• Journal of Microbiology and Biotechnology
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• v.30 no.5
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• pp.668-680
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• 2020

Bacillus velezensis is an important plant growth-promoting rhizobacterium with immense potential in agriculture development. In the present study, Bacillus velezensis Lle-9 was isolated from the bulbs of Lilium leucanthum. The isolated strain showed antifungal activities against plant pathogens like Botryosphaeria dothidea, Fusarium oxysporum, Botrytis cinerea and Fusarium fujikuroi. The highest percentage of growth inhibition i.e., 68.56±2.35% was observed against Fusarium oxysporum followed by 63.12 ± 2.83%, 61.67 ± 3.39% and 55.82 ± 2.76% against Botrytis cinerea, Botryosphaeria dothidea, and Fusarium fujikuroi, respectively. The ethyl acetate fraction revealed a number of bioactive compounds and several were identified as antimicrobial agents such as diketopiperazines, cyclo-peptides, linear peptides, latrunculin A, 5α-hydroxy-6-ketocholesterol, (R)-S-lactoylglutathione, triamterene, rubiadin, moxifloxacin, 9-hydroxy-5Z,7E,11Z,14Z-eicosatetraenoic acid, D-erythro-C18-Sphingosine, citrinin, and 2-arachidonoyllysophosphatidylcholine. The presence of these antimicrobial compounds in the bacterial culture might have contributed to the antifungal activities of the isolated B. velezensis Lle-9. The strain showed plant growth-promoting traits such as production of organic acids, ACC deaminase, indole-3-acetic acid (IAA), siderophores, and nitrogen fixation and phosphate solubilization. IAA production was accelerated with application of exogenous tryptophan concentrations in the medium. Further, the lily plants upon inoculation with Lle-9 exhibited improved vegetative growth, more flowering shoots and longer roots than control plants under greenhouse condition. The isolated B. velezensis strain Lle-9 possessed broad-spectrum antifungal activities and multiple plant growth-promoting traits and thus may play an important role in promoting sustainable agriculture. This strain could be developed and applied in field experiments in order to promote plant growth and control disease pathogens.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.17 no.2
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• pp.48-54
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• 2017

Purpose: Fukuyama congenital muscular dystrophy (FCMD) is a rare, autosomal-recessive disorder characterized by early-onset hypotonia associated with brain malformations in dystroglycanopathy. Although the wide spectrum of congenital muscular dystrophies causes difficulty in diagnosis, correlating the genotype with the clinical phenotype can help diagnose FCMD. Here, we evaluated the correlation of targeted molecular genetic analysis of FKTN gene mutation with the FCMD phenotype. Methods: This study was conducted retrospectively with 9 subjects. Inclusion criteria included clinical symptoms characterized by early-onset hypotonia with magnetic resonance imaging (MRI) featuring brain malformations. FKTN gene-alteration analysis was performed using various FKTN gene-analysis methods, including sequencing. Results: Among the 9 subjects studied, 4 (44.4%) were male and 5 (55.6%) were female. The median age of onset of the first symptom was 3.1 months. The first symptom was a delayed milestone in 6 cases (66.7%). All 9 subjects (100%) presented with early-onset hypotonia and global delayed development. All subjects presented with cortical malformation in their brain MRIs. Of the 9 subjects, 6 subjects had previously undergone muscle biopsy and 4 cases (4/6; 66.7%) showed dystrophic or myopathic features. Pathogenic mutations causing FCMD were identified in 3 cases. Conclusions: In this study, all 3 subjects with FKTN mutations showed important MRI findings (pachygyria and cerebellar dysplasia). These data suggest that patients with characteristic phenotypes who show pachygyria and cerebellar abnormalities in brain MRIs may have a high probability of being diagnosed with FCMD.

• Korean Journal of Environmental Agriculture
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• v.9 no.2
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• pp.133-141
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• 1990

For the purpose of evaluating nutrient loadings into rivers and lakes from agricultural land, especially from paddy fields and also nutrient degradation in drainage channels, the Total Kjeldahl Nitrogen(TKN) and the Total Phosphorus(TP) were investigated in 29.5 ha. paddy fields in Hwa-Sung, Kyong-Ki, Korea, during the period from May 8, 1989 to Sep. 27, 1989. The results of the study can be su㎜arized as follows : 1. Annual inputs into paddy fields were 180 N-kg/ha 46 P-kg/ha. by fertilization, and 15.0 TKN-kg/ha. 10.0 TP-kg/ha. by irrigation, 8.0 TKN-kg/ha. 0.34 TP-kg/ha. by rainfall respectively. The amount of nutrient involved in surface runoff from paddies was 39.0 TKN-kg/ha. 9.2 TP-kg/ha. and in seepage 7.5 TKN-kg/ha. 2.1 TP-kg/ha. respectively 2. In WS1 stream(reach length equals 950m), nutrients decreased 0.31 TKN-mg/L/km, 0.01 TP-mg/L/km and in WS2 stream (reach length equals 750m) which are more meandering and undulating than WS1, the nutrients decreased 0.84 TKN-mg/L/km, 0.11 TP-mg/L/km. From these results, it was concluded that low stream velocity due to meandering and undulation promotes more degradation of nutrient concentrations. 3. For the purpose of decreasing nutrient loads from paddy fields, the amount of fertilizer used needs to be controlled, irrigation weirs need to be constructed in the drainage channels to delay the transportation of nutrients by decelerating the stream velocity and plants such as plantain-lily need to be cultivated in the channel to consume nutrients and therefore enlarge chances of self-purification.