• Journal of Nutrition and Health
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• v.17 no.2
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• pp.94-100
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• 1984

The effect of methionine supplementation to the isolated soyprotein(ISP) diet on the growth, body metabolism and composition of the Albino male rats was studied. Three levels(0.3,0.6 and 0.9%) of methionine were supplemented to the ISP diet with the constant levels of energy and protein of 3,600 kcal/kg and 20%, respectively. The body weight and weight gain of the growing rats were significantly increased by 0.3% methionine supplementation to the ISP diet compared to the ISP diet(P< 0.05).The effects of methionine supplementation to the ISP diet tended to be larger with increasing of the level of methionine supplementation, 0.6 and 0.9%, were statistically insignificant. Food and gross energy intake of growing rats fed the ISP diet or the ISP supplemented with methionine diet were lower than those fed the casein diet(P< 0.05). FER and PER of all the methionine supplemented diets were higher than those of the ISP or casein diet (P< 0.05) without significant differences among the supplementation levels of methionine to the diets. The weight gain of adult rats fed 0.9% methionine supplemented ISP diet were higher than those of the other treatments with significant difference. The effects of methionine supplementation to the ISP diet on the protein digestibility, BV, NPU, N-balance, N-retention, and body and liver compositions were not significant.

• Journal of Nutrition and Health
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• v.1 no.3_4
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• pp.197-200
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• 1968

Using the $Methionine-2-C^{14}$, the metabolism of methionine to the experimental rats on rice diet was studied comparing with that to the rats on stock diet in this paper. The National Institute of Health strain of weaning albino rats were housed into the individual cages deviding into 2 groups, the rice diet (RD) group and the stock diet (SD) group, and fed on rice diet and stock diet respectively for 10 weeks. On the day of experiment, the rats were parenterally administered. the $methionine-2-C^{14}$ solution after fasting over night. And then the rats were sacrificed by ether anesthesia by time being of one, three, six, and twelve hours each and the organs, pituitary gland, pancreas, spleen, liver, and kidney, were taken out for the determinations of radioactivities. And also the excretion of radioactivities through urine were determined by time being. The radioactivities were determined by Autoscaler SC-51 using the planchets. The results of radioactivities of urine excretion were shown at table 3 and the results of radioactivities distibutions in the organs by time being after parenteral administration of $methionine-2-C^{14}$ were shown table 4 in the original paper. According to the results, the following are summarized; 1. The growth experiment result of rats on. rice diet and stock diet were same as shown by the previous workers indicating significant growth inhibition at the rice diet group. 2. Due to the result of radioactivity excretion through urine after administration of $methionine-2-C^{14}$, it might he considered that methionine in the rice diet seems to be limited. However, it seems to be not 주 mostly limited. 3. And due to the results of radioactivity distribution in the organs by time being, the radioactivity in the liver tissue showed appearently higher readings at this methionine study compared with the results at the lysine study shown by HAW and his co-worker. This might be interpreted, though it is not clear, that liver might require methionine as a deficient amino acid at the tissue because methionine is limited at the rice diet.

• Applied Microscopy
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• v.21 no.1
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• pp.27-45
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• 1991

To study the effects of dietry methionine level on lipid peroxidation of rats, rats were fed vitamin E, selenium and methionine - deficient diet or the same diet supplemented with various levels(0.3%, 0.6%, 0.9%) of methionine for 6 weeks. The biochemincal and mophological changes in the rat liver were investigated. Lipid peroxide levels in plasma and hepatic mitochondrial fraction of MF rats were more increased than those of control rats. However, supplementation with 0.6% methionine modified this increment. Catalase activity was decreased in hepatic mitochondrial fraction from rats fed MF diet. Methionine supplementation did not induce this enzyme. The ultrastructural evidence for lipid peroxidation was found in plasma membranes facing sinusoids. The most striking changes in including disruption and loss of microvilli and development of numerous lipid droplets occurred in rats fed MF diet. These changes were not effectively prevented by the same diet supplemented with 0.3% or 0.9% methionine, but supplementation with 0.6% methionine modulated more or less the changes.

