• 한국미생물·생명공학회지
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• 제22권5호
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• pp.557-560
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• 1994

The optimum medium composition for the production of L-tryptophan with Klebsiella pnuemoniae pheA tyrA trpE trpR/pSC 101-trp$^{+}$ and the effect of precusors in the optimum medium were studied. The specific growth rate in the optimum medium was almost the same as that in the basal medium, the former showing 1.01 and the latter 1.07 hr $$^{-1}$, but the produced tryptophan was increased 45% in the optimum medium. The maximum amount of produced tryptophan was 159 mg/l within 14 hours. Tryptophan production was ceased by casamino acid addition over 4 g/l in medium, but cell maSS increased with its addition. Indole and anthranilate as precusors had toxic effect on growth and tryptophan production at experimented concentration range (over 20 mg/l), but L-serine had good effect on tryptophan production, resulting in 175 mg/l tryptophan within 14 hours.

• 터널과지하공간
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• 제20권6호
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• pp.465-474
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• 2010

중규모 터널(연장 1 km 이내) 건설은 최근 매년 30% 이상의 증가율을 보이고 있으나 환기 및 방재시설 설치기준은 제정되어 있지 않은 상태라 장대터널을 대상으로 한 설치기준을 따르고 있다. 이에 따라 중규모터널 환기 및 방재시설의 최적화 노력이 요구되고 있다. 본 연구에서는 중규모 터널 내 20 MW 규모의 화재 시 발생하는 화재연 역류거리, 고온 열기류 확산범위, 가시거리 20 m 이하(상류) 구간분포, 임계풍속 확보여부, 대피시간 등을 CFD 분석을 통해 방재팬의 적정 초기 가동시간을 도출하여 방재시스템의 최적운전 방안의 제시를 목적으로 한다.

• 환경영향평가
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• 제21권5호
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• pp.609-615
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• 2012

In recent years, the development of computer technique was possible to the simulation analysis of the structure caused by ground vibration. Generally, finite element method(FEM) has been used in these structural analysis. In this study, it was calculated to the vibration energy as measuring vibration waveform, and estimated about principal stress due to medium characteristics of the ground as processing dynamic analysis by the vibration energy. The results are as follows : Firstly, the principal stress distribution in all mediums was different due to a medium condition, and the principal stress at concrete medium was represented to difference due to physical characteristics. Secondly, the principal stress by time increasing was represented to maximum amplitude within 0.03 second. And also, the principal stress after maximum amplitude was very large at concrete medium, which was considered to be formed compression or tension range at a medium boundary. Thirdly, the variation of principal stress at concrete medium was represented in the order of RC medium, NC=H medium, NC=S medium. It was considered that the vibration energy propagated fast when a medium have a big elasticity and density.

• Journal of Microbiology and Biotechnology
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• 제2권2호
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• pp.141-151
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• 1992

Physiological changes of the murine hybridoma cell line $S3H5/\gamma{2bA2}$ during adaptation to RPMI 1640 medium with 1%(v/v) fetal bovine serum were characterized in terms of cell growth, antibody production, morphology, and metabolic quotients. Cells adapted to 1% serum medium in T-flasks became sensitive to shear induced by mechanical agitation and required at least 5% serum in the medium or spent medium for cell growth in spinner flasks, while cells adapted to 10% serum medium in T-flasks could grow in 1% serum medium in spinner flasks. Consequently, long-term adaptation to low serum media may not give the expected growth enhancement. After adaptation to 1% serum medium, changes in cell morphology were observed. The cells in 10% serum medium were uniform and circular, while cells in 1% medium were irregularly shaped. The DNA contents, which were measured by flow cytometry, were almost constant among the cells in the range of 1% to 10%. Further, no significant changes in energy metabolism and specific monoclonal antibody production rate were observed among these cells.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.73-80
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• 2023

