, 10, 12 respectively. Time variations of potential and current were measured in those solutions, and simultaneously the surfaces of specimens were observed using microscope with television monitor. Two types of electrochemical cells were arranged for experiments simulated localized corrosion. The fluctuations on trendy component of short-circuited potential and short-circuited current were appeared in synchronization. It was seemed that these fluctuations result from hydrogen evolution on the aluminum active site in the crevice from the microscopic observation. In the case of zinc and magnesium, fluctuations appeared on the trendy component of the corrosion potential. Two types fluctuation were detected. First one is the fluctuation varied periodically. The second one is the random fluctuation. It was seemed that these fluctuations result from generation of corrosion products and hydrogen evolution on the active site in the crevice of zinc and magnesium from the microscopic observation.

• Journal of Microbiology and Biotechnology
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• v.6 no.6
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• pp.468-473
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• 1996

The genome of tomato spotted wilt virus (TSWV) is composed of three RNA segments, S, M, and L RNA and the 5.0 kb M RNA encodes two glycoproteins Gl, G2 and NSm protein of unknown function. In an effort to investigate the function of the NSm protein, antibody was raised against NSm fusion protein overexpressed in Escherichia coli. This antibody was used to detect the NSm protein by using western blot analysis and electron microscopic observation after immunogold labelling. For the cloning of the NSm gene, total RNA extracted from a TSWV infected plant was used for cDNA synthesis and polymerase chain reaction (PCR) instead of going through time-consuming virus purification. A protein band specifically reacting to the NSm antibody was detected from TSWV inoculated plants. The NSm protein was detected in the cell wall fraction and in pellet from low speed centrifugation when the infected plant tissue was fractionated into 4 fractions. In the immuno-electron microscopic observation, gold particles were found around the plasmodesmata of infected plant tissue. These results suggest that the NSm protein of TSWV plays some role in cell-to-cell movement of this virus.

• Journal of Convergence for Information Technology
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• v.9 no.6
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• pp.211-217
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• 2019

In this study, the change of skin condition was analyzed by skin analysis equipment using scrub cosmetics, and the morphological characteristics of microbead were observed by dermascope and scanning electron microscope. In the dermascope observation, during the process of cleansing, the microbeads attached to the skin existed in close contact with each other or dispersed. The skin after scrubbing was clean and smooth and the fine wrinkles between epidermal keratinocytes were reduced. In the scanning electron microscopic observation, the microbead surface did not have severe bending or rough surface. The skin moisture and oil content were higher than the scrub skin before the scrub, and there was no significant difference in the pH.

• Journal of fish pathology
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• v.36 no.2
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• pp.303-310
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• 2023

Yellow goosefish (Lophius litulon) is one of the important commercial fisheries target species in Korea, and commonly consumed as braised or stew. The microsporidian Spraguea is known to infect the nervous system of lophiid fish, forming numerous visible whitish xenomas. This parasite is commonly found in lophiid fish worldwide, but there is no information on the infection status of this parasite in Korea. We obtained commercially available chopped packs of lophiid fish from several fish markets and investigated their prevalence of infection. The isolated xenomas were crushed and purified as mature spore suspension. Microscopic observation and PCR were conducted to visualize and identify them. The host fish was also identified by DNA bar cording analysis. All the specimens were heavily infected and microscopic observation with Giemsa or Chromotrope 2R stain revealed tiny oval shapes of typical microsporidian spores. PCR analysis targeting the partial SSU rDNA showed that our specimen belongs to the genus Spraguea clade. But clear identification at the species level was not possible, due to the insufficient information of gene sequences available in GenBank. In addition, all of our host fish specimen was identified as yellow goosefish. This is the first report of a microsporidian parasite Spraguea infection in yellow goosefish from Korea.

• The Korea Journal of Herbology
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• v.20 no.2
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• pp.77-82
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• 2005

Objectives : A morphological classification among Achyranthes japonica Nakai (produced in Korea), A. bidentata Blume (imported from China), and Cyathula officinalis Kuan (used in China exclusively) was made through microscopic observation. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Result : 1. Korean A. japonica Nakai has slim roots, whose diameter is $1{\sim}5\;mm$. 1) Most of its intersection is stele, which includes a lot of vascular bundles. The inside of vascular bundles shows the arrangement of $2{\sim}4$ cycles, the innermost wheel of which is divided into two parts. 2) Parts of parenchymatous cell include Crystal sand of calcium oxalate. 2. The diameter of the roots of Chinese A. bidentata Blume is $0.4{\sim}1\;cm$. 1) The xylem in the vascular bundle in the middle of its intersection is rather big, around which the inner parts of the vascular bundles are arranged in the shape of $2{\sim}4$ cycles around. The most outside part of it is small, and the middle part is gathered into $2{\sim}3$ groups. 2) Parts of parenchymatous cells include Crystal sand of calcium oxalate. 3. The roots of Chinese C. officinalis Kuan is thick, whose diameter is $0.5{\sim}3\;cm$. 1) Vascular bundle of its intersection is spotted, arranged in the shape of $4{\sim}11$ concentric circles, whose center is divided into $2{\sim}9$ groups. 2) Parenchymatous cells include crystal sand and square crystal of calcium oxalate. Conclusion : It is concluded that Achyranthes japonica Nakai, A. bidentata Blume, and C. officinalis Kuan have different shapes in both external forms and internal intersections, so that they can be easily distinguished from one another through microscopic observation.