• The Korean Journal of Pharmacology
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• v.7 no.1
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• pp.37-52
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• 1971

It has been demonstrated repeatedly that the administration of ethionine, a methionine analogue, will produce destruction of pancreatic tissue and liver cells. Destruction of acinar cells of pancreas by the administration of excess methionine similar to that seen after feeding diets supplemented with ethionine was also reported, but the liver was not involved by this amino acid. In an attempt to reproduce the results of these investigators the tissue damages were found to be slight and seen only irregulary in rats receiving ethionine on a complete diet and the result of tissue damages by excess methionine were also controversial. The present studies describe the toxicity of dietary supplemented ethionine and methionine to liver and pancreas in rats fed a low protein diet. Hundred five albino rats weighing around 120 gm were divided into three groups as follows; 1) Control group: A low protein diet containing 8% casein was fed throughout the experimental period. 2) Methionine group: A low protein diet (7% casein) supplemented with 1% methionine was used. 3) Ethionine group: A similar diet as methionine group except the supplementation of 1% ethionine instead of methionine was used. Five animals per wee from each group were killed for 6 weeks. The liver and pancreas were fixed in 10% formalin and histologic sections were prepared and stained with hematoxylin eosin. Serum amylase was expressed as much of glucose liberated from a starch substrate. The glucose was determined by the method of Nelson (1944). Serum glutamic pyruvic transaminase (SGPT) and glutamic oxaloacetic transaminase (SGOT) were determined by the technique described in Sigma Bulletin.

• Journal of Nutrition and Health
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• v.19 no.4
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• pp.224-232
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• 1986

This study was performed to investigate the effects of different kinds of dietary protein [plant protein ; Isolated Soy Protein(ISP), animal protein ; casein] on protein and lipid metabolism in rats fed with coffee and/or methionine in diet during four weeks of growing period after weanling. Forty male growing rats fo Sprague-Dawley strain, weighing 92.5$\pm$1.8g, were distributed into 8 groups by randomized complete block design, and fed diets containing 15% of protein by weight either as ISP or casein and 10% ofcalories as corn oil, supplemented with coffee and /or methionine for 4 weeks. Coffee were added at a concentration of 1.4% of diet as instant coffee, and methionine were added to ISP or casein diet to be 0.6% of diet as DL-methionine. Results were followed ; Body weight gain, F.E. R and P.E.R tanded to be higher in methionine added groups than non-methionine groups. The nitrogen content of feces was significantly higher in coffee groups than non-coffee groups, and tended to be higher in ISP groups than casein groups. but was not significantly different with or without methionine. thus, apparent protein digestilbity was significantly lower in coffee groups than non-coffee groups and was significantly lower in ISP groups than cesein groups, but was not significantly different with or without methionine. Total cholesterol content of serum tended to be higher in coffee groups than non-coffee groups, and tended to be lower in methionine groups than non-methionine groups.

• Korean Journal of Poultry Science
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• v.44 no.2
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• pp.67-73
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• 2017

The purpose of our study was to determine the effects of varying levels of energy, protein, and amino acids on the performances of laying hens. A total of 240 Hy-Line Brown laying hens at 36 weeks of age were used in this 4-week feeding trial. The hens were randomly allocated to five treatment diets, with eight replications of six hens in each replicate cage. The treatment diets were as follows: A- basal diet + 18% crude protein, metabolizable energy 2,800 kcal, total (methionine + cysteine) 0.65%; B- basal diet + 17% crude protein, metabolizable energy 2,700 kcal, total (methionine + cysteine) 0.59%; C- basal diet + 16.5% crude protein, metabolizable energy 2,700 kcal, total (methionine + cysteine) 0.59%; D- basal diet + 16.5% crude protein, metabolizable energy 2,700 kcal, total (methionine + cysteine) 0.54%; and E- basal diet + 16% crude protein, metabolizable energy 2,680 kcal, total (methionine + cysteine) 0.54%. The study results revealed that the hen-day egg production of hens that were fed with low-energy diets (B, C, and D) was comparable with that of hens fed with high-energy diet A, whereas average daily feed intake in hens fed treatment diet D and E was significantly higher (P<0.05) than that in hens fed treatment diet A. Overall, the eggshell thickness was unaffected by any of the treatment diets. Egg weight was comparable among the treatment diets, except for treatment diet E. Haugh unit improved with decreasing levels of dietary energy, protein, and methionine + cysteine in the diet. We can summarize that laying hens fed with low dietary energy and low crude protein treatment diets B, C, and D had satisfactory performance compared with those fed with high-energy treatment diet A. This indicates that there is the potential to reduce feed costs by formulating diets with lower energy and low protein levels.

