• 제목/요약/키워드: MeCP2

검색결과 266건 처리시간 0.021초

Comparative energy content and amino acid digestibility of barley obtained from diverse sources fed to growing pigs

  • Wang, Hong Liang;Shi, Meng;Xu, Xiao;Ma, Xiao Kang;Liu, Ling;Piao, Xiang Shu
    • Asian-Australasian Journal of Animal Sciences
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    • 제30권7호
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    • pp.999-1005
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    • 2017
  • Objective: Two experiments were conducted to determine the content of digestible energy (DE) and metabolizable energy (ME) as well as the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in barley grains obtained from Australia, France or Canada. Methods: In Exp. 1, 18 growing barrows ($Duroc{\times}Landrace{\times}Yorkshire$; $31.5{\pm}3.2kg$) were individually placed in stainless-steel metabolism crates ($1.4{\times}0.7{\times}0.6m$) and randomly allotted to 1 of 3 test diets. In Exp. 2, eight crossbred pigs ($30.9{\pm}1.8kg$) were allotted to a replicate $3{\times}4$ Youden Square designed experiment with three periods and four diets. Two pigs received each diet during each test period. The diets included one nitrogen-free diet and three test diets. Results: The relative amounts of gross energy (GE), CP, and all AA in the Canadian barley were higher than those in Australian and French barley while higher concentrations of neutral detergent fiber, acid detergent fiber, total dietary fiber, insoluble dietary fiber and ${\beta}-glucan$ as well as lower concentrations of GE and ether extract were observed in the French barley compared with the other two barley sources. The DE and ME as well as the SID of histidine, isoleucine, leucine and phenylalanine in Canadian barley were higher (p<0.05) than those in French barley but did not differ from Australian barley. Conclusion: Differences in the chemical composition, energy content and the SID and AID of AA were observed among barley sources obtained from three countries. The feeding value of barley from Canada and Australia was superior to barley obtained from France which is important information in developing feeding systems for growing pigs where imported grains are used.

Energy Expenditure in Crossbred Cattle Fed Paddy Straw of Different Form

  • Bhatta, Raghavendra;Kumar, Vijay;Sridhar, Manpal;Singh, Khub
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권12호
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    • pp.1755-1760
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    • 2006
  • Studies were carried out at the National Institute of Animal Nutrition and Physiology, India to determine the effect of feeding chopped paddy straw (Oryza sativa) on the energy expenditure in crossbred cattle. Four crossbred cattle male, aged 5-6 years and weighing about 450 kg were used for this study. Three experimental trials, one each for the feeding of un-chopped paddy straw offered ad libitum (UCA), chopped paddy straw fed at restricted level (CR) and chopped paddy straw offered ad libitum (CA) were conducted. The quantity of un-chopped paddy straw consumed during UCA was assumed as the voluntary intake by the cattle and the same quantity was offered after chopping during CR. Each trial comprised of 21 d preliminary feeding period and 5 d of observation recording period. Expired gas was collected in Douglas bags using a face-mask and three-way valve at 6 hourly intervals i.e., at 09.30, 15.30, 21.30, and 03.30 h throughout the observation period. Expired gas and ambient air inspired by the animals were analyzed for the oxygen content through paramagnetic oxygen analyzer. Energy expenditure (EE) by the animals was calculated by determining the volume of oxygen consumed per minute (STP) and multiplying by 4.825. Paddy straw used in all the three trials contained (g/kg DM) 90.0 CP, 786 OM, 700 NDF, 489 ADF, 357 Cellulose and 60.0 ADL. Metabolizable energy (ME) was 6.9 MJ/kg DM. Dry matter intake (DMI) both in UCA and CR was about 6.8 kg, except that it was chopped in CR. Chopping has resulted in 32% improvement (9 kg) in DMI of CA as compared to that of UCA. Although ME intake was similar in UCA and CR (47.2 MJ/day), energy expenditure (EE) was higher in UCA (23.3 MJ) when compared to that of CR (19.5 MJ). The ME intake (63.3 MJ) as well as EE (27.1 MJ) was highest in CA. Energy expenditure when expressed as MJ/kg DMI was 3.48, 2.90 and 3.12; whereas as per cent of ME intake it was 50, 41 and 44 in UCA, CR and CA respectively. Our study has unequivocally confirmed that chopping of poor quality roughages like paddy straw has definite advantages not only in terms of improving the intake by decreasing the time taken for ingestion but also in reducing the energy cost of eating.

