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Effects of Moisture and a Saponin-based Surfactant during Barley Processing on Growth Performance and Carcass Quality of Feedlot Steers and on In vitro Ruminal Fermentation

  • Wang, Y.;Gibb, D.;Greer, D.;McAllister, T.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1690-1698
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    • 2011
  • Feedlot and in vitro ruminal experiments were conducted to assess the effects of saponin-containing surfactant applied during tempering of barley grain on cattle growth performance and on ruminal fermentation. In the feedlot experiment, treatments with three barley grain/barley silage based diets were prepared using barley grain at 7.7% moisture (dry, D), after tempering to 18% moisture (M), or after tempering with a saponin-based surfactant included at 60 ml/t (MS). Each treatment was rolled at settings determined previously to yield optimally processed barley. A total of 180 newly weaned British${\times}$Charolais steers were fed three diets in 18 pens for a 63-d backgrounding period and 91-d finishing period to determine feed intake, growth rate and feed efficiency. Cattle were slaughtered at the end of the experiment to measure the carcass characteristics. Tempering reduced (p<0.001) volume weight and processing index, but processing characteristics were similar between MS and M. Tempering increased (p<0.05) growth during backgrounding only, compared with D, but did not affect feed intake in either phase. During backgrounding, feed efficiency was improved with tempering, but during finishing and overall this response was only observed with the surfactant. Tempering did not affect carcass weight, fat content or meat yield. Surfactant doubled the proportion of carcasses grading AAA. In the in vitro experiment, barley (500 mg; ground to <1.0 mm or steam-rolled) was incubated in buffered ruminal fluid (40 ml) without or with surfactant up to 20 ${\mu}l/g$ DM substrate for 24 h. Surfactant increased (p<0.05) apparent DM disappearance and starch digestibility but reduced productions of gas and the volatile fatty acid and acetate:propionate ratio, irrespective of barley particle size. Compared with feeding diets prepared with non-tempered barley, tempering with surfactant increased the feed efficiency of feedlot steers. This may have arisen from alteration in processing characteristics of barley grain by surfactant rather than its direct effect on rumen microbial fermentation.

Islet function within a multilayer microcapsule and efficacy of angiogenic protein delivery in an omentum pouch graft

  • McQuilling, J.P.;Pareta, R.;Sivanandane, S.;Khanna, O.;Jiang, B.;Brey, E.M.;Orlando, G.;Farney, A.C.;Opara, E.C.
    • Biomaterials and Biomechanics in Bioengineering
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    • v.1 no.1
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    • pp.27-39
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    • 2014
  • We have previously described a new multilayer alginate microcapsule system, and the goals of the present study were to assess the in vitro function of islets encapsulated in its inner layer, and the angiogenic ability of FGF-1 delivered from the external layer in an omentum pouch. Following isolation and culture, islets were encapsulated in the inner core of microspheres ($500-600{\mu}m$ in diameter) with a semi-permeable poly-L-ornithine (PLO) membrane separating two alginate layers, and both unencapsulated and encapsulated islet function was assessed by a dynamic glucose perifusion. For angiogenesis experiments, one group of microcapsules without FGF-1 (control) and another (test) containing FGF-1 with heparin encapsulated in the external layer were made. One hundred microcapsules of each group were transplanted in Lewis rats (n = 5/group) and were retrieved after 14 days for assessment of angiogenesis. Glucose perifusion of unencapsulated and encapsulated islets resulted in similar stimulation indices. The release of FGF-1 resulted in increased vascular density compared to controls. In conclusion, islets encapsulated in the core of multilayer alginate microcapsules maintain functionality and the microcapsule's external layer is effective in delivery of FGF-1 to enhance graft neovascularization in a retrievable omentum pouch.

