• Title/Summary/Keyword: Media Supplement

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Root Induction and Propagation of Sedum takesimense Nakai Using Leaf Cutting Method

  • Cheong, Eun Ju
    • Journal of Forest and Environmental Science
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    • v.34 no.1
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    • pp.50-52
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    • 2018
  • The effect of basal nutrients concentrations and exogenous auxin for root induction from leaves of Sedum takesimense were investigated for mass-propagation. Root induction rates were significantly different from the concentrations of basal salts but not influenced by supplemented IBA in the medium. The lowest concentration of MS basal salts (1/10) was most effective to induce roots from leaves followed 1/5 MS, and 1/2 and full strength MS medium. Supplement of IBA $10{\mu}M$ in the medium did not improve the root induction that resulted no differences compare to the hormone free media. Rooted leaves were transplanted in soil and survived in greenhouse.

A Study on the Methodology of Chromosome Preparation from Blood Culture (혈액세포를 이용한 염색체 분리 분석에 관한 방법적 고찰)

  • Sohn, S.H.;Chung, K.M.
    • Clinical and Experimental Reproductive Medicine
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    • v.21 no.2
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    • pp.207-214
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    • 1994
  • This study was carried out to develop the methodology of chromosome preparation from blood cultures in mammals which included human, mouse, cattle and pig. For karyotyping, 0.5-5.0ml of peripheral blood were collected and cultured. The satisfactory results were obtained from macroculture and microculture in all species. In culture, the patterns of cell growth were no difference among media except serum concentration and mitogen supplement. The presence of mitogen and fetal bovine serum in medium significantly affected the mitotic index. The optimal culture condition was 37$^{\circ}C$ for 3 days. And the concentration of colcemid and reincubation time also affected the chromosome morphology. In harvest, chromosome patterns were mainly affected on hypotonic treatment which included treated time and temperature, dropwise of fixative solution, and drying after slide preparation.

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STEREOSCOPIC EYE-TRACKING SYSTEM BASED ON A MOVING PARALLAX BARRIER

  • Chae, Ho-Byung;Lee, Gang-Sung;Lee, Seung-Hyun
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2009.01a
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    • pp.189-192
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    • 2009
  • We present a novel head tracking system for stereoscopic displays that ensures the viewer has a high degree of movement. The tracker is capable of segmenting the viewer from background objects using their relative distance. A depth camera is used to generate a key signal for head tracking application. A method of the moving parallax barrier is also introduced to supplement a disadvantage of the fixed parallax barrier that provides observation at the specific locations.

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Verification of AGB (alpha-glucosidase biosynthesis) Bacterial Bioassay of Metal Toxicity and Inhibition Effect of Fulvic Acid (EA) (박테리아(E. Coli)의 AGB (alpha-glucosidase biosynthesis)를 이용한 중금속의 독성시험과 풀빅산에 의한 독성 억제효과)

  • Kim Jae Hyoun;Kim Myung Gill;Om Ae Son
    • Environmental Analysis Health and Toxicology
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    • v.19 no.1
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    • pp.25-32
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    • 2004
  • Aim of the present study was the development of a bioassay which enables the detection of toxic effects of heavy metal ions to a bacterium, Escherichia coli. Inhibition effects of the metals on growth rates of the bacterium were studied in the absence or presence of fulvic acid. This method does not clearly differentiate among metals, but does detect overall AGB inhibition rate (toxicity) for 5 different heavy metals. The toxicity of the metals in the absence of fulvic acid in the same testing conditions was significantly increased in following order: Hg < Pb, Zn < Cd < Cu, whereas the inhibition rate (toxicity) in the presence of FA was shown to be increased In following order: Cd < Pb, Hg < Cu < Zn. The results of the present study indicate that this simple and fast biomonitoring assay with direct exposure of E coli. might be a valuable supplement to analytical methods of contaminated media.

