• 제목/요약/키워드: Medium composition

검색결과 987건 처리시간 0.028초

Statistical Optimization of the Medium Components for the Production of Protopectinases by Bacillus subtilis

  • Shahbazian, Nafise;Ashtiani, Farzin Zokaee;Bonakdarpour, Babak
    • Food Science and Biotechnology
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    • 제18권2호
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    • pp.442-448
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    • 2009
  • In this study Bacillus subtilis PTCC 1023 was used for the production of protopectinase using soybean based media. The use of isolated soybean protein (ISP) and soybean flour resulted in similar protopectinase production and growth rates. The effect of medium composition on protopectinase production was studied using central composite design (CCD) methodology. The change in the concentration of ISP (1-7%), glucose (0-10%), and phosphate (0.1-0.3 M) was found to affect the protopectinase activity (response variable) after 24 hr of cultivation. In the range studied, ISP and glucose had a negative effect on the response variable, whereas phosphate had a positive effect. A statistically significant interaction was identified between phosphate and ISP, suggesting that correct optimization of medium formulation in this case can only be obtained using factorial design of experiments. Protopectinase activity exceeding 215 U/mL was obtained in a medium containing 4% ISP, 0.3M phosphate, and no added sugar.

Culture Conditions and Growth Characteristics of Bifidobacterium longum

  • KIM, WANG-JUNE;SEONG-KWAN CHA
    • Journal of Microbiology and Biotechnology
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    • 제5권3호
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    • pp.149-153
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    • 1995
  • A simple and low-cost medium was developed for the growth of Bifidobacterium longum KFRI 977. Of three bifidobacterial strains, B. longum KFRI 977 (ATCC 15707) showed the best growth in MRSC broth containing 0.3% oxgall, grew well in partially anaerobic condition, exhibited highest $\beta$-galactosidase activity, and was inhibitory against Clostridium perfringens KFRI 434. Of three developed media, the population of B. longum KFRI 977 was highest (1.9$\times$$10^9$/ml) in ISP based medium. The composition of ISP based medium is ISP (5%), glucose (1%), L-cysteine HCI (0.05%), Trypticase peptone (0.5%), yeast extract (0.5%), $MgSO_4$ (0.05%), Tween-80 (0.1%), and phosphate buffer (pH 7.0). Hydrolysis of ISP by Protease A was unnecessary, and the use of phosphate buffer (pH 7.0) prevented the formation of protein precipitate. Associative culture of B. longum KFRI 977 with Lactobacillus acidophilus KFRI 233 was proven to be deleterous to the growth of B. longum KFRI 977.

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배지종류, 탄소원, 첨가물질 및 치상방법이 복숭아 기내 신초 증식에 미치는 영향 (Influence of Medium Composition, Carbon Source, Addition Agent and Explant Orientation of Shoot Proliferation from Prunus persica in vitro.)

  • 전지혜;정경호;강상조;박소연;예병우
    • 식물조직배양학회지
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    • 제25권2호
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    • pp.99-102
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    • 1998
  • 백미조생, 천홍, 유명 등 3가지 복숭아 품종에 있어서 기내 신초 유기에 적합한 배지는 LP 배지이며, 기내 신초 증식에 효과적인 탄소원은 20 g/L sorbitol 처리와 30 g/L sorbitol 처리였다. 배지내 500mg/L LH를 첨가함으로써 백미조생 품종의 기내 신초 증식율의 향상을 가져올 수 있었으며 신초정부를 제거하고 수평배양하는 것이 기내 신초중식에 효과적이었다.

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실내에서 관수주기, 상토종류, 배수층의 유$\cdot$무에 따른 자생 가는쇠고사리의 생육반응 (Effects of Irrigation Interval, Medium Composition and Drainage on the Growth Response of Korea Native Fern Rumohra aristata at Indoor)

  • 주진희;방광자
    • 한국조경학회지
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    • 제32권6호
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    • pp.103-108
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    • 2005
  • 본 연구는 실내에서의 관수주기, 상토종류, 배수층의 유$\cdot$무에 따른 가는쇠고사리의 생육을 살펴봄으로써 실내조경용 식물소재로 활용 및 관리 방법에 관한 자료를 제공하고자 수행하였다. 관수주기는 주 2회와 주 7회로 처리하였으며 배수층은 각 관수주기 처리별로 500mm 깊이의 마사토로 처리하였다. 상토는 자연토인 마사토:부엽토=1:1와 인공토인 피트모스:버미큘라이트:펄라이트=1:1:1을 사용하였다. 1. 2가지의 상토의 화학적인 특징을 분석한 결과 토양산도, 치환성 양이온함량은 피트모스:버미큘라이트:펄라이트=1:1:1 처리에서, 전기전도도, 유기물함량, 유효인산, 전질소함량, 양이온치환용량은 마사토:부엽토=1:1 처리에서 높은 수준을 보였다. 2. 관수주기에 있어 주 2회 관수처리가 주 7회 관수처리보다 가는쇠고사리의 생육과 실내 적응성이 높아 양치식물이 높은 수분환경을 선호함에도 불구하고 과도한 관수는 바람직하지 않는 것으로 사료된다. 3. 상토에 다른 가는쇠고사리의 생육과 실내 적응성은 마사토:부엽토=1:1 처리가 피트모스:버미큘라이트:펄라이트=1:1:1 처리에 비해 높았다. 그러나 실내에서는 자연토의 하중문제가 발생될 수 있으므로 추후 이를 대체할 수 있는 경량의 인공토양이 개발되어야 할 것이다. 4. 배추층의 유$\cdot$무에 의한 가는쇠고사리의 생육결과, 주 2회 관수처리에서는 배수층에 유$\cdot$무에 따른 생육적 차이가 크지 않았으나 주 7회 관수처리에서는 배수층이 있는 처리구가 생육과 실내 적응성이 높았다.

