• Journal of Korean Society of Environmental Engineers
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• v.31 no.5
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• pp.341-345
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• 2009

Continuous dry anaerobic digestion of organic solid wastes (30% TS, Total Solids) comprised of food waste and paper was performed under mesophilic condition. During the operation, hydraulic retention time (HRT) was decreased as follows: 150 d, 100 d, 60 d, and 40 d, which corresponded to the solid loading rate of 2.0, 3.0, 5.0, and 7.5 kg TS/$m^3$/d, respectively. Volumetric biogas production rate ($m^3$/$m^3$/d) increased as HRT decreased, and the highest biogas production rate of 3.49${\pm}$0.31 $m^3$/$m^3$/d was achieved at 40 d of HRT. At this HRT, high volatile solids (VS) reduction of 76% was maintained, and methane production yield of 0.25 $m^3$/kg $TS_{added}$ was achieved, indicating 67.4% conversion of organic solid waste to bioenergy. The highest biogas production yield of 0.52 $m^3$/kg $TS_{added}$ was achieved at 100 d of HRT, but it did not change much with respect to HRT. For the ease feed pumping, some amount of digester sludge was recycled and mixed with fresh feed to decrease the solid content. Recirculation volume of 5Q was found to be the optimal in this experimental condition. Specific methanogenic activity (SMA) of microorganisms at mesophilic-dry condition was 2.66, 1.94, and 1.20 mL $CH_4$/g VS/d using acetate, butyrate, and propionate as a substrate, respectively.

• Journal of the Korea Organic Resources Recycling Association
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• v.24 no.3
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• pp.75-82
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• 2016

Objective of this experiment was to predict the potential methane production with crop residues at different loading rates. Anaerobic digestion of barley and rapeseed straw substrates for biogas production was performed in Duran bottles at various biomass loading rates with crop residues. Through kinetic model of surface methodology, the methane production was fitted to a Gompertz equation. For the biogas production at mesophilic digestion with crop residues, it was observed that maximum yield was 37.2 and 28.0 mL/g at 6.8 and 7.5 days after digestion with 1% biomass loading rates of barley and rapeseed straws, respectively. For the methane content of mesophilic digestion, there were highest at 61.7% after 5.5 days and 75.0% after 3.4 days of digestion with barley and rapeseed straw on both 5% biomass loading rates, respectively. The maximum methane production potentials were 159.59 mL/g for 1% barley straw and 156.62 mL/g for 3% rapeseed straw at mesophilic digestion. Overall, it would be strongly recommended that biomass loading rate was an optimum rate at mesophilic digestion for using 1% barley and 3% rapeseed straws for feed stocks.

• Applied Chemistry for Engineering
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• v.27 no.5
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• pp.532-536
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• 2016

In this study, single-stage continuous anaerobic reactors to treat sewage sludge were operated under different temperature (55 and $35^{\circ}C$; $R_{TAD}$ and $R_{MAD}$) to evaluate the reactor stability and performance of the thermophilic and mesophilic anaerobic digestion. During the overall digestion, both anaerobic reactors maintained quite stable and constant pH and total alkalinity (TA) values in the range of 6.5-8.0 and 3-4 g $CaCO_3/L$, respectively. After the start-up period, $R_{TAD}$ showed 10% higher VS removal efficiency than that of $R_{MAD}$ ($R_{TAD}$; 43.3%; $R_{MAD}$ : 33.6%). Although organic acids such as acetic and propionic acid were detected in both anaerobic reactors at the start-up period, all organic acids in $R_{TAD}$ and $R_{MAD}$ were consumed at the steady state condition. Also $R_{TAD}$ showed 31.4 % higher methane production rate (MPR) than that of $R_{MAD}$ at the steady state condition ($R_{TAD}$; 243 mL $CH_4/L/d$; $R_{MAD}$ : 185 mL $CH_4/L/d$). Meanwhile, the experimental results indicated similar methane yield between $R_{TAD}$ and $R_{MAD}$.

