• 통상정보연구
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• 제17권1호
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• pp.165-191
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• 2015

본 연구는 두가지 형태로 진행되었다. 첫째는 융복합 기술과 융복합 기술이전의 선행연구 검토 및 조사분석틀 분석에 근거하여 융복합 기술이전이라는 개념화와 측정 방법의 틀을 제시하였다. 둘째, 융복합 기술이전의 분류에 근거하여 기존 기술이전 조사틀에서 최근 정책적으로 중요하게 부각되고 있는 중소기업 융복합 기술이전을 전체 중소기업 기술이전 현황과 비교하였다. 정책적으로 공공부문에서 중소기업 기술이전이 중요하며 특히 융복합 기술이전의 건수와 금액 비교를 통해 융복합 기술이전이 진행 형태를 비교할 수 있다. 비교분석을 종합하면 전반적으로 중소기업에 대한 기술이전이 증가하는 추세에도 불구하고, 융복합 기술이전은 4.4%에 불과하다. 대학 및 정출연이 보유한 융복합 기술에 대한 중소기업 기술이전은 상대적 낮은 수준이다. 산업별로 볼 때 중소기업 기술이전이 활성화된 분야는 전자부품, 기계장비, 화학, 음식료, 의료 등이며 이러한 특징은 융복합 기술이전에서도 유사한 경향을 보였다. 기술생산자별로 보면 중소기업 기술이전뿐 아니라 융복합 기술 모두에서 대학의 역할이 강조되고 있다. 기술이전 금액관점에서 1~5천만원의 건수 및 금액 비중이 높으며 5백만원 미만의 건수도 많아지는 추세이며 융복합 기술이전도 유사한 형태를 나타내고 있다. 마지막으로 지역별로 융복합 기술이전을 포함한 기술이전 전체 성과가 서울, 대전, 경기에 집중되어 있다.

• 한국항만경제학회지
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• 제25권4호
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• pp.107-130
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• 2009

부산 신항은 현재 개발 중에 있어 항만배후단지의 효유적인 이용이 부산 신항의 미래를 결정짓는 중요한 과제이다. 따라서 본 논문은 부산 신항의 항만배후단지의 효율적인 이용을 위해 우리나라의 교역량의 상당부분을 차지하고 있는 중국 및 일본과의 국제분업구조와 부산항의 대 중국 및 일본과의 수출입구조를 RCA지수와 GL지수를 이용하여 분석하고, 또한 부산 발전 10대 전략산업 등을 근거로 하여 부산 신항의 항만배후 단지에 유치할 적절한 산업을 선정하려고 한다. 한 중 일 3국의 국제분업구조 분석과 부산항의 대 중국 및 일본의 수출입실적 분석, 그리고 한 중 일 3국의 RCA 및 GL분석의 결과에 의하면, 3국간의 교역에서 한국의 비교우위 품목은 섬유 의류, 귀금속, 펄프 인쇄물, 기계 전기제품 등이며, 한국의 대 중국 및 일본교역에서는 기계 전기제품과 1차금속 비금속제품 등에서 산업내 무역이 이루어지고 있다. 여기에 더하여 광학 정밀기구 의료 악기 등이 높은 수출실적을 나타내고 있다. 또한 부산의 10대 전략산업인 의료, 섬유 의류 및 기계는 이미 포함되어 있으므로 자동차, 조선, 우주항공, 지능형 로봇, 나노소재, 연료전지 및 수소에너지 등이 추가될 수 있다. 그 결과 부산 신항의 항만배후단지에 유치할 산업은 저위기술분야인 섬유 의류와 펄프 인쇄물, 중저위기술분야인 귀금속과 1차금속 비금속제품은 물론 중고위기술분야인 기계 전기제품, 자동차, 조선, 고위기술분야인 광학 정밀기구 의료 악기, 나노소재, 연료전기, 우주항공, 지능형 로봇 등을 위주로 하고 이와 연관된 산업도 유치하는 것이 합리적이라 할 수 있다.