淸熱消毒飮 및 구성약물의 항균활성에 관한 실험적 연구 (The Antimicrobial Activity of Chungyulsodokeum and Its Composition Oriental Medicines)

  • 왕혜문;지선영
    • 한방안이비인후피부과학회지
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    • 제17권1호
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    • pp.143-153
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    • 2004
  • Antimicrobial activities against Streptococcus mutans, Candida albicans, Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus were assayed through the hot water extracts and the ethanol extracts from Chungyulsodokeum and its composition oriental medicines. The stains were incubated on culture medium and activated. We observed the size of inhibition zone on the strains that is incubated in strilized filter paper disc with various concentration extracts of Chungyulsodokeum and its composition 1. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Glycyrrhizae Radix, Gardeniae Fructus showed antimicrobial activities against Staphylococcus aureus. 2. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Forsythiae Fructus Ghycyrrhizae Radix, showed antimiaobial activities against Staphylococcus epidermidis. 3. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Glycyrrhizae Radix, showed antimicrobial activities against Streptococcus mutans. 4. The extract of Coptidis Rhizoma showed antimicrobial activities against Candida albicans. 5. None of the extracts showed antimicrobial activities against Escherichia coli. We observed antimicrobial activities of Chungyulsodokeum and its composition against Streptococcus mutans, Staphylococcus epidermidis, Staphylococcus aureus.

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Therapeutic Potential of Membrane Fatty Acid Modifiaction in Tumor Cells

  • Shon, Yun-Hee;Park, Kun-Young;Kim, Kwang-Soo
    • Preventive Nutrition and Food Science
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    • 제1권1호
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    • pp.143-150
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    • 1996
  • The membrane fatty acid composition of tumor cell can be modified either in cell by altering the lipid composition of the medium of during growth in animals by changing the dietaty fat composition. These modifications are associated with changes in membrane physical properties and certain cellular functions, including carrier-mediated transport and enzyme contained within the membrane. Such effects influence the transport of nutrients and chemotherapeutic agents in cancer cells .Fatty acid modification also can enhance the sensitivity of the neoplastic cell to chemotherapy. The alteration in plasma membrane composition will be affected through dietary supplementations and the potential value to cancer patients could be a better understanding of the effects of diet on responsiveness of neoplasms to chemotherapy, i.e. cancer patients' chances for a "cure" can be improved by diet changes prior to treatment.

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Optimization of Hydroxyl Radical Scavenging Activity of Exopolysaccharides from Inonotus obliquus in Submerged Fermentation Using Response Surface Methodology

  • Chen, Hui;Xu, Xiangqun;Zhu, Yang
    • Journal of Microbiology and Biotechnology
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    • 제20권4호
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    • pp.835-843
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    • 2010
  • The objectives of this study were to investigate the effect of fermentation medium on the hydroxyl radical scavenging activity of exopolysaccharides from Inonotus obliquus by response surface methodology (RSM). A two-level fractional factorial design was used to evaluate the effect of different components of the medium. Corn flour, peptone, and $KH_2PO_4$ were important factors significantly affecting hydroxyl radical scavenging activity. These selected variables were subsequently optimized using path of steepest ascent (descent), a central composite design, and response surface analysis. The optimal medium composition was (% w/v): corn flour 5.30, peptone 0.32, $KH_2PO_4$ 0.26, $MgSO_4$ 0.02, and $CaCl_2$ 0.01. Under the optimal condition, the hydroxyl radical scavenging rate (49.4%) was much higher than that using either basal fermentation medium (10.2%) and single variable optimization of fermentation medium (35.5%). The main monosaccharides components of the RSM optimized polysaccharides are rhamnose, arabinose, xylose, mannose, glucose, and galactose with molar proportion at 1.45%, 3.63%, 2.17%, 15.94%, 50.00%, and 26.81%.