• Journal of the Korean Chemical Society
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• v.10 no.3
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• pp.109-113
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• 1966

By the micro-bioassay method, quantitative studies were made on the increment of body weight and changes in free-lysine and threonine contents in the liver and plasm of rats, when they were fed on sucrose diet added with 0.3% DL-methionine, 0.36% DL-threonine and 0.1% L-tryptophane. The results of the experiment were as follows: (1) The weight gain and growth gain of rats decreased more when they were fed on 0.36% DL-threonine diet (Sth-group) than 0.3% DL-methionine diet (Sm-group). But those gain were increased when they were fed on 0.1% L-tryptophane diet (Str-group). (2) The contents of free-lysine and threonine in plasm and liver of rats were decreased more when they were fed on 0. 36% DL-threonine diet than 0.3% DL-methionine diet. And, those free amino acids were increased greatly when they were fed on 0.1% L-tryptophane diet. (3) The increment of body weight was the biggest when the free amino acid pattern showed that the contents of free-lysine decreased and threonine increased in the plasm and liver of rats being fed on 0.l% L-tryptophane diet.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.7
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• pp.1090-1095
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• 1999

In order to minimize the occurrence of fatty liver, this study investigated how adding a high level of dietary choline or methionine affected the lipid metabolism of Tsaiya ducks. Feeding trials were conducted with sixty Tsaiya ducks during their growing period, when they were 8-12 weeks old, and during their laying period, 10-14 weeks after the onset of laying when they were 26-30 weeks old. The ducks were randomly assigned to one of three groups: a control group (basal diet), a group in which methionine was added to the basal diet, and a group in which choline was added to the basal diet. The levels of methionine and choline added were twice as high as the levels recommended by the NRC for layer-type chickens. Experimental results indicated that adding choline to the basal diet of growing ducks significantly (p<0.05) increased their body weight, while adding methionine significantly (p<0.05)reduced their body weight. Adding either choline or methionine reduced the ducks' liver fat content in both the growing and the laying periods (p<0.05). Ducks receiving added methionine or choline in their diets displayed enhanced egg production (p<0.05). Adding choline increased serum triacylglycerol (TG) in the laying period (p<0.05). Adding either choline or methionine did not significantly (p>0.05) affect the t-globulin level. Adding methionine increased the activity of liver malic dehydrogenase in both the growing and the laying periods, and increased fatty acid synthetase in the laying period (p<0.05). While adding choline markedly (p>0.05) increased VLDL and apo B in both the growing and the laying periods, it decreased HDL and apo A in the laying period (p<0.05). This study found that adding a high level of choline to the basal diets of Tsaiya ducks in both the growing and the laying periods had beneficial effects. Furthermore, adding methionine affected the lipid metabolism of Tsaiya ducks to a lesser extent than adding choline.

• Korean Journal of Agricultural Science
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• v.34 no.2
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• pp.135-142
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• 2007

Holstein cows(n=8) were assigned to control diet(n=4) and treatment diet(n=4) containing products of Bromelain(50g/kg) and Zn-methionine (133g/kg). Basal diet was mixed as total mixed rations with 60% concentrate and 40% roughage(rice straw) and fed for 8 weeks. The milk production, somatic cell counts in milk were measured and determined. The results were summarized as follow. Average milk production was higher for cows fed treatment diet(30.2kg/d) than cows fed control diet(29.6kg/d) (P<0.05). The somatic cell counts was significantly lower for cows fed treatment diet ($179.8{\times}10^3/ml$) than cows fed control diet ($260.8{\times}10^3/ml$)(P<0.05). In conclusion, supplementation of both Bromelain and Zn-methionine increased milk production and reduced somatic cell counts in milk.

• Journal of the Korean Society of Food Science and Nutrition
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• v.17 no.4
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• pp.376-383
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• 1988

The effect of dietary methionine level on lipid peroxidation of rats was studied. Rats were fed vitamin E- selenium- deficient diet or diet supplemented with various levels (0.3, 0.6, 0.9%) of methionine. In rat fed MF diet, body weight gain and feed efficiency ratio were decreased compared with those of control rats, but reversed by supplementation with 0.3 and 0.6% methionine. Lipid peroxide levels in plasma and hepatic mitochondrial fraction of MF group rats were significantly higher than those of control rats. However, supplementation with 0.6% methionine modified this increment. GSH-Px activity was decrased to varying degrees in erythrocyte and hepatic mitochondrial fraction from rats fed MF diet. Methionine supplementation did not affect induction of this enzyme activity. Examination of hepatocytes by electronmicroscopy showed that Influence of vitamin E, selenium, and methionine deficiency was mainly characterized by lipid droplets, swollen mitochondria and microvilli destruction. Supplementation with various levels of dietary methionine modified these changes to some extent. The results of this experiment indicated that MF diet causes significant change in lipid peroxide level, GSH-Px activity and morphology of rats which these changes may lessen by supplementation with 0.6% methionine.