Nutrient Utilization, Body Composition and Lactation Performance of First Lactation Bali Cows (Bos sondaicus) on Grass-Legume Based Diets

  • Sukarini, I.A.M.;Sastradipradja, D.;Sutardi, T.;Mahardika, IG.;Budiarta, IG.A.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권12호
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    • pp.1681-1690
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    • 2000
  • A study on energy and protein utilization, and milk production of Bali cows on grass-legume diets was carried out using 12 first lactation cows (initial BW $263.79{\pm}21.66kg$) during a period of 16 weeks starting immediately post calving. The animals were randomly allotted into 4 dietary treatment groups R1, R2, R3 and R4, receiving from the last 2 months of pregnancy onwards, graded improved rations based on a mixture of locally available grass and legume feed ad libitum. R1 contained on a DM basis 70% elephant grass (PP, Penisetum purpureum) plus 30% Gliricidia sepia leaves (GS), R2 was 30% PP plus 55% GS supplemented with 15% Hibiscus tilliactus leaves (HT, defaunating effect), R3 and R4 were 22.5% PP+41.25% GS+11.25% HT+25% concentrate, where R3 was not and R4 supplemented with zinc di-acetate. TDN, CP and zinc contents of the diets were 58.2%, 12.05% and 18.3 mg/kg respectively for R1, 65.05%, 16.9% and 25.6 mg/kg respectively for R2, 66.03%, 16.71% and 29.02 mg/kg respectively for R3 and 66.03%, 16.71% and 60.47 mg/kg respectively for R4. Milk production and body weight were monitored throughout the experimental period. In vivo body composition by the urea space technique validated by the body density method and supported by carcass data was estimated at the start and termination of the experiment. Nutrient balance and rumen performance characteristics were measured during a balance trial of 7 days during the 3rd and 4th week of the lactation period. Results indicated that quality of ration caused improvement of ruminal total VFA concentration, increments being 52 to 65% for R2, R3 and R4 above R1, with increments of acetate being less (31 to 48%) and propionate being proportionally more in comparison to total VFA increments. Similarly, ammonia concentrations increased to 5.24 to 7.07 mM, equivalent to 7.34 to 9.90 mg $NH_3-N/100ml$ rumen fluid. Results also indicated that feed quality did not affect DE and ME intakes, and heat production (HP), but increased GE, UE, energy in milk and total retained energy (RE total) in body tissues and milk. Intake-, digestible- and catabolized-protein, and retained-protein in body tissues and milk (Rprot) were all elevated increasing the quality of ration. Similar results were obtained for milk yield and components with mean values reaching 2.085 kg/d (R4) versus 0.92 kg/d (R1) for milk yield, and 170.22 g/d (R4) vs 71.69 g/d (R1), 105.74 g/d (R4) vs 45.35 g/d (R1), 101.34 g/d (R4) vs 46.36 g/d (R1) for milk-fat, -protein, and -lactose, respectively. Relatively high yields of milk production was maintained longer for R4 as compared to the other treatment groups. There were no significant effects on body mass and components due to lactation. From the relationship $RE_{total}$ (MJ/d)=12.79-0.373 ME (MJ/d); (r=0.73), it was found that $ME_{m}=0.53MJ/kgW^{0.75}.d$. Requirement of energy to support the production of milk, ranging from 0.5 to 3.0 kg/d, follows the equation: Milk Prod. ($Q_{mp}$, kg/d)=[-2.48+4.31 ME($MJ/kg^{0.75}.d$)]; (r=0.6) or $Q_{mp}$=-3.4+[0.08($ME-RE_{body\;tissue}$)]MJ/d]; (r=0.94). The requirement for protein intake for maintenance ($IP_m$) equals $6.19 g/kg^{0.75}.d$ derived from the relationship RP=-47.4+0.12 IP; (r=0.74, n=9). Equation for protein requirement for lactation is $Q_{nl}$=[($Q_{mp}$)(% protein in milk)($I_{mp}$)]/100, where $Q_{nl}$ is g protein required for lactation, $Q_{mp}$ is daily milk yield, Bali cow's milk-protein content av. 5.04%, and $I_{mp}$ is metabolic increment for milk production ($ME_{lakt}/ME_{m}=1.46$).

