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Variation of Anthocyanin and Protein Contents in Glycine max L. (Merr) (Soybean) Germplasms from Korea

  • Choi, Yu Mi;Lee, Sukyeung;Hyun, Do-Yoon;Ko, Ho-Cheol;Rho, Nayoung;Hur, On-Sook;Yoon, Hyemyeong;Lee, Myung-Chul;Oh, Sejong;Shin, Myoung-Jae;DESTA, Kebede Taye
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.13-13
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    • 2019
  • Soybean (Glycine max L. (Merr) is commonly consumed and found in major foods including soymilk, soy sauce, tofu, and soy sprout in Korea and east Asia. In addition, it is common to cook the whole seeds with rice. Soybean is known to have ranges of health benefits including antiaging, anticancer, neuroprotective and antidiabetic taken either as supplement or dietary food. Anthocyanins and flavonoids in G. max are found to be the main contributors to such wide arrays of health benefits. Due to increasing economic values of soybean, development of specialty soybean cultivars is becoming an area of interest worldwide. In this study, 746 black soybean accessions from National Agrobiodiversity Center were characterized as part of an attempt to identify important germplasms of G. max. Seed coats of each accession were analyzed for their total anthocyanin, cyanidin 3-O-Glucoside (C-3-O-G), delphinidin 3-O-glucoside (D-3-O-G), petunidin-3-O-glucoside (Pt-3-O-G), and their whole seeds for crude protein contents. HPLC was used to determine and quantify the anthocyanin compositions while crude protein was determined using Kjeldahl method by Kjeltec auto-analyzer (Kjeltec 8400, Foss, Sweden). Accessions were grouped according to their anthocyanins and protein contents; the mean content of which were correlated to agronomic traits including maturity date, one hundred seed weight, cotyledon color and seed lust color. The results indicated that the total anthocyanin content (TAC) ranged from 273.77 to 6250.52 mg/100 g, with mean value of 1853.03 mg/100 g while the crude protein content (CPC) being between 33.43 and 47.51%, with mean value of 40.81%. The highest number of accessions (45.97%) showed TAC between 1000~1900 mg/100 g while 30.96% of accessions showed CPC between 41~43%. Among the 746 accessions considered, 11 (IT142935, 175818, 175855, 177191, 177209, 177211, 177214, 177216, 177218, 177220, 177274) of them showed TAC above 4000 mg/100 g. C-3-O-G was found to be the major contributor to TAC showing strong correlation. Accessions with green cotyledon color showed high mean TAC compared to those having yellow cotyledon color, and accessions with dull seed lust color showed high mean TAC than those having shiny seed lust color. One hundred seeds weight and maturity date showed positive correlation with all anthocyanin contents, except for Pt-3-O-G in the latter case. The overall result of the present study could be used as background for developing new black soybean cultivars and breeds with high anthocyanin and protein contents. The result depicted that many of the accessions could be used as potential parental lines.

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A Clinical Study of Serum Thyroglobulin Levels in Patients with Thyroid Tumor (갑상선종양환자(甲狀腺腫瘍患者)에서의 혈청(血淸) Thyroglobulin치(値)에 관(關)한 임상적(臨床的) 고찰(考察))

  • Lee, Choong-Kyu;Yu, Byung-Hee;Lee, Woo-Hyung;Yoo, Hyung-Joon;Lee, Chong-Suk
    • The Korean Journal of Nuclear Medicine
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    • v.17 no.1
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    • pp.25-31
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    • 1983
  • Serum thyroglobulin(Tg) was measured by radioimmunoassay in 81 patients with thyroid tumor who were treated in the department of Internal medicine of National Medical Center from January, 1981 to June, 1982. The results were as follows: 1) The mean serum thyroglobulin level in thyroid tumor was significantly higher than normal subjects (p<0.001). 2) The mean serum thyroglobulin level in benign tumor was lower than malignant tumor (p<0.05). 3) The thyroid carcinoma with metastasis had significant high level of serum thyroglobulin than without metastasis (p<0.001). 4) The mean postoperative serum thyroglobulin level was significantly lower than preoperative level (p<0.001). Data from our study show that serum thyroglobulin determination is useful for assessing the presence of malignant thyroid tumor, metastasis and the extent of residual or recurrent thyroid cancer after surgery.

