• Journal of Crop Science and Biotechnology
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• 제11권2호
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• pp.91-100
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• 2008

Melting curve analysis of fluorescently labeled DNA fragments is used extensively for genotyping single nucleotide polymorphism(SNP). Here, we evaluated a SNP genotyping method by melting curve analysis with the two probe chemistries in a 384-well plate format on a Roche LightCycler 480. The HybProbe chemistry is based on the fluorescence resonance energy transfer(FRET) and the SimpleProbe chemistry uses a terminal self-quenching fluorophore. We evaluated FRET HybProbes and SimpleProbes for two SNP sites closely linked to two quantitative trait loci(QTL) for southern root-knot nematode resistance. These probes were used to genotype the two parents and 94 $F_2$ plants from the cross of PI 96354$\times$Bossier. The SNP genotypes of all samples determined by the LightCycler software agreed with previously determined SSR genotypes and the SNP genotypes determined on a Luminex 100 flow cytometry instrument. Multiplexed HybProbes for the two SNPs showed a 98.4% success rate and 100% concordance between repeats two of the same 96 DNA samples. Also, we developed a HybProbe assay for the Rcs3 gene conditioning broad resistance to the frogeye leaf spot(FLS) disease. The LightCycler 480 provides rapid PCR on 384-well plate and allows simultaneous amplification and analysis in approximately 2 hours without any additional steps after amplification. This allowed for a reduction of the potential contamination of PCR products, simplicity, and enablement of a streamlined workflow. The melting curve analysis on the LightCycler 480 provided high-throughput and rapid SNP genotyping and appears highly effective for marker-assisted selection in soybean.

• Korean Chemical Engineering Research
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• 제46권2호
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• pp.340-347
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• 2008

용해공정의 경우, 용해물을 균일한 상태로 유지하고 용해로에 투입되는 연료의 연소상황을 분석하기 위해 폐가스 유속을 측정한다. 현재 시중에 많은 종류의 기체유속 측정장치들이 있지만, 용해공정에서 발생하는 폐가스는 고온이고, 저속이며, dust를 많이 갖고 있어 기존의 측정장치들은 이런 조건에 적합하지 않다. 따라서 저속에 반응할 만큼 충분히 정밀하고 고온에 견딜 수 있는 새로운 방식의 측정장치를 제작하였다. 현장테스트 결과, 제작된 측정 장치는 유속의 작은 변화에도 반응할 정도로 충분히 민감하고 온도변화를 빠르게 감지하기 때문에 용해로의 온도제어에 도움을 줄 것으로 기대된다.

• 한국세라믹학회지
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• 제48권2호
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• pp.168-172
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• 2011

Alumina powder was added in a general porcelain (Backja) with clay, feldspar and quartz contents to promote the mullite ($3Al_2O_3{\cdot}2SiO_2$) generation in the porcelain. Low melting materials ($B_2O_3(450^{\circ}C)$, $MnO_3(940^{\circ}C)$, CuO($1080^{\circ}C$)) were doped at ~3 wt% to modify the sinterability of porcelain with a high alumina contents and promote the mullite generation. Green body was made by slip casting method with blended slurry and then, they were fired at $1280^{\circ}C$ for 1hr by a $2^{\circ}C/min$. Densifications of samples with high alumina contents (20~30 wt%) were impeded. As the doping contents of low melting materilas increased, the sinterability of samples was improved. The shrinkage rate and bulk density of samples were improved by doping with low melting materials. Mullite phase increased with increasing the low melting contents in the phase analyses. This means lots of alumina and quartz were transformed into mullite phase by low melting contents doping. In the results, high bending strength of samples with high alumina contents was accomplished by improving the densification and mullite generation in the porcelain.

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2009년 추계학술발표대회
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• pp.73-73
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• 2009

In this sutdy, a new class ACA(Anisotropic Conductive Adhesive) with low-melting-point alloy(LMPA) and self-organized interconnection method were developed. This developed self-organized interconnection method are achieved by the flow, melting, coalescence and wetting characteristics of the LMPA fillers in ACA. In order to observe self-interconnection characteristic, the QFP($14{\times}14{\times}2.7mm$ size and 1mm lead pitch) was used. Thermal characteristic of the ACA and temperature-dependant viscosity characteristics of the polymer were observed by differential scanning calorimetry(DSC) and torsional parallel rheometer, respectively. A electrical and mechanical characteristics of QFP bonding were measured using multimeter and pull tester, respectively. Wetting and coalescence characteristics of LMPA filler particles and morphology of conduction path were observed by microfocus X-ray inspection systems and cross-sectional optical microscope. As a result, the developed self-organized interconnection method has a good electrical characteristic($2.41m{\Omega}$) and bonding strength(17.19N) by metallurgical interconnection of molten solder particles in ACA.

• 한국정밀공학회지
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• 제32권12호
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• pp.1023-1029
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• 2015

Laser-assisted machining (LAM) is one of the most effective methods for enhancing the machinability of difficult-to-cut materials, such as titanium alloys and various ceramics, and has been studied by many researchers. LAM is a method that facilitates machining by softening a workpiece using a laser heat source. The advantages of the LAM process are decreases in tool wear, cutting force, and surface roughness. However, when the material is over-heated, melting or burning can occur. This study analyzed the heat source distribution with regard to overlapping of preheating on the laser heating path with an acute angle, a right angle and obtuse angles. Then, a power reduction method was proposed to reduce the melting and burning of the workpiece.

