• Title/Summary/Keyword: Media Selection

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Efficient and Reliable in vitro Regeneration System for Rubus Species as the Basis of Genetic Engineering

  • Kalai Katalin;Meszaros Annamaria;Denes Ferenc;Zatyko Jozsef;Balazs Ervin
    • Journal of Plant Biotechnology
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    • v.7 no.4
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    • pp.241-246
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    • 2005
  • Factors affecting regeneration of different Rubus varieties (blackberry, raspberry and their hybrid) were examined and a reliable regeneration system was established. Media for stock plant maintenance were tested; different explants and media were investigated to find the best circumstances for the regeneration. The effect of the commonly used antibiotics was studied to determine the most suitable one for selection of the transformants. We found that both MS and LS media supplemented by $20\;gL^{-1}$ sucrose are suitable for the stock plant maintenance. The optimal hormone content for the stock plants is $0.125\;mgL^{-1}$ 6-benzylaminopurine (BAP) with $0.01\;mgL^{-1}$ indole-3- butyric acid (IBA). The highest regeneration rate was observed on medium containing MS salts with B5 vitamins complemented with glucose, sucrose, maltose, $10\;gL^{-1}$ each, supplemented with benzylaminopurine riboside (BAR) ($2\;mgL^{-1}$) and indole-3-acetic acid (IAA) ($0.1\;mgL^{-1}$). The regenerated shoots appeared directly from the cut edges, without callus phase. Hygromycin and geneticin proved to be good selection agents for the Rubus explants, but due to their severe effect on the tissues we propose to use marker-free constructions for the transformation.

Design of SoQ-based Cooperative Communication Protocol for UWB-based Distributed MAC/WUSB Systems (UWB 기반 Distributed MAC 시스템을 위한 SoQ 기반 협력 통신 프로토콜 설계)

  • Hur, Kyeong
    • Journal of Korea Multimedia Society
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    • v.15 no.3
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    • pp.345-355
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    • 2012
  • The WiMedia Alliance has specified a Distributed Medium Access Control (D-MAC)/WUSB protocol based on UWB for high speed wireless home networks and WPANs. In this paper, we propose a novel SoQ-based cooperative communication protocol adaptive to current UWB link transmission rate and QoS measure. The proposed SoQ-based cooperative communication protocol has compatibility with current WiMedia D-MAC/Wireless USB standard and is executed at each device according to a SoQ-based Relay Node Selection (RNS) criterion.

The Effect of Maslow's Basic Needs on Conformity and Individuality in the Clothing Selection (Maslow의 기본욕구가 의복의 동조 및 개성에 미치는 영향)

  • Kang Kyung Ja;Suh Young Sook
    • Journal of the Korean Society of Clothing and Textiles
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    • v.16 no.4 s.44
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    • pp.431-441
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    • 1992
  • This research was designed to evaluate the effect of need and of demographic variables of adult women on the conformity and individuality in the selection of clothes. 1. There are factors which have effect on variables of need. Marriage status, household type, age have effect on safety need, household type and age have effect on belongingness and love need. Household type and native community have effect on self-esteem need, household type, native community, school career, frequency of contact with mass media have effect on need for self-actualizing. Native community have effect on aesthetic need, and school careers have effect on the desires to know and to understand. 2. Self-esteem need, belongingness and love need, safety need, need for self-actualizing, aesthetic need, frequency of contact with mass media and income as demographic variables are directly related with conformity of clothing. Age, marriage status, native community, household type, school career are indirectly related with conformity of clothing. 3. Self-esteem need, need for self-actualizing, aestheitic need, desires to know and to understand, belongingness and love need, frequency of contact with mass media, household type have direct effect on the individuality of clothing. And age, household type, native community, school career have indirect effect on the individuality of clothing.

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A Study on Headline Typography in Newspaper & Magazine Advertisement (신문.잡지광고에 있어서 헤드라인 서체의 활용에 따른 비교 연구)

  • 문영만
    • Archives of design research
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    • v.21
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    • pp.77-86
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    • 1997
  • Modern society has created the age of mass media image. Advertising media strongly stimulate and influence the image contact and the frequency of receivers by visual communication media in everyday life. Until today no consciousness concerning the necessity of typographic visual image and it's selection for newspaper advertising has been developed. The necessity of headline typography visual image in newspaper advertising and it's selection should be considered a problem that must be solved by a more reasonable and logical approach, like it is done in the case of advertising copy, in order to increase the efficiency of advertising for the consumer.

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Optimization of Regeneration Condition Under Agrobacterium-mediated Transformation in in vitro Cultured Korean Soybean

  • Kantayos, Vipada;Bae, Chang-Hyu
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.36-36
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    • 2018
  • Soybean is the important crop in Asian countries as protein source, oil production and animal feed. Improving soybean using genetic transformation is the principal tool in nowadays. Developing herbicide resistant transgenic soybean plants through Agrobacterium-mediated transformation has been worked in many previous studied. However, the transformation efficiency is still low. Many attempts try to find the optimum media condition for plant regeneration after infection. After transformation, the plant regeneration is very important condition to promote growth of transgenic plant. In this study, we optimized a regeneration condition for two Korean soybean cultivar, Dawonkong and Pungsannamulkong using cotyledon, cotyledonary nodes and hypocotyl as explant. The results showed that shoot regeneration of cotyledonary nodes on B5 medium containing 2 mg/L 6-benzylaminopurine showed the highest percentage of regeneration in Dawonkong (75.8%) while Pungsannamulkong presented high number of shoots 2.12 shoots per explant. For transformation condition, co-cultivation in 7 days showed a high number of GUS positive expression. Most of explants can survived under media including 5 mg/L of glufocinate which refers phosphinotricin for 2-week selection. Washing with 400 mg/L of cefotaxime in several times and selection in plant regeneration media with 400 mg/L of cefotaxime can prevent bacteria growth, effectively.

