• Corrosion Science and Technology
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• 제14권4호
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• pp.166-171
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• 2015

Knowledge of how metal releases from the stainless steels used in food processing applications and cooking utensils is essential within the framework of human health risk assessment. A new European standard test protocol for testing metal release in food contact materials made from metals and alloys has recently been published by the Council of Europe. The major difference from earlier test protocols is the use of citric acid as the worst-case food simulant. The objectives of this study were to assess the effect of citric acid at acidic, neutral, and alkaline solution pH on the extent of metal release for stainless steel grades AISI 304 and 316, commonly used as food contact materials. Both grades released lower amounts of metals than the specific release limits when they were tested according to test guidelines. The released amounts of metals were assessed by means of graphite furnace atomic absorption spectroscopy, and changes in the outermost surface composition were determined using X-ray photoelectron spectroscopy. The results demonstrate that both the pH and the complexation capacity of the solutions affected the extent of metal release from stainless steel and are discussed from a mechanistic perspective. The outermost surface oxide was significantly enriched in chromium upon exposure to citric acid, indicating rapid passivation by the acid. This study elucidates the effect of several possible mechanisms, including complex ion- and ligand-induced metal release, that govern the process of metal release from stainless steel under passive conditions in solutions that contain citric acid.

• 한국도로학회논문집
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• 제19권5호
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• pp.77-88
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• 2017

PURPOSES : It is necessary to clarify the rheological properties of cement paste as a basic research in the development of mechanistic concrete mix design. The rheological properties of cement paste with different binder types, mix propositions, and with/without high range water reducers have been analyzed. METHODS : In this study, ordinary Portland cement, fly-ash, blast furnace slag, silica fume, and limestone powder were used as binders. The range of water-binder ratio was 0.3-0.5, and a total of 30 different mixes have been tested. The slump flow test, V-funnel test, and Dynamic Shear Rheometer (DSR) test were performed to analyze the rheological properties of cement paste. RESULTS : As a result of the slump flow test, it was found that the composition ratio of the binder contents greatly affected the paste flow when the high range water reducers were added. The results of V-funnel test showed that when the water-binder ratio was decreased without high range water reducers, the binder composition ratio had a large effect on the passing time of the V-funnel tester, but with high range water reducers the impact of the binder composition ratio was decreased. The slump flow and V-funnel have a certain relationship with the rheological factors (yield stress and plastic viscosity), but the correlation was not significant. Finally, we proposed the M-value considering the density and specific surface area of the binder. The correlation between rheological factors and M-value were better demonstrated than experimental values, but there is still a limit to predict the rheological factor in general mix design. CONCLUSIONS :In this study, the rheological properties of cement paste were analyzed. The binder type, composition ratio of binder, and with/without high range water reducers have combined to provide the complex effects on the rheological properties of cement paste. The correlation between the proposed M-value and rheological factor was found to be better than experimental results, but needs to be improved in the future.

• Molecular & Cellular Toxicology
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• 제5권3호
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• pp.179-186
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• 2009

Hexachlorobenzene (HCB) is a bioaccumulative, persistent, and toxic pollutant. HCB is one of the 12 priority of Persistent Organic Pollutants (POPs) intended for global action by the United Nations Environment Program (UNEP) Governing Council. POPs are organic compounds that are resistant to environmental degradation through chemical, biological, and photolytic processes. Some of HCB is ubiquitous in air, water, soil, and biological matrices, as well as in major environmental compartments. HCB has effects on various organs such as thyroid, bone, skin, kidneys and blood cells and especially, revealed strong toxicity to liver. In this study, we identified genes related to hepatotoxiciy induced by HCB in human hepatocellular carcinoma (HepG2) cells using microarray and gene ontology (GO) analysis. Through microarray analysis, we identified 96 up- and 617 down-regulated genes changed by more than 1.5-fold by HCB. And after GO analysis, we determined several key pathways which known as related to hepatotoxicity such as metabolism of xenobiotics by cytochrome P450, complement and coagulation cascades, and tight junction. Thus, our present study suggests that genes expressed by HCB may provide a clue for hepatotoxic mechanism of HCB and gene expression profiling by toxicogenomic analysis also affords promising opportunities to reveal potential new mechanistic markers of toxicity.

• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.5037-5041
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• 2013

Accumulated evidence has indicated that Ephrin A1 (EFNA1) is associated with angiogenesis and tumorigenesis in various types of malignancies, including colorectal cancer (CRC). In the current study, we performed an online search using the public microarray database to investigate whether EFNA1 expression might be altered in CRC tissues. We then conducted a case-control study including 306 subjects (102 cases and 204 well-matched controls) in Xiaoshan County to assess any association between genetic polymorphisms in EFNA1 and CRC susceptibility. Searches in the Oncomine expression profiling database revealed EFNA1 to be overexpressed in CRC tissue compared with adjacent normal tissue. The rs12904 G-A variant located in the 3' untranslated region (UTR) of EFNA1 was observed to be associated with CRC susceptibility. Compared with the AA homozygous genotype, those carrying GA genotype had a decreased risk of developing CRC (odds ratio (OR)=0.469, 95% confidence interval (CI): 0.225-0.977, and P=0.043). The association was stronger among smokers and tea drinkers, however, no statistical evidence of interaction between rs12904 polymorphism and smoking or tea drinking on CRC risk was found. Our results suggest that EFNA1 is involved in colorectal tumorigenesis, and rs12904 A>G polymorphism in the 3' UTR of EFNA1 is associated with CRC susceptibility. Larger studies and further mechanistic investigations are warranted to confirm our findings.

• 공업화학
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• 제4권2호
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• pp.318-323
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• 1993

CFC-11$(CFCl_3)$의 대체품인 HCFC-123$(CF_3CHCl_2)$의 합성을 위하여 Pentachloroethane($CHCl_2CCl_3$)을 제조하고, 이것을 antimony pentahalide 촉매의 존재하에서 불화수소와 반응시켰다. $CHCl_2CCl_3$의 불소화 반응은 촉매와 반응물간의 Cl-F교환 mechanism에 의해 진행되었으며 $CHCl_2CCl_3$의 불화도는 반응온도가 높을수록 증가하는 경향을 보여 주었다. 촉매농도 또한 생성물의 불화도에 영향을 미치나 반응온도에 비해 상대적으로 낮았다. $CCl_3CFCl_2$, $CFCl_2CFCl_2$, $CF_2ClCFCl_2$와 같은 부산물의 생성원인을 규명하기 위한 실험을 실시하여 부산물 생성 mechanism을 제안하였다.

• 한국물환경학회지
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• 제33권3호
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• pp.326-333
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• 2017

The lakes and reservoirs have been reported as important sources of carbon emissions to the atmosphere in many countries. Although field experiments and theoretical investigations based on the fundamental gas exchange theory have proposed the quantitative amounts of Net Atmospheric Flux (NAF) in various climate regions, there are still large uncertainties at the global scale estimation. Mechanistic models can be used for understanding and estimating the temporal and spatial variations of the NAFs considering complicated hydrodynamic and biogeochemical processes in a reservoir, but these models require extensive and expensive datasets and model parameters. On the other hand, data driven machine learning (ML) algorithms are likely to be alternative tools to estimate the NAFs in responding to independent environmental variables. The objective of this study was to develop random forest (RF) and multi-layer artificial neural network (ANN) models for the estimation of the daily $CO_2$ NAFs in Daecheong Reservoir located in Geum River of Korea, and compare the models performance against the multiple linear regression (MLR) model that proposed in the previous study (Chung et al., 2016). As a result, the RF and ANN models showed much enhanced performance in the estimation of the high NAF values, while MLR model significantly under estimated them. Across validation with 10-fold random samplings was applied to evaluate the performance of three models, and indicated that the ANN model is best, and followed by RF and MLR models.

• Archives of Plastic Surgery
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• 제35권4호
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• pp.455-460
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• 2008

Purpose: We have classified blepharoptosis into the categories including pseudoptosis in Koreans and compared with other previous studies. Methods: Total of 250 patients(398 eyes) who underwent surgery for blepharoptosis from 1987 to 2006 were studied. By classification of Beard, patients were categorized into congenital, acquired and pseudo blepharoptosis and later they were reclassified by their etiologies. Also addition of pseudoptosis to the classification of Frueh, blepharoptosis were categorized into neurogenic type, myogenic type, aponeurotic type, mechanical type and pseudoptosis. And we divided these cases by the degree of blepharoptosis, levator function and the operation methods. Results: Out of the 250 patients, 175 patients were congenital type, 49 were acquired type and 26 were pseudoptosis. According to the mechanistic classification, 177 myogenic type, 30 aponeurotic, 7 mechanic, 8 neurogenic and 28 pseudoptosis were categorized. Regarding severity of blepharoptosis, there were 29.2% of mild, 40% of moderate, and 30.8% of severe cases. Out of the 398 cases, in terms of the operation methods, there were 39 aponeurosis plication, 184 levator resection, 5 Muller tucking, 60 Orbicularis oculi muscle flap, 66 frontalis transfer, and 21 blepharoplasty. Conclusion: The cause and degree of ptosis, and levator function are very important when considering the amount of resected muscle. There were only a few studies about blepharoptosis classification including pseudoptosis category. Therefore, through this study, we can investigate the relationship between the pseudoptosis and the others. This study could be useful for the making future management plans of blepharoptosis in Korean patients.

