• Title/Summary/Keyword: Melting curve

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Long-Term Runoff Simulation in Consideration of Snow Pack and Snow Melt (적설 및 융설의 영향을 고려한 장기유출 모의)

  • Kim, Dae Geun;Jeong, Jae Ung;Park, Jae Hyun;Park, Chang Geun
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.3
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    • pp.265-272
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    • 2007
  • This study uses the SWAT model to analyze the characteristics of long-term runoff at the Ssang-cheon Basin located in the city of Sokcho, which is located in the province of Gangwon. The study considers the effect of snow packing and snow melting in a runoff simulation. In this simulation, the study examines the need to introduce a snow pack and snow melt model to evaluate the water resources of the mountainous region of the Gangwon province. The findings of this study indicate that the runoff hydrograph that was produced approximates the true measured flow when the effect of the snow pack and snow melt are considered, compared to when they are not factored in. The analysis of the flow duration curve indicates that the stream flow largely increases when the effect of the snow pack and snow melt are considered. The wet stream flow was shown to increase by nearly 3% due to the melting effect, while the normal stream flow, low stream flow and drought stream flow were shown to increase by slightly more than 10%. Specifically, it was found that as the stream flow decreases, the effect of the snow pack and snow melt on the stream flow increases.

Peptide Nucleic Acid Probe-Based Analysis as a New Detection Method for Clarithromycin Resistance in Helicobacter pylori

  • Jung, Da Hyun;Kim, Jie-Hyun;Jeong, Su Jin;Park, Soon Young;Kang, Il-Mo;Lee, Kyoung Hwa;Song, Young Goo
    • Gut and Liver
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    • v.12 no.6
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    • pp.641-647
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    • 2018
  • Background/Aims: Helicobacter pylori eradication rates are decreasing because of increases in clarithromycin resistance. Thus, finding an easy and accurate method of detecting clarithromycin resistance is important. Methods: We evaluated 70 H. pylori isolates from Korean patients. Dual-labeled peptide nucleic acid (PNA) probes were designed to detect resistance associated with point mutations in 23S ribosomal ribonucleic acid gene domain V (A2142G, A2143G, and T2182C). Data were analyzed by probe-based fluorescence melting curve analysis based on probe-target dissociation temperatures and compared with Sanger sequencing. Results: Among 70 H. pylori isolates, 0, 16, and 58 isolates contained A2142G, A2143G, and T2182C mutations, respectively. PNA probe-based analysis exhibited 100.0% positive predictive values for A2142G and A2143G and a 98.3% positive predictive value for T2182C. PNA probe-based analysis results correlated with 98.6% of Sanger sequencing results (${\kappa}$-value=0.990; standard error, 0.010). Conclusions: H. pylori clarithromycin resistance can be easily and accurately assessed by dual-labeled PNA probe-based melting curve analysis if probes are used based on the appropriate resistance-related mutations. This method is fast, simple, accurate, and adaptable for clinical samples. It may help clinicians choose a precise eradication regimen.

Purification of pivalic acid : its evaluation as a temperature standard reference material (온도표준 기준 물질로의 개발을 위한 pivalic acid의 고순도 정제)

  • M. Karthikeyan;M. B. Koss;M. E. Glicksman;Kee-Kahb Koo
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.1
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    • pp.151-162
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    • 1997
  • The suitability of pivalic acid was confirmed as a secondary temperature standard material. Triple-point cells of pivalic acid were obtained by using combined process of vacuum distillation with zone refining. A detailed description of the purification process was given. The melting curves were used as criteria for determination of purities of pivalic acid. Triple points of these cells with purity of 99.9997% were measured to be $35.956\pm 0.003^{\circ}C$ by using the melting plateau curves. Thus the triple point cells of pivalic acid appear to be able to use for the calibration of thermistor thermometers with moderate precision.

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A Study on the Sheet Separation Reduction of Stainless Steel using Hollow Spot Electrode Tip for Resistance Spot Welding (가공 전극 팁을 이용한 스테인레스 저항 용접 외판 변형 저감)

  • Huh, Dong-Woon;Rhee, Se-Hun
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.20 no.6
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    • pp.679-684
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    • 2011
  • Recently, STS 301L joints of side panels to frames for stainless steel rolling stock have been made out by using existing welding methods including Resistance Spot Welding, Laser Welding and Arc Welding. Most of the processes were jointed by spot welding because it is faster at welding and comparatively less expensive for investment in welding facilities than other methods. During spot welding, however, indentation of the metal surface was made due to pressure and melting property of welding. Moreover, since the melting metal was forced to periphery of the plate as indentation was made, sheet separation, which cracked apart between jointed sheets, was carried out. A slight deformation which resulted from sheet separation deteriorated the emotional quality of railway vehicles. This paper suggests that by processing conventional spot electrode tip appropriately, melting metal is able to push up around the processed part (Hollow Spot Electrode Tip) and prevent from being dislodged from first place to periphery. Consequently, sheet separation is remarkably decreased. Also, the emotional quality of appearance of stainless steel rolling stock is enhanced considerably.

