• Environmental Analysis Health and Toxicology
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• v.1 no.1
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• pp.55-60
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• 1986

The writer measured methemoglobin formation with solvent (n-hexane, ethylacetate and methanol) organic extracts from the sediments of the Wonju stream run through Wonju city on Octover 2nd, 1985. Hemoglobin was converted to methemoglobin with the n-hexane extract obtained from the sediment of the St. C and St. D was polluted industrial wastewater, but not with that from the St. B was polluted with the urban wastewater. The formation of methemoglobin was remarkable with the all solvent extracts from the sediment of the uper stream of the Wonju stream. Doses of the solvent extracts for 20% methemoglobin formation in the researched Wonju streams were as follows; 0.095～0.28 mg/m.$\ell$ in n-hexane extract. 0.85-1.3 mg/ m$\ell$ in methanol extract and 1.95～2.80 mg/m$\ell$ in ethylacetate extract.

• Environmental Analysis Health and Toxicology
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• v.7 no.1_2
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• pp.35-43
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• 1992

Mugwort has been used as a Korean folk medicine in treating liver diseases acting as an analgesics, sedative, diuresis, choleretics. This study was perfomed to evaluate the effect of mugwort extracts on the changes of enzyme activities, lipid accumulation of the serum and liver, when hepatotoxicity was induced by benzo(a)pyrene. The results are as follows: 1. Mugwort water extract administration prevented the increase of serum and liver AST, ALT, LDH, ${\gamma}$-GTP, liver ALP activities and bilirubin content caused by B(a)P injection. 2. The increase of serum and liver ALT, LDH, ${\gamma}$-GTP, serum AST activities and liver bilirubin contents in B(a)P treated group were decreased by mugwort methanol extract treatment. 3. Serum and liver total cholesterol, phospholipid, triglyceride level and serum HDL-cholesterol level were increased by B(a)P treatment. After combined treatment of mugwort water and methanul extracts, these lipid content were significantly decreased. 4. The hepatotropic effect of mugwort water extract and after-treatment against B(a)P induced hepatotoxicity was superior to that of methanol extract and pretreatment.

• Microbiology and Biotechnology Letters
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• v.25 no.2
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• pp.178-182
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• 1997

This study was carried out particutarly focusing on the antitumor effects of extracts obtained from mushroom, Daedalea dickinsii against Hela cell, Hep $G_{2}$, cell, L 929 cell and Sarcoma-180. Antitumor effect of hot water extracts obtained from Daedalea dickinsii against Hela cell showed at the highest level of 70% when adiministrated at the concentration of 1.5 mg/100 ml, however Hep $G_{2}$, cell inhibited 40% at the same concentration of hot water extracts. Antitumor effects of methanol extract of Daedalea dickinsii against Hela cell indicated at the highest level of 60% when 4 mg/ml was administerated. Antitumor effect of hot water extract of Daedalea dickinsii inhibited 51.03% against L929 cell. The solid tumor sarcoma-180 growth inhibition of methanol extract of Deadalea dickinsii inhibited 45.67% when administrated at the concentration of 60mg/kg, and the life prolongation was higher 30.88% than that control group.

• The Korea Journal of Herbology
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• v.21 no.2
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• pp.55-62
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• 2006

Objectives : This study was carried out to investigate the biological activities of methanol extracts of fermented Camellia japonica leaf and flower. Methods : Methanol extracts of fermented Camellia japonica leaf and flower were prepared and a dose of 100 and 400mg/kg/day was administered orally into mice. And after appropriate weeks, changes of serum enzyme activities were investigated to confirm its effects on serum glucose, cholesterol and short term administration safety. Results : Fermented flower extract showed significant decrease of serum level of cholesterol. And showed no toxicity on kidney and liver within the dose of 400mg/kg/day. Conclusion : Thus above result showed no toxicity on kidney and liver in male and female mice.

