• Journal of Microbiology and Biotechnology
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• v.27 no.12
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• pp.2112-2118
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• 2017

Leuconostoc mesenteroides is used as a starter to produce high-quality kimchi products. In this study, an efficient and economical cabbage juice medium (CJM) was developed by process optimization of cabbage extraction and pasteurization and by compositional supplementation of various lacking nutrients. The pasteurized cabbage juice was determined to be a good medium candidate to cultivate L. mesenteroides, showing maximal cell numbers ($9.85{\times}10^8CFU/ml$) after 24 h. Addition of sucrose and yeast extract with soy peptone resulted in increment of bacterial cell counts in CJM, showing the supplementing effect of the lacking nutrients. Furthermore, addition of shell powder gave a protective effect on bacterial cells by preventing pH decline and organic acid accumulation in CJM, resulting in a 2-fold increase of bacterial counts. The optimized composition of CJM was 70% cabbage juice diluted with water, 0.5% (w/v) sucrose, 1% (w/v) yeast extract, 1% (w/v) soy peptone, and 1.5% (w/v) ark shell powder. The CJM developed in this study was able to yield a comparable level of bacterial counts with MRS medium and reduced the cost by almost 10-fold.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.37.1-37.1
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• 2019

The faint quasars with M1450 > -24 mag are known to hold the key to the determination of the ultraviolet emissivity for the cosmic reionization. But only a few have been identified so far because of the limitations on the survey data. Here we present the first results of the z ~ 5 faint quasar survey with the Infrared Medium-deep Survey (IMS), which covers ${\sim}100deg^2$ areas in J band to the depths of $J_{AB}$ ~ 23 mag. To improve selection methods, the medium-band follow-up imaging has been carried out using the SED camera for QUasars in Early uNiverse (SQUEAN) on the Otto Struve 2.1 m Telescope. The optical spectra of the candidates were obtained with 8 m class telescopes. We newly discovered 10 quasars with -25 < $M_{1450}$ < -23 at z ~ 5, among which three have been missed in a previous survey using the same optical data over the same area, implying the necessity for improvements in high-redshift faint quasar selection. We derived photometric redshifts from the medium-band data and found that they have high accuracies of ${\langle}{\mid}{\Delta}z{\mid}/(1+z){\rangle}=0.016$. The medium-band-based approach allows us to rule out many of the interlopers that contaminate ${\geq}20%$ of the broadband-selected quasar candidates. These results suggest that the medium-band-based approach is a powerful way to identify z ~ 5 quasars and measure their redshifts at high accuracy (1%-2%). It is also a cost-effective way to understand the contribution of quasars to the cosmic reionization history.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.106-114
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• 2024

Fusarium head blight (FHB), predominantly caused by Fusarium graminearum and F. asiaticum, is a significant fungal disease impacting small-grain cereals. The absence of highly resistant cultivars underscores the need for vigilant FHB surveillance to mitigate its detrimental effects. In 2023, a notable FHB outbreak occurred in the southern region of Korea. We assessed FHB disease severity by quantifying infected spikelets and grains. Isolating fungal pathogens from infected samples often encounters interference from various microorganisms. We developed a cost-effective, selective medium, named BGT (Burkholderia glumae Toxoflavin) medium, utilizing B. glumae, which is primarily known for causing bacterial panicle blight in rice. This medium exhibited selective growth properties, predominantly supporting Fusarium spp., while substantially inhibiting the growth of other fungi. Using the BGT medium, we isolated F. graminearum and F. asiaticum from infected wheat and barley samples across Korea. To further streamline the process, we used a direct PCR approach to amplify the translation elongation factor 1-α (TEF-1α) region without a separate genomic DNA extraction step. Phylogenetic analysis of the TEF-1α region revealed that the majority of the isolates were identified as F. asiaticum. Our results demonstrate that BGT medium is an effective tool for FHB diagnosis and Fusarium strain isolation.

• Nuclear Engineering and Technology
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• v.56 no.5
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• pp.1747-1753
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• 2024

The accurate determination of formation density and the physical properties of rocks is the most critical logging tasks which can be obtained using gamma-ray transport and detection tools. Though the simulation works published so far have considerably improved the knowledge of the parameters that govern the responses of the detectors in these tools, recent studies have found considerable differences between the results of using a conventional model of a homogeneous mixture of formation and fluid and an inhomogeneous fractured medium. It has increased concerns about the importance of the complexity of the model used for the medium in simulation works. In the present study, we have suggested two various models for the flow of the fluid in porous media and fractured rock to be used for logging purposes. For a typical gamma-gamma logging tool containing a ¹³⁷Cs source and two NaI detectors, simulated by using the MCNPX code, a simplified porous (SP) model in which the formation is filled with elongated rectangular cubes loaded with either mineral material or oil was investigated. In this model, the oil directly reaches the top of the medium and the connection between the pores is not guaranteed. In the other model, the medium is a large 3-D matrix of 1 cm³ randomly filled cubes. The designed algorithm to fill the matrix sites is so that this realistic random (RR) model provides the continuum growth of oil flow in various disordered directions and, therefore, fulfills the concerns about modeling the rock textures consist of extremely complex pore structures. For an arbitrary set of oil concentrations and various formation materials, the response of the detectors in the logging tool has been considered as a criterion to assess the effect of modeling for the distribution of pores in the formation on simulation studies. The results show that defining a RR model for describing heterogeneities of a porous medium does not effectively improve the prediction of the responses of logging tools. Taking into account the computational cost of the particle transport in the complex geometries in the Monte Carlo method, the SP model can be satisfactory for gamma-gamma logging purposes.

