• 한국생활과학회지
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• 제14권5호
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• pp.795-803
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• 2005

This paper discusses the late nineteenth-century Korean intellectuals' understanding of Japanese customs on the basis of Ki-Su Kim(金綺秀)'s records. Ki-Su Kim was conservative on his inspection and observation trip to Japan Even though he was loyal to Chinese philosophy, he expressed his great interest when he was reluctant to see the Western technology flowered in Japan because it is not mentioned in Confucianism(朱子學). However, he, like mordern scholars in the later period of the Chosun dynasty, took an objective view of the military matters, such as the military training of soldiers, weapons, and others. On the one hand, he appreciated the western garment in that it, fitting the human body perfectly, makes people comfortable. In the later period of the Chosun dynasty, the Koreans had a sense of their superiority to the Japanese and held them in contempt, which had been rooted in the Japanese invasion of Chosun in the year of Imjin(AD 1592). Even around AD 1870, the Koreans regarded the Japanese as a barbarian or a savage, even though the Japanese had attempted to modernize their country with the introduction of the Western civilization since the renovation of Meizi(明治).

• 비교문화연구
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• 제19권
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• pp.271-293
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• 2010

Japanese Give and Receive Verbs are divided into "YARU", "MORAU" and "KURERU". These are influenced by the subject, speaker's viewpoint and meaning. Three verbs are used in a different way depending on who is the giver and who is the taker. I analyze "YARU" and "KURERU" Verbs used in TOUSEISYOUSEIKATAGI. It focus on politeness, gender, and meaning when combined with 'TE'. As an expression of politeness, 'Yaru' is to give to a person of lower social status or an animal or plant. 'Ageru' is to give to an equal ora person of lower social status nowadays. However, 'Ageru' which is treated as elegance of the language remained expression of respect, 'Yaru' is used when the receiver is a person of lower social status and equal social status in TOUSEISYOUSEIKATAGI. 'Kureru' is used when the receiver is a person of lower social status and equal social status, 'kudasaru' is used when a person of higher social status gives the speaker something in TOUSEISYOUSEIKATAGI. Women speakers use 'oyarinasai' 'oyariyo' 'ageru' 'okureru' and men speakers use 'yaru' 'kureru'. Speech patterns peculiar to men are 'kuretamae' 'kurenka'. If the verbs are joined to "TE", they obtain abstract meaning as well as a movement of things. They express some modality for action of the preceeding verbs. The modality has the following meanings ; good will, goodness, benefits, kindness, hopeness, expectation, disadvantage, injury, ill will and sarcasm. In addition, 'TE YARU' expresses the speaker's strong will, 'TE KURERU' expresses the speaker's request.

• Journal of Microbiology and Biotechnology
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• 제29권6호
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• pp.877-886
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• 2019

Brewing with buckwheat as an ingredient has been proven to be successful in several previous studies. However, few studies have focused on the effects of buckwheat on the rutin content and antioxidant activity of beer. In order to develop a lager beer with high rutin content and desirable sensory characteristics, tartary buckwheat malt was used as a brewing adjunct. The results showed that the rutin-degrading enzyme was the key factor affecting the rutin content in the wort and beer. Compared to beer made using the common mashing method, the rutin content in the buckwheat beers produced using an improved mashing method was approximately 60 times higher. The total flavonoid contents in buckwheat beers also depended strongly on the mashing methods, ranging from 530.75 to 1,704.68 mg QE/l. The rutin-rich beers also showed better oxidative stability during forced-aging. Meanwhile, the buckwheat beers were found to be acceptable in terms of the main quality attributes, flavor, and taste.

• Preventive Nutrition and Food Science
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• 제18권4호
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• pp.273-279
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• 2013

Protease widely exists in the digestive tract of animals and humans, playing a very important role in protein digestion and absorption. In this study, a high protease-producing strain Planomicrobium sp. L-2 was isolated and identified from the digestive tract of Octopus variabilis. The strain was identified by physiological and biochemical experiments and 16S rDNA sequences analysis. A protease was obtained from the strain Planomicrobium sp. L-2 through ammonium sulfate precipitation, dialysis and enrichment, DEAE-Sephadex A50 anion-exchange chromatography, and Sephadex G-100 gel chromatography. The molecular weight and properties of the protease were characterized, including optimum temperature and pH, thermal stability, protease inhibitions and metal ions. According to our results, the protease from Planomicrobium sp. L-2 strain designated as F1-1 was obtained by three-step separation and purification from crude enzyme. The molecular weight of the protease was 61.4 kDa and its optimum temperature was $40^{\circ}C$. The protease F1-1 showed a broad pH profile for casein hydrolysis between 5.0~11.0. No residual activity was observed after incubation for 40 min at $60^{\circ}C$ and 60 min at $50^{\circ}C$. F1-1 protease was inhibited by $Mn^{2+}$, $Hg^{2+}$, $Pb^{2+}$, $Zn^{2+}$, and $Cu^{2+}$ ions, as well as PMSF, indicating that the protease F1-1 was a serine protease. Additionally, research basis provided by this study could be considered for industrial application of octopus intestinal proteases.