• Biomolecules & Therapeutics
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• v.9 no.4
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• pp.244-248
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• 2001

This study was performed to investigate therapeutic effects of Carthami Semen, Paeoniae Raidx extracts and chitosan on the growth and differentiation of human periodontal ligament cell. We found that co-treatment of methanol extracts of Carthami Semen and chitosan significantly increased the growth of human periodontal ligament cell. However, the sigle treatment groups of the extracts showed only 20-30% of the growth increase. Alkaline phosphase activity, one of differentiation markers, was increased approximately 1.5- fold by co-treatment of methanol extract of Carthami Semen and chitosan and calcified nodule formation was also increased at the similar levels as the alkaline phosphatase. But the single treatment groups showed only 20-30% increases. These results suggest that Carthami Semen and chitosan co-treatment can be used efficiently for periodontium regeneration.

• Korean journal of food and cookery science
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• v.27 no.5
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• pp.523-530
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• 2011

This study was conducted to investigate antimicrobial, antioxidant and anticancer activities of Opuntia humifusa (OH) petal extracts. The methanol and hexane extracts of OH petals showed their highest antimicrobial activity against Clostridium perfringens. The OH petal butanol fraction had the best antioxidative peroxynitrite scavenging activity among OH petal extracts. The DPPH scavenging activity of OH petals was lower than the peroxynitrite scavenging effect. The hexane and methanol fractions at a concentration of 200 ${\mu}g$/mL inhibited proliferation >80% in four kinds of human cervical cancer cells(B16F10, HepG2, HT-29 and MCF-7). In particular, the anticancer effect against B16F10 human skin cancer cells at the same concentration was higher than that in the other cancer cells.

• Food Science and Preservation
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• v.17 no.1
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• pp.107-116
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• 2010

We prepared extracts from Ulmus davidiana (root, Korean source; URK) and Ulmus davidiana (bark, Korean source; UBK). URK extracts obtained with all tested solvents showed the highest antioxidant effects on fish oils. Both treatments containing 0.1% (v/v) extract from URK and UBK each showed that peroxide values of 30 meq/kg were maintained for 6 h and levels of 40 meq/kg were apparent for up to 18 h, indicating that antioxidative activity seemed to sustain during all tested time periods. Compared with commercial antioxidants, butanol and methanol extracts diluted to 0.05% (v/v) had similar antioxidative effects. Water and butanol UBK extracts diluted to 0.1% (v/v) both showed the highest antioxidative activities. After addition of metal ions, methanol and butanol URK extracts diluted to 0.1% (v/v) showed enhanced antioxidative activity. UBK ethanol extracts displayed superior antioxidative activity and a constant peroxide value throughout storage. However, in the case of Perilla oil, $\alpha$-tocopherol which is known as a natural antioxidant did not show any antioxidative activity except in the BHT. Methanol and butanol URK extracts diluted to 0.2% (v/v) showed superior antioxidative activities throughout the experiment. A methanolic UBK extract (0.2%, v/v) also had a similarly increased antioxidative effect. In tests involving addition of metal ions to all extracts, the methanolic UBK extract (0.2%, v/v) showed excellent antioxidative activity. When lard was tested, antioxidant levels did not differ significantly among extracts prepared using four different solvents at either 0.05% or 0.1% concentrations (both v/v). Addition of metal ions at levels of 0.05% or 0.1% (w/v) to these extracts had no significant additive effect on oxidation.

• Journal of Nutrition and Health
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• v.31 no.5
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• pp.921-926
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• 1998