일반적으로 여러 다양한 지방산들은 넓은 범위의 미생물들에 대해 항균활성을 나타내는 것으로 알려져 있다. 그러나 지방산의 구조에는 다양한 구조적 차이가 있음에도 불구하고 지방산의 구조적 차이에 따른 항균활성의 변화에 대해서는 많이 알려져 있지 않다. 본 연구는 중쇄지방산들을 대상으로 카복실기의 숫자와 위치에 따른 항균활성의 차이를 잘 알려져 있는 식중독균들을 대상으로 조사하였다. 전반적으로 단일카복실기를 갖는 중쇄 지방산이 이중카복실기를 갖는 중쇄지방산에 비해 더 강한 항균활성을 나타내었지만 두 종류의 중쇄지방산 모두 일반 항생제들과 함께 사용될 경우 매우 높은 시너지 효과를 나타낸다는 사실이 추가적으로 확인되었다.

• Journal of Microbiology and Biotechnology
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• 제9권4호
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• pp.443-449
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• 1999

Bacillus subtilis BK-17 which produces a novel protease with fibrinolytic activity was isolated from soybean paste. Bioreactor production of the enzyme was studied in order to optimize fermentation conditions such as medium concentration, pH, agitation speed, and temperature. Under most cultural conditions, enzyme production initially began when the cell growth stopped. The onset of the enzyme production was indicated by rapid increase in both dissolved oxygen (DO) and pH. Two- to three-times more concentrated medium than the flask optimum medium yielded higher enzyme production in the bioreactor fermentation. When the medium pH was controlled constant, pH 6.5 exhibited the highest activity in the range of 6.0 to 7.5, but the activity was similar to the case when the pH was initially adjusted to 7.5 and subsequently maintained within a relatively wide range of 6.4 to 7.8. Agitation speed did not affect the enzyme production with an exception of DO reaching zero. Fermentation time was reduced when temperature increased within the range of $25^{\circ}C$ to$37^{\circ}C$. However, the highest activity, along with the slow decrease of the enzymatic activity after reaching the maximum value, was observed at $25^{\circ}C$. By shifting the temperature from $37^{\circ}C$ to $25^{\circ}C$immediately after DO reached the minimum level, the high enzyme production of 1,100 U/ml along with the short fermentation period of 13 h could be obtained.

• Journal of Biosystems Engineering
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• 제2권1호
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• pp.25-32
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• 1977

This experiment was conducted in order to find out the drawing performance of a two-wheel tractor under different levels of the soil moisture and hardness, so as to obtain some basic data for improving their drawing performance. With fairly homogeneous soil, 5 levels of soil moisture contents (8, 13, 17, 20 and 23%) and 3 levels of soil hardness (0 , 2 and 4kg/$cm^2$) were selected for this experiment.The summerized results are as follows ;1. The draft force, on the hard soil (hardness ; 4kg/$cm^2$), had a distinct tendency to decrease with the increasing soil moisture. On the medium soil (hardness ; 2 kg/$cm^2$), and the soft soil (hardness ; 0kg$cm^2$), the draft force showed the highest when the moisture contents were within the range of 16-19%.But the maximum draft force, on the soft soil, was higher than that on the medium soil by 10 %. 2. The driving axle torque increased with increasing soil by 10 %. 3.The values of horizontal distance between the soil reaction point and axle shaft were within the range of 0~10cm , and it had the tendency to increase with the increasing soil moisture. Also, it s value was the largest on the hard soil and the smallest on the soft soil. 4.The tractive efficiency decreased with the increasing soil moisture. On the hard soil, the average value of tractive efficiency was higher than that on the medium soil by 19.0% and that on the soft soil was lower than that on the medium soil. 5.The traction ratio were within the range of 30 ~45%, and their changing tendency with respect to the soil moisture was similar to that in the case of the draft force. 6. The travel resistance ratio tended to increased with increasing soil moisture, and the highest value was found on the soft soil, and the lowest on the hard soil.