• The Journal of Advanced Prosthodontics
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• 제7권2호
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• pp.138-145
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• 2015

PURPOSE. The objective of this study was to conduct an in vitro comparative evaluation of polished and laser-dimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti. MATERIALS AND METHODS. Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had $5-{\mu}m$ dimples at $10-{\mu}m$ intervals, and SM30 (n=16) had $5-{\mu}m$ dimples at $25-{\mu}m$ intervals in a $2{\times}4mm^2$ area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences. RESULTS. The adhesion strength of epithelial cells was higher on Ti surfaces with $5-{\mu}m$ laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins. CONCLUSION. These findings demonstrate that laser dimpling may contribute to improving the peri-implant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

• 생명과학회지
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• 제23권2호
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• pp.324-331
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• 2013

O-GlcNAc 화(O-GlcNAcylation)는 단백질의 serine이나 threonine에 N-acetylglucosamine (GlcNAc) 분자가 결합하는 것으로, 기존의 당단백질과 달리 세포질 및 핵단백질 모두에 일어난다. 또한 수정의 속도가 빠르고 가역적으로 일어남이 인산화 수식과 유사하다. 그러나 수많은 인산화효소와 탈인산화효소가 관여하는 것과 달리 O-GlcNAc 수식은 O-GlcNAc transferase (OGT)와 O-GlcNAcase (OGA) 단 두 개의 효소에 의하여 이루어진다. 이러한 단순한 조절기전은 세포가 내외환경에 즉시 적응할 수 있도록 진화한 것으로 해석된다. 즉, O-GlcNAc 수식은 특정한 단백질 하나 하나의 활성을 켜거나 끄는 것이 아니라, 세포의 신호전달과정의 효율을 전반적으로 조절하는 '가변저항기(rheostat)' 역할을 한다. O-GlcNAc 수식은 흔히 같은 아미노산 혹은 그 주변의 아미노산이 인산화되는 것을 수반하는데, 이는 인산화와 함께 서로 조화를 이루어 세포활성을 조절하는 것으로 해석된다. 최근 O-GlcNAc이 더 나아가 O-GlcNAc-P로 인산화될 가능성이 제시되고 있는 바, 본 총설에서는 이의 가능성을 이론적으로 설명하고, 실제 실험결과를 소개한다.

• 한국환경보건학회지
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• 제17권2호
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• pp.78-94
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• 1991

The purpose of this study is to research the actual conditions of industrial accident patients and to produce worker satisfaction and a rational and effective counter measure pain. Direct interviews with 179 cases (in and out patients) were carried out during a three month period from April to July 1990, at six hospitals two general hospitals Sun Lin and Sung Mo in Po Hang, and four general hospitals in Taegu Kyung Pook University Hospital, Dong San Medical Center, Young Nam Medical Center and Catholic Hospital. The results of this study are summarized as follows: 1. Among the 179 cases, 51.6 % were male and 48.4 % were female. The two largest age groups were 30~39, 31.8 % and 20~29, 27.4 %. Among the 179 cases, 51.6% were married, the largest family number was 2 to 3, 41.1% and 4 to 5, 25.6%. Educationally, graduation from high school was the largest group, 46.4% among the patients, followed by middle school and primary school. The largest group income level was from 40~69만원, 45.2%. The largest group of patients who worked over 50 hrs. a week was 52.0%. The largest group of patients who worked less than 1 year was 44.7%, of the patients in work places of less than 100 people, 60.3% were injured and in work places of 100~299 people, 20.1% were injured. In manufacturing, the lagest group injured was 55.3%, the next group was transport, stroage, communication. The largest group of production workers injured was 40.2%. 2. The cause of injury in the largest group was facility problems, 33.5%. The next group was unsafe habits, 30.2% a lack of safety knowledge, 17.9% and insufficient supervision, 12.3%. The 30~39 year age group was head the highest number of injuries, 40.4% work places with more than 10 yeras of work, 44.4% work palces with more than 1000 people, 56.3% and mining accidents, 80.0%. Among these groups the highest cause of injury was due to facility problems. 3. The accident pattern showed machinery injuries 28.5% as the largest group, followed by falls & falling objects 17.3%, fire & electric 15.1%, struke by an object 14.5%, followed by overaction and vehicular accidents. The accident pattern showed 46.4 % among workers over the 50 year age group, workers in the 5~10 year group, 50.0 % places employing more than 1000 workers, 35.3 % : construction 73.7%, and construction workers 57.1%, among these fall & falling objects caused the greatest number of injuries. 4. The largest group of injuries was fractures 54.8%, trauma 14.5%, amputation 11.7%, open wound, and burns. The largest number of fractures occurred in people in the 30~39 year age group, 63.2 % over 10 years of work, 55.6% in work places of 300~400 people, 63.6% construction 63.2% and general workers 57.2 %. 5. The largest group of injuries was upper extremity 45.3%, lower extremity 24.0%, trunk 18.5 % and head or neck 12.2%. Of these groups, upper extremity injuries were the highest in those less 20 years old 75.0%, less than 1 years of work 59.5%, in work places of 500~999 people 60.0%, manufacturing 56.6 % and production workers 55.6%. 6. Periods of injury showed 34 people injured in September, to be the largest followed by October, 32 August, 22 people July, 19 people and the lowest December, 2 people. During the week, Friday had the largest group injured, 35 people followed by Saturday, 26 people and the lowest was Wednesday, 17 people, During the day 1400 hours had the largest group injured, 38 people followed by 800 hours, 31 people. 7. On a basis of 5 as the highest mark, the average, according to worker satisfaction showed facility safety 3.55, work environment 3.47, income 3.44, job 3.21 and treatment 2.98. 8. The correlation between general characteristics and injury showed that age was directly correlated to the duration of work(r=.2591) p<0.01, age was directly correlated to industry (r=2311) p<0.01, and the duration was directly correlated to occupation(r =.4372) p<0.001.