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Factors Affecting Tibial Tuberosity-Trochlear Groove Distance in Recurrent Patellar Dislocation

  • Prakash, Jatin;Seon, Jong-Keun;Ahn, Hyeon-Woon;Cho, Kyu-Jin;Im, Chae-Jin;Song, Eun Kyoo
    • Clinics in Orthopedic Surgery
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    • v.10 no.4
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    • pp.420-426
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    • 2018
  • Background: The tibial tuberosity-trochlear groove (TT-TG) distance is used to determine the necessity of tibial tubercle osteotomy. We conducted this study to determine the extent to which each of the tibial tuberosity lateralization, trochlear groove medialization, and knee rotation angle affects the TT-TG distance in both normal and patella dislocated patients and thereby scrutinize the rationale for tuberosity transfer based on the TT-TG distance. Methods: Retrospective analysis of rotational profile computed tomography was done for patella dislocated and control group patients. Femoral anteversion, tibial torsion, knee rotation angle, tuberosity lateralization, and trochlear groove medialization were assessed in all patients. Relationship of these parameters with the TT-TG distance was investigated to evaluate their effects on the TT-TG distance. Results: We observed that the patellar dislocation group, compared to the control group, had increased TT-TG distance (mean, 19.05 mm vs. 9.02 mm) and greater tuberosity lateralization (mean, 64.1% vs. 60.7%) and tibial external rotation in relation to the femur (mean, $7.9^{\circ}$ vs. $-0.81^{\circ}$). Conclusions: Tuberosity lateralization and knee rotation were factors affecting patellar dislocation. These factors should be considered in addition to the TT-TG distance to determine the need for tibial tubercle osteotomy in patients with patellar dislocation.

Effects of Food Quality and Temperature on life History Traits of Moina macrocopa Reared in Laboratory

  • Yoon, Seong Myeong;Lee, Jung Sup;Jung, Suk Hun;Baek, Kyoung Hwan;Chang, Cheon Young;Lee, Sung Kyu;Kim, Won
    • Animal cells and systems
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    • v.4 no.4
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    • pp.329-336
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    • 2000
  • Life table experiments were performed in order to examine the effects of food quality on Moina macrocopa fed with four kinds of algal foods, Botryococcus sp., Scenedesmus subspicatus, Selenastrum capricornutum, and Chlorella sp., at $20^{\circ}C$ . The temperature effects on M. macrocopa were also assessed, feeding Chlorella at $17^{\circ}C$,$20^{\circ}C$,$25^{\circ}C$, and a combination of $28^{\circ}C$ (light) and $25^{\circ}C$ (dark). The cartilaginous Botryococcs cells were Inappropriate food for Moina. Among the foods tested, Chlorella was the food of the best quality in all accounts of life history traits. Moina grown on Chlorella showed higher net reproductive rate ($R_0$), longer mean and maximum longevities, earlier mean age at maturity, longer mean carapace length at maturity, larger mean clutch size, and shorter mean time interval between clutch productions than those grown on Selenastrum and Scenedesmus. An optimal temperature for Moina was $20^{\circ}C$ . When Moina were grown on Chlorella at $20^{\circ}C$, they showed the highest r, the highest $R_0$, the shortest T, the longest mean longevity, the earliest mean age at maturity, the longest mean carapace length at maturity, and the largest mean clutch size. The results of life table experiments showed that the individual and population growth patterns were much more affected by low temperature $17^{\circ}C$, than by high temperature ($\geq 20^{\circ}C$). In the optimal condition, the r value was very high, 5.1 in $d^{-1}$. In conclusion, the food quality and the temperature are the most important factors to govern the size and continuity of Moina population, by which the individual growth rates and reproductivity of members in the population can be controlled to survive in their environment such as small and temporary water bodies in nature.