• Journal of Microbiology and Biotechnology
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• v.17 no.12
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• pp.1976-1982
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• 2007

In the current studies, we characterized the degradation of a hot mixture of benzene and toluene (BT) gases by a thermophilic biofilter using polyurethane as a packing material and high-temperature compost as a microbial source. We also examined the effect of supplementing the biofilter with yeast extract (YE). We found that YE substantially enhanced microbial activity in the thermophilic biofilter. The degrading activity of the biofilter supplied with YE was stable during long-term operation (approximately 100 d) without accumulating excess biomass. The maximum elimination capacity ($1,650\;g{\cdot} m^{-3}{\cdot} h^{-1}$) in the biofilter supplemented with YE was 3.5 times higher than that in the biofilter without YE ($470\;g{\cdot} m^{-3}{\cdot} h^{-1}$). At similar retention times, the capacity to eliminate BT for the YE-supplemented biofilter was higher than for previously reported mesophilic biofilters. Thus, thermophilic biofiltration can be used to degrade hydrophobic compounds such as a BT mixture. Finally, 168 rDNA polymerase chain reaction-DGGE (PCR-DGGE) fingerprinting revealed that the thermophilic bacteria in the biofilter included Rubrobacter sp. and Mycobacterium sp.

• Food Science and Biotechnology
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• v.17 no.1
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• pp.166-171
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• 2008

Abstracts Soybean sprouts which are a popular vegetable in Korea, are produced using the techniques of sous vide. The purpose of this study was to determine the effect of nisin and storage temperature on the microbiological and physicochemical qualities of sous vide processed soybean sprouts during storage in order to improve shelf-life and industrial applications. During storage of the cook-chilled soybean sprouts at $3^{\circ}C$, no development of mesophilic microorganisms was observed. However, at $10^{\circ}C$ storage without nisin, the number of mesophilic microorganisms increased markedly, whereas sprouts stored at the same temperature with nisin showed no observed increase. Psychrotrophic microorganisms, anaerobic microorganisms, and Bacillus cereus all showed similar trends. The ascorbic acid content, following the sequential heat processing of soybean sprouts through blanching and pasteurization decreased markedly during early storage, and stabilized thereafter. During storage, no major changes in the color or ascorbic acid content of samples at either temperature were observed. With regard to microbial and physicochemical qualities, the presences of nisin and storage temperature are important factors for extending shelf-life of soybean sprout.

• Food Science of Animal Resources
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• v.36 no.3
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• pp.300-308
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• 2016

In this study, the effects of grape seed flour on the physical-chemical properties, microbiological and sensory properties of Turkish dry fermented sausage, sucuk, was investigated. After the sausages produced with beef, beef fat, sheep tail fat and spices, they were ripened for 14 d. Then they were vacuum-packaged and stored for 80 d at 4℃. The effects of grape seed flour (GSF; 0%, 0.75%, 1.5%, 3%) on the physical-chemical properties (pH, moisture, fat, protein, free fatty acids, thiobarbituric acids, diameter reduction, ripening yield, instrumental colour), microbiological properties (total aerobic mesophilic and lactic acid bacteria, Enterobacteriaceae, mould and yeast) and sensory properties of the sausages were investigated. Grape seed flour decreased moisture, TBA, diameter reduction, instrumental colour (a, b) values and sensory analysis scores during the ripening period; it also decreased TBA, instrumental colour (L, a, b) values, total aerobic mesophilic and lactic acid bacteria counts during the storage period. It was concluded that grape seed flour has a potential application as an additive in dry fermented sausages.

• 한국신재생에너지학회:학술대회논문집
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• 2010.11a
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• pp.102.2-102.2
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• 2010

We are to evaluate the stabilization technology of actual biogas plant facilities, which is operating currently. It describes the traits of the consistent facilities of mesophilic anaerobic digestion using Unison Biogas plant Recovery system(UBR). Also the economical efficiency is examined with the electric power sales earnings and applying the deserted heating by generating electric power, which is generated by operated combined heat and power using biogas produced by mesophilic anaerobic digestion. We have generated the 481,113kw for electric power and 1,376Gcal for thermal energy simultaneously. If these electric power and thermal energy are converted into diesel, we can achieve savings equal to 114,300L, and 152,109L in the quantity of heat. Finally, if CDM, RPS, liquid fertilizer sales business, etc. is activated, the earnings will be expected to improve dramatically and is considered to contribute a drop of the greenhouse gas.

