• Proceedings of the Plant Resources Society of Korea Conference
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• v.18 no.1
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• pp.41-51
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• 2005

We examined whether extracts from 14 local citrus spp. on Jeju Island (Korea) contained chemosensitizing activity that would increase the cytotoxic effect of vincristine(VCR) in drug-resistant cancer cells. We report that methanol extracts from fruits and flowers of some species had a chemosensitizing effect that reversed P-glycoprotein (Pgp)-mediated multidrug resistance (MDR). Using drug-sensitive AML-2/WT and drug-resistant AML-2/D100 in the absence of VCR in human acute myelogenous leukemia cells we found that fruit or flower extracts alone generally had low cytotoxicity $(IC_{50}>200\;{\mu}g/ml)$. In studies examining the effect of extracts on 120 ng/ml VCR cytotoxicity in drug-resistant AML-2/D100 cells, we found that immature fruit extracts had greater chemosensitizing activity than either extracts from mature fruit or flower. Of the 14 species examined, the immature fruit extract from Inchangkyool (Citrus ichangiensis) showed the hishest chemosensitizing index(CI) valus. Immature fruit extracts of Hongkyool(C. tachibana), Byungkyool(C. platymamma), Cheongkyool(C. nippokoreana) and Jinkyool (C. sunki) also strongly potentiated VCR cytotoxicity in AML-2/D100 cells. The chemosensitizing effect of peel extracts was 2-10-fold that of whole fruit extracts from Hongkyool (C. tachibana), Byungkyool (C. platymamma) and Inchangkyool (C. inchangiensis). The CI values for flower extracts were higher than those for mature fruit extracts, but lower than those for immature fruit extracts. These results indicate that immature citrus fruits contain compounds that do not exert their activity solely through cytotoxicity. In particular, Incahngkyool (C. inchangiensis), Byungkyool(C.platymamma), Cheongkyool(C. nippokoreana) and Hongkyool (C. tahibana) may be useful sources of chemosensitizing compounds.

• Horticultural Science & Technology
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• v.29 no.5
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• pp.494-499
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• 2011

Cold pretreatment, washing medium and composition of nutrient media may have marked effects on microspore embryogenesis. When microspores isolated from radish (Raphanus sativus L. cv. Gwanhun) flower buds were washed with Nitsch & Nitsch (NLN) medium liquid medium containing $130g{\cdot}L^{-1}$ sucrose (NLN-13), yields of microspore-derived embryos were greater than when using B5 liquid medium containing $130g{\cdot}L^{-1}$ sucrose. Microspore viability is known to decrease rapidly with storage; however, in this experiment, microspore viability was maintained for 24 h at $4^{\circ}C$ without media. Among the various medium concentrations used ($0.25{\times}$, $0.5{\times}$, $1.0{\times}$, $2.0{\times}$, and $4.0{\times}$ NLN liquid medium), $0.5{\times}$ NLN liquid medium induced the most efficient formation of microspore-derived embryos. In addition, microspore-derived embryos yields were greater when microspores were cultured in $0.5{\times}$ NLN liquid medium supplemented with $0.25{\times}$, $0.5{\times}$, and $1.0{\times}$ NLN microelements, compared to medium not supplemented with microelements. In this study, the highest yield of microspore-derived embryos was observed when the microspores derived from flower buds were washed using NLN-13 liquid medium and then cultured on $0.5{\times}$ NLN liquid medium supplemented with $0.25{\times}$ NLN microelements, followed by incubation at $25^{\circ}C$ for 30 days.

• Journal of the Korean Society of Food Culture
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• v.26 no.1
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• pp.39-52
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• 2011

This study examined the types and cooking methods for dasik (traditional pressed sweet), as recorded in 16 old Joseon dynasty (1392-1909) studies. The ingredients used in dasik during the Joseon dynasty were categorized into cereal powders, tree fruits, flower powders, root clods, dry-fish beef powders, and vegetables. In the early, middle, and late eras of the Joseon dynasty, $1^{st}$ set (two, five, and 70 kinds of dasik), $2^{nd}$ set (two, four, and 16 kinds of dasik) were prepared using cereal powders, and during the middle and late eras of the Joseon dynasty one and 22 kinds of dasik were prepared using tree fruits. During the late eras of the Joseon dynasty, seven kinds of dasik were prepared using flower powders, 11 kinds of dasik were prepared using root clods, 12 kinds of dasik were prepared using dry-fish beef powders, and two kinds of dasik were prepared using vegetables. The frequency of the ingredients were in the order of Huek-im (黑荏), Hwang-yul (黃栗), Jin-mal (眞末), Song-wha (松花), and Nok-mal (菉末) during the Joseon dynasty. To prepare dasik, the ingredients were mixed with honey, soy sauce, sesame oil, pepper, and water or ground and shredded to prepare for pressing and for abstract dasik, respectively. The appearance and taste of dasik varied, thereby resulting in nutrient supplementation, as the types of ingredients increased throughout the Joseon dynasty. This observation may be associated with the commercial industrial development that prevailed during the late Joseon dynasty. Further investigation will be conducted on the recipes and ingredients recorded in these old studies to develop a standardized recipe for the globalization of dasik.