The Role of Brain Somatostatin in the Central Regulation of Feed, Water and Salt Intake in Sheep

  • Sunagawa, Katsunori;Weisinger, Richard S.;McKinley, Michael J.;Purcell, Brett S.;Thomson, Craig;Burns, Peta L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.7
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    • pp.929-934
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    • 2001
  • The physiological role of brain somatostatin in the central regulation of feed intake in sheep was investigated through a continuous intracerebroventricular (ICV) infusion of somatostastin 1-28 (SRIF) at a small dose of $5{\mu}g/0.2ml/hr$ for 98.5 hours from day 1 to day 5. Sheep (n=5) were fed for 2 hours once a day, and water and 0.5 M NaCI solution were given ad libitum. Feed, water and salt intake were measured during ICV infusion of artificial cerebrospinal fluid (CSF) and SRIF. The feed intake during SRIF infusion on days 2 to 5 increased significantly compared to that during CSF infusion. Water intake, when compared to that during CSF infusion, only increased significantly on day 4. NaCI intake during SRIF infusion was not different from that during CSF infusion. Mean arterial blood pressure (MAP) and heart rate during SRIF infusion were not different from those during CSF infusion. The plasma concentrations of Na, K, Cl, osmolality and total protein during SRIF infusion were also not different from those values during CSF infusion.There are two possible mechanisms, that is, the suppression of brain SRIF on feed suppressing hormones and the direct actions on brain mechanisms controlling feed intake, explaining how SRIF works in the brain to bring about increases in feed intake in sheep fed on hay. The results indicate that brain SRIF increases feed intake in sheep fed on hay.

Data reduction package for the Immersion Grating Infrared Spectrograph (IGRINS)

  • Sim, Chae Kyung;Le, Huynh Anh Nguyen;Pak, Soojong;Lee, Hye-In;Kang, Wonseok;Chun, Moo-Young;Jeong, Ueejeong;Yuk, In-Soo;Kim, Kang-Min;Park, Chan;Jaffe, Daniel T.;Pavel, Michael
    • The Bulletin of The Korean Astronomical Society
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    • v.38 no.2
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    • pp.84.1-84.1
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    • 2013
  • We present a python-based data reduction pipeline for the Immersion GRating INfrared Spectrograph (IGRINS). IGRINS covers the complete H- and K-bands in a single exposure with a spectral resolving power of greater than 40,000. IGRINS is designed to be compatible with telescopes of diameters ranging from 2.7-m (the Harlan J. Smith telescope at McDonald Observatory) to 8-10m. Commissioning and initial operation will be on the 2.7-m telescope from late 2013. The pipeline package is a part of the IGRINS software and designed to be compatible with other package that maneuvers the spectrograph during the observation. This package provides high-quality spectra with minimal human intervention and the processes of order extraction, distortion correction, and wavelength calibration can be automatically carried out using the predefined functions (e.g. echellogram mapping and 2D transform). Since the IGRINS is a prototype of the Giant Magellan Telescope Near-Infrared Spectrometer (GMTNIRS), this pipeline will be extended to the GMTNIRS software.

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A Study on Gas-Liquid Contact in a Perforated Plate-Type $SO_2$ Absorber at Flooding Conditions

  • Han, Seung-Ho;Soowoo Kwon;Sangwon Jung;Jaehyuk Junk;Yang, Chang-Ryung;Carl Weilert
    • Journal of Korean Society for Atmospheric Environment
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    • v.15 no.E
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    • pp.17-28
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    • 1999
  • Gas-liquid contact tests above a perforated-plate were conducted with air and water at flooding gas-flow conditions in order to study two-phase flow characteristics in a limestone-gypsum SO2 absorber. Gas layers were in the form of air pockets and confined to the limited areas around each duct pipe, while the remaining tary area were in the wet condition. The liquid above the tray was always in the flooded and even fluidized conditions at gas flows over the range studied, although vigorous bubbly or churn-turbulent two-phase regime was only observed in the immediate vicinity of the gas hole exit at low gas loads. The froth zone was extremely active to provide intimate contact between gas and liquid so that the necessary mass transfer operation can take place, which is the primary purpose of high-performance SO2 absorbers. Howefer, the absorber $\Delta$P was 250mmH2O for the initial water level at 150mm, which is an important issue to be resolved for economical operation of the SO2 absorber. It was seen in the liquid level-and gas flow-transient tests that changes in the absorber liquid inventory were much more pronounced for intimate gas-liquid contact than changes in the gas flow. Based on the 4- and 8-duct pipe test results, grouping the duct pipes near the center of the test tray seemed to promote better recirulation of liquid from gas-liquid contact zone back to the reaction tank so that the absorbed SO2 can be neutralized.

