• Title/Summary/Keyword: Media Flow

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Flow Cytometric Analysis of the Effect of Silkworm Hemolymph on the Baculovirus-Induced Insect Cell Apoptosis

  • Rhee, Won-Jong;Park, Tai-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.11 no.5
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    • pp.853-857
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    • 2001
  • The effect of silkworm hemolymph on the inhibition of baculovirus-induced insect cell apoptosis was quantitatively investigated using a flow cytometric analysis. Spodoptera frugiperda (Sf9) cell and Autographa californica nuclear polyhedrous virus (AcNPV) were used as a model for insect cell and baculovirus in this study, respectively. Compared with a mammalian cell cycle, the fraction of G1 cells was relatively small in the Sf9 cell cycle. Silkworm hemolymph did not affect the Sf9 cell-cycle distribution before the baculovirus infection. However, the fraction of cells which are not in the sub-G1 phase remained at a high level for 3 days after the infection in the medium without silkworm hemolymph, while it remained at a high level for 7 days after the infection in the medium supplemented with silkworm hemolymph. The fractions of apoptotic cells in the sub-G1 phase were $4.7\%$, and 4 days after infection, $22.7\%$, in the media with and without silkworm hemolymph, respectively.

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MEASUREMENT OF THREE-DIMENSIONAL TRAJECTORIES OF BUBBLES AROUND A SWIMMER USING STEREO HIGH-SPEED CAMERA

  • Nomura, Tsuyoshi;Ikeda, Sei;Imura, Masataka;Manabe, Yoshitsugu;Chihara, Kunihiro
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2009.01a
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    • pp.768-772
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    • 2009
  • This paper proposes a method for measurement three-dimensional trajectories of bubbles generated around a swimmer's arms from stereo high-speed camera videos. This method is based on two techniques: two-dimensional trajectory estimation in single-camera images and trajectory pair matching in stereo-camera images. The two-dimensional trajectory is estimated by block matching using similarity of bubble shape and probability of bubble displacement. The trajectory matching is achieved by a consistensy test using epipolar constraint in multiple frames. The experimental results in two-dimensional trajectory estimation showed the estimation accuracy of 47% solely by the general optical flow estimation, whereas 71% taking the bubble displacement into consideration. This concludes bubble displacement is an efficient aspect in this estimation. In three-dimensional trajectory estimation, bubbles were visually captured moving along the flow generated by an arm; which means an efficient material for swimmers to swim faster.

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East Asian Trade Flows of Cultural Goods: A Gravity Model Approach

  • Yu, Shasha;Park, Eui Burm
    • Asia-Pacific Journal of Business
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    • v.2 no.1
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    • pp.49-73
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    • 2011
  • With the market evaluation of economic globalization exchanges between different cultures, cultural trade has been developing at an accelerated speed, and also playing an important role in East Asian intra-regional trade. In this research the author used gravity trade model to explain the causal relationship between dependent variable trade flows and several independent variables applying with five categories cultural goods which classified in HS codes. Firstly for cultural heritage trade flow, the results indicated that economic masses of bilateral countries have no significant influences on it; GDP per capita of host country and adjacency factor with partner country have significant negative influences on it; Internet coverage ratio has improved cultural heritages exchanges in East Asian regions. Secondly for printed matter cultural goods trade flow, the distance factor has significant negative influence but common language has significant positive influence on it. Thirdly for recorded media cultural goods, only economic masses and GDP per capita of bilateral countries can improved their trade flows. Fourthly for visual arts cultural products trade flows, almost all variables we tested have significant influences on it. Fifthly for cinema photography cultural goods trade flow, the influenced factor are same with cultural heritage products except they have strong positive interaction relationship with economic masses and common language. At last, the paper figured out some important and potential sectors for cultural goods trade in East Asia and gave some suggestions to government and cultural goods product enterprises.

