• Biotechnology and Bioprocess Engineering:BBE
• /
• v.10 no.4
• /
• pp.296-303
• /
• 2005

A strain named as HJ35 was isolated from the skin of sixty-five men and fourteen women for acne therapy, in order to find an effective antimicrobial agent against Propionibacterium acnes. Isolate HJ35 was identified as Enterococcus faecium based on 16 rDNA sequence and produced enterocin HJ35 having antimicrobial activities against most lactic acid bacteria, En­terococcus spp., Staphylococcus aureus, S. epidermidis, Clostridium perfringens, some bacilli, Mi­crococcus flavus, Listeria monocytogenes, L. ivanovii, Escherichia coli, Pseudomonas fluorescens and Propionibacterium acnes, in the modified well diffusion method. Especially, enterocin HJ35 showed a bactericidal activity against Propionibacterium acnes P1. The antimicrobial activity of enterocin HJ35 was disappeared completely with the use of protease XIV. But enterocin HJ35 activity is very stable at high temperature (up to $100^{\circ}C$ for 30 min), in wide range of pH (3.0${\~}$9.0), and by treatment with organic solvents. The apparent molecular mass of enterocin HJ35 was estimated to be approximately 4${\~}$4.5 kDa on detection of its bactericidal activity after SDS-PAGE. In batch fermentation of E. faecium HJ35, enterocin HJ35 was produced at the mid­log growth phase, and its maximum production was obtained up to 2,300 AU/mL at the late stationary phase. By employing fed-batch fermentation, the enhanced production of enterocin HJ35 was achieved up to 12,800 AU/mL by feeding with 10 g/L glucose or 6 g/L lactate.

• BMB Reports
• /
• v.30 no.2
• /
• pp.101-105
• /
• 1997

Extracellular phospholipase $A_2$ activity has been detected in urine of patients with acute pyelonephritis (APN). This enzyme required micromolar $Ca^{2+}$ ion for its maximum activity and showed a broad range of pH (4.5~10) optimum. Urine enzyme hydrolyzed phosphatidylethanolamine (PE) and phosphatidylserine (PS) more effectively than phosphatidylcholine (PC). $PLA_2$ activity in the urine of patients with APN was about 5-fold higher than that of healthy individuals. When urine was subjected to heparinSepharose column chromatography, phospholipase $A_2$ activity was detected in both heparin-non-binding and binding fractions. Both phospholipase $A_2$ activities were sensitive less than a micromolar calcium concentration and did not react with anti-human 14-kDa group II phospholipase $A_2$ monoclonal antibody, HP-l. These findings suggest that two kinds of novel extracellular phospholipase $A_2$. which may not belong to the 14-kDa group II phospholipase $A_2$ family, exist in the urine of patients with APN.

• Smart Structures and Systems
• /
• v.3 no.3
• /
• pp.341-356
• /
• 2007

A good understanding of normal modal variability of civil structures due to varying environmental conditions such as temperature and wind is important for reliable performance of vibration-based damage detection methods. This paper addresses the quantification of wind-induced modal variability of a cable-stayed bridge making use of one-year monitoring data. In order to discriminate the wind-induced modal variability from the temperature-induced modal variability, the one-year monitoring data are divided into two sets: the first set includes the data obtained under weak wind conditions (hourly-average wind speed less than 2 m/s) during all four seasons, and the second set includes the data obtained under both weak and strong (typhoon) wind conditions during the summer only. The measured modal frequencies and temperatures of the bridge obtained from the first set of data are used to formulate temperature-frequency correlation models by means of artificial neural network technique. Before the second set of data is utilized to quantify the wind-induced modal variability, the effect of temperature on the measured modal frequencies is first eliminated by normalizing these modal frequencies to a reference temperature with the use of the temperature-frequency correlation models. Then the wind-induced modal variability is quantitatively evaluated by correlating the normalized modal frequencies for each mode with the wind speed measurement data. It is revealed that in contrast to the dependence of modal frequencies on temperature, there is no explicit correlation between the modal frequencies and wind intensity. For most of the measured modes, the modal frequencies exhibit a slightly increasing trend with the increase of wind speed in statistical sense. The relative variation of the modal frequencies arising from wind effect (with the maximum hourly-average wind speed up to 17.6 m/s) is estimated to range from 1.61% to 7.87% for the measured 8 modes of the bridge, being notably less than the modal variability caused by temperature effect.