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ASSESSING CALIBRATION ROBUSTNESS FOR INTACT FRUIT

  • Guthrie, John A.;Walsh, Kerry B.
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1154-1154
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    • 2001
  • Near infra-red (NIR) spectroscopy has been used for the non-invasive assessment of intact fruit for eating quality attributes such as total soluble solids (TSS) content. However, little information is available in the literature with respect to the robustness of such calibration models validated against independent populations (however, see Peiris et al. 1998 and Guthrie et al. 1998). Many studies report ‘prediction’ statistics in which the calibration and prediction sets are subsets of the same population (e. g. a three year calibration validated against a set from the same population, Peiris et al. 1998; calibration and validation subsets of the same initial population, Guthrie and Walsh 1997 and McGlone and Kawano 1998). In this study, a calibration was developed across 84 melon fruit (R$^2$= 0.86$^{\circ}$Brix, SECV = 0.38$^{\circ}$Brix), which predicted well on fruit excluded from the calibration set but taken from the same population (n = 24, SEP = 0.38$^{\circ}$Brix with 0.1$^{\circ}$Brix bias), relative to an independent group (same variety and farm but different harvest date) (n = 24, SEP= 0.66$^{\circ}$ Brix with 0.1$^{\circ}$Brix bias). Prediction on a different variety, different growing district and time was worse (n = 24, SEP = 1.2$^{\circ}$Brix with 0.9$^{\circ}$Brix bias). Using an ‘in-line’ unit based on a silicon diode array spectrometer, as described in Walsh et al. (2000), we collected spectra from fruit populations covering different varieties, growing districts and time. The calibration procedure was optimized in terms of spectral window, derivative function and scatter correction. Performance of a calibration across new populations of fruit (different varieties, growing districts and harvest date) is reported. Various calibration sample selection techniques (primarily based on Mahalanobis distances), were trialled to structure the calibration population to improve robustness of prediction on independent sets. Optimization of calibration population structure (using the ISI protocols of neighbourhood and global distances) resulted in the elimination of over 50% of the initial data set. The use of the ISI Local Calibration routine was also investigated.

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Bale Location Effects on Nutritive Value and Fermentation Characteristics of Annual Ryegrass Bale Stored in In-line Wrapping Silage

  • Han, K.J.;McCormick, M.E.;Derouen, S.M.;Blouin, D.C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.9
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    • pp.1276-1284
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    • 2014
  • In southeastern regions of the US, herbage systems are primarily based on grazing or hay feeding with low nutritive value warm-season perennial grasses. Nutritious herbage such as annual ryegrass (Lolium multiflorum Lam.) may be more suitable for preserving as baleage for winter feeding even with more intensive production inputs. Emerging in-line wrapped baleage storage systems featuring rapid wrapping and low polyethylene film requirements need to be tested for consistency of storing nutritive value of a range of annual ryegrass herbage. A ryegrass storage trial was conducted with 24-h wilted 'Marshall' annual ryegrass harvested at booting, heading and anthesis stages using three replicated in-line wrapped tubes containing ten round bales per tube. After a six-month storage period, nutritive value changes and fermentation end products differed significantly by harvest stage but not by bale location. Although wilted annual ryegrass exhibited a restricted fermentation across harvest stages characterized by high pH and low fermentation end product concentrations, butyric acid concentrations were less than 1 g/kg dry matter, and lactic acid was the major organic acid in the bales. Mold coverage and bale aroma did not differ substantially with harvest stage or bale location. Booting and heading stage-harvested ryegrass baleage were superior in nutritive value to anthesis stage-harvested herbage. Based on the investigated nutritive value and fermentation characteristics, individual bale location within in-line tubes did not significantly affect preservation quality of ryegrass round bale silages.