Effect the I-T curve and electrical characteristic of fuse elements by plated tin thickness (주석 도금 두께에 따른 퓨즈 가용체의 I-T 커브 및 전기적 특성의 영향)

  • Jin, Sang-Jun;Kim, Eun-Min;Youn, Jae-Seo;Lee, Ye-Ji;Noh, Seong-Yeo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.6
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    • pp.80-87
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    • 2018
  • In recent years, due to the spread of various renewable energy power sources and the pursuit of high efficiency and low-power consumption, not only trends in the electric power industry but also the consumption, control methods, and characteristics are diversified. However, in this diversified electric power industry, the fuse (which is the core part responsible for safety) has not developed significantly in classical operation mode, and thus, fires continue to occur. In this paper, the effects of low melting-point metal plating and high melting-point metal plating on operating characteristics and IT curve movement of the fuse are investigated in a cartridge fuse, which is a classic fuse manufacturing method. The effects of plating on the thickness of the fuse are investigated, and various operating characteristics of the fuse are implemented. In addition, it is suggested that the plating of the low melting-point metal moves the rated current line of the fuse to a low rating, and moves operating characteristics to characteristics of delay operation. It is possible to design various operating characteristics using this characteristic.

Novel non-invasive molecular identification method for two tree frogs, Dryophytes suweonensis and Dryophytes japonicus, based on high resolution melting(HRM) analysis

  • Nakyung Yoo;Keun-Yong Kim;Jung Soo Heo;Ju-Duk Yoon;Keun-Sik Kim
    • Korean Journal of Environmental Biology
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    • v.40 no.2
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    • pp.199-205
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    • 2022
  • Two tree frogs, Dryophytes suweonensis and Dryophytes japonicus, inhabiting Korea, are morphologically similar and share the same habitats. Therefore, they are identified mainly through their calls, especially for males. Dryophytes suweonensis is registered as an endangered (IUCN: EN grade) and protected species in South Korea. Thus, it is necessary to develop a method to rapidly identify and discriminate the two species and establish efficient protection and restoration plans. We identified significant genetic variation between them by sequencing a maternally-inherited mitochondrial 12S ribosomal DNA region. Based on the sequence data, we designed a pair of primers containing 7bp differences for high resolution melting(HRM) analysis to rapidly and accurately characterize their genotypes. The HRM analysis using genomic DNA showed that the melting peak for D. suweonensis was 76.4±0.06℃, whereas that of D. japonicus was 75.0±0.05℃. The differential melt curve plot further showed a distinct difference between them. We also carried out a pilot test for the application of HRM analysis based on immersing D. suweonensis in distilled water for 30 min to generate artificial environmental DNA(eDNA). The results showed 1.10-1.31℃ differences in the melting peaks between the two tree frog samples. Therefore, this HRM analysis is rapid and accurate in identifying two tree frogs not only using their genomic DNA but also using highly non-invasive eDNA.

A study on the heat transfer characteristics during outward melting process of ice in a vertical cylinder (수직원통형 빙축열조내 외향용융과정시 열전달특성에 관한 연구 -작동유체의 유동방향 및 축열조 형상비에 따른 열성능 비교-)

  • Kim, D.H.;Kim, D.C.;Kim, I.G.;Kim, Y.K.;Yim, C.S.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.9 no.2
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    • pp.171-179
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    • 1997
  • During the day time in summer, peak of air conditing load, and electric power management system lies under overloaded condition. The reason is the enlarged peak load value of electric power caused by increased air-cooling load in summer. To prevent load concentration during day time and overloaded condition of power management system, some energy storage methods are suggested. One of these methods is ice storage system. Water has some good properties as P.C.M.(Phase Chang Material) : Its melting point is the range of required operation temperature. It has large specific latent heat and is chemically stable compared to other organic or inorganic substances. It is cheap and easy to treat. This study represents experimental results of heat transfer characteristics of P.C.M. under the outward melting process in a vertical cylinder. We experimented with twelve combinations of conditions, i.e., three different inlet temperatures($7^{\circ}C,\;4^{\circ}C\;and\;1^{\circ}C$), two working fluid directions(upward and downward), and two aspect ratios, H/R(4 and 2). At the inlet temperature of $7^{\circ}C$ and $4^{\circ}C$, there was temperature stagnation region where the temperature of P.C.M. remains constant at $4^{\circ}C$ regardless of aspect ratio and direction of working fluid. This temperature stagnation occurs as the water, at its maximum density, flows down to the lower region. The phase change interface formed bell-shaped curve as the melting process continued. With a new set of conditions(4H/R, inlet temperature $4^{\circ}C$ and $1^{\circ}C$, downward/upwerd inlet direction), the movement of phase change interface was faster when the working flued inlet direction was downward. With the same set of conditions, melting rate and total melting energy were larger when the working fluid inlet direction was downward. The results were reversed when the other sets of conditions were applied.