• 한국습지학회지
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• 제3권1호
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• pp.75-89
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• 2001

Constructed wetlands were typically cost less to build and operate, and require less energy than standard mechanical treatment technology but they have similar performance to centralized wastewater treatment plants. Therefore, they were constructed especially many in rural areas, where are small villages but not industries. Accordingly, plantless column tests were performed to investigate the possibility on using zeolite as a filter medium of constructed wetland for the wastewater treatment. $COD_{cr}$ removal efficiency was 94.63% at hydraulic load $314L/m^2{\cdot}d$ and filtering hight 100cm filled with a zeolite mixture. This zeolite mixture consisted of 1 : 1 by volume of a zeolite in the diameter range of 0.5 to 1mm to a zeolite in the diameter range of 1 to 3mm. According, hydraulic load $314L/m^2{\cdot}d$ was considered as optimal. Three zeolite mixture were used to determine the optimal mixing ratio by volume of a zeolite(A) in the diameter range of 0.5 to 1mm to a zeolite(B) in the diameter range of 1 to 3mm diameter. 1 : 3, 1 : 1 and only B in A to B by volume were tested at hydraulic load $314L/m^2{\cdot}d$ and filtering hight 100cm. $COD_{cr}$ removal efficiency was more than 89% at mixing ratios of 1 : 3 and 1 : 1 in A to B. Removal efficiency was lower at the column filled with only B. Removal efficiency was better at filter medium filled with mixing ratio 1 : 1 in A to B than with the other mixing ratios. Thus, it was found that the mixture of mixing ratio 1 : 1 in A to B was appropriate for filter medium of constructed wetland. Removal efficiency was higher in down-flow than in up-flow, and $COD_{cr}$ and BOD were removed best in 20cm filter height near feeding area.

• KSBB Journal
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• 제18권1호
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• pp.51-54
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• 2003

이 연구의 목적은 미세조류인 Chlamydomonas reinhardtii을 이용하여 수소 및 유기물의 생산을 위한 배양 조건을 최적화 하는 것이다. 세 가지 종의 균주를 조사하여 C. reinhardtii UTEX 90이 수소 생산성과 유기물 생산성 면에서 가장 우수함을 확인하였다. 최적 이산화탄소 농도 범위는 1-3% 였으며 두 종류의 미세조류 배양에 있어 대표적인 배지 (Bristol's media and TAP media)를 조사한 결과 TAP 배지에서의 세포성장이 Bristol 배지보다 더 우수함을 확인하였다. 3%의 이산화탄소 농도와 TAP 배지에서 균주를 배양하여 최대 수소 생산($0.5\mu$ mol/mg DCW)을 얻었다. 그러나 전체 유기물 생산은 Bristol 배지에서 두 배 이상 생산되었다.

• 대한물리치료과학회지
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• 제31권1호
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• pp.33-45
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• 2024

Background: This study aimed to investigate the effect of elastic band-resistive exercise using audio-visual medium on pain, proprioception, and motor function in adults with chronic neck and shoulder pain. Design: One group pretest-posttest follow-up experimental design. Method: Twenty adult women with neck and shoulder pain voluntarily participated in this study. Elastic band-resistive exercise using audio-visual medium including cervical flexion and extension, shoulder external rotation, and scapular retraction-protraction motions was conducted 5 times a week for 3 weeks. The Numerical Rating Scale, pressure threshold tool, CROM goniometer, and Image J software were used to assess subjective pain level, tenderness threshold (pain), joint position sense error (proprioception), joint range of motion, and postural alignment (motor function), respectively. Result:: The pain intensity and threshold and joint position sense error showed significant decreases after the intervention, whereas the joint range of motion angle revealed significant increases. The postural alignment including forward head posture and rounded shoulder revealed significant improvements after the intervention. Conclusions: Therefore, we suggest that elastic band-resistive exercise through audio-visual medium would be helpful in preventing and managing pain and physical dysfunction in individuals with chronic neck and shoulder pain, and then it would support the development of health management-related online education content.