• Journal of Microbiology and Biotechnology
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• v.29 no.12
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• pp.1982-1992
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• 2019

The alkaliphilic, calcium carbonate precipitating Bacillus sp. strain AK13 can be utilized in concrete for self-repairing. A statistical experimental design was used to develop an economical medium for its mass cultivation and sporulation. Two types of screening experiment were first conducted to identify substrates that promote the growth of the AK13 strain: the first followed a one-factor-at-a-time factorial design and the second a two-level full factorial design. Based on these screening experiments, barley malt powder and mixed grain powder were identified as the substrates that most effectively promoted the growth of the AK13 strain from a range of 21 agricultural products and by-products. A quadratic statistical model was then constructed using a central composite design and the concentration of the two substrates was optimized. The estimated growth and sporulation of Bacillus sp. strain AK13 in the proposed medium were 3.08 ± 0.38 × 10⁸ and 1.25 ± 0.12 × 10⁸ CFU/ml, respectively, which meant that the proposed low-cost medium was approximately 45 times more effective than the commercial medium in terms of the number of cultivatable bacteria per unit price. The spores were then powdered via a spray-drying process to produce a spore powder with a spore count of 2.0 ± 0.7 × 10⁹ CFU/g. The AK13 spore powder was mixed with cement paste, yeast extract, calcium lactate, and water. The yeast extract and calcium lactate generated the highest CFU/ml for AK13 at a 0.4:0.4 ratio compared to 0.4:0.25 (the original ratio of the B4 medium) and 0.4:0.8. Twenty-eight days after the spores were mixed into the mortar, the number of vegetative cells and spores of the AK13 strain had reached 10⁶ CFU/g within the mortar. Cracks in the mortar under 0.29 mm were healed in 14 days. Calcium carbonate precipitation was observed on the crack surface. The mortar containing the spore powder was thus concluded to be effective in terms of healing micro-cracks.

• Journal of Power Electronics
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• v.18 no.2
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• pp.445-455
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• 2018

A novel algorithm is proposed to regulate the neutral point potential in neutral point clamped three-level inverters. Oscillations of the neutral point potential and an unbalanced dc-link voltage cause distortions of the output voltage. Large capacitors, which make the application costly and bulky, are needed to eliminate oscillations. Thus, the algorithm proposed in this paper utilizes the finite-control-set model predictive control and the multistage medium vector to solve these issues. The proposed strategy consists of a two-step prediction and a cost function to evaluate the selected multistage medium vector. Unlike the virtual vector method, the multistage medium vector is a mixture of the virtual vector and the original vector. In addition, its amplitude is variable. The neutral point current generated by it can be used to adjust the neutral point potential. When compared with the virtual vector method, the multistage medium vector contributes to decreasing the regulation time when the modulation index is high. The vectors are rearranged to cope with the variable switching frequency of the model predictive control. Simulation and experimental results verify the validity of the proposed strategy.

• Journal of the Korean Society for Precision Engineering
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• v.32 no.11
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• pp.953-960
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• 2015

The thermoforming process has been widely used to manufacture medium- and large-sized plastic parts because of the relatively low cost and high productivity, as compared with other plastic forming processes. One of current salient issues of thermoforming industries is the reduction of trial and error during the production of the thermoformed product. Hence, there is a significant increasing interest in the thermoforming analysis by the thermoforming industries. The goal of this paper is to investigate a methodology of the three-dimensional thermoforming analysis for medium- and large-sized plastic parts. There is a discussion about methodologies of thermoforming analysis, as well as material modeling, and three-dimensional finite element analysis. Furthermore, there is an examination, through case studies, about the applicability of the proposed methodology concerning the thermoforming analysis.

• Journal of the Korean Society of Systems Engineering
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• v.17 no.1
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• pp.107-113
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• 2021

Plant Configuration Management(CM) is an activity to maintain consistency of design requirements, physical configuration, facility configuration information throughout the life cycle of the plant by systematically managing changes that occur during the plant design and operation process. Conformity between information must be ensured not only in the design stage, but also in the case of design changes in the operation and maintenance stages, and thus a computer system capable of efficiently managing them is required. In particular, in consideration of an application to small and medium-sized domestic plants, a computer system that can support configuration management at a low cost is needed. Accordingly, in this study, a configuration management system has been developed to support the management of plant design information and change procedures in the operation stage of small and medium-sized plants. Here, a case for quantitatively evaluating the effectiveness of the developed system is described.

• Proceedings of the Korea Institute of Convergence Signal Processing
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• 2001.06a
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• pp.57-60
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• 2001

Most power retailers adopt systematic, integral supply chain system to minimize the logistics cost. However, small and medium sized retail shops that don't have a distribution channel, unavoidably spend a big amount of distribution cost. This study is to propose an efficient system to manage the information of trucks by using the portable GPS. This system includes the real-time mapping of truck location, the transmission of delivery information from the truck, and the algorithm concerning the selection of the most cost efficient truck.

• Proceedings of the SAREK Conference
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• 2008.06a
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• pp.214-219
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• 2008

In this study, medium capacity EHP and GHP for air-conditioning a building with a floor area of $1,200\;m^2$ are compared economically. To consider all the factors of initial and operation costs effectively, an annual equal payment method is proposed. For the initial cost, equipment, construction, installation, electric facility, financial subsidy and tax cut are considered. Basic electricity, energy(electricity and gas), space charge, labor, insurance and repair are considered for the operation cost. Under the assumptions made in this study, overall cost of EHP is less than that of GHP. However, different outcome may result if different assumptions are made. This study is useful for performing an economic evaluation of various air-conditioning systems.