• Title/Summary/Keyword: Membrane vesicle

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Measurement of Photospetroscpopies by Anesthetics in Purple Membrane and Red Membrane (Purple Membrane과 Red Membrane에서 마취제에 의한 분광학적 측정)

  • Kim, Ki-Jun;Jeong, Hyeon-ghak;Kim, Juhan;Song, Hui-jun
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.2
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    • pp.472-477
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    • 2018
  • The excess molar volumes of a general anesthetics on Purple membrane and Red membrane separated by extraction in Halobacteriun Halobium and in suspensions of vesicle have been determined at $25^{\circ}C$, it was used a excess volume dilatometer. The anesthesia characteristics of general anesthetics, Propofol was fined by our study to correlate with excess molar volume. Excess volume changes of the vesicle measured by excess volume dilatometer, which is an important amino acid and lipid in the purple membrane and red membrane by means of specific weight in Halobacteriun Halobium, were studied by absorption intensity at 280 nm and 330 nm. The particle size analysis and relative turbidity of Purple membrane and Red membrane by means of Propofol were measured for mechanical properties. In the samples where Propofol is incoporated in vesicle, especially, the excess molar volume of PM + RM + Propofol is the greatest than the excess molar volumes of PM, and RM.

Isolation and characterization of the outer membrane vesicle (OMV) protein from Vibrio anguillarum O1 (Vibrio anguillarum O1이 생산하는 Outer Membrane Vesicle (OMV)의 분리 및 OMV 내의 단백질 특성)

  • Hong, Gyeong-Eun;Kim, Dong-Gyun;Min, Mun-Kyeong;Kong, In-Soo
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.2
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    • pp.123-125
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    • 2007
  • Vibrio anguillarum is a gram-negative bacterium that causes vibriosis in approximately 80 different fish species. V. anguillarum produces several exotoxins are correlated with the pathogenesis of vibriosis. This study is focused on the composition of the outer membrane vesicle. Most of gram-negative bacteria produce outer membrane vesicle (OMV) during cell growth. OMV was formed from the outer membrane surface of cell and than released to extracellular environment. OMV consists of outer membrane lipids, outer membrane protein (OMP), LPS, and soluble periplasmic components. Also, they contain toxins, adhesions, and immunomodulatory. Many gram-negative bacteria were studied out forming OMV. In Vibrio sp., formation of OMV by electron microscopy has been reported from V. cholerae and V. parahaemolyticus. In present study, we isolated OMV from V. anguillarum and OMV protein was separated by SDS-PAGE. Magor band was sliced and analyzed by MALDI-TOF. The major protein band of 38kDa was identified as OmpU by MALDI-TOF MS analysis.

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Energy Transfer of Methylene Blue on the Purple Membrane Incorporated into $L-{\alpha}-lecithin$ Vesicle by Photochemical Reaction Differential Scanning Calorimetry (Purple Membrane으로 재구성된 $L-{\alpha}-lecithin$ Vesicle에서 Photochemical Reaction Differential Scanning Calorimetry에 의한 Methylene Blue의 에너지 전달)

  • Kim, Ki-Jun;Sung, Ki-Chun;Lee, Hoo-Seol
    • Journal of the Korean Applied Science and Technology
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    • v.13 no.3
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    • pp.127-136
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    • 1996
  • Thermograms of methylene blue(MB) in $L-{\alpha}-lecithin$ vesicle and incorporated purple membrane vesicle(InPM) systems have been studied by photochemical reaction differential scanning calorimetry at $25{\sim}55^{\circ}C$. Phase transition temperatures of lecithin vesicle, purple membrane(PM), and InPM were found to be independent of illumination of light(436nm) at $39{\sim}40^{\circ}C$, but endothermic phase transition was found in InPM vesicle. In MB-InPM system, endothermic phase transition was found on unillumination of light at $40{\sim}42^{\circ}C$, but exothermic phase transition was found on steady illumination of light at $48{\sim}52^{\circ}C$. It was estimated that the light energy absorbed from MB on vesicular surface was transferred to PM, and the transferred energy was redistributed to hydrophobic site of membrane. Therefore, the exothermic phase transition was measured at high temperature because of the increased hydrophobicity of acyl chain.

A Study on Change of Excess Volume in Membrane of Holobacterium Holobium (Halobacterium Halobium의 Membrane에서 잉여부피 변화에 관한 연구)

  • Kim, Ki-Jun;Lee, Joo-Youb
    • Journal of the Korean Applied Science and Technology
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    • v.28 no.2
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    • pp.247-250
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    • 2011
  • The excess volumes of mixing of benzyl alcohol and halothane in vesicle and in suspensions of several lipids have been determined at $25^{\circ}C$ it using a excess volume dilatometer. The potency of general anesthetics has long been known to correlate with lipid solubility. Denaturations of the vesicle, which is a sole membrane protein in the purple membrane of Halobacteriun Halobium, were studied by absorption changes at 280 nm and fluorescence changes at 330 nm and excess volume dilatometer. The particle size analysis of viscous polymer solutions by diffusional interchange is the key step by measurement. The excess volume of mixing in chitosan was found to be negative, whereas them of purple membrane, Halobacteriun Halobium and red membrane were positive in benzyl alcohol and halothane. This result was confirmed as Miller's supposition.

Synthesis of Cholesterol-Containing Molecular Multilayer Structure (콜레스테롤을 갖는 분자 다층 구조의 합성)

  • 신재섭
    • Membrane Journal
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    • v.6 no.4
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    • pp.213-218
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    • 1996
  • Cholesterol-containing monomeric surfactants were synthesized, and sonicated in water to form a vesicle solution. This vesicle solution was dried to construct a membrane which had a molecular multilayer structure. Using UV irradiation the surfactants in this membrane were polymerized and molecular weight was determined. Using a X-ray diffractomelet, the thickness of one layer and the regularity of the multilayer were measured.