• The Journal of the Acoustical Society of Korea
• /
• v.25 no.3E
• /
• pp.115-122
• /
• 2006

The bistatic scattering of an incident wave by a hemi-spherically capped cylinder is of particular interest because it has rarely been studied until the present day. The configuration of a hemi-spherically capped cylinder is similar to naval underwater weapons (submarines, mines, torpedos, etc.), but which is not exactly the same. This paper describes a novel laboratory experiment aimed at direct measurement of bistatic scattering by a hemi-spherically capped cylinder. Bistatic scattering by a hemi-spherically capped cylinder was measured in an acoustic water tank (5m long, 5m wide, 5m deep) using a high frequency projector (120kHz) and hydrophone. Measurements of monostatic scattering were also made under the same conditions. The bistatic scattering pattern by a hemi-spherically capped cylinder was measured against the incident angles $(0^{\circ},\;15^{\circ},\;20^{\circ},\;30^{\circ},\;45^{\circ},\;60^{\circ},\;90^{\circ})$ in order to verify various scattering pattern characteristics by the change of incident angle. The results indicate that the bistatic scattering TS at a wide scattering angle is much stronger than the mono static scattering TS. In bistatic scattering, the forward scattering TS is significantly stronger than the backward scattering TS, and the forward scattering pattern is also broader. In case of seven incident angles, the maximum value of forward scattering TS is about 14dB stronger than that of backward scattering TS. It is also found that forward scattering varies with the incident angle of sound to a much less extent than backscattering, and it is not seriously affected by the incident angle. These features could be the advantages of using forward scattering for detecting underwater targets at long range and increasing detection area and probability.

• Journal of the Korea Society of Computer and Information
• /
• v.17 no.11
• /
• pp.33-38
• /
• 2012

An free-fall object is received only force of gravity. Movement that only accept gravity is free-fall movement, and a free-falling object is free falling body. In other words, free falling body is only freely falling objects under the influence of gravity, regardless of the initial state of objects movement. In this paper, we assume, ignoring the resistance of the air, and the free-fall acceleration by the height does not change within the range of the short distance in the vertical direction. Under these assumptions, we can know about time and maximum height to reach the peak point from jumping vertically upward direction, time and speed of the car return to the starting position, and time and speed when the car fall to the ground. It can be measured by jumping degree and risk of accident from car or motorcycle in telematics.

• Journal of the Korean Chemical Society
• /
• v.38 no.1
• /
• pp.50-54
• /
• 1994

Spectrophotometric determination of some light lanthanide ions by flow injection method is described. Chromeazurol S forms water soluble complex with lanthanide ions in the presence of DTAB. The absorption maximum of the complexes are from 650 nm to 655 nm and the molar absorptivities were ca. $1.8{\times}10^5\;L mol^{-1}cm^{-1}$ on Tris buffer (pH 10.5). The calibration curves for Nd(III), Eu(III) and Sm(III) obtained by FIA are over the range of 0.1 to 0.6 ppm and the correlation coefficient were ca. 0.9993. The detection limits (S/N) were from 10 ppb for Nd(III) and Eu(III) to 20 ppb for Sm(III). The relative standard deviations was ${\pm}$.2% for 0.4 ppm sample. The samples throughput was ca. $50\;cm^{-1}$.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.29 no.4
• /
• pp.308-315
• /
• 2018

