• Title/Summary/Keyword: Metabarcording

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Analysis of the characteristics of the environment and fish community in the Gwanggyo Lake Park area using the environmental DNA technique (환경 DNA 기법을 활용한 광교호수공원 일대의 시기 및 수환경 특성별 어류상 분석)

  • Won, Su-Yeon;Kang, Yu-Jin;Song, Young-Keun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.25 no.5
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    • pp.77-88
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    • 2022
  • This study aims to understand the relationship between the distribution of fish species in the two water ecosystems and the habitat factors according to the survey period targeting Gwanggyo Lake Park in the city. There are studies on the appearance and distribution of species by applying eDNA to freshwater ecosystems. However, in the domestic, streams are the target, and studies on the relationship between species distribution and habitat environment in two water environments are lacking. We conducted to analyze the species list and relationship with habitat factors using eDNA research in May and October at 21 points in Gwanggyo Lake Park, Suwon City, which were connected to lakes and streams. As a result, there was no species difference in the water environment according to the survey period. However, the total number of reads during the spawning season(May) was 3,126,482, which was more than double that after the spawning season(October). Tolerant species appeared in Woncheon Lake with a slow or stagnant flow, but there was no significant correlation between species and habitat factors depending on the survey period. On the other hand, intermediate and sensitive species appeared in the Woncheon stream with high flow. There was a significant correlation between the low temperature during the spawning season and the high dissolved oxygen content after the spawning season(P<0.001, Tem.: 20.7±2.6℃, DO: 8.6±1.7). It is expected that environmental DNA will be used to survey species and suggest monitoring methods according to the survey period.

Changes in the Biofloc Bacterial Community Caused by Planting of Triglochin maritimum (지채(Triglochin maritimum)입식에 따른 바이오플락 사육수 내 세균 군집의 변화)

  • Jea-Hwang Cho;Su-Kyoung Kim;Hyeon-Ho Lim;Dea-Hee Kim;Hyon-Sob Han
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.1
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    • pp.99-108
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    • 2023
  • In this study, changes in biofloc-related and pathogenic bacteria in both low and high concentration biofloc breeding water planted with the halophyte (Triglochin maritimum were investigated). In the low-concentration biofloc breeding water, the ratio of bacteria related to the nitrogen cycle was initially 59.57% and, it decreased to 26.57% at the end of the experiment while other bacteria (excluding nitrogen-cycling bacteria and vibrios) increased from 38.75% to 73.43%. However, the planted experimental group maintained a relatively high ratio of nitrogen cycling bacteria at 58%. In the high-concentration experimental group, bacteria related to the initial nitrogen cycle, non-pathogenic vibrios, and pathogenic vibrios were 11.60, 36.28, and 20.14%, respectively. Finally, nitrogen-cycling bacteria were 36.47% in the control group and 37.55% in the planted group. The total number of vibrios decreased by 46.54% in the planted group and 48.01% in the control group, indicating a significant decrease in both experimental groups. However, the residual rate of pathogenic vibrios was 4.48% in the control group and 0.54% in the planted group. Overall, the planted group showed decreasing harmful bacteria and increasing useful bacteria.