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Kinetics of Organic ion Transport Across Rabbit Renal Brush Border and Basolateral Membrane Vesicles (가토 신피질 Brush Border Membrane과 Basolateral Membrane Vesicle에서 유기이온의 이동에 대한 동력학적 분석)

  • Kim, Yong-Keun;Woo, Jae-Suk;Lee, Sang-Ho
    • The Korean Journal of Physiology
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    • v.21 no.2
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    • pp.273-282
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    • 1987
  • 가토 신피질에서 분리한 brush border membrane (BBM)과 basolateral membrane vesicle (BLM)에서 유기 음이온인 p-aminohippuric acid (PAH)와 유기 양이온인 tetraethylammonium (TEA)의 이동에 대한 동력학적 분석을 하였다. BLM에서 PAH에 대한 Km과 Vmax값은 각각 $0.34{\pm}0.02\;mM$$0.22{\pm}0.07\;nmol/mg\;protein/20s$였으며 BBM에서 각 값은 $8.46{\pm}0.57\;mM$$4.43{\pm}0.40\;nmol/mg\;protein/20s$였다. BLM에서 용액내 Na의 제거는 PAH에 대한 Km 값에는 영향없이 Vmax 값을 변화시켰다. BBM에서 TEA이동에 대한 Km과 Vmax 값은 각각 $0.55{\pm}0.15\;mM$$1.04{\pm}0.23\;nmol/mg\;protein/20s$였으며 BLM에서 각 값은 $0.46{\pm}0.04\;mM$$0.61{\pm}0.06\;nmol/mg\;protein/20s$였다. BLM에서 측정한 유기 이온들의 이동에 대한 Km 값이 신절편이나 분리된 tubule에서 보고된 값과 일치함을 보였으며 이러한 결과는 신세뇨관 세포막을 통한 유기 이온들의 이동 특성이 membrane vesicle을 분리하는 과정에서 변하지 않았음을 가르킨다.

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Synthesis of Two Dimensional Polymer Network (이차원 구조를 갖는 고분자의 합성)

  • Shin, Jae Sup
    • Applied Chemistry for Engineering
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    • v.9 no.5
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    • pp.763-767
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    • 1998
  • Cholesterol-containing surfactant was synthesized, and it was sonicated with monomer [tetradeca(ethylene glycol) diacrylate] in water to from a vesicle solution. This vesicle solution was dried to construct a membrane which had a molecular multilayer structure. The monomer which stay in this membrane was polymerized with photoinitiation, and then surfactant was extracted by organic solvent. The physical properties of the two dimensional polymer network were measured, and these physical properties are tensile strength, elongation, and swelling.

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Aggregation of Methylene Blue on the L-${\alpha}$-lecithin Bilayer Membrane (L-${\alpha}$-lecithin 이중층막에서 Methylene Blue의 회합)

  • Lee, Hong;Lee, Hoo-Seol
    • Journal of the Korean Chemical Society
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    • v.35 no.6
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    • pp.612-616
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    • 1991
  • Metachromatic behavior of methylene blue (MB) in solutions of aqueous sodium dodecyl sulfate (SDS), chondroitin sulfate and L-${\alpha}$-lecithin vesicle at $18~52^{\circ}$C has been studied by absorption spectroscopy. MB was clustered in the matrix of the vesicle with high concentration of L-${\alpha}$-lecithin. The metachromasy of MB was found to be independent of phase transition temperature of vesicles. These results suggest that the dyes were aggregated on the hydrophilic surfaces of vesicle. In the vesicular system, the metachromatic effect of MB was dramatically decreased in the presence of hexadecyltrimethylammonium bromide (CTAB) than SDS. It is estimated that the intercalation site of these surfactants on vesicle surfaces was different, that is, the intercalation of CTAB was more effective than that of SDS.

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Synthesis of Ultrathin Polymer Films by Self Assembly (자기 집합에 의한 고분자 초박막의 합성)

  • Shin, Jae Sup
    • Applied Chemistry for Engineering
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    • v.7 no.6
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    • pp.1142-1146
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    • 1996
  • Cholesterol-containing surfactant was synthesized, and it was sonicated with monomer in water to form a vesicle solution. This vesicle solution was dried to construct a membrane which had a molecular multilayer structure. Using UV irradiation the monomer in this membrane were polymerized, and then surfactant was extracted by organic solvent. Using a X-ray diffractometer, the thickness of one layer and the regularity of the multilayer were measured. And scanning electron microscopy was conducted for fractured polymer film.

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Catalytic Hydrolysis of p-Nitrophenyl Palmitate in Aqueous Dipalmitoyl Phosphatidyl Choline Bilayer Membrane (Dipalmitoyl Phosphatidyl Choline Bilayer Membrane 촉매에 의한 para-Nitrophenyl Palmitate의 가수분해 반응)

  • Kim, Ki-Jun;Lee, Hoo-Seol
    • Journal of the Korean Applied Science and Technology
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    • v.25 no.1
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    • pp.48-51
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    • 2008
  • Dipalmitoyl phosphatidyl choline and p-nitrophenyl palmitate were directly sonicated in acidic water for 6 minutes to give clear stock solutions. The catalytic hydrolysis of p-nitrophenyl palmitate was studied at $30-50^{\circ}C$ in the presence of unilamellar vesicle and mixture of unilamellar and multilamellar aggregates. The difference of reaction rate between unilamellar and multilamellar was observed. The rate of unilamellar reaction compared to the rate of mixture reaction showed more catalytic effect. The phase transition temperature of vesicle was measured at $37-44^{\circ}C$.