• Applied Microscopy
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• v.21 no.1
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• pp.46-62
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• 1991

The intermediate filament is one of the most important constituents of the intracytoplasmic cytoskeleton microtubule, actin, myosin and intermediate filament. It is composed of keratin, desmin, vimentin, neurofilament and glial filament, and has important role as a cellular marker, epithelial or mesenchymal origin. So it will be important to differentiated from some poorly or undifferentiated neoplasm to provide adequate therapeutic modalities. This study was performed by using immunohistochemical staining and electron microscopic observation to find out intermediate filaments of epithelial and non-epithelial tumor cells evaluate the degree of differentiation in tumors and therefore to provide some diagnostic and therapeutic modalities. The materials consisted of 83 epithelial and non-epithelial elements bearing 23 normal control, 28 epithelial tumors, and 32 non-epithelial tumors, that are resected for definite treatment at Chosun University Hospital from June, 1988 to June, 1990. Immunohistochemical stain for keratin, desmin and vimentin, and electron microscopic study were performed in all cases. The results obtained were as follows. 1. Immunohistochemical stain for intermediate filament were very useful diagnostic aid for differentiated epithelial tumor to non-epithelial tumor in diagnostic neoplasia. 2. In the electron microscopic finding, the size of intermediate filaments were possible differentiated to cell components of epithelial tumor and non-epithelial tumors.

• 한국문화재보존과학회:학술대회논문집
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• 2003.03a
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• pp.31-31
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• 2003

• 한국전자현미경학회:학술대회논문집
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• 2005.11a
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• pp.150-152
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• 2005

• Proceedings of the Korean Vacuum Society Conference
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• 2006.02a
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• pp.73-73
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• 2006

• The Journal of the Korean dental association
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• v.12 no.10
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• pp.763-766
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• 1974

The electron microscopic observation on the browny discolored enamel spot on smooth surface of permanent teeth was performed by mean of the Filmy Replica method and obtained the following: 1) The browny discolored enamel spot on smooth surface of permanenet teeth was identified as a dental caries.

• Korean Journal of Environmental Biology
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• v.36 no.2
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• pp.165-173
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• 2018

We sampled vomit of black-faced spoonbills(Platalea minor) during the brood-rearing season (from June 2011 to June 2014) at the Namdong reservoir in Incheon and analyzed the food components in the vomit using microscopy and next-generation sequencing (NGS). Microscopic observations primarily helped in identifying osteichthyes (bony fishes), crustaceans, and polychaetes. In particular, species belonging to the families Mugilidae and Gobiidae among the fish, and Macrophthalmus japonicas among the crustaceans, were observed at high frequency. Results of NGS analysis revealed the predominant presence of bony fish (42.58% of total reads) and crustaceans (40.75% of total reads), whereas others, such as polychaetes (12.66%), insects (0.24%), and unidentified species (3.78%), occurred in lower proportions. At the species level, results of NGS analysis revealed that Macrophthalmus abbreviates and Macrobrachium sp. among the crustaceans, and Acanthogobius hasta, Tridentiger obscurus, and Pterogobius zacalles among the bony fish, made up a high proportion of the total reads. These food species are frequently found at tidal flats in the Songdo and Sihwa lakes, emphasizing the importance of these areas as potential feeding sites of the black-faced spoonbill. Feed composition of the black-faced spoonbill, as evaluated by analyzing its vomit, differed when the evaluations were done by microscopic observation or by NGS analysis. Evaluation by microscopic observation is difficult and not error free, owing to the degradation of the samples to be analyzed; however, NGS analysis is more accurate, because it makes use of genetic information. Therefore, accurately analyzing food components from morphologically indistinguishable samples is possible by using genetic analysis.

• Applied Microscopy
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• v.10 no.1_2
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• pp.41-51
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• 1980

Scanning electron microscopic observation of the oviductal epithelium was carried out on the virgin white Wistar rat during normal sexual cycle, and obtained the results as follows; Rat oviductal epithelium during the normal sexual cycle generally consisted of two types of cells: ciliated and non-ciliated cell. The ciliated cells had cilia which was measured $0.2{\mu}$ in width and $3-5{\mu}$ in length. According to the length of microvilli and the distribution in each segment, the non-ciliated cell was further subdivided into four types of cells; NC-I, NC-II, NC-III, and NC-IV. The morphologic characteristics of each type of cells were also ascertained by SEM observations.

• Korean Journal of Veterinary Research
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• v.29 no.2
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• pp.99-108
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• 1989

In order to observe the pathological changes of kidney in rabbits infected with the viral haemorrhagic disease, the kidney tissues from the 91 rabbits infected with the viral haemorrhagic disease were examined by light and electron microscopy. The results observed were as follows: 1. On light microscopic observation, the kidney lesions were identified as haemorrhagic glomerular necrosis(33.0%), membranous glomerulonephritis(20.9%), thrombotic glomerulopathy(19.8%), membranoproliferative glomerulonephritis(8.8%), mesangial proliferative glomerulonephritis(8.8%) ischemic acute tubular necrosis(7.7%), and acute serous glomerulitis(6.6%). 2. On electron microscopic observation, cytoplasmic degeneration of mesangial cells, and irregular thickening of basement membranes with electron dense granular materials were observed. In podocytes swelling of mitochondria, dilatation of endoplasmic reticulum and extensive fusion of foot processes were also observed. Nonenveloped round icosahedral picornaviral particles with a diameter of 28~33nm were detected in the cytoplasm of degenerative endothelial cells, polymorphonuclear leucoytes, and monocytes.

• Composites Research
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• v.17 no.1
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• pp.38-46
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• 2004

This paper aims to correlate the micro-mechanical behaviour of tow geometry with applied in-plane forces during deformation of dry woven carbon-fibre fabric. These in-plane forces lead to differences in tow reorganisation during deformation and so changes in the way in which 'lock-up' occurs. In this paper, deformation of micro-mechanical parameters such as tow interval, crimp angle, change in tow amplitude and wavelength are investigated. To observe the micro-deformation of the fabric structure, appropriate specimens from bias extension and biaxial tests are sectioned and observed under the microscope. It was found that different loading conditions cause geometric deferences in the tow architecture. The variation in deformed tow geometry with shear angle is fitted using a simple parametric model.

• Journal of Sericultural and Entomological Science
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• v.39 no.2
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• pp.146-153
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• 1997

The microsporidian infection with Nosema bombycis, reconfirmed its high virulence and transovarial tranmissibility, however, the characteristic symptom of the spots like pepper grains on the diseased larval skin was no more recognized by present varieties of the silkworm. Transovarial transmission rate detected from moth was above 90% in dead eggs or dead larvae in the rearing by mulberry leaves, 80% in the newly hatched larvae starved to death. Transovarially transmitted N. bombycis was easily observed from dead eggs and larvae, and were suggested an individual inspection of a few of dead eggs for detection of the pathogenic spores. The progeny population provided indicative factors on the sampling of predictive and corrective inspection. The higher concentration of N. bombycis spores included in the hindabdormal part of infected moth, applicative on the simple method of indivisual moth inspection. For the predictive inspection of growned 5th-instar larvae, N. bombycis infection was detectable without microscopic observation by the unique symptom of turbid milky-white spots on the silk gland. Inspection of the meconia artificially discharged from silkworm moth, was also succesful of microscopic observation before crossing, without killing or homogenize the moths. The results provided a basis of rational methods for the inspection of N. bombycis infection of the silkworm.