• Maxillofacial Plastic and Reconstructive Surgery
• /
• 제21권1호
• /
• pp.23-28
• /
• 1999

ALP (alkaline phosphatase) is a membrane-bound metalloenzyme that is expressed in osteoblasts, hepatocytes, lung, kidney, endothelial cells, leukocytes and other cells. Normal soft tissue and skin show little tissue nonspecific ALP (TN-AP), However, scar tissue contains high levels of TN-AP activity, and in fact, TN-AP is expressed intensely in regenerating connective tissue after the wounding. The purpose of this study was to evaluate the change of ALP expression in hypertrophic scar model in rabbits and the effect of triamcinonolone on ALP expression. Adult male New Zealand white rabbits, weighing about 2.5 kg, were used. After full-thickeness wounding over the ventral surface of each ear, either saline (control ear) or triamcinolone (contralateral ear) was injected on day 16. Rabbits were sacrificed on day 3, 7, 15, 17, 19, 23, and the specimens were retrieved en bloc. Histologic and immunohistochemical examinations of tissue samples were done. The results obtained were as follows: On day 3, ALP reaction was observed on fibroblasts and inflammatory cells in wound margin. On day 7, ALP reaction was more intense than day p in capillaries, inflammtory cells, and fibroblasts behind newly formed epithelium. On day 15, ALP reaction was lessened in both groups and appeared mainly in subepidermal capillary network, Since day 17, ALP reaction was lessened in both groups and weaker in triamcinolone-injected group than in saline-injected group. These results suggest that ALP reaction isn't increased in triamcinolone-injected scar and triamcinolone reduces scar not by increasing TN-AP expression but other mechanism.

• 생명과학회지
• /
• 제24권2호
• /
• pp.186-190
• /
• 2014

본 논문에서는 삼세기(Shaggy sea raven, Hemitripterus villosus)를 실험 재료로 선택하여 Carbonic Anhydrase Isozymes (CAs) 중의 하나인 CA III에 대한 연구를 SDS-PAGE, Isoelectric Focusing (IEF), Western blot analysis의 방법을 통하여 진행하였다. SDS-PAGE와 Western blot 결과 삼세기 아가미, 혈액, 장, 간, 신당, 근육, 심장조직에서 CA III의 분자량인 30 kDa의 band가 확인되었다. 삼세기의 근육과 아가미에 대한 등전점 전기영동(IEF)과 Western blot analysis 결과 pI 7.0 부근에서 band가 형성되는 것을 확인할 수 있었다. 특히 SDS-PAGE와 IEF 실험결과 삼세기의 아가미 조직에서 CA III의 발현량이 다른 조직들에 비하여 우세하게 나타났다. 이는 아가미가 다른 조직들과 달리 어류의 생체기관 중 유일하게 외부와 직접 접촉이 가능한 조직으로서 활성산소에 대한 손상을 최소화하기 위한 것으로 사료된다.

• 한국미생물·생명공학회지
• /
• 제20권3호
• /
• pp.295-301
• /
• 1992

토양으로부터 alkaline protease 생성능이 강한 Streptomyces griseus HC-1141을 분리하였으며, 정제 효소의 최적작용 pH 와 온도는 8.0, 60'C 였으며, pH 7.0-9.0의 범위와 $60^{\circ}C$이하에서 안정하였다. 금속 이온중 $Mn^{2+}$, $Ca^{2+}$, 등에 의해 활성이 증대되었으나 $Hg^{2+}$, $Cu^{2+}$, $Zn^{2+}$, $Ba^{2+}$, $Fe^{2+}$ 등에 의해 효소 활성이 저해되었고, 효소활성 저해제 중 Epsilon-aminocaproic acid, 2,4-dinitrophenol, iodine 등에 의해서는 현전한 효소활성저해가 관찰되지 않았으나 ethylenediaminetetraacetic acid와 p-chloromercuribenzoic acid에 의해 활성저해가 관찰되어 효소분자 중 SH기가 활성에 어느정도 관여하는 metallo enzyme으로 추정되었다. 정제효소의 $K_m$, $V_{max}$ 및 활성화에너지는 $2.229{\times}10^{-4}$M, $46.08 {\mu}$g/min, 3.643 kcal/mol 이었으며 hemoglobin과 egg albumin보다 casein을 더 잘 가수분해하였다. 또한 여러 가지 detergent에 대하여 강한 저항성을 가지고 있었다.

