• Journal of the Korean Society of Tobacco Science
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• v.21 no.1
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• pp.10-16
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• 1999

Leaves harvested were separated with visual characters into 2 classes such as immature and mature leaves. In the curing process, the prolonged yellowing treatment during yellowing stage was automatically controlled at the different stalk position, and condition of curing process after this period was all the same with conventional ones. In case of prolonged yellowing in immature leaves, increase of price per kg reached to 8 % compared with those of conventional ones. In physical properties, filling capacity and shatter index was decreased with the degree of maturity, and it was equal level in filling capacity of immature leaves between curing method, while shatter index was decreased in prolonged yellowing treatment than that of conventional ones. There was no difference in chemical components between immature leaves of prolonged yellowing and conventional ones. As to the prolonged yellowing of immature leaves, there was decreased in citric and malic acid contents of the nonvolatile organic acids, and it was equal level in all higher fatty acids content of leaves cured by prolonged yellowing treatment compared with in that of conventional curing method. The contents of key compounds such as solanone, damascenone, damascone in the essential oil were lower in prolonged yellowing of immature leaves than those of mature leaves cured by conventional ones.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.200-206
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• 1996

Experiment was conducted to get the information about physico-chemical properties of flue-cured tobacco on the degree of maturity cultivated in paddy-upland rotated field, and compared to upland ones. For the samples of this experiment, 3~4 leaves at each stalk position were harvested from the bottom of plants. Physico-chemical properties of cured leaves were determined from the samples collected at weekly intervals, and of obtained from 4 stalk positions. For the degree of maturity, harvested leaves were separated with visual characters into four classes such as immature, mature, ripe, and mellow. Regardless of stalk position, the order of shrinkage rate with length and width of leaves was mellow> immature> ripe> mature, and ripe leaves from paddy field showed higher shrinkage rate than those of upland. Nicotine and total nitrogen contents were decreased with the degree of maturity while reducing sugar content were showed a reverse tendency. Ripe leaves from paddy field had lower reducing sugar contents, comparing with upland tobacco. Filling capacity of cured leaves from paddy field was decreased with degree of maturity, but there was no difference between upland and paddy tobacco. Shatter index was increased in the oeder of immature > mellow > mature > ripe. Chemical components of cigarette smoke from paddy field tobacco were little higher in $CO_2$ total particulate matter and tar contents, while combustibility was little lower than that of upland tobacco. It was also evaluated that paddy field tobacco was unfavorable for the non-volatile organic and higher fatty acids contents comparing with upland tobacco.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.152-155
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• 2003

Burcucumber (Sicyos angulatus L.), used as a medicinal or rootstock for cucurbitaceous crops have shown high variation in seed maturity and size. This study was carried out to determine the effect of seed maturity and size on seed germination, seedling emergence and growth of burcucumber, Seeds collected from a native site were divided into small, medium and large ones after sorted to mature and immature ones. Their germination, seedling emergence and growth were done in indoor and a green house. Mature seeds showed higher germination and seedling emergence rate than immature ones. Regardless of their maturity, medium. and small seeds showed higher germination rate than large ones. Medium and large seeds, however, had the greatest and the least seedling emergence, respectively. Seedling height, number of true leaves areas of cotyledons and true leaves except hypocotyl length were increased with increased seed size although were not affected by the maturity. Cotyledon, leaf, hypocotyl, root and their total dry weights were greater in large mature seeds than large immature ones while increased with increased seed size in both maturities.

• Korean Journal of Plant Resources
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• v.9 no.3
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• pp.274-278
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• 1996

This study was conducted to examine the dry matter production and leaf photosynthesis under different fertilizer application in Yacon(Polymnia sonchifolia)plants. Under field conditions, dry matter production of stable mature application plot(S-1) was much larger than non-treated control plot(CTL), but bulb dry weight of 10kg nitrogen application plot per 10a (N-10) was not differentiated from CTL. The maximum photosynthetic rates increased in Yacon plants grown at S-1 while in the rates of CTL reduced under glasshouse in the same treated pot condition. The optimum temperature for the highly photosynthetic rates is about $34{\pm}3^{\circ}C$, and the rates did decrease by stomatal resistance below 28℃. The estimated light saturation point was $1200{\mu}mol{\cdot}m^{-2}s^1$. The results of this study indicate that soil conditions were highly related to dry matter production and leaf photosynthesis rate in Yacon plants.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.153-160
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• 2004

Glehnia littoralis is known as an edible and medicinal plant using green loaves and mature roots of plant. In the present paper, the influence of plant growth regulators on callus induction and plant regeneration was investigated. Callus induction and regeneration occurred from leaf and petiole explants in Glehnia littoralis. Optimal condition of plant growth regulators for callus induction from leaf and petiole explants was MS basal medium supplemented with 2mg/L 2,4-D and 2mg/L BA. The frequency of callus induction was higher in petiole explant than leaf. When the callus was cultured on MS basal medium supplemented with 0∼1 mg/L IAA, 0∼1mg/L NAA and 0∼2mg/L BA for about 65 days, the most effective plant growth regulators on plant regeneration from callus were 1mg/L NAA and 2mg/L BA. The plantlets acclimatized successfully and grown in vermiculite matrix.

