• Archives of Pharmacal Research
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• 제21권3호
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• pp.241-247
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• 1998

This study was designed to determine biochemical and pharmacological properties of a newly synthesized benzimidazole derivative, 2-amino-4, 5-dihydropyrido [1, 2-a] thiazolo [5, 4-g] benzimidazole (YJA20379-5) in vitro and in vivo. In the leaky membrane vesicles of pig gastric mucosa, YJA20379-5 inhibited the $K^+$-stimulated $H^+$, $K^+$-ATPase activity in a concentration- and time-dependent manner, with $IC_{50}$ values being $43{\mu}\textrm{M}$ and $43{\mu}\textrm{M}$ at pH 6.4 and 7.4, respectively. YJA20379-5, given intraduodenally, had a potent inhibitory effect on the gastric acid secretion in pylorus-ligated rats. The $ED_{50}$ value for acid secretion was 15.4 mg/kg. YJA20379-5, administered orally, also suppressed gastric damages induced by water-immersion stress, indomethacin and ethanol, and duodenal damage induced by mepirizole in rats; the $ED_{50}$ values were 17.6, 4.7, 3.0 and 18.7 mg/kg, respectively. Furthermore, repeated oral administration of YJA20379-5 accelerated the spontaneous healing of acetic acid-induced gastric ulcers in rats. It is concluded that the a-ntisecretory activity of YJA20379-5 appears to be associated with inhibition of $H^+$, $K^+$-ATPase, while its antigastric and antiduodenal lesion activities are primarily related to the antisecretory effect.

• 한국임상수의학회지
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• 제34권4호
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• pp.261-267
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• 2017

The aim of this study was to investigate the antibacterial properties of chitosan silver nanocomposites (CAgNCs) using pathogenic Vibrio ichthyoenteri as a bacterial model. Results of agar disc diffusion and turbidimetric assays showed that CAgNCs could inhibit the growth of V. ichthyoenteri in concentration dependent manner. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CAgNCs were 75 and $125{\mu}g/mL$, respectively. Furthermore, CAgNCs treatment induced the reactive oxygen species (ROS) level in V. ichthyoenteri cells in concentration and time dependent manner, suggesting that it generates oxidative stress, leading to bacterial cell death. The field emission scanning electron microscope (FE-SEM) images of CAgNCs treated V. ichthyoenteri exhibited strong cell membrane damage than un-treated control bacteria. MTT assay results showed the highest cell viability (22%) at $75{\mu}g/mL$ of CAgNCs treated bacteria samples. The results from this study suggest that CAgNCs is a potential antibacterial agent to control fish pathogenic bacteria.

• Journal of Advanced Marine Engineering and Technology
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• 제39권1호
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• pp.19-27
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• 2015

최근에 국제해사기구(IMO)에서 선박 평형수 처리장치(Ballast water treatment system, BWTS)와 관련된 국제 규정을 강화함에 따라 이에 대응할 수 있는 BWTS 기술개발의 중요성이 대두되고 있다. 지금까지는 세계 각국에서 선박 평형수 처리기술을 처리방식에 따라 크게 물리적 방식(막분리, 원심분리, 자외선 등)과 화학적 처리방식(염소처리, 오존처리, 화확약품 등)으로 개발하여 왔으나, 기존의 기술 방식들은 추가적인 구동전력, 낮은 생산성, 잔류처리 문제 등으로 기술적인 보완이나 대안이 요구되고 있다. 본 연구에서는 기존과는 다른 새로운 방식의 기계식 방식 평형수 처리장치의 설계를 위한 유동해석 연구이다. 고속 회전하는 몇 가지 디스크 형상에 따라 유동에서 발생하는 여러 가지 유동특성을 전산유체해석(CFD)를 통하여 분석하였다. 평판형 및 계단형 형상에서는 반경을 따라서 국부적으로 공동현상이 발생가능하며, 원주형에서는 전단응력 강도를 높일 수 있었다.

