• 한국약용작물학회:학술대회논문집
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• 2011.04a
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• pp.422-423
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• 2011

• 한국약용작물학회:학술대회논문집
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• 2011.04a
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• pp.424-425
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• 2011

• Korean Journal of Plant Taxonomy
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• v.52 no.1
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• pp.35-44
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• 2022

Clematis pseudotubulosa (Ranunculaceae), a new species from Gyeonggi-do in the Republic of Korea, is described and illustrated. The new species is morphologically similar to C. takedana but differs in its leaves, which are always ternate with shallowly lobed leaflets (vs. 1-pinnate with deeply lobed three to five leaflets), and its oblong-shaped flower buds (vs. ovoid), larger flowers (vs. smaller flowers), and pantoporate pollen (vs. tricolpate). A detailed species description, geographical distribution, and an identification key for all species of Clematis that occur in Korea are also provided.

• Korean Journal of Plant Taxonomy
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• v.52 no.4
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• pp.214-218
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• 2022

Eleocharis R. Br. (Cyperaceae) is characterized by unbranched culms, one terminal spikelet, bisexual flowers, and bristly perianths, occurring mainly in circumboreal marshes and wetlands. There are about 250 species worldwide, and 15 taxa in Korea have been recognized. Chromosomes in Cyperaceae are known to be holocentric, missing constricted centromeres during cell division. For the first time, we report the chromosome counts of Eleocharis from Korean populations of E. attenuata f. laeviseta (Nakai) H. Hara (2n = 20); E. parvula (Roem. & Schult.) Link ex Bluff, Nees & Schauer (2n = 10); E. ussuriensis Zinserl. (2n = 16); and E. valleculosa var. setosa Ohwi (2n = 16). The populations exhibit variations in their chromosome sizes from ca.1.7 ㎛ to ca. 6 ㎛, categorized as gradient and bimodal karyotypes. The karyotypes observed are congruent with previous phylogenetic groups proposed. To clarify the traditional and phylogenetic classification of Korean Eleocharis, further taxonomic and chromosomal investigations with additional taxon sampling efforts are required.

• Korean Journal of Medicinal Crop Science
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• v.21 no.4
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• pp.247-254
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• 2013

Collected germplasms of five representative dandelion species (Taraxacum ohwianum, T. platycarpum, T. platypecidum, T. officinale, and T. coreanum) were 104 lines from different habitates in Korea and China. Their genetic diversity was analyzed by genomic fingerprinting method using amplified fragment length polymorphism (AFLP). AFLP results of 6 primer combinations were revealed 1,176 total DNA fragments and 523 polymorphic bands with a 44.4% ratio of polymorphism. On the basis of similarity coefficient analysis by unweight pair group method with arithmetic averages (UPGMA), 104 dandelion germplasm lines were ranged from 0.64 to 0.99 and clustered distinct five group depending on the species. Furthermore, a principal coordinate analysis (PCA) by the application of multi-variate analysis indicated significantly greater differences among species than geographical origins.

• The Korean Journal of Mycology
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• v.49 no.4
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• pp.497-505
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• 2021

Endophytic fungal strains were isolated from leaves, stems, and roots of Angelica gigas. The fungal strains were identified based on their morphological characteristics and molecular analysis of the internal transcribed spacer (ITS). A total 35 species of endophytic fungi were identified. The diversity between the endophytic fungal communities differed depending on the tissues of A. gigas. The isolated endophytic fungi were screened for antifungal activity against a pathogenic fungus, Phoma sp. Y11, using a dual culture method. Fourteen species of endophytic fungi showed the standout inhibition effect against the Y11 strain. The results suggest that the endophytic fungi isolated from A. gigas could be used as a biological control agent against leaf spot disease of A. gigas.

• Journal of Plant Biotechnology
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• v.48 no.1
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• pp.26-33
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• 2021

Angelica is a widely used medicinal and perennial plant. Information on the genetic diversity of Angelica populations is essential for their conservation and germ plasmic utilization. Although Angelica is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish it from other similar species from different countries. This developed single nucleotide polymorphism (SNP) markers derived from nuclear ribosomal DNA internal transcribed spacer regions genomic sequences to identify distinct Korean-specific Angelica species via amplification refractory mutation system (ARMS)-PCR curve analyses. We performed molecular authentication of different kinds of Korean-specific Angelica species such as A. gigas Nakai and A. gigas Jiri using DNA sequences in the ITS intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific Angelica species from different countr.

• Journal of Life Science
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• v.29 no.5
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• pp.524-529
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• 2019

Medicinal plants resources are becoming important assets since their usages have been expanded to the development of functional foods for human health, cosmetics and pharmaceutical industries. However, their phylogenetic origins and names are different from each country and quite often they are mixed each other resulting in the confusion for consumers. Particularly when they are very similar based on their morphological characteristics and distributed, it is extremely difficult to differentiate their origins even by specialists. Therefore, identification of each plant species is important for standardizing herbal medicine. Thistle is a medicinal and perennial plant. Obtaining information about the genetic diversity of plant populations is highly important for conservation and germplasm utilization. Although thistle is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish from other similar species from different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from chloroplast genomic sequences to identify distinct Korean-specific thistle species via high resolution melting (HRM) curve analyses. We performed molecular authentication of four different kinds of thistle species from different regions using DNA sequences in the trnL-F and matK chloroplast intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific thistle species from different country.

• Plant Breeding and Biotechnology
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• v.5 no.3
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• pp.243-251
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• 2017

Rhus chinensis is a shrub widely distributed in Asia. It has been used for traditional medicine and ecological restoration. Here, we report the complete chloroplast genome sequence of two R. chinensis genotypes collected from China and Korea. The assembled chloroplast genome of Chinese R. chinensis is 149,094 bp long, consisting of a large single copy (97,246 bp), a small single copy (18,644 bp) and a pair of inverted repeats (16,602 bp). Gene annotation revealed 77 protein coding genes, 30 tRNA genes, and 4 rRNA genes. A phylogenomic analysis of the chloroplast genomes with 11 known complete chloroplast genomes clarified the relationship of R. chinensis with the other plant species in the Sapindales order. A comparative chloroplast genome analysis identified 170 SNPs and 85 InDels at intra-species level of R. chinensis between Chinese and Korean collections. Based on the sequence diversity between Korea and Chinese R. chinensis plants, we developed three DNA markers useful for genetic diversity and authentication system. The chloroplast genome information obtained in this study will contribute to enriching genetic resources and conservation of endemic Rhus species.

• Korean Journal of Plant Taxonomy
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• v.50 no.3
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• pp.361-367
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• 2020

We report meiotic chromosome numbers of three taxa in Carex sect. Mitratae in Korea: Carex breviculmis R. Br. (n = 32_II, 33_II, 34_II), C. polyschoena H. Lév. & Vaniot (n = 37_II, 38_II), and C. sabynensis Less. ex Kunth (n = 27_II). Section Mitratae is one of the most species-rich Asian groups in Carex, comprising approximately 45-80 taxa. Twenty-seven of these occur in Korea, and they are some of the most challenging taxa to identify due to their obscure and inconspicuous diagnostic characters. Including the counts reported here, half of the native Korean sect. Mitratae chromosome numbers have been documented. Their haploid chromosome numbers range from n = 10 to n = 40, and many exhibit variations in the numbers counted within a taxon. These variations, along with the overall significant variation in sect. Mitratae, suggest that dynamic chromosome activity may be related to the high species diversity of Carex.