• Animal Systematics, Evolution and Diversity
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• v.11 no.3
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• pp.389-408
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• 1995

The complete larval development of Hemigrapsus penicillatus(De Haan, 1835) from hatching to first crab stage was obtained by culture in the laboratory. Under culture conditions with salinity 33.3% , temperature $25^{\circ}C$, and photophase 14/10 h light/dark, the megalopa and the first crab instar were attained in minimum of 18 and 29 days after hatching respectively. Five zoeal stages and a megalopal stage are described and illustrated in detail. Morphological characters of H. penicillatus larvae were compared with those of other members within the subfamily Varuninae. Morphological differences among H. penicillatus, H. sanguineus and H. sinensis in megalopal stage are tabulated.

• The Korean Journal of Zoology
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• v.33 no.2
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• pp.200-216
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• 1990

The complete larval development of Scopimera bitympana Shen was descdbed and mustrated from the larvae reared in the lahoratory. S bItympana had five, or occasionally six, zoeal and one megalopal stages. At $25^{\circ}C$, the megalopa and the first crab instar were attained in 24 and 38 days (31 and 48 days in six zoeal series) after hatching respectively. S.bitympana zoeae can be distinguished from other described zoeae in the genus by the toothed carapace spines and the telson with a dorsal and two ventral spines. Megalopa of this species can be distinguished from other ScopimeTa spedes by the feature of carapace. Other minor morphological features of S. bitympana larvae are compared to the previous descripdons of larvae of the genus and the morphological differences are briefly discussed.

• Journal of Aquaculture
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• v.6 no.4
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• pp.255-271
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• 1993

For the development of seedling production techniques of the freshwater crab Eriocheir japonicus, the effects of salinity and temperature on the growth of larvae of the crab were studied. Embryos hatched out as zoea larvae were measured 0.421 mm in average carapace length. Five zoea stages needed 16-26 days for metamorphosis from zoea to megalopa at $22^{\circ}C$ and $24.5\%o$. The average carapace length of the 5th zoea was 1.16 mm and that of the megalopa larvae was 1.89 mm. Each zoea stage could be identified based on both the number of plumosed seatae on the exopodite of maxilliped, and the number of spines on the posteroinner margin of tel son and also based on the rudimentary pleopods appearence. Zoea larvae fed rotifers and Artemia nauplii were healthy and metamorphosing rate from zoea to megalopa was $80\~90\%\;at\;22\~26^{\circ}C\;and\;17.5\~31.5\%o$. The relationship between larval period (Y in days) and water temperature $(X\;in\;^{\circ}C)$ is expressed as Log Y = 3.8604-1.91735 LogX. Water temperature and salinity ranges for better survival and metamorphosis of the larvae were $ 22^{\circ}C\~26^{\circ}C\;(optimum\;at\; 26^{\circ}C)\;and\;17.5\%o\~31.5\%o\;(optimum\;at\;24.5\%)$, respectively. The duration of larval stages tend to longer as salinity levels deviated from optimum particulaly at lower end. All zoea larvae did not survive in freshwater.

• Animal Systematics, Evolution and Diversity
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• v.5 no.2
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• pp.121-137
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• 1989

The larval stages of Scopimera longidactyla reared in the laboratory are described and illustrated in detail. The larval development consists of five zoeal and a megalopal stages. At 25$^{\circ}C$, the megalopa and the first crab instar were attained in 21 and 31 days after hatching, respectively. The larvae of Scopmera can be distinguished from those of other genera in the Scopimerinae and other sumbfamilies in the Ocypodidae by the relativelength of rostral carapace spines to carapace. The larvae of S. longidactyla are similar in morphology to those of S. globosa but distingished by the differences in larval , size, and appendage setation.

• The Korean Journal of Zoology
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• v.33 no.4
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• pp.411-427
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• 1990

The complete larval development of Enocheir japonicus De Haan reared in the laboratory is described and illustrated. E. japonicus passes through 5 zoeal stages before metamorphosis to the megalopal stage. The megalopa and crab I inStar are attained in 17 and 27 days after hatching, respectively. The culture was carried out under a photoperiod of 14 h light and 10 h dark and a constant temperature of $25^{\circ}C$. Morphological charaderistics of each larval stage of this species are compared with those of E. japonicus from Japan. Morphological features between zoea I of the Varuninae are briefly discussed.

• Journal of fish pathology
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• v.17 no.1
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• pp.67-74
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• 2004

This study investigates the influence of waterborne ammonia and nitrite on the zoea and megalopa stage larvae of tiger crab, Orithyia sinica under laboratory condition, focusing on the effects on survival and growth as deleterious responses of toxicant. Survival rate of zoea stage larvae exposed to control levels, and to 5, 10, 20 and 50 mg/L total ammonia-N, using a continuous flow system for 20 days was 80, 77, 45, 40 and 37%, respectively. Growth rate of zoea stage larvae exposed to 20 and 50 mg/L total ammonia was significantly lower than in controls after 20 days (P< 0.05). Survival rate and growth rate of megalopa stage larvae exposed to ammonia also decreased at greater than 10 and 50 mg/L, respectively. In the nitrite exposure experiment with zoea and megalopa stage larvae of tiger crab, survival rate was decreased in a concentration and exposure period-dependent way. The growth rate of zoea and megalopa stage larvae of tiger crab exposed to nitrite decreased at greater than 150 mg/L nitrite concentration.