• Toxicological Research
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• 제8권2호
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• pp.343-357
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• 1992

Tumor necrosis factor-${\alpha}$(TNF), a polypeptide hormone secreted primarily by activated macrophages, was originally identified on the basis of its ability to cause hemorrhagic necrosis and tumor regression in vivo. Subsequently, TNF has been shown to be an important component of the host responses to infection and cancer and may mediate the wasting syndrome known as cachexia. These systemic actions of TNF are reflected in its diverse effects on target cells in vitro. TNF initiates its diverse cellular actions by binding to specific cell surface receptors. Although TNF receptors have been identified on most of animal cells, regulation of these receptors and the mechanisms which transduce TNF receptor binding into cellular responses are not well understood. Therefore, in the present study, the mechanisms how TNF receptors are being regulated and how TNF receptor binding is being transduced into cellular responses were investigated in rat liver plasma membranes (PM) and ME-180 human cervical carcinoma cell lines. $^{125}I$-TNF bound to high ($K_d=1.51{\pm}0.35nM$)affinity receptors in rat liver PM. Solubilization of PM with 1% Triton X-100 increased both high affinity (from $0.33{\pm}0.04\;to\;1.67{\pm}0.05$ pmoles/mg protein) and low affinity (from $1.92{\pm}0.16\;to\;7.57{\pm}0.50$ pmoles/mg protein) TNF binding without affecting the affinities for TNF, suggesting the presence of a large latent pool of TNF receptors. Affinity labeling of receptors whether from PM or solubilized PM resulted in cross-linking of $^{125}I$-TNF into $M_r$ 130 kDa, 90 kDa and 66kDa complexes. Thus, the properties of the latent TNF receptors were similar to those initially accessible to TNF. To determine if exposure of latent receptors is regulated by TNF, $^{125}I$-TNF binding to control and TNF-pretreated membranes were assayed. Specific binding was increased by pretreatment with TNF (P<0.05), demonstrating that hepatic PM contains latent TNF receptors whose exposure is promoted by TNF. Homologous up-regulation of TNF receptors may, in part, be responsible for sustained hepatic responsiveness during chronic exposure to TNF. As a next step, the post-receptor events induced by TNF were examined. Although the signal transduction pathways for TNF have not been delineated clearly, the actions of many other hormones are mediated by the reversible phosphorylation of specific enzymes or target proteins. The present study demonstrated that TNF induces phosphorylation of 28 kDa protein (p28). Two dimensional soidum dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) resolved the 28kDa phosphoprotein into two isoforms having pIs of 6.2 and 6.1. The pIs and relative molecular weight of p28 were consistent with those of a previously characterized mRNA cap binding protein. mRNA cap binding proteins are a class of translation initiation factors that recognize the 7-methylguanosine cap structure found on the 5' end of eukaryotic mRNAs. In vitro, these proteins are defined by their specific elution from affinity columns composed of 7-methylguanosine 5'-triphosphate($m^7$GTP)-Sepharose. Affinity purification of mRNA cap binding proteins from control and TNF treated ME-180 cells proved that TNF rapidly stimulates phosphorylation of an mRNA cap binding protein. Phosphorylation occurred in several cell types that are important in vitro models of TNF action. The mRNA cap binding protein phosphorylated in response to TNF treatment was purifice, sequenced, and identified as the proto-oncogene product eukaryotic initiation factor-4E(eIF-4E). These data show that phosphorylation of a key component of the cellular translational machinery is a common early event in the diverse cellular actions of TNF.