• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 춘계학술발표회
• /
• pp.36-36
• /
• 2018

Soybean is the important crop in Asian countries as protein source, oil production and animal feed. Improving soybean using genetic transformation is the principal tool in nowadays. Developing herbicide resistant transgenic soybean plants through Agrobacterium-mediated transformation has been worked in many previous studied. However, the transformation efficiency is still low. Many attempts try to find the optimum media condition for plant regeneration after infection. After transformation, the plant regeneration is very important condition to promote growth of transgenic plant. In this study, we optimized a regeneration condition for two Korean soybean cultivar, Dawonkong and Pungsannamulkong using cotyledon, cotyledonary nodes and hypocotyl as explant. The results showed that shoot regeneration of cotyledonary nodes on B5 medium containing 2 mg/L 6-benzylaminopurine showed the highest percentage of regeneration in Dawonkong (75.8%) while Pungsannamulkong presented high number of shoots 2.12 shoots per explant. For transformation condition, co-cultivation in 7 days showed a high number of GUS positive expression. Most of explants can survived under media including 5 mg/L of glufocinate which refers phosphinotricin for 2-week selection. Washing with 400 mg/L of cefotaxime in several times and selection in plant regeneration media with 400 mg/L of cefotaxime can prevent bacteria growth, effectively.

• 복식문화연구
• /
• 제27권5호
• /
• pp.449-460
• /
• 2019

Today, the fashion business environment of the 4.0 generation is changing based on fashion technology combined with advanced digital technologies such as AI (Artificial Intelligence), big data and IoT (Internet of Things). "Digital Transformation" means a fundamental change and innovation in a digital paradigm including corporate strategy, organization, communication, and business model, based on the utilization of digital technology. Thus, this study examines digital transformation strategies through the fashion brand Burberry. The study contents are as follows. First, it examines the theoretical concept of digital transformation and its utilization status. Second, it analyzes the characteristics of Burberry's digital transformation based on its strategies. For the research methodology, a literature review was performed on books and papers, aligning with case studies through websites, social media, and news articles. The result showed that first, Burberry has reset their main target to Millennials who actively use mobile and social media, and continues to communicate with them by utilizing digital strategy in the entire management. Second, Burberry is quickly delivering consistent brand identity to consumers by internally creating and providing social media-friendly content. Third, they have started real-time product sales and services by using IT to enhance access to brands and to lead consumers towards more active participation. In this study, Burberry's case shows that digital transformation can contribute to increased brand value and sales, keeping up with the changes in the digital paradigm. Therefore, the study suggests that digital transformation will serve as an important business strategy for fashion brands in the future.

• International Journal of Internet, Broadcasting and Communication
• /
• 제13권2호
• /
• pp.267-275
• /
• 2021

The advent of the "un-tact 2.0 era," described as a "new normal non-face-to-face social relationship," accelerates the transformation of the living paradigm as a fully digital mediated social relationship. The emergence of these new forms of digital behaviors and mediated relationships significantly influences the industry prospect and consumers' individual lives. Advertising has played a decisive role in moving the formation forces of society, creating a dynamic flow of the capitalist system across races and geographical boundaries. Its role will become more fundamental in the physical contactless environment due to the COVID-19 outbreak. Accordingly, advertising has changed and modified its shape and meaning throughout history to adapt to the dynamic external environment. In this article, we illustrated four primary stages of the evolution in the advertising industry from simple advertising to convergence of advertising. Finally, we also identified the challenges of the present advertising industry and the paradigm transformation of "un-tact 2.0" with various related examples.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 추계학술대회
• /
• pp.52-52
• /
• 2018