Catalytic Supplementation of Urea-molasses on Nutritional Performance of Male Buffalo (Bubalus bubalis) Calves

  • Sahoo, A.;Elangovan, A.V.;Mehra, U.R.;Singh, U.B.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권5호
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    • pp.621-628
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    • 2004
  • Twenty male buffalo calves of 6-9 months of age (average body weight, 97 kg) were randomly allocated into two main groups of four (control) and sixteen (supplemented) calves. The supplemented group was further divided in to four equal sub-groups, with the two groups supplemented with a liquid preparation of urea-molasses, UML1, containing fish meal and UML2, containing formaldehyde treated deoiled mustard cake (FDMC) and the other two, with a semi-solid preparation, UMC1 with FDMC and deoiled rice bran (DORB) contributing similar level of CP as in UML2 and UMC2 with double the level of FDMC to that in UMC1. The control group was fed with DORB along with ad libitum wheat straw at 40:60 ratios. The rest of the groups were fed on the above diet supplemented with 500 g (as fed basis) of urea-molasses preparations. The experimental feeding was carried out for 24 weeks including a metabolism trial towards the end of experimental feeding. Daily feed intake and fortnightly change in live weight were also recorded during the study. Catalytic supplementation of 500 g urea-molasses induced 8-25% higher voluntary feed intake of wheat straw, resulting in 15-25% higher DM and OM intake. The digestibility of DM, OM, total carbohydrate, NDF, ADF, hemicellulose and cellulose in all the dietary groups were comparable. The CP digestibility of calves in supplemented groups were higher (p<0.05) than the control group. The balance of nutrients, viz. N, Ca and P, was also higher in the supplemented groups. Significantly higher intake of digestible CP coupled with other digestible nutrients attributed to higher TDN (1.67-1.78 vs. 1.37 kg) and ME (5.94-6.31 vs. 4.87 Mcal) intake in urea-molasses supplemented groups which resulted in higher live weight gain compared to that in control group (p<0.01). Between the supplements, UML2 and UMC2 faired non-significantly, indicating formalin treated mustard cake as a suitable replacement to fishmeal in the supplement. The overall ranking based on intake and digestibility of nutrients, live weight gain, economic evaluation and input-output relationship revealed that the rations with UML2 and UMC1 to be of greater value compared to other types. From the study it can be concluded that young ruminants can be reared successfully on a basal diet of deoiled rice bran and wheat straw supplemented with cheaper urea-molasses-mineral mix.

육계에 있어서 옥수수 주정박의 진정 대사 에너지가 측정 및 사료내 첨가가 생산성과 영양소 이용률에 미치는 영향 (Evaluation of True Metabolizable Energy and the Effect of Corn Distillers Dried Grains with Solubles in the Diets on Broiler Performance and Nutrient Availability)

