• Title/Summary/Keyword: Metal-chelating

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A Study on the Physicochemical Properties and the Nitrite Scavenging Ability of Japanese Sumac Extracts (붉나무 추출물의 이화학적 특성과 아질산염 소거능에 관한 연구)

  • Noh, Jeong-Sook;Jeong, Kap-Seop
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.2
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    • pp.527-534
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    • 2019
  • Japanese sumac (Rhus javanica) is one of the common herbaceous plants growing over the country. This study was conducted to investigate the extraction characteristics and physiological activities inluding nitrite scavenging ability of the water extracts from leaf, fruit and bark of Japanease sumac. Extraction yield was 6.62~13.84%, free amino acids were detected as 24 kinds with 37.9 mg/100g in leaf extract, 23 kinds with 27.0 mg/100g in fruit extract and 27 kinds with 39.0 mg/100g in bark extract, respectively, and seven kinds essential amino acids were detected. Total contents of flavonoids equivalent to naringin were 587.2 mg/100g in bark extract, 557.3 mg/100g in fruit extract and 379.9 mg/100g in leaf extract, respectively. Total contents of phenolics equivalent to gallic acid were 111.2 mg/100g in leaf extract, 108.4 mg/100g in fruit extract and 80.4 mg/100g in bark extract, respectively. The nitrite scavenging ability of extracts was order of 61.93% in bark extract>57.38% in fruit extract>55.49% in leaf extract, and was 78.1~100% of those of BHT (butylated hydroxytoluene) equivalents at pH 1.2. The electron donating ability was order of 47.38% in fruit extract>43.06% in leaf extract>38.55% in bark extract, and was compared to 65.6%, 58.8% and 53.6% of those of BHT equivalents, respectively. The reduction power was evaluated to 37% higher in leaf extract, 43% higher in fruit extract and 46% higher in bark extract than those of BHT equivalents. The metal chelating ability of extracts was considerably low and was order of 27.3% in bark extract>20.6% in leaf extract>11.2% in fruit extract.

In Vitro Antioxidant Activity of Alnus firma Extracts (사방오리(Alnus firma) 추출물의 in vitro 항산화 활성)

  • Choi, Hye Jung;Joo, Woo Hong
    • Journal of Life Science
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    • v.29 no.2
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    • pp.231-238
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    • 2019
  • This study evaluated the antioxidant activity of the extract and fractions of Alnus firma. Alnus firma had the highest total phenolic content ($452.80{\pm}7.01{\mu}g$ gallic acid equivalents/mg) in a methanol (MeOH) fraction and the highest total flavonoid content ($112.29{\pm}11.14{\mu}g$ rutin equivalents/mg) and antioxidant capacity ($936.23{\pm}0.07{\mu}g$ ${\alpha}$-tocopherol equivalents/mg) in an ethylacetate (EA) fraction. The antioxidant activities of various solvent extract fractions of Alnus firma were evaluated using various antioxidant assays, including ${\beta}$-carotene-linoleate assay, reducing power assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, metal chelating activity assay, superoxide anion radical scavenging assay, and lipid peroxidation inhibition assay using the ferric thiocyanate method. These activities were compared with those of ascorbic acid, butylated hydroxyanisole (BHA), gallic acid (GA), butylated hydroxytoluene (BHT), and ${\alpha}$-tocopherol. First, at a $250{\mu}g/ml$ concentration, the EA and MeOH fractions of A. firma showed 92.43% and 89.20% DPPH radical scavenging activity, respectively. Second, $50{\mu}g/ml$ of the EA fraction exhibited 72.49% superoxide anion radical scavenging activity, a little greater than the same dose of GA (60.88%). Finally, 0.5 and 1 mg/ml of the EA fraction showed 73.45% and 73.29% inhibition of peroxidation in the ${\beta}$-carotene-linoleic acid system, respectively. The decreasing order of reducing power was EA fraction > n-butanol (BuOH) fraction > dichloromethane (DCM) fraction > n-hexane (HX) fraction. The results obtained in the present study indicated that Alnus firma can be used as an easily accessible potential source of natural antioxidants.

Comparison of antioxidant activities of pearled and wholegrain barley harvested in Jeju (도정 유무에 따른 제주산 보리의 항산화 활성 비교)

  • Kang, Yuri;Kim, Hyun Jung
    • Korean Journal of Food Science and Technology
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    • v.53 no.3
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    • pp.252-259
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    • 2021
  • Barley contains health-beneficial compounds, such as phenolic acids, flavonoids, lignans, and tocopherols. The antioxidant activities of pearled and wholegrain hull-less, premature, and black barley harvested in Jeju were investigated by measuring total phenolic content (TPC), total flavonoid content (TFC), phenolic compounds, tocopherols (T), and tocotrienols (T3), along with their in vitro antioxidant activities. Consequently, TPC and TFC in wholegrain barley groups were higher than those in pearled barley. Gallic acid (1.55-2.98 ㎍/g) and protocatechuic acid (0.67-2.84 ㎍/g) were the predominant phenolic compounds in barley. Total T and T3 concentrations of wholegrain barley groups were significantly higher than those of pearled barley (p<0.05). Except for the metal chelating effect and reducing power, the in vitro antioxidant activities of wholegrain barley were significantly higher than those of pearled barley. These results indicate that wholegrain Jeju barley can be used as a natural antioxidant source in the food industry.

A Study on the Evaluation of the Adsorption Efficiency of Heavy Metals by the Content of Jellyfish Extract at Immunity Reaction in Alginate bead (알긴산 비드에 혼합된 해파리 면역 반응물질 함량에 따른 중금속 흡착효율 평가에 관한 연구)

  • Jong Hwan Kim;Hyeok Jin Park;Inho Choi;Eunjin Kim;I Song Choi;Jong-Min Oh
    • Journal of Environmental Impact Assessment
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    • v.32 no.6
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    • pp.431-436
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    • 2023
  • As the industry develops, the amount of heavy metals flowing into the ecosystem is increasing. Heavy metals are difficult to decompose and remain in the ecosystem for a long time and cause toxicity, which is removed by physicochemical methods such as adsorption, filtration, and chemical precipitation during water treatment. In this study, Alginate bead was selected as a chelating resin for adsorbing and removing heavy metals, and the Jellyfish Extract at Immunity reaction (JEI) were mixed to evaluate the adsorption efficiency of heavy metals accordingly. beads mixed with JEI showed high adsorption efficiency in lead (79-99%) and copper (64-70%) according to the characteristics of Alginate, and low adsorption efficiency in cadmium (25-37%) and zinc (5-6%). Although heavy metal adsorption did not increase in proportion to the content of JEI, 50% and 100% JEI beads showed significant increases. As a result of applying the reaction rate equation, it was found that it was more suitable for the pseudo-secondary reaction equation than the pseudo-first reaction equation.