• The Korean Journal of Physiology and Pharmacology
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• v.26 no.6
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• pp.519-530
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• 2022

Recent research indicates that lactate promotes the switching of vascular smooth muscle cells (VSMCs) to a synthetic phenotype, which has been implicated in various vascular diseases. This study aimed to investigate the effects of lactate on the VSMC phenotype switch and the underlying mechanism. The CCK-8 method was used to assess cell viability. The microRNAs and mRNAs levels were evaluated using quantitative PCR. Targets of microRNA were predicted using online tools and confirmed using a luciferase reporter assay. We found that lactate promoted the switch of VSMCs to a synthetic phenotype, as evidenced by an increase in VSMC proliferation, mitochondrial activity, migration, and synthesis but a decrease in VSMC apoptosis. Lactate inhibited miR-23b expression in VSMCs, and miR-23b inhibited VSMC's switch to the synthetic phenotype. Lactate modulated the VSMC phenotype through downregulation of miR-23b expression, suggesting that overexpression of miR-23b using a miR-23b mimic attenuated the effects of lactate on VSMC phenotype modulation. Moreover, we discovered that SMAD family member 3 (SMAD3) was the target of miR-23b in regulating VSMC phenotype. Further findings suggested that lactate promotes VSMC switch to synthetic phenotype by targeting SMAD3 and downregulating miR-23b. These findings suggest that correcting the dysregulation of miR-23b/SMAD3 or lactate metabolism is a potential treatment for vascular diseases.

• Geomechanics and Engineering
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• v.13 no.1
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• pp.63-77
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• 2017

The failure mechanism of a deep hard rock tunnel under high geostress and high geothermalactivity is extremely complex. Uniaxial compression tests of granite at different temperatures were conducted. The complete stress-strain curves, mechanical parameters and macroscopic failure types of the rock were analyzed in detail. The brittleness index, which represents the possibility of a severe brittleness hazard, is proposed in this paperby comparing the peak stress and the expansion stress. The results show that the temperature range from 20 to $60^{\circ}C$ is able to aggravate the brittle failure of hard rock based on the brittleness index. The closure of internal micro cracks by thermal stress can improve the strength of hard rock and the storage capacity of elastic strain energy. The failure mode ofthe samples changes from shear failure to tensile failure as the temperature increases. In conclusion, the brittle failure mechanism of hard rock under the action of thermal coupling is revealed, and the analysis result offers significant guidance for deep buried tunnels at high temperatures and under high geostress.

• Journal of Information Technology Services
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• v.11 no.3
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• pp.49-65
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• 2012

This study aims to investigate the factors influencing followers' continuous usage intention of twitter based on Elaboration Likelihood Model. In particular, central route(information itself) and peripheral route(twitterian's trustworthiness) are tested to reveal which has a more influential role in effecting the continuous usage intention of power-twitter. Furthermore, this study tests the relationship of users' attitude and continuous usage intention. The results indicate that argument quality, accuracy(central route), source expertise and source trustworthiness (peripheral route) have an influence on attitude. However, trustworthy on central route is not significant. Argument quality, trustworthy, accuracy(central route) and source expertise(peripheral route) have an influence on perceived usefulness. But source trustworthiness on peripheral is not significant. Attitude has an influence on perceived usefulness, and attitude and perceived usefulness both have an influence on perceived usefulness. In conclusion, central route is more influential than peripheral route in forming a positive attitude towards arguments, which in turn leads to the perception of twitter's usefulness, and in the end brings the continuous usage intention of twitter.

• Particle and aerosol research
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• v.5 no.4
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• pp.171-178
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• 2009

$PM_{2.5}$ is one of critical air pollutants due to its high absorbability of heavy metallic fumes, PAH and bacillary micro organisms. Such a fine particulate matter is often formed through various nucleation processes including condensation. This study attempts to find the nucleation behaviors of $PM_{2.5}$ arisen from coal power stations using a classical heterogeneous Fletcher's theory. The numerical simulation by C-language could approximate the nucleation process of $PM_{2.5}$ from water vapor, of which approach revealed the required energy for embryo formation and embryo size and nucleation rate. As a result of the calculation, it was found that wetting agents could affect the particle nucleation in vapor condensation. In particular, critical contact angle relates closely with the vapor saturation. Particle condensation could be reduced by lowering the angles. The wetting agents aid to decrease the contact angle and surface tensions, thereby may contribute to save the formation energy.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.07a
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• pp.557-560
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• 2003

The submicron $YBa_2Cu_3O_x$ powder was prepared by the sol-gel method. The particle size is distributed from 0.2 to $1.0\;{\mu}m$, which benefits to eliminate the micro-cracks formed in the $YBa_2Cu_3O_x$ films deposited by electrophoresis. The powder was single phase of $YBa_2Cu_3O_x$ examined by X-ray diffraction. In the sol-gel process the citrate gel was formed from citric acid and nitrate solution of $Y_2O_3$, $Ba(NO_3)O_2$ and CuO. When pH values were adjusted to $6.4{\sim}6.7,\;Ba(NO_3)O_2$ could be dissolved in the citrate solution completely. Appropriate evaporative temperature of the sol-gel formation is discussed. After the heat treatment the transition temperature($T_c$) and critical current density($J_c$) of the $YBa_2Cu_3O_x$ samples made of the submicron powder were measured.