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Computer Curve-Fitting of Crystallization Peak of Amorphous $Pb(Fe_{2/3}W_{1/3})O_3$ Ceramics (비정질 $Pb(Fe_{2/3}W_{1/3})O_3$ 결정화 피크의 컴퓨터 합성)

  • 김남경
    • Journal of the Korean Ceramic Society
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    • v.31 no.10
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    • pp.1202-1208
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    • 1994
  • On heating amorphous Pb(Fe2/3W1/3)O3 ceramics, glass transition occurred at 35$0^{\circ}C$, crystallization at 394~420, 485~550 and 540~571$^{\circ}C$ (depending on the heating rates of 2.5~8$0^{\circ}C$/min), phase transformation at 680 and 73$0^{\circ}C$, and melting at 935$^{\circ}C$. A curve-fitting computer program was made using FORTRAN, based on a kinetic equation. Reconstructed thermograms (using the program) turned out to be very close to the measured DSC results for all the heating rates, with average deviation of only 4% at maximum.

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Evaluation on Spalling Properties of Ultra High Strength Concrete with Melting and Vaporization of Fiber (유기섬유의 용융 및 기화에 따른 초고강도 콘크리트의 폭렬 특성 평가)

  • Kim, Gyu-Yong;Choe, Gyeong-Cheol;Lee, Joo-Ha;Lee, Seung-Hoon;Lee, Tae-Gyu
    • Journal of the Korea Concrete Institute
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    • v.24 no.2
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    • pp.173-183
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    • 2012
  • Recently, experimental studies to prevent explosive spalling based on spalling mechanism and addition of Polypropylene fiber in high strength concrete (HSC) are performed actively. However, with respect to ultra high strength concrete (UHSC), its compact internal structure is more difficult release vapor pressure at rapid rising temperature compared to HSC. Therefore, in this study, an experiment was conducted to evaluate spalling properties of UHSC using ${\Box}$ $100mm{\times}100{\times}H200mm$ rectangular specimen according to ISO-834 standard fire curve. With respect melting point of fiber, three fiber types of Polyethylene, Polypropylene, and Nylon fibers with melting temperature of $110^{\circ}C$, $165^{\circ}C$, and $225^{\circ}C$, respectively, were considered. Mixed fiber of 0.15% and 0.25% of concrete volume was used to consider spalling properties based on water vapor pressure release. Then, TGDTA test on fiber and FEM analysis were performed. The results showed that it is difficult to prevent initial spalling without loss of fiber mass even if fiber melting temperature is low. Also, in preventing thermal spalling, fiber that melts to rapidly create porosity within 10 minutes of fire is more effective than that of low melting temperature property of fiber.

Identification of Brucella melitensis isolates originating from Mongolia and diagnostic real-time PCR evaluation using a specific SNP (몽골 유래 Brucella melitensis 동정 및 특이 SNP를 이용한 real-time PCR법에 의한 진단 평가)

  • Kang, Sung-Il;Kim, Ji-Yeon;Kim, Suk Mi;Lee, Jin Ju;Sung, So-Ra;Kim, Yeon-Hee;Jung, Suk Chan;Her, Moon
    • Korean Journal of Veterinary Research
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    • v.55 no.2
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    • pp.105-110
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    • 2015
  • A real-time PCR assay using hybridization probe (HybProbe) has been developed to detect Brucella (B.) melitensis strains. The primer and HybProbe sets were designed based on the gap gene of chromosome I with a specific single nucleotide polymorphism of B. melitensis. Specificity of the assay was confirmed by comparison to reference Brucella species and other related strains. In the melting curve analysis, B. melitensis generated a peak at $67^{\circ}C$ unlike those for other Brucella species observed at $61^{\circ}C$. Sensitivity of the assay for B. melitensis ranged from 20 ng to 200 fg of genomic DNA. The ability to identify 94 Mongolian B. melitensis isolates using the real-time PCR assay was identical to that of classical biotyping methods and differential multiplex PCR. These data showed that this new molecular technique is a simple and quick method for detecting B. melitensis, which will be important for the control and prevention of brucellosis.