• The Korean Journal of Pharmacology
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• v.22 no.1 s.38
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• pp.1-23
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• 1986

• The Journal of the Korean Society for Microbiology
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• v.35 no.5_6
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• pp.344-345
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• 2000

• The Korean Journal of Pain
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• v.7 no.2
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• pp.155-161
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• 1994

• The Plant Pathology Journal
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• v.15 no.2
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• pp.93-98
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• 1999

• Bulletin of the Korean Chemical Society
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• v.16 no.11
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• pp.1013-1014
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• 1995

• Proceedings of the PSK Conference
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• 2001.04a
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• pp.124.2-124.2
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• 2001

• Korean Journal of Clinical Pharmacy
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• v.14 no.1
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• pp.46-51
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• 2004

• The Korean Journal of Physiology
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• v.10 no.2
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• pp.1-10
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• 1976

The action of acetylcholine on the sodium plus potassium activated ATPase activity in the rabbit red cell membrane has been investigated and the experiments were also designed to determine the mechanism of action of acetylcholine on the ATPase activity. The following results were observed. 1. The activity of the NaK ATPase from red cell membrane is inhibited by acetylcholine. 2. The ratio of inhibition of NaK ATPase by acetylcholine is decreased by raising the potassium concentration, and is increased by raising the sodium concentration. 3. The ATPase activity is increased by small amounts of calcium but inhibited by larger amounts. The ratio of inhibition of the enzyme by acetylcholine is increased by raising the calcium concentration. 4. The inhibitory action of acetylcholine on the NaK ATPase activity was not related to the sulfhydryl group of cysteine, the hydroxyl group of threonine, or the carboxyl group of aspartic acid. 5. The inhibitory action of acetylcholine on the ATPase activity is due to amino group of the enzyme of NaK ATPase.

• Microbiology and Biotechnology Letters
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• v.48 no.2
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• pp.148-157
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• 2020

Eucalyptus oil is a rich source of bioactive compounds with a variety of biological activities and is widely used in traditional medicine. Eucalyptus citriodora is cultivated for the production of essential oils. However, the mode of antibacterial action of essential oils from E. citriodora is not well-known. This study aimed to determine the chemical components, microbial inhibitory effect, and mechanism of action of the essential oil from E. citriodora. The oil was extracted from E. citriodora leaves by hydro-distillation and the chemical components were analyzed using gas chromatography-mass spectrometry. The antibacterial activities of eucalyptus oil against gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, and Staphylococcus intermedius) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) were screened by disc diffusion method and quantitative analysis was conducted by the microdilution method. The mechanism of action of the extracted essential oil was observed using SEM and analyzed by SDS-PAGE. The major components of E. citriodora oil were citronellal (60.55 ± 0.07%), followed by dl-isopulegol (10.57 ± 0.02%) and citronellol (9.04 ± 0.03%). The antibacterial screening indicated that E. citriodora oil exhibited prominent activity against all tested strains. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against B. subtilis were 0.5% and 1.0%, respectively. The MIC and MBC concentrations against S. aureus, S. intermedius, E. coli, and P. aeruginosa were 1% and 2%, respectively. As observed by SEM, the antibacterial mechanism of E. citriodora oil involved cell wall damage; SDS-PAGE revealed decrease in protein bands compared to untreated bacteria. Thus, E. citriodora oil showed significant antimicrobial properties and caused cellular damage.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.05a
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• pp.912-917
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• 2003

In this paper a new truss modeling technique for describing the beam shear resistance mechanism is proposed based on the reinterpretation of the well-known relationship between shear and the rate of change of bending moment in a reinforced concrete beam subjected to combined shear and moment loads. The core of the model is that a new perspective on the shear resistance can be gained by viewing the internal stress filed in terms of the superposition of two base components of shear resistance; arch action and beam action. The arch action can be described as a simple tied-arch which is consisted of a curved compression chord and a tension tie of the longitudinal steel, while the beam action between the two chords can be modeled as a membrane shearing element with forming a smeared truss action. The compatibility of deformation associated to the two action is taken into account by employing an experimental factor or internal state force factor a. Then the base equation of V=dM/dx is numerically duplicated. The new model was examined by the 362 experimental results. The shear strength predicted by the internal force state factor a show better correlation with the tested values than the present shear design.