Enhancing Effect of Egg Albumin on Ethanol Production and Its Function (Egg Albumin이 알콜생산의 증진에 미치는 영향 및 기능)

  • Kim, Heung S.;Shin, Chul S.;Wang, Shaw S.
    • KSBB Journal
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    • v.5 no.4
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    • pp.373-376
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    • 1990
  • In ethanol fermentations with Saccharomyces sake, phosphatidylcholine-egg albumin as a supplement in fermentation media was much more effective in enhancing ethanol production than linoleic acid-ergosterol. It came from the differences in alcohol-tolerance between egg albumin and ergosterol. The egg albumin was supposed to function as a nutrient rather than to form protective layers around the cells against ethanol.

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Induction of Callus and Organ in Tissue Culture of Ginseng (Panax ginseng C. A. Meyer) (고려인삼의 조직배양에 의한 캘러스 및 기관분화)

  • Choe, Gwang-Tae;Kim, Myeong-Un;Sin, Hui-Seok
    • Journal of Ginseng Research
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    • v.6 no.2
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    • pp.162-167
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    • 1982
  • Calli and leaflets of ginseng (Panax ginseng C.A. Meyer) were cultured on 1/2MS media supplement. with kinetin, 2 iP, NAA, 2,4-D and IBA to assess their capacity to regenerate embryoids and organs. Root calli produced numerous embryoids and shoots in 1/2MS medium supplemented with 2 mg/l NAA and 2mg/s 2iP, and the combination of 2 iP and NAA was more effective than the combination of kinetin and NAA in induction of embryoid and shoot from root calli. Culture of leaflet in the medium supplemented with IBA resulted in profuse root regeneration.

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Current Status Related to Providing Health Education Courses in Elementary Schools (초등학교의 보건교과목 개설 여건)

  • Moon Jung-Soon;Sohng Kyeong-Yae;Lee Jong-Eun;Park Sun-Nam
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.12 no.1
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    • pp.101-112
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    • 2005
  • Purpose: To investigate the status of health education courses in elementary schools. Method: From September to December in 2003, 607 school nurses were surveyed by questionnaires. Results: 1) Of the schools, 32.1% had health education programs as part of the school health service. A higher proportion was found in schools in suburban and rural areas. Mass media in health education was used by 75% of school nurses. Regular classes were more frequently utilized in schools in rural areas, while the classes in health-related subjects were utilized in urban areas. For 20.6% of school nurses, there was a substitute person to provide health care while the nurse was in health education classed, but 94.2% of school nurses found they had to give up health education due to pressure from other health services. 2) Of the nurses, 97.0% felt the need to provide health education courses, but 60.6% insisted that their work load needed to be modified and supplemental personnel were necessary if health education courses were to be provided. 3) More than 30% school nurses responded that educational materials and media, and concern by teachers and administrators were insufficient. 4) School nurses suggested that the content for health education courses should include healthy life and dental health care for students in lower grades, dental health care, sex education and safety for students in middle grades, and sex education and drug abuse prevention for students in higher grades. Conclusion: There is an urgent need to provide health education courses in elementary schools but conditions in the schools are not satisfactory. For successful school health services, personnel supplement, modified work loads and sufficient educational materials and media should be in place before health education courses are provided.

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Study on the Effect of Cysteine and Myo-inositol on In Vitro Maturation of Porcine Oocytes

  • Lee, B.K.;Kim, S.K.
    • Journal of Embryo Transfer
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    • v.22 no.4
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    • pp.223-227
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    • 2007
  • This study was carried out to investigate the effect of morphology of oocytes, kinds of media, cysteine and myo-inositol supplementation on IVM rate of porcine oocytes. Cumulus- enclosed oocytes were incubated in maturation NCSU-23 and TCM-199 medium with supplementation with 3, 5, 10, 20 mM myo-inositol and 0.05, 0.1, 0.5, 1.0 mM cysteine. 1. When classified by morphology, excellent, good and fair of cumulus-enclosed oocytes were incubated for 48 hrs and the IVM rate were $14.2{\pm}3.7%{\sim}58.7{\pm}4.0%$, respectively. The rate were greater in oocytes with excellent cumulus cells than those without cumulus cells. 2. The IVM rate of oocytes cultured in TCM-199 and NCSU- 23 medium supplementation or non-supplementation with 1.0 mM myo-inositol were $7.5{\pm}4.5%,\;45.0{\pm}4.8%\;and\;4.4%,\;42.5{\pm}4.2%,\;18.0{\pm}5.2%$, respectively. Supplementation with myo-inositol significantly increased the IVM rate of oocytes. 3. The IVM rate of oocytes cultured in NCSU-23 medium supplementation of 3, 5, 10, 20 mM myo-inositol for 48 hrs were $47.5{\pm}4.5%,\;57.5{\pm}4.2%,\;62.5{\pm}4.9%,\;50.0{\pm}5.2%$, respectively. The IVM rate of oocytes in NCSU-23 medium supplemented with 10 mM myo-inositol were significantly increased compared to control ($42.5{\pm}4.0%$). 4. The IVM rate of oocytes cultured for 48 hrs in NCSU-23 media supplement with 0.3, 0.5, 1.0, 2.0 mM myo-inositol were $50.0{\pm}4.5%,\;62.5{\pm}4.2%,\;52.5{\pm}4.9%,\;45.0{\pm}4.2%$, respectively. The IVM rate of oocytes in NCSU-23 medium supplemented with 10 mM cysteine were significantly increased compared to control ($42.5{\pm}4.0%$).