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An Experimental Study of Smoke Movement of the Various Fire Location in Room (실내공간에서 화재 발생위치에 따른 연기거동에 대한 실험연구)

  • Yu, Hong-Seon;Jeong, Jin-Yong;Lee, Jae-Ha;Hong, Gi-Bae
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.26 no.5
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    • pp.703-709
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    • 2002
  • In order to investigate the smoke movement in three dimensional room fires, the center fire, wall fire and corner fire plume in different sized fires were studied experimentally by rectangular pool fire using methanol as a fuel. As the fire size became larger for the center fires placed at the center of the floor, the air flow rate entrained through the opening, average hot layer temperature, flame angle deflected backwards and mean flame height was observed to increase. On the other hand, as the fire size became smaller, the neutral plane height in the door and time reached steady-state was observed to decrease. The average hot layer temperature, mean flame height and doorway neutral plane height obtained from comer fire were higher than those produced by wall fires and center fires. The simple model for describing the effect of walls on the mean flame height was presented. It was shown that the model provides a good description of the present measurements, when used with the assumption by Hansell(1993), that the increase of the average flame height is equal to the ratio of the open to the total perimeters of the trays. Also the two models for predicting the effects of walls on the mean flame height were presented. These models overestimated the measured values of the mean flame height above fuel trays close to a wall and in a corner by approximately 19-26%, respectively.

Clavicle Nonunion: Matchstick Bone-grafting and Osteosynthesis

  • Park, Yong Bok;Yoo, Jae Chul;Park, Geun Min;Kum, Dong Ho;Tauheed, Mohammed;Jeong, Jeung Yeol
    • Clinics in Shoulder and Elbow
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    • v.19 no.1
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    • pp.33-38
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    • 2016
  • Background: This study was conducted to evaluate the surgical outcomes of plate fixation using autologous iliac bone graft shaped in the form of a matchstick in clavicle nonunion resulting from prior surgical or non-surgical management. Methods: From May 2005 to February 2013, 17 patients underwent surgery for clavicle nonunion. The mean age at the time of surgery was 48.8 years. The iliac bone was harvested and shaped into slivers approximately 3 cm long, which resembled matchsticks. After opening of the medullary canal, the plate and screws were applied and cancellous bone was placed at the nonunion site. Matchstick bone grafts which were longitudinally placed and encircled on the nonunion site were tied with periosteum using 3-4 stitches. Union was determined via postoperative plane X-ray. Clinical status was evaluated using the visual analogue scale score for pain, and Constant and American Shoulder Elbow Surgeon score. Results: All patients had a stable radiological union at the follow-up. The mean duration from index operation to nonunion operation was 13.2 months (7-32 months). The mean follow-up period was 20.1 months (8-56 months), and the mean duration until union was 11.2 weeks (8-16 weeks). All clinical scores were improved at the final follow-up (p<0.001). The mean segmental defect was $3.3{\pm}2.6mm$ (1-18 mm); and the difference in clavicular length between operative and non-operative site was $5.9{\pm}6.9mm$. Conclusions: Matchstick shaped autologous iliac bone grafting technique in clavicle nonunion is acceptable with a high union rate.

Analysis of Plantar Pressure Differences between Flat Insole Trekking Shoes and Nestfit Trekking Shoes (네스핏 트레킹화와 평면 인솔 트레킹화의 족저압력 분석)

  • Choi, Jae-Won;Lee, Joong-Sook
    • Korean Journal of Applied Biomechanics
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    • v.25 no.4
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    • pp.475-482
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    • 2015
  • Objective : The purpose of this study was to investigate mean plantar foot pressure, maximum plantar pressure and ground reaction force, and center migration path of pressure according to the type of trekking shoes for the development of shoes. Method : Subjects of the study averaged $22.10{\pm}2.05years$ of age. Their average height was $169.27{\pm}7.62cm$ and their average weight was $64.34{\pm}10.22kg$. The method of this study was administered measuring 50 steps, at once, 3 times at a speed of 4 km/h and using the data of 30 steps. Pedar-X system measured the mean foot pressure, maximum foot pressure, mean maximum force, and center migration path of pressure by subjects' position while walking. Statistical analysis was performed by SPSS 23.0 using a paired t-test. Results : Results of the study showed Nestfit trekking shoes lower foot pressure of both feet in mean foot pressure and maximum foot pressure. Nestfit trekking shoes showed high ground reaction force (p<.001) in the midfoot, and low mean ground reaction force in the rearfoot. The center migration path of pressure showed the Nestfit trekking shoes were more stable than flat insole trekking shoes. Conclusion : It can be concluded that wearing Nestfit trekking shoes spreads pressure efficiently and induces walking stability because Nestfit trekking shoes spread the pressure of the forefoot and rearfoot to the midfoot and the center migration path of pressure shows regularly.