작약(Paeonia lactiflora Pall.) 동아의 경정배양을 통한 기내증식 (In vivo propagation of Paeonia lactiflora Pall. Through Shoot-Tip Culture of Winter Buds)

  • 정재동;한증술;지선옥
    • 식물조직배양학회지
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    • 제22권2호
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    • pp.101-104
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    • 1995
  • 작약동아의 정아 및 액아의 기내배양에 의한 유묘의 증식에 필요한 배양조건을 구명코자 실험한 결과는 다음과 같다. 정아배양의 경우, 생장조절재의 조성에 무관하게 두지방종에서 100% 신초가 신장하였으나 생장은 의성지방종 은 NAA 0.1 mg/L 단용배지, 영천지방종은 NAA 0.01 mg/L 단용배지에서 가장 양호하였다. 액아배양의 경우, NAA 0.01 mg/L와 zeatin 5.0 mg/L 혼용배지에서 의성지방종은 100%, 영천지방종은 50%의 가장 높은 신초신장율을 보였고 신초의 생장도 양호한 경향이었다. 정아 및 액아배양으로부터 유도된 신초는 vermiculite를 지지물로 한 NAA 0.1 mg/L 첨가배지에서 30.0%의 발근율을 보였으나 뿌리의 생장은 양호하였다.

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고남산(古南山) 함(含)티탄자철광상(磁鐵鑛床)의 모암(母岩)인 알카리반려암질암의 암석화학적(岩石化學的) 연구(硏究) (Petrochemical Study on the Alkaline Gabbroic Host Rocks of Titaniferous Magnetite Deposits in Gonamsan, Yeoncheon-Gun, South Korea)

  • 장호완;염병우;박노영
    • 자원환경지질
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    • 제20권2호
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    • pp.85-95
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    • 1987
  • The host rocks of titaniferous magnetite deposits in Gonamsan are alkaline gabbros, which are typical of undersaturated alkaline rocks in terms of the lack of normative quartz. According to field occurrences and petrographic features, these alkaline gabbros are divided into 3 rock types: coarse-grained and pegmatitic rock, medium-grained rock with equigranular texture, and layered cumulate rock. All these rocks mainly contain clinopyroxene(salite), plagioclase(An 43-66), pargasite, and ilmenite. The accessory minerals are apatite, sphene, quartz, and sometimes titaniferous magnetite. Pargasite, sphene, and quartz are considered to be secondary minerals formed by the reaction among clinopyroxene, plagioclase and Fe-Ti oxide at deuteric stage. Fe-Ti oxides generally occur as ilmenite in the alkaline rocks, and titaniferous magnetite in the ore deposits. Layered cumulate rocks are characterized by a recurrence of discontinuous thin mesolayer of clinopyroxene-pargasite within leucolayer mainly composed of plagioclase. Clinopyroxene is cumulus mineral whereas plagioclase, ilmenite and apatite occur as intercumulus minerals. According to the variation diagrams of oxide and trace element contents against the differentiation index, incompatible elements, such as Na, Ba and Sr, show positive correlations whereas compatible elements, such as Mg and Cr, show negative correlations. However some compatible elements, such as Co, Ni and V show irregular variations, reflecting relative cumulate status of cumulus and intercumulus minerals. On the de la Roche multicationic diagram, these alkaline gabbros are distributed along the differentiation curve of undersaturated alkaline series from alkaline basaltic composition through basanitic composition to tephritic composition. Layered cumulate rocks, which are distributed between basanitic composition and tephritic composition, reflect their cumulate character, slightly scattering away from the curve. The medium-grained rock shows higher contents in Ba, Sr and light rare earch elements than the coarse-grained and pegmatitic rock. The former shows two times higher contents of total rare earth elements than the latter, $while(La/Lu)_{cn}$ ratios maintain fairly constant values of 5.08~5.06 in these two rocks. This means that coarse-grained and pegmatitic rock, as compared with the medium-grained rock, was formed by the earlier differentiated magma but rare-earth element distribution pattern remained almost parallel during differentiation. From the data the above mentioned, these alkaline gabbros are considered to be comagmatic and to be formed by intrusions of differentiated magmas in its reservoir.

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조기분만과 임신성 고혈압 산모의 초유내 총지질, 총콜레스테롤 및 지방산 조정 (Total Lipid, Total Cholesterol and Fatty Acid Composition in Colostrum from Mothers with Preterm Delivery and Pregnancy Induced Hypertension)

  • 안홍석
    • Journal of Nutrition and Health
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    • 제33권2호
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    • pp.186-192
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    • 2000
  • In this study, total lipid and cholesterol contents and fatty acid composition of colostrum milk obtained from 30 normal mothers, 10 mothers who was delivered of preterm infant and 8 pregnancy induced hypertensive mothers were analyzed. While total cholesterol content in preterm colostrum was significantly lower than other groups (p<0.05), total lipid content was not different among three groups, ranged 2.24-.2.2g/dl. Composition of saturated fatty acide, such as lauric acid and myristic acid which are medium chain fatty acids in preterm milk were higher than those of normal-term and hypertensive mother's milk. There was no difference n total composition of polyunsaturated fatty acids and the rationh of $\omega$6/$\omega$3 among 3 group mother's colostrum, ranged 19.45-21.45% and 6.42-7.87, respectively. but the composition of arachidonic acid and DHA in colostrum of hypertensive mothers were significantly higher than those of normal and preterm mothers. These data indicates that gestational length and complications during pregnancy may change the lipid profile and fatty acid composition of hyman milk colosstrum.

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