• Journal of Biosystems Engineering
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• v.37 no.1
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• pp.58-64
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• 2012

Purpose: The objective of this study was to investigate the feasibility of anaerobic digestion of Chinese cabbage waste juice (CCWJ) and swine manure(SM). Methods: The anaerobic digestion test was conducted under batch and continuous conditions at mesophilic temperature ($36-38^{\circ}C$). The batch test was divided into Experiment I and II. In the Experiment I, biogas potential and production rate of CCWJ was evaluated. In Experiment II the effect of F/M ratio (2.0, 3.2, 4.9) at mixture ratio of 25:75(CCWJ: SM, % vol. basis) on biogas yield was studied. Results: CCWJ produced biogas and methane yield of 929 and 700 mL/g VS added respectively. The biogas yield from the mixture of CCWJ and SM was almost same at F/M ratio of 2.0 and 3.2 but dropped by 14% when F/M ratio increased from 3.2 to 4.9. In continuous test the mixture of CCWJ and SM (25:75, % vol. basis) produced biogas yield of 352 mL/g VS added which is around 11% higher compared to biogas yield from SM alone. Addition to biogas yield digester performance was also improved with co-digestion of CCWJ with SM. Conclusions: The results showed that the anaerobic digestion of CCWJ with SM could be promising for improving both the biogas yield and digester performance at mesophilic temperature.

• Journal of Microbiology and Biotechnology
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• v.29 no.12
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• pp.1993-2005
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• 2019

Thermophilic anaerobic digestion (TAD) is characterized by higher biogas production rates as a result of assumedly faster microbial metabolic conversion rates compared to mesophilic AD. It was hypothesized that the thermophilic microbiome with its lower diversity than the mesophilic one is more susceptible to disturbances introduced by alterations in the operating factors, as an example, the supply of nitrogen-rich feedstock such as poultry manure (PM). Laboratory scaled TAD experiments using cattle slurry and increasing amounts of PM were carried out to investigate the (in-) stability of the process performance caused by the accumulation of ammonium and ammonia with special emphasis on the microbial community structure and its dynamic variation. The results revealed that the moderate PM addition, i.e., 25% (vol/vol based on volatile substances) PM, resulted in a reorganization of the microbial community structure which was still working sufficiently. With 50% PM application, the microbial community was further stepwise re-organized and was able to compensate for the high cytotoxic ammonia contents only for a short time resulting in consequent process disturbance and final process failure. This study demonstrated the ability of the acclimated thermophilic microbial community to tolerate a certain amount of nitrogen-rich substrate.

• Journal of Korean Society of Water and Wastewater
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• v.25 no.3
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• pp.343-348
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• 2011

Since 2005, food waste has been separately collected and recycled to animal feed or aerobic compost in South Korea. However, the conventional recycling methods discharge process wastewater, which contain pollutant equivalent to more than 50% of food waste. Therefore, anaerobic digestion is considered as an alternative recycling method of food waste to reduce pollutant and recover renewable energy. Recent studies showed that hydrogen can be produced at acidogenic stage in two-stage anaerobic digestion. In this study, the authors investigated the effects of pretreatment time and pH low set value on continuous mesophilic hydrogen fermentation of food waste. Food waste was successfully converted to $H_2$ when heat-treated at $70^{\circ}C$ for 60 min, which was milder than previous studies using pH 12 for 1 day or $90^{\circ}C$. Organic acid production dropped operational pH below 5.0 and caused a metabolic shift from $H_2/butyrate$ fermentation to lactate fermentation. Therefore, alkaline addition for operational pH at or over 5.0 was necessary. At pH 5.3, the result showed that the maximum hydrogen productivity and yield of 1.32 $m^3/m^3$.d and 0.71 mol/mol $carbohydrate_{added}$. Hydrogen production from food waste would be an effective technology for resource recovery as well as waste treatment.