• Journal of Plant Biotechnology
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• 제34권4호
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• pp.293-298
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• 2007

Robinia pseudoacacia var. umbraculifera, commonly called umbrella black locust were regenerated after co-cultivation of internode segments with Agrobacterium tumefaciens which included yeast cadmium factor 1 (YCF 1) gene. The tolerance to cadmium and lead for plants can be increased by the YCF1 gene expression. Moreover, the recent studies have shown that YCF1 gene transgenic plants increase the accumulation of cadmium and lead into plant vacuoles. The effect of plant growth regulator such as 2,4-dichlorophenoxyacetic acid (2,4-D), ${\alpha}$-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and thidiazuron (TDZ) were studied to evaluate the propagation of plants through internode explants. The efficient induction of multiple adventitious shoots and callus were observed on a medium supplemented with 0.1 mg/L TDZ + 0.2 mg/L BA. To induce shoot elongation and rooting, regenerated shoots were transferred into basal MS medium without any plant growth regulator. Successful Agrobacterium tumefaciens mediated transformation was obtained by 20 min vacuum-infiltration with $50{\mu}M$ acetosyringone on the optimal multiple shoot induction medium with 30 mg/L hygromycin and 300 mg/L cefotaxime. To confirm the integration and expression of transgene, Polymerase Chain Reaction (PCR) and Reverse Transcriptase PCR (RT-PCR) were performed with specific primers. The frequency of transformation was approximately 18.94%. This study can be used to genetic engineering of phytoremediator.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2012년도 춘계학술대회
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• pp.80-82
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• 2012

본 논문에서는 Debye 분산 특성을 가지는 복제 방지용 PUF를 설계하기 위한 전자계 해석 방안을 고려하였다. 공기층과 분산매질(Si)로 구성된 1차원 모델 내에 전파하는 펄스를 모형하기 위해 FDTD법을 이용하였다. 불연속 경계면에 도달한 펄스는 일부 반사되고 일부는 투과되어 빠르게 감쇠되는 것으로 나타났다. 그 결과 FDTD법에 의한 Debye 분산특성을 가지는 1차원 복제방지용 PUF 설계 및 모델링에 적용 가능한 것을 확인하였다.

• KSBB Journal
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• 제14권5호
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• pp.561-565
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• 1999

Optimum conditions for vacuum-drying ad cultivation of yeast cells for the production of active dry yeast were examined. At lower temperature, more drying time was required to dry the yeast pellet to reach the desirable water content(8%). Optimum temperature of vaccum oven and time for drying was 63$^{\circ}C$ and 90 min, respectively. Optimum medium composition for flask culture using cane molasses as the substrate were 0.25% sugar, 0.013% $K_2$HPO$_4$, 0.1% $K_2$HPO$_4$. and 0.125% (NH$_4$)$_2$SO$_4$. Culture temperature $25^{\circ}C$ gave the highest survival rate of dired yeast. After finishing fed-batch culture and the culture was left in the fermentor without adding any sugar or nutrient, survival of the dried yeast harvested from the fermentor increased to 86.0% after 36 hr. It was also observed that the yeast cells with higher budding rates showed lower survival rate.

• 한국정보통신학회논문지
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• 제16권6호
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• pp.1141-1147
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• 2012

본 논문에서는 Debye 분산 특성을 가지는 복제 방지용 PUF를 설계하기 위한 전자계 해석 방안을 고려하였다. 공기층과 유전체 기판 위에 형성된 분산매질(Si)로 구성된 1차원 모델 내에 전파하는 펄스를 모형하기 위해 FDTD법을 이용하였다. 불연속 경계면에 도달한 펄스는 일부 반사되고 일부는 투과되어 빠르게 감쇠되는 것으로 나타났다. 그 결과 FDTD법에 의한 유전체 기판을 고려한 Debye 분산특성을 가지는 1차원 복제방지용 PUF 모델링에 적용 가능한 것을 확인하였다.

• 한국응용과학기술학회지
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• 제17권2호
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• pp.144-148
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• 2000

A modification of the sol-gel method to obtain phase pure superconducting oxides is described. The method starts from organic salts of yttrium, barium and copper, such as acetates, and avoids the sudden and uncontrollable decomposition of the organic fraction which occurs if nitrates are used as starting materials. The aqueous solution obtained with citric acid in an alkaline medium is concentrated under vacuum. The solid so prepared is decomposed at about $300^{\circ}C$ thus giving an oxide precursor containing well dispersed yttrium, barium and copper. Pyrolysis at 850 - $920^{\circ}C$ followed by oxygen annealing gives the superconducting orthorhombic 123 phase. The results of TGA/DTA of the precursor, as well as XRD, electrical and magnetic property measurements on the pyrolysis products are presented and discussed.