In this paper, we present the basic design results for high-resolution radar development at S-band frequency that can precisely measure the miss distance between two targets. The basic system requirement is proposed for the design of a 3.5 GHz linear frequency-modulated (LFM) radar with maximum detection distance and distance resolution of 2 km and 1 m, respectively, and the specifications of each module are determined using the radar equation. Our calculations revealed a signal-to-noise ratio ${\geq}30dB$ with a bandwidth of 150 MHz, transmission power of 43 dBm for the power amplifier, gain of 26 dBi for the antenna, noise figure of 8 dB, and radar cross-section of $1m^2$ at a target distance of 2 km from the radar. Based on the calculation results and the theory and method of LFM radar design, the hardware was designed using software defined radar technology. The results of the subsequent field test are presented that prove that the designed radar system satisfies the requirements.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.34 no.2
• /
• pp.135-138
• /
• 1998

This paper describes the construction and experimetal results of prototype digital telesounder developed to monitor remotely the fish school entered in setnet. The telesounder consists of the sea station and land station. The former consists of an ultrasonic transceiving part of 50 KHz, a hand made 16 bits single board computer (SBC), and a RF MODEM of 9600 bps for data link. The SBC controls the trigger signal to transmit the ultrasonic pulse, maximum detection range, sampling rate in analog to digital converter and data link. The sampling rate of echo data was 80 ${\mu}$s. The later consists of a RF MODEM same as the former one and 486 notebook computer which displays the echogram and saves its raw data. The authors carried out some experiments to confirm the practical use of the telesounder. At results the echogram obtained on the land station is sufficiently good and clear to monitor the behavior of fish school in setnet.

• Journal of Environmental Health Sciences
• /
• v.40 no.3
• /
• pp.178-186
• /
• 2014

Background: This study was conducted to assess health risks in regard to exposure by children to volatile organic compounds (VOCs) in children's products. Methods: Ten VOCs were measured by head-space gas chromatography in children's products, including toys, oil pastels, sign pens, furniture, ball pools, and playmats. We estimated the average daily dose (ADD) via inhalation during the use of these children's products and calculated hazard quotient (HQ) by dividing ADD by reference dose of VOCs. Results: Among the measured VOCs, five compounds were identified in children's products: benzene, ethylbenzene, styrene, toluene, and xylene. The detection rates of VOCs in toys, ball pools, furniture, playmats, sign pens, and oil pastels were 85%, 100%, 100%, 30%, 100%, and 60%, respectively. The maximum levels of VOCs were 0.18 mg benzene/kg in toys, 5.92 mg toluene/kg in playmats, 10.37 mg ethylbenzene/kg in ball pools, 24.85 mg xylene/kg in toys, and 118.29 mg styrene/kg in ball pools. From exposure levels of VOCs in the children's products HQs were calculated within a range of $5.71{\times}10^{-10}$ to $4.77{\times}10^{-4}$. The HQ of xylene was the highest for children aged 0-6 playing on the playmats. However, the HQ via inhalation exposure to VOCs in individual products did not exceed 1.00. Conclusion: Based on the results, it was concluded that the use of these children's products do not pose health risks to children.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.31 no.3A
• /
• pp.274-281
• /
• 2006

Sensor network applications need synchronized time extremely such as object tracking, consistent state updates, duplicate detection, and temporal order delivery. This paper describes reliable time synchronization protocol (RTSP) for wireless sensor networks. In the proposed method, synchronization error is decreased by creating hierarchical tree with lower depth and reliability is improved by maintaining and updating information of candidate parent nodes. The RTSP reduces recovery time and communication overheads comparing to TPSN when there are topology changes owing to moving of nodes, running out of energy and physical crashes. Simulation results show that RTSP has about 20% better performance than TPSN in synchronization accuracy. And the number of message in the RTSP is $20%{\sim}60%$ lower than that in the TPSN when nodes are failed in the network. In case of different transmission range of nodes, the communication overhead in the RTSP is reduced up to 40% than that in the TPSN at the maximum.