Development of herbicide resistant transgenic legume plants through Agrobacterium-mediated transformation has been worked in many previous studied. Plant regeneration after infection is the important step to obtain successful transgenic plants. Many attempts try to find the optimum media condition for plant regeneration after infection. However, the transformation efficiency of legume plants is still low. In this study, regeneration of some Korean legume species including two soybean cultivars (Dawon and Pungsan) and pea have been done with organogenesis which is used various kind of explants such as cotyledonary-nodes in soybean and bud-containing tissue in pea. We developed the optimum media condition for plant regeneration regulators under Agrobacterium-mediated transformation using different kind and various concentration of plant growth. As the results, B5 medium containing 2 mg/L of 6-benzylaminopurine was selected in this study for the optimum plant regeneration media. The segments were inoculated with Agrobacterium suspension harbored an IG2 vector containing bar gene which confers resistance to phosphinotricin (PPT) in 3, 5 and 7 days. The transformation efficiency was achieved in Dawon 3.03 % and pea 1.46 % with co-cultivation period of 7 days which is showed a high number of GUS positive expression period.

• 한국미생물·생명공학회지
• /
• 제13권4호
• /
• pp.345-348
• /
• 1985

Mannitol hypertonic regeneration media를 사용하는 PEG-induced protoplast transformation system을 이용해서 pUB110과 pE194의 recombinant plasmid로 B. subtilis BR151을 transformation 시킴으로써 두 plasmid에서 유래되는 각각의 Neo$^{R}$와 Em$^{R}$을 동일한 recipient cell 내에서 동시에 발현시킬 수 있었다. Neomycin과 erythromycin을 함께 함유하는 mannitol regeneration media상에서 recombinant plasmid의 transformation frequency는 6.5 $\times$ $10^{-5}$이었다. 한편 transformant cell 내에서 recombinant plasmid의 replication이 agarose gel electrophoresis로 확인되었다.

• 정보과학회 컴퓨팅의 실제 논문지
• /
• 제20권11호
• /
• pp.592-597
• /
• 2014

본 논문에서는 다양한 스마트 단말과 홈네트워크 장치가 연결된 유무선 홈네트워크 환경을 기반으로 스마트 단말을 이용하여 원격으로 홈네트워크를 편리하게 제어할 수 있도록 스마트 단말 특성에 맞게 최적화된 UI와 미디어 콘텐츠를 제공하는 적응형 변환 엔진을 제시한다. 적응형 변환 엔진은 스마트 단말 프로파일과 UI 변환 정책을 고려하여 동적으로 UI 콘텐츠를 변환하여 전송할 뿐만 아니라 단말 프로파일과 미디어 프로파일을 고려한 실시간 미디어 트랜스코딩과 전송 기능을 통하여 스마트 단말에 적합한 미디어 콘텐츠를 제공한다.

• 한국작물학회지
• /
• 제49권1호
• /
• pp.61-68
• /
• 2004

A reproducible transformation system via optimized regeneration media for Korean rice cultivars was established using Agrobacterium tumefeciens LBA4404 (pSBM-PPGN; gusA and bar). Although japonica rice genotypes were easier to produce transgenic plants compared to Tongil type cultivars, transformation efficiencies were not always correlated with regeneration efficiencies of non-transgenic callus on the control medium. Regeneration efficiencies of Donganbyeo, Ilmibyeo, and Manchubyeo were over 50% in non-transgenic control, however, transformation efficiencies were significantly low when only sucrose was added to the media as a carbon source. However, the medium, MSRK5SS-Pr (or MSRK5SM-Pr), that contains $5\textrm{mgL}^{-1}$ kinetin, $0.5\textrm{mgL}^{-1}$ NAA, 2 % sucrose (or maltose), 3% sorbitol, and $500\textrm{mgL}^{-1}$ proline, was the most efficient not only for regeneration of non-transgenic callus but also for regeneration of transgenic callus in the presence of L-phosphinotricin (PPT). Average transformation efficiencies of 16 Korean rice cultivars were significantly enhanced by using the optimized medium from 1.5% to 5.8% in independent callus lines and from 2.9% to 19.4% in tromsgenic plants obained. Approximately 98.9% (876 out of 885) transgenic plants obtained on optimized media showed basta resistance. Stable integration, inheritance and expression of gusA and bar genes were continued by GUS assay and PCR and Southern analysis of the bar gene. With Pst1 digestion of genomic DNA of transgenic plants, one to five copies of T-DNA segment were observed; however, 76% (19 out of 25 transgenic plants) has low copy number of T-DNA. The transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

