• 보존과학회지
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• 제36권4호
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• pp.255-263
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• 2020

Paulownia coreana has various advantages as a convenient workability, aesthetic outlook, beautiful patterns, low weight and high strength, and its permeability. P. coreana has been widely used for storage but there are no empirical researches proving its functionality in a field of conservation science until now. In this study, the seasonal and daily temperature and relative humidity control, and microbe and insect repellent activity were evaluated under the controlled and uncontrolled circumstances from 2015 to 2016. The results showed to be mainly excellent in relative humidity control and the buffering effect was good to adjust the average daily relative humidity range from the outside. With respect to the antimicrobial properties of P. coreana, we observed that its water-soluble extract produced visible zones of inhibition against five bacteria. However, it was difficult to predict the antimicrobial and/or insecticidal properties.

• 대한한방부인과학회지
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• 제20권1호
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• pp.1-15
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• 2007

Purpose : This study was conducted to investigate the antimicrobial effects of herb for removing dampness and promoting urination against vaginal microbes. Methods : Staphylococcus aureus. Methicillin-resistant Staphylococcus aureus(MRSA), Candida albicans and Gardnerella vaginalis were used for vaginitis-induced microbes. Lactobacillus gasseri, Streptococcus spp. and Escherichia coil HB101 were used for normal vaginal florae. And herbs for removing dampness and promoting urination(Dianthi herbs. Tokoro Rhizoma, Saururi Herbs, Pyrrosiae Folium, Artemisiae Iwayomogii Herba, Plantaginis Semen, Tetrapanacis Medulla, Polygoni Avicularis Herba, Malvae Semen, Akebiae Caulis, Kochiae Fructus, Lygodii Spora) were used. Antimicrobial activities were estimated by the change of optical densities and colony test in vitro. Results : Plantaginis Semen, Artemisiae Iwayomogii Herba and Lygodii Spora had the antimicrobial susceptibility and selective toxicity against MRSA and Gardnerella vaginalis. Polygoni Avicularis Herba had the antimicrobial susceptibility and selective toxicity against Staphylococcus aureus. MRSA and Gardnerella vaginalis. Malvae Semen and Kochiae Fructus had the antimicrobial susceptibility and selective toxicity against MRSA. Dianthi Herba had the antimicrobial susceptibility and selective toxicity against Gardnerella vaginalis. Conclusion : According to these results, we can suggest that Plantaginis Semen, Artemisiae Iwayomogii Herba, Lygodii Spora, Polygoni Avicularis Herba, Malvae Semen, Kochiae Fructus and Dianthi Herba would be available to the antimicrobial agent for vaginitis-induced microbe in vitro.

• 한국지하수토양환경학회지:지하수토양환경
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• 제21권1호
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• pp.94-103
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• 2016

Landfarming that supplies aerobic biodegradation condition to indigenous microbes in soils is a biological remediation technology. In this research, volatilization and biodegradation rate by indigenous microbes in the soil contaminated with total petroleum hydrocarbons (TPH) were measured. Soils were contaminated with diesel artificially and divided into two parts. One was sterilized by autoclave to remove indigenous microorganism and the other was used as it was. Various moisture contents and number of tillings were applied to the soil to find out proper condition to minimize volatilization and enhance bioremediation. Volatilization of TPH was inhibited and biodegradation was enhanced by increase on moisture content. Tilling was usually used to supply air for microbes, but tillings did not affect the growth of microbes in our study. Enough moisture content and proper aeration are important to control volatilization in landfarming. Also, TPH degradation was a function of the microbe counts (x₁), numbers of tilling (x₂), and moisture content (x₃) from the application of the response surface methodology. Statistical results showed the order of significance of the independent variables to be microbe counts > numbers of tilling > moisture content.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 춘계학술대회 및 임시총회
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• pp.54-54
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• 2015

Crops lack genetic resistance to most necrotrophic soil-borne pathogens and parasitic nematodes that are ubiquitous in agroecosystems worldwide. To overcome this disadvantage, plants recruit and nurture specific group of antagonistic microorganisms from the soil microbiome to defend their roots against pathogens and other pests. The best example of this microbe-based defense of roots is observed in disease-suppressive soils in which the suppressiveness is induced by continuously growing crops that are susceptible to a pathogen. Suppressive soils occur globally yet the microbial basis of most is still poorly described. Fusarium wilt, caused by Fusarium oxysporum f. sp. fragariae is a major disease of strawberry and is naturally suppressed in Korean fields that have undergone continuous strawberry monoculture. Here we show that members of the genus Streptomyces are the specific bacterial components of the microbiome responsible for the suppressiveness that controls Fusarium wilt of strawberry. Furthermore, genome sequencing revealed that Streptomyces griseus, which produces a novel thiopetide antibiotic, is the principal species involved in the suppressiveness. Finally, chemical-genetic studies demonstrated that S. griseus antagonizes F. oxysporum by interfering with fungal cell wall synthesis. An attack by F. oxysporum initiates a defensive "cry for help" by strawberry root and the mustering of microbial defenses led by Streptomyces. These results provide a model for future studies to elucidate the basis of microbially-based defense systems and soil suppressiveness from the field to the molecular level.