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Direct simulations on 2D mold-filling processes of particle-filled fluids

  • Hwang, Wook-Ryol;Kim, Worl-Yong;Kang, Shin-Hyun;Kim, See-Jo
    • Korea-Australia Rheology Journal
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    • v.21 no.3
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    • pp.193-200
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    • 2009
  • We present a direct simulation technique for two-dimensional mold-filling simulations of fluids filled with a large number of circular disk-like rigid particles. It is a direct simulation in that the hydrodynamic interaction between particles and fluid is fully considered. We employ a pseudo-concentration method for the evolution of the flow front and the DLM (distributed Lagrangian multipliers)-like fictitious domain method for the implicit treatment of the hydrodynamic interaction. Both methods allow the use of a fixed regular discretization during the entire computation. The discontinuous Galerkin method has been used to solve the concentration evolution equation and the rigid-ring description has been introduced for freely suspended particles. A buffer zone, the gate region of a finite area subject to the uniform velocity profile, has been introduced to put discrete particles into the computational domain avoiding any artificial discontinuity. From example problems of 450 particles, we investigated the particle motion and effects of particles on the flow for both Newtonian and shear-thinning fluid media. We report the prolonged particle movement toward the wall in case of a shear-thinning fluid, which has been interpreted with the shear rate distribution.

Fracture Characteristics in Geologic Media for Groundwater Flow : Review (암반의 지하수유동해석을 위한 지하매질의 열극특성 개념에 대한 고찰)

  • 배대석;송무영
    • The Journal of Engineering Geology
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    • v.5 no.2
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    • pp.201-213
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    • 1995
  • Understanding of the fracture processes in rock mass for hydrogeology necessitates such information as fracture mechanics including genesis, propagation, termination, and the relation of fracture distribution to geologic structures and fracture modelling, etc. A current status of information on fracture for groundwater flow in rock mass, however, is very paucity except on a few special fields throughout the world. The desired and reasonable approach method in the evaluation on the groundwater flow in fractured rock mass must be based on the thorough understanding of fracture processes and a simplified model representing fracture properties which would be met to natural conditions for the interpretation and prediction.

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Effects on TMR and Airflow in HDD Cavity due to Disk Damper Shapes (디스크 댐퍼 형상이 HDD 내부 유동 및 TMR에 미치는 영향)

  • Um Yo-Han;Rhim Yoon-Chul
    • 정보저장시스템학회:학술대회논문집
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    • 2005.10a
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    • pp.248-253
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    • 2005
  • The recording density of a hard disk drive is increasing so rapidly that the storage capacity of a commercial HDD in PC reaches several hundred giga bytes recently. Many technologies related to the HDD, such as servo, media, actuator dynamics, thermo and fluid dynamics, etc. must be developed together to realize high recording density. Especially, researches in the airflow inside the HDD cavity become important as the rotational speed of a disk increases. Typical problem due to the airflow is the vibration of an actuator as the airflow collides with an I-block, suspensions and sliders, that is, FIV(Flow Induced Vibration). This problem is one of the significant sources of increasing TMR so that it must be resolved. In this research, a disk damper shape has been modified to minimize the effects of airflow on the actuator. Modified disk dampers which change flow field inside HDD cavity show good effects not only on the disk (tufter but also on the vibration of an actuator. Vibrations of E- block and slider have been measured with LDV and the airflow field inside the HDD cavity has been analyzed using a commercial package to verify these effects.

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Sewage Disposal by Different Structure of Fluidized Bed Biofilm Reactor (유동층 생물반응기의 구조변화에 따른 하수처리)

  • Park, Jong-Man;Lee, Jae-Yong;Kim, Chul-Kyoung;Koh, Chang-Woong;Kim, Nam-Ki
    • Journal of Korean Society of Water and Wastewater
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    • v.18 no.2
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    • pp.181-187
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    • 2004
  • The purpose of this study is to investigate the biofilm reactors capable of doing high efficiency treatment. Vertical fluidized bed biofilm reactor(VFBBR) and spiral fluidized bed biofilm reactor(SFBBR) was used for their performence in biodegradation of artificial sewage. The factors influencing the efficiency of those reactors were compared with difference of physical condition. They had same size but different structure to gain access of its unique characteristics. When recycle solution with flow rate of 22 mL/min and artificial sewage with flow rate of 2~10 mL/min were fed into two reactors in aerobic state, the average $COD_{cr}$, removal rate for biodegradation of SFBBR was greater than VFBBR. After reactor feed sewage was constantly maintained as flow rate of 4 mL/min and the recycle solution were changed to 10~32 mL/min respectively, the average $COD_{cr}$ removal rate of artificial sewage in SFBBR was greater than VFBBR. In this experiment for addition of support media into two reactors SFBBR was 4.1% excellent than VFBBR. Above all, SFBBR excelled VFBBR in boidegradation of organic matter in sewage.