Large eddy simulation of flow over a wooded building complex

  • Rehm, R.G.;McGrattan, K.B.;Baum, H.R.
    • Wind and Structures
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    • v.5 no.2_3_4
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    • pp.291-300
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    • 2002
  • An efficient large eddy simulation algorithm is used to compute surface pressure distributions on an eleven story (target) building on the NIST campus. Local meteorology, neighboring buildings, topography and large vegetation (trees) all play an important part in determining the flows and therefore the pressures experienced by the target. The wind profile imposed at the upstream surface of the computational domain follows a power law with an exponent representing a suburban terrain. This profile accounts for the flow retardation due to friction from the surface of the earth, but does not include fluctuations that would naturally occur in this flow. The effect of neighboring buildings on the time dependent surface pressures experienced by the target is examined. Comparison of the pressure fluctuations on the single target building alone with those on the target building in situ show that, owing to vortices shed by the upstream buildings, fluctuations are larger when such buildings are present. Even when buildings are lateral to or behind the target, the pressure disturbances generate significantly different flows around this building. A simple grid-free mathematical model of a tree is presented in which the trunk and the branches are each represented by a collection of spherical particles strung together like beads on a string. The drag from the tree, determined as the sum of the drags of the component particles, produces an oscillatory, spreading wake of slower fluid, suggesting that the behavior of trees as wind breakers can be modeled usefully.

The Role of Corticotropin-Releasing Factor and Urocortin in Brain Mechanisms Controlling Feed Intake of Sheep

  • Sunagawa, K.;Weisiger, R.S.;McKinley, M.J.;Purcell, B.S.;Thomson, C.;Burns, P.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.11
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    • pp.1529-1535
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    • 2000
  • The aim of the present study was to determine whether brain corticotropin-releasing factor (CRF) and a new peptide, urocortin (UCN) have a direct action in brain mechanisms controlling feed, water and salt intake in sheep. We gave a continuous intracerebroventricular (ICV) infusion of the peptide at a small dose of $5{\mu}g/0.2ml/hr$ for 98.5 hrs from day 1 to day 5 in sheep not exposed to stress. Feed and water intake during ICV infusion of CRF or UCN decreased significantly compared to those during artificial cerebrospinal fluid (CSF) infusion. NaCl intake during infusion of CRF or UCN was the same as that during CSF infusion. Mean carotid arterial blood pressure (MAP) and heart rate during ICV infusion of CRF or UCN were not significantly different from that during CSF infusion. On the other hand, the plasma glucose concentration during ICV infusion of CRF or UCN tended to be higher than that during CSF infusion. These observations indicate that decreased feed intake induced by CRF and UCN infusion is not mediated by the activation of both the pituitary-adrenal axis and the sympathetic nervous system. The results suggested that brain CRF and UCN act directly in brain mechanisms controlling ingestive behavior to decrease feed and water intake, but do not alter salt intake in sheep.

Detector Mount Design for IGRINS

  • Oh, Jae Sok;Park, Chan;Cha, Sang-Mok;Yuk, In-Soo;Park, Kwijong;Kim, Kang-Min;Chun, Moo-Young;Ko, Kyeongyeon;Oh, Heeyoung;Jeong, Ueejeong;Nah, Jakyoung;Lee, Hanshin;Jaffe, Daniel T.
    • Journal of Astronomy and Space Sciences
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    • v.31 no.2
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    • pp.177-186
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    • 2014
  • The Immersion Grating Infrared Spectrometer (IGRINS) is a near-infrared wide-band high-resolution spectrograph jointly developed by the Korea Astronomy and Space Science Institute and the University of Texas at Austin. IGRINS employs three HAWAII-2RG Focal Plane Array (H2RG FPA) detectors. We present the design and fabrication of the detector mount for the H2RG detector. The detector mount consists of a detector housing, an ASIC housing, a Field Flattener Lens (FFL) mount, and a support base frame. The detector and the ASIC housing should be kept at 65 K and the support base frame at 130 K. Therefore they are thermally isolated by the support made of GFRP material. The detector mount is designed so that it has features of fine adjusting the position of the detector surface in the optical axis and of fine adjusting yaw and pitch angles in order to utilize as an optical system alignment compensator. We optimized the structural stability and thermal characteristics of the mount design using computer-aided 3D modeling and finite element analysis. Based on the structural and thermal analysis, the designed detector mount meets an optical stability tolerance and system thermal requirements. Actual detector mount fabricated based on the design has been installed into the IGRINS cryostat and successfully passed a vacuum test and a cold test.