A Study on the Job Stress and Job Satisfaction of Call Center Employees (콜센터 종사자의 직무스트레스와 직무만족도에 관한 연구)

  • Shin, Hye-Young;Kim, Oh-Woo
    • Journal of Distribution Science
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    • v.14 no.5
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    • pp.91-96
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    • 2016
  • Purpose - Although various studies have been conducted on the stress of service employees, there are still lack of studies regarding job stress and job satisfaction of call center workers. Especially there are quite few studies on the job stress according to employment type. This study focused on job stress and job satisfaction for call center employees and the correlation between the two factors and aimed to provide basic materials for seeking for the plans to reduce job stress and improve job satisfaction. Research design, data, and methodology - Frequency, percentage, and mean value were calculated through descriptive statistics in order to find out demographic characteristics, level of job stress, and job satisfaction. Differences in job stress according to employment type were calculated by using one-way ANOVA. Correlation between job stress and job satisfaction were identified through empirical analysis with Pearson's correlation coefficient. 150 materials were used for final analysis. The collected materials were analyzed to get statistics by using SPSS 20. Results - First, as for the job stress of call center workers, overall mean value was 2.54 in 4-point scales. Among the six sub-factors, job demands had the highest score, which was 2.67. Second, as for the job stress according to employment type, others showed higher score than mean value followed by contract job and full-time job in that order, in terms of job insecurity and organizational system. In terms of inappropriate remuneration, contract job showed higher score than mean value followed by others and full-time job. Third, as for the satisfaction with job, the mean value was 2.37 in 4-point scale and "very much satisfied" was only 3.3%. Lastly, in terms of job stress and job satisfaction, all sub-factors except for job demands showed significant correlation. The more job stress increased, the more job satisfaction decreased. Conclusions - First, as a result of analyzing job stress according to the employment type of call center workers, job stress increased more when the employment type was not full-time. Therefore, it was assumed that self-rescue efforts should be followed for effective employment management of call center business where contract employment takes most part as well as efforts to transfer them to full-time job. Second, decrease in job satisfaction of call center workers may affect the performance of an organization as well as service quality of the company providing the service. Therefore, various supports are required to decrease job stress and increase job satisfaction for call center workers through the expansion of rest area or break time. Third, I could recognize that there were lack of academic research on call center business in the whole service industry. Therefore, further research should be conducted more actively in the future. In particular, this study has special significance in the aspect that there were few studies on the job stress of call center workers according to employment type.

Efficacy of Unilateral Pallidotomy for Parkinson's Diesease (파킨슨씨병 환자에서 편측 담창구파괴술의 효과)