• 대한관절경학회지
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• 제1권1호
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• pp.112-115
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• 1997

저자들이 전방십자인대 파열과 함께 전방 불안정성을 보인 2례를 보존적으로 치료하여 좋은 임상적 결과를 얻은 바를 고려할 때, 급성기에 전방십자인대가 파열된 경우에도 전방십자인대를 싸고 있는 활액막이 파열되어 없어지지 않고 비교적 연속성이 잘 유지된 경우에는 보존적으로 치료함으로써 좋은 결과를 기대할 수 있을 것으로 사료되며 아울러 십자인대가 손상받았을 때 스스로 복원 능력이 있는 지에 대하여 새로운 연구가 필요하리라 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권2호
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• pp.245-251
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• 2006

The aim of this study was to investigate the effects of caponization on the bone characteristics, biomechanical property and histology in Taiwan country chickens fed to market age of 26 wks. Male Taiwan country chickens $D{\times}L_2$ were caponized or sham-operated at 8 wks of age, and selected healthy sham-operated and completely caponized chickens (prominent degenerated comb) were selected at 16 wks old and fed to 26 wks old for the trials. Fifteen intact male chickens (Intact), sham-operated chickens (Sham) and caponized chickens (Capon) were assigned for trial 1, and sixteen Intact and Capon were assigned for trial 2. Results in trial 1 showed that the abdominal fat and relative abdominal fat weights of Capon were significantly heavier than Intact and Sham (p<0.05), while the tibia weight and relative weight were the lightest (p<0.05). The tibia breaking strength, bending moment and stress of Capon were the poorest among groups (p<0.05). The trial 2 produced the similar observation that Capon were significantly lighter than Intact (p<0.05) in the tibia weight, relative tibia weight and their biomechanical properties. On histological determinations, Capon showed a thinner cartilage end and fewer chondrocytes (about 50%) and trabecular, and bigger marrow cavity; while decreased hemopoietic cells number with increased adipocytes than Intact observed by H&E stain and at low magnification. At high magnification, Capon showed a decrease in the chondrocyte size by 33 to 50%, with smaller nucleus located near the cell membrane, and exhibited monocellular form chondrocytes. Capon also showed a less strongly acidic sulfated mucosubstance with weaker dyeing property within cartilage zone, and smaller chondrocytes size by Alcian blue stain.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2000년도 추계학술대회논문집A
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• pp.105-110
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• 2000

The Micromechanics test is new test method which uses comparatively smaller specimen than that required in conventional material tests. There are several methods, such as small-specimen creep test, the continuous indentation test, and small punch(SP) test. Among them, the small punch(SP) test method has been applied to many evaluation fields, such as a ductile-brittle transition temperature, stress corrosion cracking, hydrogen embrittlement, and fracture properties of advanced materials like FGM or MMC. In this study, the small punch(SP) test is performed to evaluate the mechanical properties at high/low temperature from $-196^{\circ}C$ to $650^{\circ}C$ and the material degradation for virgin and aged materials of 9Cr1MoVNb steel which has been recently developed. The ${\Delta}P/{\Delta}{\delta}$ parameter defined a slope in plastic membrane stretching region of SP load-displacement curve decreases according to the increase of specimen temperature, and that of aged materials is higher than the virgin material in all test temperatures. And the material degradation degrees of aged materials with $630^{\circ}C$ -500hrs and $630^{\circ}C$ -1000hrs are $36^{\circ}C$ and $38^{\circ}C$ respectively. These behaviors are good consistent with the results of hardness($H_v$) and maximum displacement(${\delta}_{max}$).

• 전력전자학회논문지
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• 제15권1호
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• pp.75-85
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• 2010

본 연구에서는 비선형 부하에서 발생되는 기본파 무효전력 및 고조파를 보상하기 위한 종전의 전압형 및 전류형 PWM 능동전력필터를 대체 할 수 있는 Z-소스 인버터 토폴로지의 하이브리드 능동전력필터에 대하여 고찰하였다. Z-소스 토폴로지의 능동전력필터의 보상 DC전원으로는 PEMFC가 사용되며, Z-소스 인버터의 shoot-through 듀티비의 조절에 의하여 낮은 연료전지의 전압을 높은 보상 전압으로 부스트 한다. 제안된 시스템은 병렬형 Z-소스 능동전력필터와 7차 고조파 (420Hz) 동조 필터로 구성되며, 이 구성에 의하여 Z-소스 능동전력필터의 스위치 디바이스의 전압 스트레스는 감소된다. 제안된 Z-소스 하이브리드 능동전력필터의 보상 알고리즘으로는 전류 동기 검출법이 사용되었다. 3상 220V/60Hz, 25A급 비선형 다이오드 부하 조건하에서 PSIM 시뮬레이션을 수행하였으며, 정상상태 및 과도상태에서의 제안된 시스템의 보상 성능을 파악하였다.