• BMB Reports
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• v.34 no.3
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• pp.201-205
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• 2001

Thionins are a family of low molecular weight cysteine-rich antimicrobial peptides. We isolated a cDNA encoding thionin gene from a flower bud cDNA library of Chinese cabbage (CFT). The gene contains 611 by nucleotides with 60 bp, and 150 by untranslated regions at its N- and C-terminal, respectively. The deduced amino acid sequence encoded 133 amino acids containing precursor polypeptide. The protein reveals that the precursor has a tripartite structure: a putative signal sequence at the N-terminus, followed by a mature thionin peptide, and a C-terminal acidic domain, which facilitates transport of the mature thionin through membrane. Genomic Southern blot analysis suggests that the CFT gene may be present as a single or two copy gene in the Chinese cabbage genome. Northern blot analysis shows that the gene is specifically expressed in flowers, but not in leaves, stems, or roots. When we analyzed the antifungal activity of the recombinant CFT protein, which was expressed in E. coli using the truncated cDNA region corresponding to the mature protein part, it was not active on fungal growth inhibition.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.6
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• pp.705-711
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• 2016

Crimson clover, a legume crop, is a landscape crop and green manure crop that can be sowing in spring and autumn. Its red flower blooms in May, and serves various roles such as landscape composition, weeds suppressing, prevention of soil loss and nutrient on sloping land and supplying nitrogen and organic matter in soil. Thus, in order to utilize this crop in agriculture land, we evaluated the growth characteristics of crimson clover cultivated in four different soil textures; sand, sandy loam, loam, and clay loam. The nitrogen content of crimson clover was $15.8g\;kg^{-1}$ and C/N ratio was 20.3. Its plant height was 42.5 cm in sandy loam and 49.5 cm in loamy, respectively, approximately 20 cm longer than the sand and clay loam. The crimson clover in sandy loam and loam bloomed about seven days earlier than those in sand and clay loam. Regarding number of flower per hill and flower length, there were no difference among the soil textures. Dry weight of crimson clover for sandy loam and loam was $2.5Mg\;ha^{-1}$ and $2.3Mg\;ha^{-1}$, respectively, $0.8{\sim}1.1Mg\;ha^{-1}$ higher than that of sand and sandy loam. Plant height and dry weight of crimson clover increased with delaying harvest time. Nitrogen contribution in loam and clay loam was $51.3kg\;ha^{-1}$ and $53.5kg\;ha^{-1}$, respectively. Therefore, in terms of flowering properties and dry weight, the proper soil texture for the growth and development of crimson clover was sandy loam and loam.

• Korean Journal of Medicinal Crop Science
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• v.2 no.2
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• pp.146-148
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• 1994

The most important subject in cultivation of A. gigas NAKAI would be how to decrease the bolting rate. Belcause the decrease and poor of the yields and quality is imevitable in such field where the bolting rate is high. This study was carried out to investigate the time of flower-bud differentiation as a part of improvements of cultivation method. Shoot apex was fixed in Farmer's solution(ETOH 3:Acetic acid 1), thin paraffin ribbon was made, and the tissue was stained by Safranin solution. Differentiation time of flower-bud in A. gigas differed greatly from individuals by the seedling size, generally begun from middle of May to late of June, it was time that two leaves spread. In this results, Photoperiocid types of A. gigas NAKAI were regarded as LI type or LS type of dull-photosensitivity.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.367-367
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• 2017