• Journal of the Speleological Society of Korea
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• no.76
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• pp.49-55
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• 2006

The submicron $YBa_2Cu_3O_x$ powder was prepared by the sol-gel method. The particle size is distributed from 0.2 to 1.0 ${\mu}m$, which benefits to eliminate the micro-cracks formed in the $YBa_2Cu_3O_x$ films deposited by electrophoresis. The powder was single phase of $YBa_2Cu_3O_x$ examined by X-ray diffraction. In the sol-gel process the citrate gel was formed from citric acid and nitrate solution of $Y_2O_3$, $Ba(NO_3)_2$ and CuO. When pH values were adjusted to 6.4-6.7, $Ba(NO_3)_2$ could be dissolved in the citrate solution completely. Appropriate evaporative temperature of the sol-gel formation is discussed. Acetone is used as electrophoreticsolution, in which some water and iodine (0.2 g/1) and polyethylene glycol (2 vol. %) are added. The concentrations of $YBa_2Cu_3O_x$ powders is 20g/l. The thickness of deposited film could be more than 50 ${\mu}m$ in 3 minutes of depositing time. The most EPD films could be 90K zero resistance and the Jc values were over 1000A/cm2 (0 H, 77 K).

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2005.07a
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• pp.260-261
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• 2005

$YBa_2Cu_3O_{7-\delta}$ superconductor films have been prepared on silver substrates by electrophoretic deposition. As silver does not react with $YBa_2Cu_3O_{7-\delta}$ compound and has little influence on its superconductivity, it is usually doped in $YBa_2Cu_3O_7$ to improve the strength of the material and eliminate micro-cracks. It has been proved that Ag additive can lower the melting temperature of $YBa_2Cu_3O_{7-\delta}$ and act as linking bridge among $YBa_2Cu_3O_{7-\delta}$ particles, thus in this paper Ag doped $YBa_2Cu_3O_{7-\delta}$ thick films are prepared by electrophoretic co-deposition. As there are only some referenced experience formula and models for co-electrophoretic deposition and does not exist unified explanation, the behavior of Ag particles during co-electrophoretic deposition is also studied.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.10
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• pp.1-7
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• 2020

In this study, a multichannel gas-liquid microextraction system is designed by integrating the automatic elution of extraction line and multichannel gas-purging liquid phase microextraction. The system uses an injection pump and inert gas to push the extraction solvent to a sample bottle of a gas-phase color autosampler and then implements multichannel gas-liquid microextraction and gas chromatography-mass spectrometry. The system also employs a three-way integrated micro-high-temperature heater, syringe pump, and microflow controller to realize the simultaneous processing of multiple groups of samples, thus improving the sample pretreatment speed and reproducibility and reducing human error. Autoinjection experiments were implemented with polycyclic aromatic hydrocarbon standard samples. The experiments show that the average recovery rate of the system exceeds 70%, and the relative standard among the channels is less than 15%.

• Advances in nano research
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• v.12 no.2
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• pp.167-183
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• 2022

In this paper, the free and forced vibration analysis of rotating cantilever nanoscale cylindrical beams and tubes is investigated under the external dynamic load to examine the nonlocal effect. A couple of nonlocal strain gradient theories with different beams and tubes theories, involving the Euler-Bernoulli, Timoshenko, Reddy beam theory along with the higher-order tube theory, are assumed to the mathematic model of governing equations employing the Hamilton principle in order to derive the nonlocal governing equations related to the local and accurate nonlocal boundary conditions. The two-dimensional functional graded material (2D-FGM), made by the axially functionally graded (AFG) in conjunction with the porosity distribution in the radial direction, is considered material modeling. Finally, the derived Partial Differential Equations (PDE) are solved via a couple of the generalized differential quadrature element methods (GDQEM) with the Newmark-beta techniques for the time-dependent results. It is indicated that the boundary conditions equations play a crucial task in responding to nonlocal effects for the cantilever structures.

• Advances in nano research
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• v.14 no.2
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• pp.141-154
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• 2023

Effects of viscoelastic foundation on vibration of curved-beam structure with clamped and simply-supported boundary conditions is investigated in this study. In doing so, a micro-scale laminate composite beam with two piezoelectric face layer with a carbon nanotube reinforces composite core is considered. The whole beam structure is laid on a viscoelastic substrate which normally occurred in actual conditions. Due to small scale of the structure non-classical elasticity theory provided more accurate results. Therefore, nonlocal strain gradient theory is employed here to capture both nano-scale effects on carbon nanotubes and microscale effects because of overall scale of the structure. Equivalent homogenous properties of the composite core is obtained using Halpin-Tsai equation. The equations of motion is derived considering energy terms of the beam and variational principle in minimizing total energy. The boundary condition is assumed to be clamped at one end and simply supported at the other end. Due to nonlinear terms in the equations of motion, semi-analytical method of general differential quadrature method is engaged to solve the equations. In addition, due to complexity in developing and solving equations of motion of arches, an artificial neural network is design and implemented to capture effects of different parameters on the inplane vibration of sandwich arches. At the end, effects of several parameters including nonlocal and gradient parameters, geometrical aspect ratios and substrate constants of the structure on the natural frequency and amplitude is derived. It is observed that increasing nonlocal and gradient parameters have contradictory effects of the amplitude and frequency of vibration of the laminate beam.