• ETRI Journal
• /
• 제38권5호
• /
• pp.807-817
• /
• 2016

A novel fast algorithm is suggested for a coding unit (CU) mode decision using pseudo rate-distortion based on a separated encoding structure in High Efficiency Video Coding (HEVC). A conventional HEVC encoder requires a large computational time for a CU mode prediction because prediction and transformation procedures are applied to obtain a rate-distortion cost. Hence, for the practical application of HEVC encoding, it is necessary to significantly reduce the computational time of CU mode prediction. As described in this paper, under the proposed separated encoder structure, it is possible to decide the CU prediction mode without a full processing of the prediction and transformation to obtain a rate-distortion cost based on a suitable condition. Furthermore, to construct a suitable condition to improve the encoding speed, we employ a pseudo rate-distortion estimation based on a Hadamard transformation and a simple quantization. The experimental results show that the proposed method achieves a 38.68% reduction in the total encoding time with a similar coding performance to that of the HEVC reference model.

• 한국언론정보학보
• /
• 제33권
• /
• pp.87-114
• /
• 2006

대안적 공론장의 현실화를 지칭하는 용어는 저항언론, 대항언론, 민중언론, 지하언론, 풀뿌리언론, 대안언론, 시민미디어 등 다양한 개념이 존재하다. 시대적 상황에 따라, 논자의 강조점에 따라 다양한 개념들이 제시되고 있는 것이다. 본 논문은 한국의 언론구조가 민주적 공론장으로서 제 기능을 수행하지 못함에 따라 이를 보완하고자 했던 대안적 공론장의 변화과정을 고찰하고 있다. 정리하자면, 한국의 대안적 공론장은 70년대의 저항언론 자유언론에서, 80년대의 대항언론 민중언론, 90년대의 대안언론, 2000년대의 대안언론 시민미디어의 유형으로 변화해왔다. 이러한 변화를 추동하는 요인은 무엇보다도 시민사회의 확장과 사회운동 진영이다. 그리고 사회운동 주도세력의 성격에 따라 대안적 공론장의 내용성 역시 변화해 왔다. 80년대 민중운동이 한국의 사회운동을 이끌었을 때는 대안적 공론장도 당파성에 입각한 민중언론의 성격을 나타냈으며, 90년대 시민운동으로 그 주도권이 바뀌었을 때는 대안언론, 시민미디어의 유형으로 발현됐다. 또한, 사회운동과 대안적 공론장은 변증법적으로 변화 발전했다. 요컨대, 사회운동은 대안적 공론장을 통해 운동의 활성화와 역량을 축적하면서 사회변화를 이끌었고, 이러한 사회운동은 다시 대안적 공론장의 변화 발전으로 이어지고 있다는 것이다.

• 스마트미디어저널
• /
• 제11권10호
• /
• pp.54-64
• /
• 2022

빅데이터 기반의 디지털 트랜스포메이션은 데이터 및 데이터 관련 기술을 통해 기업의 성과 향상, 조직 변화, 사회 공헌 등의 목적 달성을 위해 수행하는 혁신적 프로세스를 의미한다. 성공적인 빅데이터 기반의 디지털 트랜스포메이션을 위해서는 관련 연구 현황, 주요 연구토픽, 주요 연구토픽 간의 관계를 이해하는 것이 필수적이다. 그러나 여러 연구들의 서로 다른 관점 및 이들 간 연계 가능성에 대해 이해하려는 노력은 아직 미진하다. 본 논문은 텍스트마이닝을 활용하여 관련 연구동향을 분석하고, 여러 연구의 다양한 관점을 통합적으로 이해하기 위한 기반 마련을 시도해보았다. Web of Science Core Collection에서 추출한 439편의 논문을 분석하여, 10개의 주요 연구토픽을 도출하였고, 이들 간의 관계를 분석하였다. 본 연구의 결과가 빅데이터 기반의 디지털 트랜스포메이션에 대한 통합적인 이해를 촉진하고, 성공을 위한 방향성 모색에 기여할 것으로 기대한다.