Optimizing the Manifold Design of a Fuel Cell Stack for Uniform Distribution of Reactant Gases within Fuel Cell Channels (연료전지 채널 내 균일한 유량분배를 위한 연료전지 스택의 매니폴드 디자인 최적화 연구)

  • Jo, A-Rae;Kang, Kyung-Mun;Oh, Sung-Jin;Ju, Hyun-Chul
    • The KSFM Journal of Fluid Machinery
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    • v.15 no.5
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    • pp.11-19
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    • 2012
  • The main function of fuel cell manifold is to render reactants distribution as uniform as possible into a fuel cell stack. The purpose of this study is to numerically investigate the effects of stack manifold design on reactants distribution within a fuel cell stack. Four manifold designs with different manifold entrance shapes (expansion or diffuser) and different values of the extra width between the cell outer channel and manifold side wall are considered and applied to the fuel cell stack consisting of 50 cells. Since the fuel cell stack geometry involves several millions of grid points for numerical calculations, a parallel computing methodology is employed to substantially reduce the computational time and overcome the memory requirement. The numerical simulations are carried out and calculated results clearly demonstrate that both the manifold entrance shape and extra width have a substantial influence on manifold performance, controlling the degree of flow separation and entrance length for fully developed flow in the manifold channel. Finally, we suggest the optimum design of fuel cell manifold based on the simulation results.

Numerical Study of Land/Channel Flow-field Optimization in Polymer Electrolyte Fuel Cells (PEFCs) (I) -The Effects of Land/Channel Flow-field on Current Density and HFR Distributions- (고분자전해질형연료전지의 가스 채널 최적화를 위한수치적연구(I) -가스 채널 치수가 전류밀도와 HFR 분포에 미치는영향성-)

  • Ju, Hyun-Chul
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.32 no.9
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    • pp.683-694
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    • 2008
  • The performance and durability of Polymer Electrolyte Fuel Cells (PEFCs) are strongly influenced by the uniformity of current density, temperature, species distributions inside a cell In order to obtain uniform distributions in them, the optimal design of flowfield must be a key factor. In this paper, the numerical study of land/channel flowfield optimizations is performed, using a multi-dimensional, multi-phase, non-isothermal PEFC model. Numerical simulations reveal more uniform current density and HFR(High Frequency Resistance) distributions and thus better PEFC performance with narrower land/channel width where the less severe oxygen depletion effect near the land region and more uniform contact resistance variation along the in-plane direction are achieved. The present study elucidates detailed effects of land/channel width and assist in identifying optimal flow-field design strategies for the operation of PEFCs.

Surface Composition Change of UV/Ozone Modified Polypropylene (UV/오존에 의해 개질된 폴리프로필렌의 표면 조성 변화)

  • Kim, J.I.;Ryu, S.H.
    • Elastomers and Composites
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    • v.37 no.1
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    • pp.49-56
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    • 2002
  • Polypropylene is oxidized with UV/ozone as a function of UV treatment time and ozone flow rate and its surface characteristics are investigated using contart angle measurements and XPS. The aging behavior of oxidized surface is investigated under air, water and ethylene glycol as the aging media. Adhesion strength is also investigated using a lap shear test. Polar surface energy increases with increasing UV/ozone treatment time as well as ozone flow rate. No polar surface energy change is observed under water aging, while under air aging it decreases significantly within 2-3 days and reaches the close value as that of the untreated PP. Adhesion strength increases with increasing UV/ozone treatment time as well as ozone flow rate.