• 약학회지
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• 제32권4호
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• pp.234-238
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• 1988

Proliposome of Sudan IV was prepared according to Payne et al. and evaluated for it's size distribution, surface characteristics and conversion to liposome in aqueous medium. The manufacturing procedures for proliposomes involve the coating of phospholipid solution with Sudan IV on the surface of sorbitol particle in rotary vacuum evaporator. As a result, dry, free flowing and stable proliposome was obtained and multi-lamellar liposome of sudan IV was formed spontaneously when water were added to this. Proliposomes were expected as a probable answer for the physical instability of conventional liposomes.

• 한국복합재료학회:학술대회논문집
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• 한국복합재료학회 2003년도 추계학술발표대회 논문집
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• pp.225-230
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• 2003

Domestic small and medium ship companies have lack of leisure boat technologies and especially they have a problem about its low performance because of the overweight of boat hull. So it is necessary to have alternative manufacturing process to improve the mechanical properties of composite material. In this study, a vacuum curing system was developed as an alternative manufacturing process and then changed the fiber volume fractions of GFRP laminates. The properties of GFRP laminates such as void contents, Young's modulus and fracture toughness were determined for various fiber volume fractions.

• 한국초전도ㆍ저온공학회논문지
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• 제15권4호
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• pp.59-62
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• 2013

The conductive link is used as a cooling medium between a cryocooler and magnet in a cryogen-free superconducting magnet system. The low temperature superconducting (LTS) magnet has one solenoidal configuration with a metal former which has a 52 mm room temperature bore. The superconducting coil is installed in the cryostat maintaining high vacuum and cooled by a two-stage cryocooler. In order to maintain the operating temperature of magnet at the designed level, the cold head temperature of the cryocooler must be lower so that heat can be removed from the superconducting coil. Also, temperature difference is occurred between the magnet and cryocooler and its magnitude is dependent upon the contact resistance at the interfacial surface between metals in the conductive link. In the paper, the performance of the LTS magnet is investigated with respect to the conductive link between the magnet former and the cold head of the cryocooler. The effects of the contact pressure and interfacial materials on the temperature distribution along the conductive link are also presented.

• Applied Science and Convergence Technology
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• 제24권6호
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• pp.219-223
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• 2015

Enhancement of the surface hydrophobicity of polydimethylsiloxane (PDMS) thin films deposited on substrates covered with titanium dioxide ($TiO_2$) nanospheres was studied. First, a low-temperature solution-phase method using polyvinylpyrrolidone (PVP) as a surface capping agent and a water/dimethylformamide (DMF) mixture as the reaction medium was used to synthesize monodisperse $TiO_2$ nanospheres. It was possible to easily control hydrolysis rate of the Ti-precursors and the size of the synthesized nanospheres by varying the amount of PVP and the volume ratio of the solvent mixture. Spray coating of the synthesized $TiO_2$ nanospheres under the PDMS film increased the water contact angle of the film surface to $150.3^{\circ}$. This simple treatment can modify the surface morphology at a nanometer scale without any long or complicated nanoprocess; hence, the surface enters the superhydrophobic Cassie-Baxter regime.

• Applied Science and Convergence Technology
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• 제24권6호
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• pp.203-214
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• 2015

We have been developing a new label-free nanobio imaging platform using non-linear optics such as Coherent Anti-Stokes Raman Spectroscopy (CARS) and ion beam techniques based on sputtering and scattering such as Secondary Ion Mass Spectrometry (SIMS) and Medium Energy Ion Scattering Spectroscopy (MEIS), which have been widely used for atomic and molecular level analysis of semiconductors and nanomaterials. To apply techniques developed for semiconductors and nanomaterials for biomedical applications, the convergence of nano-analysis and biology were tried. Our activities on label-free nanobio imaging during the last decade are summarized in this review about non-linear optical 3D imaging, ellipsometric interface imaging, SIMS imaging, and TOF-MEIS nano analysis for cardiovascular tissues, collagen thin films, peptides on microarray, nanoparticles, and cell adhesion studies and finally the present snapshot of nanobio imaging and the future prospect are described.