• 대한수의학회지
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• 제58권1호
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• pp.1-7
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• 2018

Genus Artemisia occurs as a hardy plant and has a wide range of culinary and medicinal features. In this study, we aimed to describe the melanin inhibitory activity of one Artemisia species, i.e., Artemisia capillaris Thunb. Ethanol extracts of fermented Artemisia capillaris (Art.EtOH.FT) and non-fermented Artemisia capillaris (Art.EtOH.CT) were tested for their ability to inhibit tyrosinase activity and melanin pigmentation. Both extracts showed dose-dependent inhibition against ${\alpha}$-melanocyte stimulating hormone-stimulated melanin formation and tyrosinase activity, without cytotoxicity. At $100{\mu}g/mL$, both extracts showed greater inhibition than kojic acid, the positive control. Protein expressions of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) at the transcriptional level were determined by using real-time and semi-quantitative polymerase chain reaction. To complete the mechanistic study, presences of upstream elements of MITF, the phosphorylated-extracellular signal-regulated kinase (p-ERK), and phosphorylated-mitogen-activated protein kinase kinase (p-MEK) were confirmed by using western blot analysis. Expressions of p-TYR, p-TRP-1 and p-TRP-2, downstream factors for p-ERK and p-MITF, were translationally inhibited by both extracts. Art.EtOH.FT induced more potent effects than Art.EtOH.CT, especially signal transduction effects. In summary, Artemisia capillaris extracts appear to act as potent hypopigmentation agents.

• Bulletin of the Korean Chemical Society
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• 제23권6호
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• pp.837-845
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• 2002

Based on ab initio calculations at the MP2(FULL)/6-31+G**//RHF/6-31G** level, we compare the energetic and mechanistic features of a model reaction for catalytic action of Δ?-3-ketosteroid isomerase (KSL,E.C.5.3,3.1) with those of a corresponding nonenzymatic reaction in aqueous solution. The results show that the two catalytic acid residues,Tyr14 and Asp99, can lower the free energy of activation by 8.6kcal/mol, which is in good agreement with the experimentally predicted~9 kcal/mol,contribution of electrophilic catalyses to the whole enzymatic rate enhancement. The dienolate intermediate formed by proton transfer from the substrate carbon acid to the catalytic base residue (Asp38) ins predicted to be stabilized by 12.0 kcal/mol in the enzymatic reaction, making its formation thermodynamically favorable. It has been argued that enzymes catalyzing the reactions of carbon acids should resolve the thermodynamic problem of stabilizing the enolate intermediate as well as the kinetic porblem of lowering the free energy of activation for porton abstraction. We find that KSI can successfully overcome the thermodynamic difficulty ingerent in the nonenzymatic reaction through the electrophilic catalyses of the two acid residues. Owing to the stabilization of dienolate intermediate, the reketonization step could influence the overall reaction rate more significantly in the KSI- catalyzed reaction than in the nonenzymatic reaction, further supporting the previous experimental findings. However, the electrophilic catalyses alone cannot account for the whole catalygic capability (12-13 kcal/mol), confiming the earlier experimental implications for the invement of additional catalytic components. The present computational study indicates clearly how catalytic residues of KSI resolve the fundamental problems associated with the entropic penalty for forming the rate-limiting transition state and its destabilization in the bulk solvation environment.

• 한국초등과학교육학회지:초등과학교육
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• 제30권3호
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• pp.353-366
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• 2011

An alternative vision for science inquiry that appears to be important and challenging is model-based inquiry in which students generate, evaluate and revise their explanatory model. Pre-service teachers should be given opportunities to develop and use their mechanistic explanatory models in order to participate in the practice of science and to have a sound understanding of science. With this view, this study described a case of pre-service elementary teachers' scientific modeling in magnetism. The aims of this study were to explore difficulties preservice elementary teachers encountered while they engaged in a model-based inquiry, and to examine how their understandings of the nature of scientific models changed after the model-based inquiry. The data analysis revealed that the pre-service teachers had difficulties in drawing and writing their own thinking because they had little experience of expressing their own science ideas. When asked to predict what would happen, they could not understand what it meant to make a prediction "based on their model". They did not know how to use or consider their model in making a prediction. At the end of the model-based inquiry they reached a final consensus of a best model. However, they were very anxious about whether the model was the "correct" answer. With respect to the nature of scientific models, almost all of the pre-service teachers initially viewed models only as a communication tool among scientists or students and teachers to help understand others' ideas. After the model-based inquiry, however, many of them understood that they could create, test, and revise their "own" models "by themselves". They also realized the key aspects of scientific models that a model can be changed as evidence is accumulated and a model is a knowledge production tool as well as a communication tool. The results indicated that pre-service elementary teachers' understandings of the nature of scientific models and their previous school science experiences could affect their performance on a model-based inquiry, and their experience of scientific modeling could help them enhance their understandings of the nature of scientific models.