• Restorative Dentistry and Endodontics
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• 제27권5호
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• pp.543-551
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• 2002

Nitric oxide (NO) is a small molecule (mol. wt. 30 Da) and oxidative free radical. It is uncharged and can therefore diffuse freely within and between cells across membrane. Such characteristics make it a biologically important messenger in physiologic processes such as neurotransmission and the control of vascular tone. NO is also highly toxic and is known to acts as a mediator of cytotoxicity during host defense. NO is synthesized by nitric oxide synthase (NOS) through L-arginine/nitric oxide pathway which is a dioxygenation process. NO synthesis involves several participants, three co-substrates, five electrons, five co-factors and two prosthetic groups. Under normal condition, low levels of NO are synthesized by type I and III NOS for a short period of time and mediates many physiologic processes. Under condition of oxidant stress, high levels of NO are synthesized by type II NOS and inhibits a variety of metabolic processes and can also cause direct damage to DNA. Such interaction result in cytostasis, energy depletion and ultimately cell death. NO has the potential to interact with a variety of intercellular targets producing diverse array of metabolic effects. It is known that NO is involved in hemodynamic regulation, neurogenic inflammation, re-innervation, management of dentin hypersensitivity on teeth. Under basal condition of pulpal blood flow, NO provides constant vasodilator tone acting against sympathetic vasoconstriction. Substance P, a well known vasodilator, was reported to be mediated partly by NO, while calcitonin-gene related peptide has provided no evidence of its relation with NO. This review describes the roles of NO in dental pulp in addition to the known general roles of it.

• 대한약침학회지
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• 제17권1호
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• pp.59-69
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• 2014

Objectives: In homeopathy, it is claimed that more homeopathically-diluted potencies render more protective/curative effects against any disease condition. Potentized forms of Condurango are used successfully to treat digestive problems, as well as esophageal and stomach cancers. However, the comparative efficacies of Condurango 6C and 30C, one diluted below and one above Avogadro's limit (lacking original drug molecule), respectively, have not been critically analyzed for their cell-killing (apoptosis) efficacy against lung cancer cells in vitro, and signalling cascades have not been studied. Hence, the present study was undertaken. Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were conducted on H460-non-small-cell lung cancer (NSCLC) cells by using a succussed ethyl alcohol vehicle (placebo) as a control. Studies on cellular morphology, cell cycle regulation, generation of reactive oxygen species (ROS), changes in mitochondrial membrane potential (MMP), and DNA-damage were made, and expressions of related signaling markers were studied. The observations were done in a "blinded" manner. Results: Both Condurango 6C and 30C induced apoptosis via cell cycle arrest at subG0/G1 and altered expressions of certain apoptotic markers significantly in H460 cells. The drugs induced oxidative stress through ROS elevation and MMP depolarization at 18-24 hours. These events presumably activated a caspase-3-mediated signalling cascade, as evidenced by reverse transcriptase-polymerase chain reaction (RT-PCR), western blot and immunofluorescence studies at a late phase (48 hours) in which cells were pushed towards apoptosis. Conclusion: Condurango 30C had greater apoptotic effect than Condurango 6C as claimed in the homeopathic doctrine.

• 한국작물학회지
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• 제50권2호
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• pp.84-90
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• 2005

The effects of UV-B radiation on the seedling growth, carbohydrate metabolism and antioxidants activities of rice (Oryza sativa L.) were investigated under environmentally controlled chamber. Supplementary UV­B radiation reduced dry matter as well as leaf area, there­fore, relative growth rates (RGR) of seedlings were decreased by up to half compared to control. Photosynthetic products such as soluble sugars and starch were rapidly and significantly reduced by within 1 day of enhanced UV-B radiation due to the inhibition and degradation of photosynthetic processes and thylakoid membrane integrity. In our study, nonstructural carbohydrate levels were proved to be a main indicator on UV-B­induced stress. The behavior of SOD, CAT, APX and POD activities was monitored in the leaves of rice seedlings subjected to UV-B radiation. Under UV-B treatments, SOD activity was initially increased, whereas CAT and POD activities were slowly and slightly increased. However, APX activity showed no presumable results with an increase of UV-B dose. In leaves of rice seedlings, supplementary UV-B radiation caused an increase in free putrescine and spermidine, however spermine remained unaltered, although 24-hrs UV-B treatment slightly increased. This result presumes that an excess UV-B dose may induce ethylene biosynthesis (senescence) rather than polyamine biosynthesis (defense).