  • 홍의철;정완태;강근호;박희두;서옥석;나재천;김원;노환국;황보종
    • 한국가금학회지
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    • 제35권4호
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    • pp.381-389
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    • 2009
  • 본 연구는 아미노산 소화율과 육계의 생산성에 대하여 CDDGS의 효과를 평가하기 위해 실시하였다. <시험 1> CDDGS의 $TME_n$가는 $2,937{\pm}50.1\;kcal/kg$ 이었다. <시험 2> 공시계는 1일령 Ross종 육계 192수를 CDDGS 첨가 수준에 따라 총 4처리구, 처리당 4반복, 반복당 12수씩 완전 임의 배치하였다. 시험 사료는 육계 초기($0{\sim}2$주), 전기($2{\sim}4$주)와 후기($4{\sim}6$)의 기본 사료에 CDDGS를 0, 6, 12, 18% 첨가하였으며, ME가와 CP는 일정하게 유지하였다. 증체량에는 큰 차이가 없었으나, 섭취량은 CDDGS 비급여구가 급여구보다 증가하였다(P<0.05). CDDGS 처리구에서 인의 소화율이 크게 증가하였으며, 아미노산 소화율은 CDDGS 첨가구가 대조구에 비해 lysine, threonine, aspartic acid 및 glycine에서 낮게 나타났다(P<0.05). 이상의 결과로부터, CDDGS는 높은 ME가를 가지고 있으며, CDDGS 첨가구는 대조구에 비해 아미노산 소화율이 낮았으나, 육계 생산성에는 크게 영향을 미치지 않았다.

Copolymerization of Ethylene and Norbornene via Polymethylene Bridged Dinuclear Constrained Geometry Catalysts

  • Zhu, Yin-Bang;Jeong, Eung-Yeong;Lee, Bae-Wook;Kim, Bong-Shik;Noh, Seok-Kyun;Lyoo, Won-Seok;Lee, Dong-Ho;Kim, Yong-Man
    • Macromolecular Research
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    • 제15권5호
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    • pp.430-436
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    • 2007
  • The dinuclear half-sandwich CGCs (constrained geometry catalyst) with a polymethylene bridge, $[Ti({\eta}^5 : {\eta}^1-indenyl)SiMe_2NCMe_3]_2(CH_2)_n]$[n = 6 (1) and 12 (2)], have been employed in the copolymerization of ethylene and norbornene (NBE). To compare the mononuclear metallocene catalysts; $Ti({\eta}^5 : {\eta}^1-2-hexylindenyl)SiMe_2NCMe_3$ (3), $(Cp^* SiMe_2NCMe_3)$Ti (Dow CGC) (4) and ansa-$Et(Ind)_2ZrCI_2$ (5), were also studied for the copolymerization of ethylene and NBE. It was found that the activity increased in the order: 1 < 2 < 3 < 5 < 4, indicating that the presence of the bridge between two the CGC units contributed to depressing the polymerization activity of the CGCs. This result strongly suggests that the implication of steric disturbance due to the presence of the bridge may playa significant role in slowing the activity. Dinuclear CGCs have been found to be very efficient for the incorporation of NBE onto the polyethylene backbone. The NBE contents in the copolymers formed ranged from 10 to 42%, depending on the polymerization conditions. Strong chemical shifts were observed at ${\delta}$42.0 and 47.8 of the isotactic alternating NBE sequences, NENEN, in the copolymers with high NBE contents. In addition, a resonance at 47.1 ppm for the sequences of the isolated NBE, EENEE, was observed in the $^{13}C-NMR$ spectra of the copolymers with low NBE contents. The absence of signals for isotactic dyad at 48.1 and 49.1 ppm illustrated there were no isotactic or microblock (NBE-NBE) sequences in the copolymers. This result indicated that the dinuclear CGCs were effective for making randomly distributed ethylene-NBE copolymers.