The purpose of this study was to investigate the potential hypolipidemic effect of adlay extract in streptozotocin-induced diabetic rats. Fifty six rats were fed either a control diet or adlay extract diets of : Methanol 1%(Ml%) ; Methanol 2%(M2%) ; Methanol 4%(M4%) ; Chloroform-Methanol 1%(CMl%) ; Chloroform-Methanol 2%(CM2%) ; Chloroform-Methanol 4%(CM4%) for 3 weeks. The amount of extracts added was 1%, 2% or 4% by diet weight respectively. The levels of glucose, total cholesterol(TC), high-density-lipoprotein cholesterol (HDL-C), free-fatty acid(FFA) and triglyceride(TG) in plasma, liver and skeletal muscle were compared. Among diabetic rats, there were no significant differences in the plasma level of glucose and TC regardless of a different extraction procedure or different amount of extracts added. While the plasma 7G level tended to increase with times passed in diabetic control group, was not increased with times passed in CM groups and was significantly lower in CM groups at 3rd week. Compared to the diabetic control group, the levels of FFA tended to be lower in all M groups and were not different in all CM groups. The levels of HDL-cholesterol were not different in all M groups and were significantly lower in all CM groups than diabetic control group. Compared to diabetic control group, liver triglyceride level was lower in M4% group and no significant difference was seen in Ml%, M2% and all CM groups. Muscle triglyceride level tended to be lower in Ml%, M2% and CM2% group and significantly was lower in M4% and CM4% group. Thus, it can be suggested that a CM extract of adllay could have a potential hypotriglyceridemic effect on diabetic subjects. (Korean J Nutrition 31(5) : 921∼926, 1998)

• Journal of Life Science
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• v.18 no.10
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• pp.1415-1419
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• 2008

This study was carried out to determine the inhibitory effects of methanol extracts from Korean Orysa sartiva and Coix lachryma-jobi var. ma-yuen on mutagenicity using Ames test and growth of AGS human gastric adenocarcinoma and HT-29 human colon cancer cells. Both treatments of methanol extracts (5 mg/assay) from Orysa sartiva and Coix lachryma-jobi var. ma-yuen to Ames test system inhibited aflatoxin $B_1$ ($AFB_1$) induced mutagenicity by 76%. In case of N-methyl-N'-nitro-N-nitrosoguamidine (MNNG) induced mutagenicity, the methanol extracts (5 mg/assay) from Orysa sartiva and Coix lachryma-jobi var. ma-yuen showed 79% and 69% inhibitory rate, respectively and the inhibitory effect was a little stronger in Orysa sartiva Inhibitory effects of methanol extracts from Orysa sartiva. and Coix lachryma-jobi var. ma-yuen on the growth of AGS and HT-29 human cancer cells were increased as dose dependent patterns and the inhibitory effects on AGS and HT-29 cells were similar. The above results indicate that the consumption of these cereals, which contain many nutrients with good quality, may be recommended as potent functional foods for improving health.

• The Korean Journal of Food And Nutrition
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• v.22 no.4
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• pp.625-632
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• 2009

This study determined the effects of added buckwheat extract on the rate of corn starch hydrolysis in vitro as well as blood glucose responses through its supplementation in healthy subjects. The rate of corn starch hydrolysis in the presence or absence of various buckwheat extracts was determined in an in vitro enzyme/dialysis system for 2 hr. The buckwheat was extracted by water, ethanol(40%, 70%, 100%) and methanol(40%, 70%, 100%), respectively. Twenty percent(w/w) additions of the ethanol, methanol and water buckwheat extract to corn starch solution significantly reduced the starch hydrolysis at every minute for 2 hr(p<0.05). The calculated hydrolysis indices of the buckwheat extracts were in the order of 100% ethanol extract(50), 100% methanol(54), 40% ethanol(58), 40% methanol(62), 70% methanol(64), 70% ethanol(68), water (82). For the blood glucose response study, groups of 12 volunteers were given 50 g of boiled rice with or without buckwheat extract(10% and 20% of starch weight) using the 100%, 70%, and 40% ethanol extracts, respectively. The addition of each buckwheat ethanol extract significantly reduced blood glucose concentrations at three or more points during 2 hr and also reduced the mean peak rise and area under the blood glucose curve(p<0.05). The calculated glycemic index(GI) values for all ethanol buckwheat extract groups were significantly decreased compared to the control(rice). At the concentrations of 20%, the buckwheat 100% ethanol extracts lowered the GI by 68%. The 100% ethanol extract was more effective than the 70% and 40% extracts for reducing GI. Therefore, the 100% ethanol buckwheat extract would be the most therapeutically useful in modifying postprandial hyperglycemia.