• Molecules and Cells
• /
• 제41권12호
• /
• pp.1016-1023
• /
• 2018

Regenerative orthopedics needs significant devices to transplant human stem cells into damaged tissue and encourage automatic growth into replacements suitable for the human skeleton. Soft biomaterials have similarities in mechanical, structural and architectural properties to natural extracellular matrix (ECM), but often lack essential ECM molecules and signals. Here we engineer mineralized polysaccharide beads to transform MSCs into osteogenic cells and osteoid tissue for transplantation. Bone morphogenic proteins (BMP-2) and indispensable ECM proteins both directed differentiation inside alginate beads. Laminin and collagen IV basement membrane matrix proteins fixed and organized MSCs onto the alginate matrix, and BMP-2 drove differentiation, osteoid tissue self-assembly, and small-scale mineralization. Augmentation of alginate is necessary, and we showed that a few rationally selected small proteins from the basement membrane (BM) compartment of the ECM were sufficient to up-regulate cell expression of Runx-2 and osteocalcin for osteoid formation, resulting in Alizarin red-positive mineral nodules. More significantly, nested BMP-2 and BM beads added to a non-union skull defect, self-generated osteoid expressing osteopontin (OPN) and osteocalcin (OCN) in a chain along the defect, at only four weeks, establishing a framework for complete regeneration expected in 6 and 12 weeks. Alginate beads are beneficial surgical devices for transplanting therapeutic cells in programmed (by the ECM components and alginate-chitosan properties) reaction environments ideal for promoting bone tissue.

• 한국지반공학회논문집
• /
• 제22권11호
• /
• pp.89-99
• /
• 2006

프리캐스트 콘크리트 옹벽은 현장타설 콘크리트 옹벽에 비해 공기 단축효과, 품질의 우수성, 교통 및 인접구조물과의 간섭 최소화 등 많은 장점을 가지고 있다. 하지만 지반조건이 비교적 양호한 직접기초형태의 경우에는 별도의 보강조치를 고려하지 않으면 자중만으로 저항하게 됨에 따라 옹벽의 단면이 증가하게 되고 그에 따른 운반상의 어려움 및 공사비 증가 등의 단점이 있다. 본 연구에서는 이러한 문제점을 보완하기 위한 방법으로 마이크로파일공법의 적용성을 평가해 보았다. 마이크로파일공법은 소형장비로 시공이 가능하므로 작업공간의 제약이 없고, 저비용 및 고효율의 장점을 가지고 있으나 보강효과를 높이기 위해 설치한 마이크로파일의 역학적 거동특성이 명확히 규명되지 않은 상태이며, 마이크로파일의 길이, 직경, 설치각도 및 설치위치 등에 대한 설치기준이 없고 경험에 의존하고 있는 실정이다. 따라서 본 연구에서는 프리캐스트 콘크리트 옹벽에 보강된 마이크로파일의 최적보강형태 및 보강효과를 규명 제시하기 위하여 활동 및 전도거동에 대한 실내모형실험을 각각 시행하였다. 또한 실내모형실험 결과를 토대로 제시된 마이크로파일의 최적보강형태를 검증하기 위한 목적으로 유한요소해석 및 한계평형해석 등의 수치해석을 수행하여 마이크로파일의 최적 보강형태를 제시하였다. 실내모형실험과 수치해석으로 프리캐스트 콘크리트 옹벽에 보강되는 마이크로파일의 최적보강형태를 평가한 결과, 마이크로파일은 옹벽높이의 0.4배 저판길이의 0.04배 일 때가, 최적의 보강효율을 보이는 것으로 평가되었다.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2015년도 학술발표회
• /
• pp.237-237
• /
• 2015

The district of Marlborough has had more than its share of river management projects over the past 150 years, each one uniquely affecting the geomorphology and flood hazard of the Wairau Plains. A major early project was to block the Opawa distributary channel at Conders Bend. The Opawa distributary channel took a third and more of Wairau River floodwaters and was a major increasing threat to Blenheim. The blocking of the Opawa required the Wairau and Lower Wairau rivers to carry greater flood flows more often. Consequently the Lower Wairau River was breaking out of its stopbanks approximately every seven years. The idea of diverting flood waters at Tuamarina by providing a direct diversion to the sea through the beach ridges was conceptualised back around the 1920s however, limits on resources and machinery meant the mission of excavating this diversion didn't become feasible until the 1960s. In 1964 a 10 m wide pilot channel was cut from the sea to Tuamarina with an initial capacity of $700m^3/s$. It was expected that floods would eventually scour this 'Wairau Diversion' to its design channel width of 150 m. This did take many more years than initially thought but after approximately 50 years with a little mechanical assistance the Wairau Diversion reached an adequate capacity. Using the power of the river to erode the channel out to its design width and depth was a brilliant idea that saved many thousands of dollars in construction costs and it is somewhat ironic that it is that very same concept that is now being used to deal with the aggradation problem that the Wairau Diversion has caused. The introduction of the Wairau Diversion did provide some flood relief to the lower reaches of the river but unfortunately as the Diversion channel was eroding and enlarging the Lower Wairau River was aggrading and reducing in capacity due to its inability to pass its sediment load with reduced flood flows. It is estimated that approximately $2,000,000m^3$ of sediment was deposited on the bed of the Lower Wairau River in the time between the Diversion's introduction in 1964 and 2010, raising the Lower Wairau's bed upwards of 1.5m in some locations. A numerical morphological model (MIKE-11 ST) was used to assess a number of options which led to the decision and resource consent to construct an erodible (fuse plug) bank at the head of the Wairau Diversion to divert more frequent scouring-flows ($+400m^3/s$)down the Lower Wairau River. Full control gates were ruled out on the grounds of expense. The initial construction of the erodible bank followed in late 2009 with the bank's level at the fuse location set to overtop and begin washing out at a combined Wairau flow of $1,400m^3/s$ which avoids berm flooding in the Lower Wairau. In the three years since the erodible bank was first constructed the Wairau River has sustained 14 events with recorded flows at Tuamarina above $1,000m^3/s$ and three of events in excess of $2,500m^3/s$. These freshes and floods have resulted in washout and rebuild of the erodible bank eight times with a combined rebuild expenditure of $80,000. Marlborough District Council's Rivers & Drainage Department maintains a regular monitoring program for the bed of the Lower Wairau River, which consists of recurrently surveying a series of standard cross sections and estimating the mean bed level (MBL) at each section as well as an overall MBL change over time. A survey was carried out just prior to the installation of the erodible bank and another survey was carried out earlier this year. The results from this latest survey show for the first time since construction of the Wairau Diversion the Lower Wairau River is enlarging. It is estimated that the entire bed of the Lower Wairau has eroded down by an overall average of 60 mm since the introduction of the erodible bank which equates to a total volume of $260,000m^3$. At a cost of $$0.30/m^3$ this represents excellent value compared to mechanical dredging which would likely be in excess of $$10/m^3$. This confirms that the idea of using the river to enlarge the channel is again working for the Wairau River system and that in time nature's "excavator" will provide a channel capacity that will continue to meet design requirements.