  • Cho, Woo Jin;Lee, Kyung Jin;Ji, Cheul;Park, Sung Chan;Park, Hea Kwan;Jo, Jung Ki;Cho, Kyung Keun;Rha, Hyung Kyun;Kang, Joon Ki;Choi, Chang Rak
    • Journal of Korean Neurosurgical Society
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    • v.30 no.8
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    • pp.976-980
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    • 2001
  • Objectives : For Parkinsonian patients who had not reacted favorably on drug therapy are good candidate for ventroposterolateral pallidotomy, although not curative. We studied these patients after unilateral pallidotomy, to confirm the effectiveness and safety of this procedure. Methods : We evaluated the 17 patients with idiopathic Parkinson's diesease who had undergone unilateral posteroventral pallidotomy. All patients responded to levodopa initially. Mean age was 55 years(38-75years), and mean duration of disease was 9.8 years(3-20years). Pre-and postoperative evaluation at 3 month intervals included Unified Parkinson's Disease Rating scale(UPDRS) scoring, Hoehn and Yahr(H & Y) staging, and neuropsychological examinations. Results : Pallidotomy significantly improved parkinsonian symptom(tremor, rigidity, bradykinesia, dyskinesia, sensory symptom). Nine of 10 patients who showed dyskinesia preoperatively significant improvement. The mean dose of levodopa in 9 patients was lowered. The mean H & Y score and UPDRS score were improved in on and/or off time in 15 patients. Among patients who were not improved, one patient worsened, and the others showed no change. The mean overall UPDRS off score changed from 76 preoperatively to 44(33%) at 6 months and from 70 to 52(25%) at 1 year. Transient surgical morbidity was showen in four patients and included dysarthria, hypotonia and confusion. Conclusion : We conclude that pallidotomy is safe and effective in patients who have levodopa-reponsive parkinsonism with severe symptom fluctuation. Unilateral pallidotomy also considered helpful to ipsilateral symptom. Unilateral pallidotomy can improve all of parkinsonian's symptom and allow to reduce the levodopa medication. Most of patients show satisfactory results.

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The Diagnostic Significances of Serum Carcinoembryonic Antigen in Gastrointestinal Tract Cancers (위장종양(胃腸腫瘍)에 있어서 혈청(血淸) Carcinoembryonic Antigen의 진단적(診斷的) 의의(意義)에 관(關)한 연구(硏究))

  • Kim, Jong-Tae;Won, Kyung-Hee;Kim, Yul-Ja;Lee, Chong-Suk;Lee, Hak-Choong
    • The Korean Journal of Nuclear Medicine
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    • v.17 no.1
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    • pp.1-10
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    • 1983
  • Carcinoembryonic antigen (CEA) levels were measured in the serum of 35 normal control subjects and 179 cases of various benign and malignant gastrointestinal diseases. Malignant gastrointestinal tumors include 69 cases of stomach cancer, 24 cases of hepatoma and 33 cases of colorectal cancer. Benign gastrointestinal diseases include 29 cases of peptic ulcer and 24 cases of liver cirrhosis. The results were as followings: 1) Mean serum CEA level in normal control subjects was $6.9{\pm}3.3ng/ml$ and there was; no difference in mean serum CEA level between age and sex difference. 2) In malignant gastrointestinal tumors, mean serum CEA level in colorectal cancer, hepatoma and stomach cancer, were $54.3{\pm}88.9ng/ml,\;62.1{\pm}99.7ng/ml$ respectively. Serum CEA level showed positive rate of 67% in colorectal cancer, 63% in hepatoma and 62% in stomach cancer. There was no difference in mean levels and positivity of serum CEA between these 3 malignant tumor groups. 3) Positivity of serum CEA was 61% in malignant gastrointestinal tumor group in spite of 37% in benign gastrointestinal disease group. In both mean level and positivity of serum CEA, stomach cancer was much higher than peptic ulcer. But there was no difference in mean level and positivity of serum CEA level between hepatoma and liver cirrhosis. 4) In hepatoma serum CEA level showed positive rate of 62.5% and alpha-feto protein showed a rate of 58.3%. 5) Mean serum CEA levels in patients with cancer in rectal, cecal, sigmoid colon, ascending: colon and descending colon were $73.7{\pm}106.7ng/ml,\;69{\pm}84.8ng/ml$, $15.7{\pm}9.1ng/ml,\;7.5{\pm}10.6ng/ml$ and 4.0ng/ml respectively. Positive rate of serum CEA showed 86% in sigmoid. colon cancer, 68% in rectal cancer and 66% in cecal cancer. 6) In considering of histological background, there was no correlation between the degree of differentiation of tumor cell and the serum CEA level in colorectal cancer. According to Duke's classification, the mean serum levels of CEA were $8.8{\pm}11.4ng/ml$ in group A, $15.3{\pm}16.0ng/ml$ in group B and $68.5{\pm}101.5ng/ml$ in group C respectively. Positivity-of serum CEA in group A, Band C were 40%, 50% & 69% respectively. So there was significant correlation between the degree of elevation of serum CEA and tumor extension.

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