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The study on Fixed Bio-reactor Characteristics Using Porous Media (다공성 여재를 이용한 고정생물막 반응기 특성에 관한 연구)

  • 이영신;김동민;정상철;백명석
    • Journal of Environmental Health Sciences
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    • v.22 no.1
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    • pp.99-106
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    • 1996
  • The purpose of this study is to observe a specific removal efficiency of synthethetic wastewater which is managed by upflow submerged type at porous media which was sinteringed on a comparative low temperature 600$\circ$C, was annexed slag and humus soil with main material kaolinite. Observing removal efficiency quality of each media, a mixed media of kaolinite and humus soil by gravity percent 60, 40% respectively showed the most excellent removal utility, and applied predictive models for suspended culture kinetics without consideration diffusion limitation, and when analyzed kinetic which had been processed by this study the removal efficiency accompanied by carbon, nitrogen, phosphorous volumetric loading rate variation standed for a comparative large change rate 61~71%, it means the selection of the most proper load factor had a great effect on the highly removal efficiency, yield coefficient(Y) and specific microbial attach equation showed 1.53 mgVSS/mgCOD, $m_p=10039.4\times ((S_0)/(6.75+S_0))$ repectively.

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User adaptive media selection based on agent communication

  • Nunokawa, Hiroshi;Ogasawara, Naohito;Sato, Kiwamu;Suguri, Hiroki
    • Proceedings of the Korean Society for Emotion and Sensibility Conference
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    • 2000.04a
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    • pp.179-183
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    • 2000
  • By spread of Internet, we become to use several of communication such as email, ICQ, VoIP etc. But as become convenient to use, user must be aware of variety of information regarding the media and partner user. The forecast is that this problem grows larger and larger as new media are brought in the expanding communications network. In this research, we suggest an agent MIA (Media Integration Agent) that manages the information instead of user. The MIA is an agent that obtains situation of user, and dynamically exchanged addresses and tendency of using medias in form of vCard. By use of this, user can choose better communication media. Accordingly seamless communication environment that user doesn't have to be aware of various information is formed.

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A study on the resistance of saccharomyces cerevisiae to copper sulfate (유산동에 대한 Saccharomyce cerevisiae의 저항성에 관한 연구)

  • 이민재;이진기
    • YAKHAK HOEJI
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    • v.3 no.1
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    • pp.15-20
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    • 1957
  • Resume 1. The toxic effect of $CuSO_4$ on the growth of yeast began in the 0.2mM and colony formation was completely inhibited in the 3mM $CuSO_4$ media. 2. The yeast strain which was trained sucessively from lower concentration media to higher one, could grow even in 10mM $CuSO_4$ media. 3. Rlb strain produced brown pigment in copper media. 4. Resistance of Rlb strain to $CuSO_4$ did not revert in non copper media. 5. The appearance of resistant strain was regarded as the result of "Mutation and Selection". 6. The alcohol fermentation ability of Rlb strain was lower than that of parent strain. 7. Rlb strain yielded some effective substance which induced the parent strain to resist against $CuSO_4$. 8. The dehydrogenase activity of yeast was inhibited by $CuSO_4$.

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A Study on the Effective Utilization of Media for Open Education (열린교육을 위한 열린매체의 활용에 관한 연구)

  • Joo, Young-Ju
    • Journal of the Korean Institute of Educational Facilities
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    • v.5 no.3
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    • pp.107-115
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    • 1998
  • Open education is more relevant to the current educational reality which requires the liberalization, individualization and creativeness, and the effectiveness of open education will be maximized with the full utilization of instructional media. As well known, there are many different types of instructional media to promote open education such as print material, audio material, still picture, movie, computer, and multimedia. The main criteria to choose effective instructional media for open education depend upon easiness of supply and retrieval of information, and promotion of more frequent interaction among participants. In addition, utilization method, cost, curriculum contents, as well as school culture are also elements to consider in the selection of right instructional media.

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SND in the Fixed Media Tank of Membrane-BNR Process Treating Low C/N Wastewater (저농도 하수 유입 Membrane-BNR공정내 고정상담체 호기조에서의 SND)

  • Jeong, Yong-Chul;Lee, Jung-Yeol;Min, Kyung-Sok
    • Journal of Korean Society on Water Environment
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    • v.24 no.3
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    • pp.328-332
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    • 2008
  • This research was about T-N removal efficiency of oxic reactor in which fixed media submerged from Membrane BNR(An+Ax+Ox) process. This experiment was implemented by using fixed media and changing DO concentration in the oxic reactor. Nitrification efficiencies of all modes were more than 98%. When no media was in the oxic reactor, T-N removal efficiency was only 47.4%, while it were shown from 60.3% to 67.4% with packed media, which indicated improved efficiency of 27~42%. The removal efficiencies of TCOD and BOD were more than 89%, 98% respectively, which could satisfy the guideline of advanced sewage reclamation by Department of Environment. When DO concentration in the packed oxic-media tank was 0.5~1.0 mg/L, T-N removal efficiency was low, which resulted from insufficient nitrification in the oxic reactor. Therefore, DO concentration of bulk solution needs to be kept more than 1.0 mg/L to induce higher nitrification efficiency in the reactor in which media was submerged. Also, the selection of DO concentration is important to prevent media from being clogged.