Utilization of Energy and Protein in Local Indian Crossb red Gilts Fed Diets Containing Different Levels of Rice Bran

  • Soren, N.M.;Bhar, R.;Chhabra, A.K.;Mandal, A.B.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권5호
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    • pp.688-692
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    • 2004
  • Fifty four crossbred gilts of 26.38${\pm}$0.85 kg body weight and 25 weeks of age were randomly divided into three groups, having six replicates of three animals each and were assigned to one of the three dietary treatments containing corn, wheat bran and rice bran (RB) at 35, 47 and 0% in $R_1$; 17.5, 23.5 and 41% in $R_2$ and 0, 0 and 82% in $R_3$. Soybean meal and fishmeal were used as source of protein at 10 and 6%, respectively, in all the rations. Average daily CP, DCP, DE and ME intake per kg $W^{0.75}$ and body weight gains were lowest (p<0.01) in gilts fed 82% RB ($R_3$), followed by $R_2$ and R1. Utilization of protein with respect to nitrogen balance and DCP conversion efficiency did not differ (p>0.05) between the treatment groups. All the gilts were in positive nitrogen balance. However, the excretion of dietary nitrogen and energy through faeces was higher (p<0.01) in gilts fed RB. However, the DE and ME conversion efficiency were higher (p<0.01) in gilts fed RB. Therefore, it can be concluded that the digestibility of energy and protein in diets containing rice bran goes on decreasing with the increasing level of fat and fibre, but the metabolizability of the energy and protein was better in pigs fed RB in the diet.

Inactivation of the genes involved in histone H3-lysine 4 methylation abates the biosynthesis of pigment azaphilone in Monascus purpureus

  • Balakrishnan, Bijinu;Lim, Yoon Ji;Suh, Jae-Won;Kwon, Hyung-Jin
    • Journal of Applied Biological Chemistry
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    • 제62권2호
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    • pp.157-165
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    • 2019
  • Di- and tri-methylation of lysine 4 on histone H3 (H3K4me2 and H3K4me3, respectively) are epigenetic markers of active genes. Complex associated with Set1 (COMPASS) mediates these H3K4 methylations. The involvement of COMPASS activity in secondary metabolite (SM) biosynthesis was first demonstrated with an Aspergillus nidulans cclA knockout mutant. The cclA knockout induced the transcription of two cryptic SM biosynthetic gene clusters, leading to the production of the cognate SM. Monascus spp. are filamentous fungi that have been used for food fermentation in eastern Asia, and the pigment Monascus azaphione (MAz) is their main SM. Monascus highly produces MAz, implying that the cognate biosynthetic genes are highly active in transcription. In the present study, we examined how COMPASS activity modulates MAz biosynthesis by inactivating Monascus purpureus cclA (Mp-cclA) and swd1 (Mp-swd1). For both ${\Delta}Mp-cclA$ and ${\Delta}Mp-swd1$, a reduction in MAz production, accompanied by an abated cell growth, was observed. Suppression of MAz production was more effective in an agar culture than in the submerged liquid culture. The fidelity of the ${\Delta}Mp-swd1$ phenotypes was verified by restoring the WT-like phenotypes in a reversion recombinant mutant, namely, trpCp: Mp-swd1, that was generated from the ${\Delta}Mp-swd1$ mutant. Real-time quantitative Polymerase chain reaction analysis indicated that the transcription of MAz biosynthetic genes was repressed in the ${\Delta}Mp-swd1$ mutant. This study demonstrated that MAz biosynthesis is under the control of COMPASS activity and that the extent of this regulation is dependent on growth conditions.

고에너지 수준의 사료급여가 돈육 품질에 미치는 영향 (Effects of High Energy Density Diet on Pork Quality)