• Journal of Nutrition and Health
• /
• 제5권2호
• /
• pp.91-103
• /
• 1972

Zinc is one of the essential trace elements in the living organism for growth and health. The first identified metalloenzyme, carbonic anhydrase, is a zinc compound and several others have been described since. Among zinc deficiency syndromes in animals porcine parakeratosis has been successfully treated with zinc supplements, and in man a syndrome of anemia, hypogonadism, hepatosplenomegaly, and dwarfism, prevalent in parts of Iran and Egypt, has been ascribed to lack of zinc in the diet. Dietary zinc excess in the rat is manifested by a hypochromic, microcytic anemia, poor growth, reduction in liver catalase and cytochrome oxidase. The present study is an attempt to delineate the changes of tissue contents of trace elements, especially of iron, copper and zinc in liver and kidneys of the rats. Weanling albino rats, weighing 60 to 80gm. were used in this experiments. The rats were housed in cages with aluminum floors and received feed and distilled water ad libitum. Animals were divided into three groups, control, low zinc diet and high zinc diet groups. The high zinc diet group was subdivided into 0.5% Zn and 0.7%Zn groups. The supplementary copper or iron was added to the high dietary zinc groups. The animals were sacrificed and the tissues were washed several times with deionized water. The wet digested samples were analyzed by Hitachi Model 207 atomic absorption spectro-photometer for the determination of iron, copper and zinc in the liver and kidney. Hemoglobin level in the blood was measured by cyanmethemoglobin method. The results of this study are as follows: 1) All rats fed high zinc diets and low zinc diets gained less weight than control. Weight gain was not improved by the supplementary copper or iron and both. 2) Hemoglobin concentration was decreased significantly in the rats fed high zinc diets and less in the low zinc diet. Supplementary copper and iron to the higher zinc diet appeared to give some improvement of anemia. 3) The iron contents of the liver and kidneys were significantly decreased in the high zinc groups and the reduction was more significantly in the rats receiving higher zinc diet (0.7%). The supplementary copper caused a further depression of liver iron. On the other hand, the iron, added to the high zinc diet lessoned the severity of the decrease in liver iron and caused kidney iron to be maintained almost at the level found in the rats fed by zinc and supplementary copper diet. 4) High zinc diets did not change copper content of the liver and kidney. Supplementary copper elevated the concentration in the liver and kidney and added iron had no effect on the accumulation of copper in the liver and kidneys. 5) The high zinc diets caused marked increases of zinc content in the liver and kidney. Supplementary iron to the high zinc diet caused increases of zinc contents of liver and kidneys.

• 생명과학회지
• /
• 제24권12호
• /
• pp.1371-1377
• /
• 2014

활성산소종(ROS)은 환경 스트레스 및 병원체의 침입에 의한 산화 대사의 자연적인 산물로써 생물에서 생산된다. 산화적 스트레스에 의해 생성되는 superoxide 음이온 및 과산화수소와 같은 ROS는 세포와 조직에 독성을 나타낼 수 있고, 이 과정에 관여하는 superoxide dismutase (SOD)는 중요한 metalloenzyme이다. 최근 연구는 올리브 넙치(Paralichthys olivaceus)에서 SOD의 부분 유전자가 benzo[a]pyrene에 의해 강하게 발현이 유도되고 산화 스트레스 반응의 지표라고 확인하였지만, 바이러스성 감염에 대한 전사적 반응에 대해서는 조사되지 않았다. 본 연구에서는 항바이러스 반응에서 넙치 SOD의 기능을 알아보기 위해 공간 및 시간적 발현 프로파일을 분석하였다. 넙치 SOD 전사체는 정도의 차이는 있지만 다양한 기관에서 보편적으로 발현되었으며, 근육, 간, 뇌에서는 높게 발현되었고, 위와 비장에서는 상대적으로 낮게 발현되었다. VHSV 감염 후 넙치 콩팥에서 SOD 발현은 3시간 이내에 증가하였으며 점차적으로 감소하여 감염 2일째 원래 수준으로 돌아갔다. 검사 조직에 따라 발현이 유도되는 시간의 차이는 있지만 근육, 간, 뇌에서도 콩팥과 유사한 발현양상을 보였으며, 공통적으로 급성적 면역반응에서는 발현이 증가하지만 만성적 면역반응에서는 감소하였다. 이상의 결과들을 종합해 볼 때, 넙치 SOD는 넙치(P. olivaceus)의 면역 방어 시스템에 중요한 역할을 하고 넙치의 산화 스트레스에 대한 보호 효과에 기여할 것으로 기대할 수 있다.