Crimson clover, a legume crop, is a landscape crop and green manure crop that can be sowing in spring and autumn. Its red flower blooms in May, and serves various roles such as landscape composition, weeds suppressing, prevention of soil loss and nutrient on sloping land and supplying nitrogen and organic matter in soil. Thus, in order to utilize this crop in agriculture land, we evaluated the growth characteristics of crimson clover cultivated in four different soil textures, sand, sandy loam, loam, and clay loam. The nitrogen content of crimson clover was 15.8 g kg-1 and C/N rate was 20.3. Its growth was good in sandy loam and loam. Its plant height was 42.5 cm in sandy loam and 49.5 cm in loamy, respectively, which are approximately 20 cm longer than the sand and clay loam. The crimson clover in sandy loam and loam bloomed about seven days earlier than those in sand and clay loam. Regarding number of flower per hill and flower length, there were no difference between soil textures. Dry weight of crimson clover was 2.5 Mg ha-1, 2.3 Mg ha-1 each in sandy loam and loam. Therefore, it was approximately 0.8 ~ 1.1 Mg ha-1 higher than dry weight of sand and sandy loam. Plant height and dry weight of crimson clover was increased late harvest time. Nitrogen contribution were higher in loam and clay loam, when it was respectively 51.3 kg ha-1, 53.5 kg ha-1. Therefore, according to flowering properties and dry weight, the growth and development of crimson clover was finest in sandy loam and loam.

• Korean Journal of Plant Resources
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• v.28 no.3
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• pp.297-304
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• 2015

The flower buds of Sophora japonica L (SF), as a well-known traditional Chinese medicinal herb, have been used to treat bleeding-related disorders such as hematochezia, hemorrhoidal bleeding, dysfunctional uterine bleeding, and diarrhea. However, no specific anti-cancer effect and its molecular mechanism of SF have been described. Thus, we performed in vitro study to investigate if treatment of SF affects activating transcription factor 3 (ATF3) expression and ATF3-mediated apoptosis in human colorectal cancer cells. The effects of SF on cell viability and apoptosis were measured by MTT assay and Western blot analysis against cleaved poly (ADP-ribose) polymerase (PARP). ATF3 activation induced by SF was evaluated using Western blot analysis, RT-PCR and ATF3 promoter assay. SF treatment caused decrease of cell viability and increase of apoptosis in a dose-dependent manner in HCT116 and SW480 cells. Exposure of SF activated the levels of ATF3 protein and mRNA via transcriptional regulation in HCT116 and SW480 cells. Inhibition of extracellular signal-regulated kinases (ERK) 1/2 by PD98059 and p38 by SB203580 attenuated SF-induced ATF3 expression and transcriptional activation. Ectopic ATF3 overexpression accelerated SF-induced cleavage of PARP. These findings suggest that SF-mediated apoptosis may be the result of ATF3 expression through ERK1/2 and p38-mediated transcriptional activation.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.143-148
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• 2015

A new Dianthus cultivar 'URI 2010-6' was selected among F1 progenies from a cross between interspecific hybrid 'Evergreen Emerald' as the seed parent and D. chinensis ' Ruby' as the pollen parent. In 2007, the cross w as made, and the seedlings of $F_1$ progenies were obtained and investigated. After three rounds of characteristic evaluation from 2008 to 2010, a clone was selected and registered as 'URI 2010-6' at the Korea Seed and Variety Service (plant variety protection number: 4431) in 2011. The flower of 'URI 2010-6' is caryophyllaceous, single type, and mainly bright red (Red, N79C) with secondary white (White, N155C) on the edge or as spots on the petal. The flower has diameter of 2.7 cm and length of 0.8 cm. This cultivar blooms continuously about 150 d on average and may be used as evergreen plant during winter months.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.247-254
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• 2008

This study was conducted to investigate effect of temperature, photoperiod, and plant growth regulators on the growth and flowering of Dendrobium phalaenopsis. Photoperiod treatments of 10 or 16 day length were from Mar. 24 to May 23. After treatments, all plants were exposed under natural photoperiod. Temperature treatments promoted development of new shoots (leads) of Dendrobium phalaenopsis 'Semi Alba' at $30/25^{\circ}C$(day/night) for 12 weeks, and 16 hr-photoperiod treatment promoted development of new shoots and flowering. The 16 hr photoperiod Dendrobium phalaenopsis 'Candistraipe ${\times}$ Tedtakiguz' no difference in the numbers of flower, flower width, and stalk length compared to the 10 hr photoperiod. Days to first flowering from appearance of new shoot were 241 days under natural photoperiod, 243 days under 10 hr, and 216 days under 16 hr, so that 16 hr-photoperiod treatment was faster about 25 days than that of natural or 10 hr treatment. There was not significant difference in growing and flowering of Dendrobium phalaenopsis among plant growth regulators.