• 한국결정성장학회지
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• 제6권4호
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• pp.620-626
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• 1996

자체 제작한 고주파 유도 가열 Czochralski 장치를 이용하여 조화용융조성(congruently melting composition)의 undoped 및 MgO.doped 단결정을 육성하였다. 최적육성조건을 확립하였으며, 보상가열전압조정방식을 이용하여 약 ${\pm}5\;%$ 이내의 범위로 직경제어하는데 성공하였다. 또한, 첨가된 $Mg^{2+}$ 이온이 ferroelectric domain 형성에 미치는 영향을 전자현미경(SEM)등으로 분석하였다.

• 한국도로학회논문집
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• 제15권2호
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• pp.29-37
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• 2013

PURPOSES : This study aims to review the possibility of developing a road snow-melting system that can prevent slip accidents by maintaining a constant temperature of the winter roads and enhance performance of structures, including improvement of compressive strength by mixing carbon nanotube (hereafter referred to as CNT) with cement paste, the basic material. METHODS : To achieve the above purpose, an experiment was conducted by mixing power-type CNT and wrap-type CNT up to cement paste formulation by weight of 0.0wt%~4.1wt% in accordance with "KS L ISO 679(of cement strength test method)", and compressive strength was measured at 28 days of curing. In addition, the volume resistivity of the specimen was measured to test thermal and electrical characteristics, and the rate of temperature changes in specimen surface by power consumption was measured by passing electricity through the cross-sections of the specimen. Meanwhile, the criteria for checking the performance as a road snow-melting system was determined as volume resistivity of $100{\Omega}{\cdot}cm$ or less. RESULTS : A comparative analysis between specimen with 0wt% CNT content in plain status and specimen containing various types of CNTs was carried out. From its results, it was found that compressive strength increased approximately 19%, showing the highest rate when 0.2wt% of wrap-type CNT was contained, but volume resistivity of $100{\Omega}{\cdot}cm$ or less appeared only in specimens containing more than 0.2wt% CNT. In addition, it was observed that the surface temperature increased by $4.62^{\circ}C$ per minute on average in specimens containing 3.2wt% CNT. CONCLUSIONS : In this study, CNT was examined as an underlying material for a road snow-melting system, and the possibility of developing the road now-melting system was reviewed by conducting various experiments using CNT-Cement composites. From the experimental results, the specimens were found to have a superior performance when compared to the existing road snow-melting systems that place the heat transfer medium such as copper on the road. However, satisfactory strength performance were not obtained from the specimen containing CNT(2.0% or more) that functions as a heating element, which leads to the need for reviewing methods to increase the strength by using plasticizer or admixture.

• 설비공학논문집
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• 제17권3호
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• pp.223-231
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• 2005

When test tubes for PCM with melting point lower than a room temperature are installed vertically as the T-history method proposes, there exists a temperature distribution in the longitudinal direction by natural convection, which lowers the precision of measured heat-of-fusion. The purpose of the present work is to improve the precision by arranging the test tubes horizontally, while maintaining the simplicity and convenience. Assuming that the amount of heat transfer is very small through the vapor space formed in the upper part of the tubes by volumetric change, the obtained value by the T-history method using the latent heat period bounded by two inflection points is in good agreement with that of the literature. Also, the scattering of measured values by the proposed method decreases markedly compared to that of vertical positioning.

• 한국전산구조공학회논문집
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• 제25권5호
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• pp.439-446
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• 2012

본 논문은 1차원 자유경계문제 해석의 정확도 향상을 위해 이동최소제곱 차분법을 이용하여 이동경계의 위상변화를 implicit하게 추적하는 기법을 제시한다. 기존의 이동최소제곱 차분법은 이동경계의 위치를 explicit하게 진전시켜 반복계산은 필요없지만 해의 정확도 감소를 피할 수 없었다. 그러나 본 연구에서 제시한 implicit 기법은 전체 계방정식이 비선형 시스템이 되어 반복계산 과정이 필요하지만, 실제로 수치예제를 통해 검증해 본 결과 계산량의 큰 증가없이 해석의 정확도를 획기적으로 향상시켰다. 이동하는 미분불연속 특이성을 갖는 융해(melting)문제를 수치계산한 결과, implicit 이동최소제곱 차분법을 통해 2차정확도를 얻을 수 있음을 보였다.

• Korean Chemical Engineering Research
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• 제62권1호
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• pp.53-69
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• 2024

The solubility of Cloxacillin sodium in ethanol, 1-propanol, isopropanol, and acetone solutions was measured at different temperatures. The melting property was also tested by using a differential scanning calorimeter (DSC). Then, the solubility data were fitted using Apelblat equation and λh equation, respectively. The Wilson model and NRTL model were not utilized to correlate the test data, since Cloxacillin sodium will decompose directly after melting. For comparison purposes, the four empirical models, i.e., Apelblat equation, λh equation, Wilson model and NRTL Model, were evaluated by using 1155 solubility curves of 103 solutes tested under different monosolvents and temperatures. The comparison results indicate that the Apelblat equation is superior to the others. Furthermore, a new method (named the calculation method) for determining the Apelblat equation using only three data points was proposed to solve the problem that there may not be enough solute in the determination of solubility. The log-logistic distribution function was used to further capture the trend of the correlation and to make better quantitative comparison between predicted data and the experimental ones for the Apelblat equation determined by different methods (fitting method or calculation method). It is found that the proposed calculation method not only greatly reduces the number of test data points, but also has satisfactory prediction accuracy.