• 비교문화연구
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• 제29권
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• pp.391-411
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• 2012

Request expressions can be defined as expressions that demand or ask the other person to do certain movements. There are direct request expressions that ask the other person to do certain movements directly and indirect request expressions that ask the other person to do certain movements by describing the speaker's condition. The study analyzed gender and hierarchy of speakers and listeners who used 'tekure' and 'tekudasai' in dialog examples of the Meiji Period and the Taisho Period. In those periods, the modern Tokyo dialect was formed and established. "Toseishoseikatagi"in Meiji 10s,"Ukigumo""Natsukodachi""Tajotakon"in Meiji 20s,"Hakai""Botchan"in Meiji 30s,"Huton""Inakakyoshi" in Meiji 40s and "Aruonna"in the Taisho Period were analyzed for the study. 'kure' was used more by male speakers than female speakers. Examples by female speakers were shown on the novels after Meji 30s. In case of male speakers, they often used it to listeners with an equitable relationship at "Toseishoseikatagi"in Meiji 10s but they often used it to younger listeners at "Hakai"in Meiji 30s. 'okure' was used more by female speakers than male speakers. Listeners were varied from older ones to younger ones. In case of female speakers, 'okure' was used more often at "Aruonna"in the Taisho Period than the other novels. In case of male speakers, 'okure' was used only at "Ukigumo""Natsukodachi"and "Hakai". 'Okurenasai' was used outstandingly by female speakers on the form of 'okun_'. In case of 'kudasai', female speakers used it more than male speakers at "Toseishoseikatagi" and "Aruonna"but male speakers used it more than female speakers at "Tajotakon"and "Hakai". Listeners were varied from older ones to younger ones. 'o~kudasai' was not shown until Meiji 20s but shown after Meiji 30s among the analyzed novels. According to gender, it was used a little bit more often by female speakers than male speakers. According to hierarchy, listeners were usually older than speakers. 'o~nasatekudasai' was used more often by male speakers than female speakers. Listeners were also usually older than speakers.

• Animal Bioscience
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• 제37권8호
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• pp.1317-1332
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• 2024

Objective: Rare study of the non-coding and regulatory regions of the genome limits our ability to decode the mechanisms of fatty liver hemorrhage syndrome (FLHS) in chickens. Methods: Herein, we constructed the high-fat diet-induced FLHS chicken model to investigate the genome-wide active enhancers and transcriptome by H3K27ac target chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-Seq) profiles of normal and FLHS liver tissues. Concurrently, an integrative analysis combining ChIP-seq with RNA-Seq and a comparative analysis with chicken FLHS, rat non-alcoholic fatty liver disease (NAFLD) and human NAFLD at the transcriptome level revealed the enhancer and super enhancer target genes and conservative genes involved in metabolic processes. Results: In total, 56 and 199 peak-genes were identified in upregulated peak-genes positively regulated by H3K27ac (Cor (peak-gene correlation) ≥0.5 and log2(FoldChange) ≥1) (PP) and downregulated peak-genes positively regulated by H3K27ac (Cor (peak-gene correlation) ≥0.5 and log2(FoldChange)≤-1) (PN), respectively; then we screened key regulatory targets mainly distributing in lipid metabolism (PCK1, APOA4, APOA1, INHBE) and apoptosis (KIT, NTRK2) together with MAPK and PPAR signaling pathway in FLHS. Intriguingly, PCK1 was also significantly covered in up-regulated super-enhancers (SEs), which further implied the vital role of PCK1 during the development of FLHS. Conclusion: Together, our studies have identified potential therapeutic biomarkers of PCK1 and elucidated novel insights into the pathogenesis of FLHS, especially for the epigenetic perspective.

• 생명과학회지
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• 제23권12호
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• pp.1486-1494
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• 2013

Octopus vulgaris의 장관으로부터 단백질 가수분해력과 활성을 측정함으로서 높은 단백질분해효소 생성능을 가진 균을 분리하여 동정하였다. 균이 생성한 단백질분해효소는 황산암모늄침전, cellulose CM-52 양이온 교환 크로마토그래피, DEAE-Sephadex A50 음이온 교환 크로마토그래피의 3단계를 통해 정제하였다. 장관으로부터 분리한 균중 가장 높은 단백질분해효소 생성능을 가진 균은 Bacillus sp. QDV-3로 나타났으며 이균을 분리한 후 표현형 분석, 생화학적 특성, 16S rRNA 유전자염기서열분석을 통해 Bacteria역, Firmicutes문, Bacilli강, Bacillales목, Bacillaceae과, Bacillus속으로 Bacillus flexus와 99.2%의 유사성을 보이는 것으로 확인하였다. 균이 생성한 단백질 분해효소를 QDV-E로 지정하였으며 61.6 kDa의 분자량을 나타내었다. 이 효소는 pH 9.0~9.5에서 활성을 나타내었고 최적온도는 $40^{\circ}C$였으며 $50^{\circ}C$에서는 60분간 96% 이상의 활성을 보유하였다. Phenyl methyl sulfonyl fluoride (PMSF)에 의하여 활성이 억제 되었으므로 세린 알칼리성 단백 분해 효소인 것으로 결론지었다. 금속이온인 $Mn^{2+}$ 와 $Mg^{2+}$에 의하여 효소활성 상승효과를 보였으며 $Ba^{2+}$, $Zn^{2+}$, 그리고 $Cu^{2+}$에 의하여 활성이 억제되었다.