Potential Crisis and Opportunity in the K-pop Choreography Copyright

  • Kim, Joy
    • International Journal of Advanced Culture Technology
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    • v.9 no.3
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    • pp.253-258
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    • 2021
  • This study aims to expose the potential dangers of K-pop through the past and present of K-pop choreography copyrights and to suggest the need to secure digital choreography copyrights for the sustainability of K-pop considering national responsibility. As the content industry quickly sought countermeasures to digital transformation, such as the launch of K-pop platforms and the evolution of OMO (Online Merges with Offline) media commerce in response to changes in the industrial environment due to COVID-19, the annual export of the domestic content industry increased by 6.3% compared to the previous year. Accordingly, our copyright does not require that works be fixed in tangibles, as in Japan and Germany, on the basis of entrusting each country's legislation to determine whether to require fixation on choreographed works. On the other hand, the United States, France and the United Kingdom are demanding that it be fixed. Although choreography is at the center of K-pop and the value and influence of K-pop videos including cover dance through new media are discussed from various perspectives, copyright on choreography that needs to be resolved in business platforms for K-pop scalability The fact that there is such a big difference in problem perception is an area where we must not lose our vigilance. As the development of today's technology, the method of fixing a choreography looks very easy as an image, but at the same time can be stolen very quickly. Therefore, compared to overseas cases, it is urgent to improve the difference in perception of copyright registration for K-pop choreography and to supplement the system include the NFT.

Survey and Analysis of Citizens' Perception for Urban Ecosystem Education - Targeting Suwon City - (도시생태계 교육을 위한 시민 인식 설문조사 및 분석 - 수원시를 중심으로 -)

  • Yoo, Da-Young;Lee, Min-Gi;Kim, Nam-Choon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.23 no.6
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    • pp.75-85
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    • 2020
  • The purpose of this study was to investigate the citizens' perception of urban ecosystem and urban ecosystem education to find out how to plan and create an effective urban ecosystem and how to utilize urban ecosystem education and educational media according to age groups. To this end, an online survey of 416 Suwon citizens was conducted, and based on the responses of the respondents, cross-analysis, multiple-response analysis, and correlation analysis were conducted using the IBM SPSS Statistics Statistical Program. The study found that fewer respondents showed a high understanding of urban ecosystem concepts compared to those who recognized the importance of environmental issues. Nevertheless, most of the respondents were aware of the importance of preserving and protecting the urban ecosystem and responded positively to the inconvenience. In addition, most of the respondents were aware of the need for urban ecosystem education and were found to have different preferred information media depending on age. It has been confirmed that the establishment of facilities such as ecological learning centers and seasonal environmental schools is the top priority among all age groups. Citizens are also aware of the importance of preserving and protecting the urban ecosystem and the need for education, but it is deemed necessary to supplement it because effective urban ecosystem conservation and protection plans and systematic education are not provided that citizens can sympathize with. In addition, it is deemed that various measures should be presented in selecting responsible organizations and educational media that host the education for effective education and promotion of urban ecosystem education according to conduct urban ecosystem education.