• Fisheries and Aquatic Sciences
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• v.27 no.3
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• pp.180-194
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• 2024

Mass mortalities of mud crab Scylla spp. larvae due to pathogenic Vibrio spp. outbreaks have frequently occurred in hatcheries. To overcome this problem, probiotics containing Bacillus subtilis bacteria are applied to inhibit pathogenic ones. We tested different doses of probiotic-containing B. subtilis (10⁸ CFU/g) on the Scylla tranquebarica larvae and investigated the microbiota population, including Vibrio. Water quality, larvae development, and crablet production were also monitored. The recently hatched larvae were grown in twelve conical fiber tanks filled with 200 L sterile seawater, with a salinity of 30 ppt at a stocking density of 80 ind/L. Four different doses of probiotics were applied in the larvae rearing, namely, A = 2.5 mg/L, B = 5 mg/L, C = 7.5 mg/L, and D = 0 mg/L, with three replicates. Next-generation sequencing analysis was used to obtain the abundance of microbes in the whole body of megalopa and the water media for larvae rearing after applying probiotics. Sixteen Raw Deoxyribonucleic Acid samples (eight from a whole body of megalopa extraction from four treatments of probiotics defined as A, B, C, D, and eight from water media extraction from four treatments of probiotic defined as E, F, G, H) were prepared. Then, they were sent to the Genetics Science Laboratory for NGS analysis. Ammonia, nitrite, total organic matter (TOM), larvae, and crablet production were monitored. Based on the Next-generation sequencing analysis data, the Vibrio spp. decreased significantly (p < 0.05) than control test (D) in megalopa-applied probiotics at the doses of 2.5 mg/L (A) and 7.5 mg/L (C) and in the water media for megalopa rearing treated with probiotics at the dosage of 5.0 mg/L (F). Ammonia in the zoea stage in B treatment and TOM in the zoea and megalopa stage in B and C treatments were decreased significantly (p < 0.05). It impacts the higher number of zoea survival in treatments B and C. Finally, it results in a significantly high crablet production in treatments B and C. Therefore, the dosage of 5 mg/L to 7.5 mg/L improves crablet S. tranquebarica production significantly.

• Animal Systematics, Evolution and Diversity
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• v.9 no.2
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• pp.69-86
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• 1993

The larval stages of Hemigrapsus sanguineus were reared in the laboratory and described with illustrative figures. Five zoeal and one megalopal stages were found in the complete larval development. At 25^{\circ}C.$, the megalopa and the first crab instar were attained in 18 and 31 days after hatching, respectively. The first zoeal larva of H. sanguineus showed the characteristics ~ h i c h coincide with those of the same genus by bearing lateral spines on carapace, B-types of telson and antenna, 1 +5 setae on the endopod of maxillule, and 2 + 2 setae on the endopod of maxilla. Morphological features of H. sanguineus larvae were compared to the previous descriptions of larvae of the same genus and morphological differences among them were discussed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.2
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• pp.162-166
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• 2011

The distribution and occurrence of Charybdis japonica larvae were investigated off Yeonpyong-do, Korea, in the Yellow Sea. C. japonica larvae were collected monthly at 15 stations from early June to late October in 2006 and 2007. At each station, a Bongo net with 303 and $505{\mu}m$ mesh was deployed once with a double oblique tow. No larvae were caught in June, in both years. Zoea I was predominant in late July in 2006 and early August in 2007, whereas Zoea I accounted for 84% of all larvae collected and no larval stages later than Zoea III were sampled. Megalopa were the most abundant larval stage at all stations in late August in both years. The timing of larval hatching of C. japonica may be related to that of phytoplankton blooms in the study area. The finding that Zoea I and Megalopa were predominant in the study are may indicate that C. charybdis larvae are carried by advection.

• The Korean Journal of Zoology
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• v.38 no.4
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• pp.465-482
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• 1995

The 7 zoeal and 1 megalopal stages of Cha~bdi8 bimaculata (Miers, 1886) are descrihed and iflustrated in detail from larvae reared in lahoratory. Under isboratory conditions of 22$^{\circ}C$ temperature and 33.3~ salinity, the magalopa was attained in 32 days or more after hatching. The larval morphology of the first zoea of C. bimaculata are coincide with those of Charybdis species by hearing lateral spines on carapace, ~type antenna, A-type telson, 1,6 setae on the endoped of maxillule, and 6 setae on the endopod of maxilla. Morphological features of C. bimaculata larvae were compared to the previous descriptions of larvae of the other spedes helonging to the same genus.