• Proceedings of the Korean Nuclear Society Conference
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• 2004.05a
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• pp.385-385
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• 2004

• Proceedings of the Korean Nuclear Society Conference
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• 2003.10a
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• pp.417.2-417.2
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• 2003

• Proceedings of the Korean Nuclear Society Conference
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• 2005.10a
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• pp.797-797
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• 2005

• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1079-1082
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• 2007

Chromosome microdissection and the reverse FISH technique is one of the most useful methods for the identification of structurally abnormal chromosomes. In particular, the laser microbeam microdissection (LMM) method allows rapid isolation of a target chromosome or a specific region of chromosomes without damage of genetic materials and contamination. Isolated chromosomes were directly amplified by the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), and then the FISH probes labeled with spectrum green- or spectrum red-dUTP were generated by nick-translation. Whole chromosome painting (WCP) probes were successfully generated from only 5 copies of the chromosome. With this method, we produced 24 WCP probes for each human chromosome. We also tried to characterize a marker chromosome, which seemed to be originated from chromosome 11 on conventional banding technique. The marker chromosomes were isolated by the LMM method and analyzed by reverse FISH. We elucidated that the marker chromosome was originated from the short arm of chromosome 5 ($5p11{\to}pter$). A fully automated and computer-controlled LMM method is a very simple laboratory procedure, and enables rapid and precise characterization of various chromosome abnormalities.

• Proceedings of the Korean Nuclear Society Conference
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• 2005.05a
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• pp.988-989
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• 2005

• Structural Engineering and Mechanics
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• v.46 no.3
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• pp.417-431
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• 2013

This paper presents responses of the free end of a cantilever micro beam under the effect of an impact force based on the modified couple stress theory. The beam is excited by a transverse triangular force impulse modulated by a harmonic motion. The Kelvin-Voigt model for the material of the beam is used. The considered problem is investigated within the Bernoulli-Euler beam theory by using energy based finite element method. The system of equations of motion is derived by using Lagrange's equations. The obtained system of linear differential equations is reduced to a linear algebraic equation system and solved in the time domain by using Newmark average acceleration method. In the study, the difference of the modified couple stress theory and the classical beam theory is investigated for the wave propagation. A few of the obtained results are compared with the previously published results. The influences of the material length scale parameter on the wave propagation are investigated in detail. It is clearly seen from the results that the classical beam theory based on the modified couple stress theory must be used instead of the classical theory for small values of beam height.

• Asian Journal of Atmospheric Environment
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• v.4 no.2
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• pp.106-114
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• 2010

By the application of novel double detector system of micro-PIXE that can detect light elements (Z<14), we made an attempt to provide a thorough discussion on the aging processes of Asian dust (hereafter called "AD") particle by reaction with sea-slat. The elemental spectra and maps obtained from the microbeam radiation of micro-PIXE to individual AD particles were useful for fractionating AD particles into both internally and externally mixed particles. A spatial distribution of elements in a minute domain of single particle obtained by scanning the microbeam irradiation enabled us not only to estimate the chemical mixing state of individual AD particles but also to presume their aging processes in both ambient air and cloud. By calculating the normalized micro-PIXE net count of elements, it was possible to classify individual AD particles into three distinct groups (i.e., (1) Aging type 1: AD particle coated by the gaseous Cl evaporated by the reaction between artificial acids and sea salt; (2) Aging type 2: AD particle mixed with sea salt but no additional reaction with artificial acids; and (3) Non-aged type) A relatively high transformation rate (63.3-75.9%) was shown in large particles (greater than $5.1\;{\mu}m$ in diameter).

• Structural Engineering and Mechanics
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• v.59 no.3
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• pp.579-599
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• 2016

In this study, static bending of edge cracked micro beams is studied analytically under uniformly distributed transverse loading based on modified couple stress theory. The cracked beam is modelled using a proper modification of the classical cracked-beam theory consisting of two sub-beams connected through a massless elastic rotational spring. The deflection curve expressions of the edge cracked microbeam segments separated by the rotational spring are determined by the Integration method. The elastic curve functions of the edge cracked micro beams are obtained in explicit form for cantilever and simply supported beams. In order to establish the accuracy of the present formulation and results, the deflections are obtained, and compared with the published results available in the literature. Good agreement is observed. In the numerical study, the elastic deflections of the edge cracked micro beams are calculated and discussed for different crack positions, different lengths of the beam, different length scale parameter, different crack depths, and some typical boundary conditions. Also, the difference between the classical beam theory and modified couple stress theory is investigated for static bending of edge cracked microbeams. It is believed that the tabulated results will be a reference with which other researchers can compare their results.

• Nuclear Engineering and Technology
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• v.40 no.7
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• pp.551-560
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• 2008

Radiation has stochastic aspects in its generation, its choice of interaction mode during traveling in media, and its impact on living bodies. In certain circumstances, like in high dose environments resulting from low-LET radiation, the variance in its impact on a target volume is negligible. On the contrary, in low dose environments, especially when they are attributed to high-LET radiation, the impact on the target carries with it a large variance. This variation is more significant for smaller target volumes. Microdosimetric techniques, which have been developed to estimate the distribution of radiation energy deposited to cellular and subcellular-sized targets, contrast with macrodosimetric techniques which count only the average value. Since cells and DNA compounds are the critical targets in human bodies, microdosimetry, or dose estimation by microscopic approach, helps one better analyze the biological effects of radiation on the human body. By utilizing microbeam systems designed for individual cell irradiation, scientists have discovered that human cells exhibit radiosensitive reactions without being hit themselves (bystander effect). During the past 10 or more years, a new therapeutic protocol using discontinuous multiple micro-slit beams has been investigated for its clinical application. It has been suggested that the beneficial bystander effect is the essence of this protocol.

• Structural Engineering and Mechanics
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• v.76 no.6
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• pp.765-779
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• 2020

This article presents a nonclassical size dependent model based on the modified couple stress theory to study and analyze the bending behavior of perforated microbeams under different loading patterns. Modified equivalent material and geometrical parameters for perforated beam are presented. The modified couple stress theory with one material length scale parameter is adopted to incorporate the microstructure effect into the governing equations of perforated beam structure. The governing equilibrium equations of the perforated Timoshenko as well as the perforated Euler Bernoulli are developed based on the potential energy minimization principle. The Poisson's effect is included in the governing equilibrium equations. Regular square perforation configuration is considered. Based on Fourier series expansion, closed forms for the bending deflection and the rotational displacements are obtained for simply supported perforated microbeams. The proposed methodology is validated and compared with the available results in the literature and an excellent agreement is detected. Numerical results demonstrated the applicability of the proposed methodology to investigate the bending behavior of regularly squared perforated beams incorporating microstructure effect under different excitation patterns. The obtained results are significantly important for the design and production of perforated microbeam structures.