• 한국응용과학기술학회지
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• 제26권1호
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• pp.51-59
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• 2009

Natural Artemisia extraction was extracted from Artemisia component using diethyl ether as a solvent, and we tested various pharmacetical and chemical characteristics of this extract. Characteristic experiments to use natural Artemisia extract tested antimicrobial experiment using microbe in pharmacetical material, and tested dye experiment using fiber in chemical material. From the result of characteristics experiment, some conclusions are obtained as follow. From the result of extraction experiment, it obtained about 10.4%-Artemisia extraction ratio as semi-solid state, and after dried in freezing from Artemisia extract of semi-solid state, it obtained about 10%-Artemisia extraction ratio as solid state of dark blue-green color. From result of antimicrobial experiment of Artemisia extract, number of staphylococcus aureus (ATCC-01) and aspergillus niger (ATCC-02) in microbe decreased more and more according to time passage. This phenomenon showed that Artemisia extract influences to antimicrobial effect. From the result of dye experiment of Artemisia extract, it appeared in direction of dark blue-green color after dyed to use cotton and silk with fiber to control in pH 7.5. Specially the result which confirmed dye of fiber with optical electron microscope (OEM), we could know that it appears darker silk than cotton.

• 폴리머
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• 제26권3호
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• pp.293-299
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• 2002

항균제인 m-크레졸을 아크릴로일클로라이드와 반응시켜 m-크레실아크릴레이트를 합성하고 이를 메틸메타크릴레이트와 아크릴산과 3성분 라디칼 공중합시켜 m-크레졸 moiety를 가지는 고분자를 합성하였다. 이들 고분자중의 m-크레실아크릴레이트단위는 핵자기공명스펙트라로부터 각각 4.7 mol%와 10.1 mol%로서 측정되었다. 이들 고분자의 수평균분자량은 12000∼15000이었다. 이들 고분자는 가수분해를 통해 m-크레졸을 방출하였고 방출되는 그 양은 pH증가에 따라 증가하였다. 이것은 고분자중의 아크릴산 단위의 카르복실기의 수소가 pH증가에 따라 해리하기 용이하기 때문인 것으로 설명된다. 아울러 이들 고분자는 양성균 S. aureus와 음성균 E. coli에 대해서 항균성을 나타내었다.

• 한국토양비료학회지
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• 제44권1호
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• pp.58-66
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• 2011

Arsenic pollution is a serious global concern which affects all life forms. Being a toxic metalloid, the continued search for appropriate technologies for its remediation is needed. Phytoremediation, the use of green plants, is not only a low cost but also an environmentally friendly approach for metal uptake and stabilization. However, its application is limited by slow plant growth which is further aggravated by the phytotoxic effect of the pollutant. Attempts to address these constraints were done by exploiting plant-microbe interactions which offers more advantages for phytoremediation. Several bacterial mechanisms that can increase the efficiency of phytoremediation of As are nitrogen fixation, phosphate solubilization, siderophore production, ACC deaminase activity and growth regulator production. Many have been reported for other metals, but few for arsenic. This mini-review attempts to present what has been done so far in exploring plants and their rhizosphere microbiota and some genetic manipulations to increase the efficiency of arsenic soil phytoremediation.

• 한국의류산업학회지
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• 제11권5호
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• pp.818-826
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• 2009

Dyeability of microbial violacein produced from Chromobacterum violaceum CV107 on to multifiber fabrics has been studied. The bluish-purple colourants were produced by cultivation of Chromobacterum violaceum using LB liquid medium for 2 days. The colourant was extracted with 80% acetone and identified as violacein by LC/MS analysis. The violacein could be dyed on not only natural fibers such as Cotton, Silk and Wool but also synthetic fibers such as Diacetate, Triacetate, Creslan 61 and Nylon 66. Maximum K/S values were shown at 540-580 nm according to different fiber with color appearance of purple or blue. An optimum pH and temperature under dyeing condition were 10 and $70^{\circ}C$, respectively. Any mordants were not improved colour density and quality on various fabrics. From this studies, pigments produced microbe have a high potentials for natural dyeing on fabrics. Finally, development of new colourants from microbe has made a possible change for new dyeing field in respects of eco-friend and repeatability of natural dyeing for apparels.

• 한국응용과학기술학회지
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• 제26권3호
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• pp.341-349
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• 2009

Natural grape extraction was extracted from grape component using diethyl ether as a solvent, and we tested various pharmaceutical and chemical characteristics of this extract. Characteristic experiments to use natural grape extract tested antimicrobial experiment using microbe in pharmaceutical material, and tested dye experiment using fiber in chemical material. From the result of characteristics experiment, some conclusions are obtained as follow. From the result of characteristics experiment, it obtained about 7.5%-grape extraction ratio as semi-solid state, and after dried in freezing from grape extract of semi-solid state, it obtained about 10%-grape extraction ratio as solid state of dark purple color. From result of antimicrobial experiment of grape extract, number of staphylococcus aureus (KCMC-01) and aspergillus niger (KCMC-02) in microbe decreased more and more according to time passage. This phenomenon showed that grape extract influences to antimicrobial effect. From the result of dye experiment of grape extract, it appeared in direction of dark purple color after dyed to use cotton and silk with fiber to control in pH 7.5. Specially the result which confirmed dye of fiber with optical electron microscope(OEM), we could know that it appears darker silk than cotton.

• 폴리머
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• 제27권5호
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• pp.443-448
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• 2003

항균제인 2-페닐페놀을 아크릴로일 클로라이드와 반응시켜 2-비페닐릴 아크릴레이트를 합성하고 이를 호제성분의 주요단량체인 메틸 메타아크릴레이트와 아크릴산과 3성분 라터칼 공중합시켜 2-페닐페놀 moiety를 가지는 공중합체를 합성하였다. 이들 고분자중의 2-비페닐릴 아크릴레이트 단위는 핵자기공명스펙트라로부터 각각 4.2 몰%와 9.1 몰%로서 측정되었다. 이들 고분자의 수평균 분자량은 15000∼16000이었다. 이들 고분자는 가수분해를 통해 2-페닐페놀을 방출하였고 방출되는 양은 pH 증가에 따라 증가하였다. 방출된 용액은 양성균 S. aureus 보다 음성균 E. coli에 대해 보다 높은 항균성을 나타내었다.