  • 이제룡;하승호;도창희;이중동;하영주;정재두;이진우;이정일;이진희
    • 한국축산식품학회지
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    • 제24권3호
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    • pp.209-215
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    • 2004
  • 본 시험은 고에너지 수준의 사료를 급여한 돼지의 사양성적, 돈육의 도체 특성, 일반성분, 이화학적 특성 및 지방산 조성에 미치는 영향을 조사하기 위해 실시하였다. 80$\pm$3 kg의 삼원교잡종 120두를 공시하여 비육돈 사료 변화의 영양소 수준에 따라 저에너지 DE 3,290 kcal/kg, CP 14.5%, Lysine 0.70%, Ca 0.78로 배합한 사료를 38일간 급여하였고, 고에너지 DE 3,350 kcal/kg, CP 15.0%, Lysine 0.10%, Ca 0.78%로 배합한 사료를 27일간 급여하였다. 저에너지와 고에너지 사료를 급여한 돼지의 종료체중이 101 kg내외에 도달했을 때 도축하여 시험에 공시하였다. 고에너지 사료를 급여한 돼지가 일당 증체량이 높았고, 사료섭취량과 사료요구율은 낮았다(p<0.05). 도체특성과 일반성분은 차이를 보이지 않았다 이화학적 특성 중 고에너지 사료를 급여한 돈육의 가열 감량과 황색도는 저에너지 사료를 급여한 돈육에 비해 현저히 높게 나타내었다(p<0.05). 지방산 조성에서는 저에너지와 고예너지 사료를 급여한 돈육에서 차이를 보이지 않았다. 이상에서 고에너지 사료를 27일간 급여한 돼지는 저에너지 사료를 38일간 급여한 돼지에 비해 성장률이 향상되었고, 돼지의 도체 특성, 물리적 특성 및 지방산 조성에는 미치지 않았지만 돈육의 가열 감량과 황색도가 높았다.

Growth Performance, Carcass and Meat Characteristics of Black Goat Kids Fed Sesame Hulls and Prosopis juliflora Pods

  • Abdullah, Abdullah Y.;Obeidat, Belal S.;Muwalla, Marwan M.;Matarneh, Sulaiman K.;Ishmais, Majdi A. Abu
    • Asian-Australasian Journal of Animal Sciences
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    • 제24권9호
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    • pp.1217-1226
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    • 2011
  • Finding alternative feeds, such as sesame hulls and Prosopis juliflora species can attenuate difficulties of feed shortage and reduce the cost of animal feed in arid and semi-arid countries. Thirty-two Black male kids with similar initial weights (BW = $16.7{\pm}0.80\;kg$) and $120{\pm}5\;d$ of age, were used to evaluate the effect of replacing barley grains and soybean meal with Prosopis juliflora pods (PJP) and sesame hulls (SH) on growth performance, digestibility and carcass and meat characteristics. Kids were equally divided into four dietary treatment groups for an 84-d fattening period. Treatment diets had similar crude protein (CP) and metabolizable energy (ME). The treatment groups were: (T1) no PJP nor SH, (T2) 10% PJP and 20% SH, (T3) 15% PJP and 15% SH, and (T4) 20% PJP and 10% SH. A tendency was detected (p<0.08) for dry matter (DM), crude protein (CP), organic matter (OM) and neutral detergent fiber (NDF) intakes that were greater for T2 than T3 and T4 while T1 was not different from all other treatment groups. Ether extract (EE) intake was the greatest (p<0.05) for T2 and the lowest for T1. Acid detergent fiber (ADF) intake was greater (p<0.05) for T2 than T1 while T3 and T4 were intermediate. Final live weight, average daily gain (ADG) and feed conversion ratio (FCR) were comparable among different treatment groups. Digestibility of DM, OM, CP, NDF and ADF were similar among all treatment groups, however, EE digestibility was the lowest (p<0.05) for T1 when compared to other treatments. In addition, nitrogen intake, nitrogen in urine and retained and retention percentages were similar among all treatment groups. However, N loss in feces was higher (p<0.05) for T2 than T3 and T4 while T1 was intermediate. No differences were observed among treatment groups with respect to fasting live weight, hot and cold carcass weights, dressing-out percentages, mesenteric fat, visceral organs, carcass cuts percentages and carcass linear dimensions. No differences were also observed for dissected loin, leg, rack and shoulder tissues except in the total bone % for loin cuts and in the meat to bone ratio for rack cuts. T3 has the greatest total bone % and the lowest meat to bone ratio when compared to all other treatment groups. No differences were observed between treatment groups in all quality characteristics of the longissimus muscle. The present study demonstrates the potential of using PJP and SH for growing kids without adverse effects on growth performance, carcass characteristics and meat quality.