• Plant Biotechnology Reports
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• v.3 no.3
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• pp.191-197
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• 2009

Four-leaf white clover is not found easily in nature due to its low appearance rate (1 in 10,000). Because people believe that it brings good luck and like to either keep it or present it to a loved one, it has commercial and ornamental value. To breed four-leaf clover, we exposed its flowers to ${\gamma}-rays$ at the pollination stage. The $M_1$ seeds produced following doses at 25-100 Gy showed an approximately 74% germination rate, with seedling survival at 46%. In the $M_1$ generation of plants irradiated within that dose range, we found an increased frequency of four leaflets. One of them, Jeju Lucky-1 (JL-1), had a frequency of about 60%. To see whether that mutation was somaclonal or genetic, we observed its $M_2$ generation and found that such a phenotype reappeared. Although our results demonstrated that the irradiation of fully mature flowers led to a higher frequency of 4-leaflets, we could not clearly explain the genetic mechanism involved. We suggest that JL-1 is valuable as a new variety, without further genetic fixation, because white clover can be propagated vegetatively by stolons.

• Korean Journal of Medicinal Crop Science
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• v.12 no.3
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• pp.183-190
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• 2004

The major compositions of leaf tea and flower tea were investigated to develope as a new functional tea using Korean native Camellia japonica L. Most of leaf teas, except flower tea, were considered as good materials with basic conditions for tea manufacture because water content was below 6%. Crude protein was the greatest component in roasted young leaf tea (RYLT), crude fats in roasted mature leaf tea (RMLT) and ashes in fermented young leaf tea (FYLT). Caffein were present as the highest amount (5.18%) in steamed mature leaf tea (SMLT), showing less amount than green tea. Catechin were contained as the highest amount in all kinds of teas, especially FYLT was the highest (9.57%). Tannin, which highly related with tea quality including astringent taste, color and perfume, were present as the highest amount in FYLT. Vitamin C was highly detected in the tea from flowers (22.7 mg/l00 g) rather than in the tea from leaves. The content of theanine were found in flower tea by 1,074 mg/l00 g, and had about twofold of FYLT and RYLT. Among free amino acids, glutamic acid and aspartic acid were higher detected in SMLT and RMLT while asparagine was present as higher amounts in RYLT and FYLT, expecting these components can improve tea taste. Nucleic acids and their derivatives including GMP, hypoxanthine and AMP were detected as the higher amounts by 7.86, 8.57, and $12.67\;{\mu}mol/g$, respectively, however IMP content was even reduced by all manufacturing processes. In all kinds of tea, sugars such as glucose, fructose, sucrose and maltose were detected, specially glucose and fructose were found as highest amount in RFT by 65.5 and 59.6 nmol/0.1 mg, respectively.

• Research in Plant Disease
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• v.9 no.3
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• pp.159-161
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• 2003

Leaf spot diseases were found on safflower (Carthamus thinctorius) leaf in several fields located in Euisong-Gun, Gyengbuk province in Korea. The infected leaf rate of the diseases in surveyed area were different each years from 2001 to 2003 such as about 5%, 10%, and 30%, repectively. The typicla symptoms of diseases appeared first as small, light brown spots on leaves. And then spot turn dark brown and magnified. And yellow decoloration zone appeared surrounding dark brown spots. Mycological characteristics of Alternaria carthami, isolated from safflower mature leaf spot were dark gray colonies, conidiophores simple erect, septated, 40~80 ${\mu}m$ length; conidia solitary, straight, body size fo without beak length 40~100${\mu}m$, 10~15${\mu}m$ thick, Number of transverse septa 4~10 and longitudinal septa 4~7 ; beak with 2~4 septa, 30~65${\mu}m$ length. And A. alternata, isolated from old leaf were gray~dark gray colonies, conidiophores simple or branched, 20~110 ${\mu}m$ length; conidia long chaines, short conical or cylindrical beak, 2~6 transverse septa and several longitudinal septa, body size of without beak length 30~60 ${\mu}m$, 10~20 ${\mu}m$ thick ; beak length 5~35 ${\mu}m$. These are the first report on the leaf spot of safflower caused by Alternaria carthami, A alternata in Korea.

• Korean Journal of Plant Tissue Culture
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• v.24 no.3
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• pp.129-135
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• 1997

Morphogenetic responses were investigated by culturing embryo and leaf explants of Korean wild type tea plant, Camellia sinensis (L.) O. Kuntze. Induction of direct somatic embryogenesis as well as adventitious and/or axillary shoots was obtained from mature zygotic embryo cultures on Murashige and Skoog (MS) basal medium having 5 to $20\mu\textrm{M}$cytokinin a lone. Morphogenetic response was decreased dramatically by the addition of auxins tested. One hundred percent of induced and isolated shoots formed roots after four weeks of culture on half-strength MS or quarter-strength Schenk and Hildebrandt (SH) media supplemented with $10\mu\textrm{M}$indole-3-butyric acid (IBA). Immature zygotic embryos were shown to be a suitable explant for embryogenic callus formation in the presence of 2, 4-dichlorophenoxyacetic acid(2, 4-D) in basal medium. Mature zygotic embryo originated leaves were used to test their ability for mophogenesis by incorporating plant growth regulators such as IBA, naphthyl-1-acetic acid (NAA), and 6-benzylaminopurine (BAP). Apparently, the morphogenetic responses of the cultured explant sources on the types and/or levels of plant growth regulators tested were observed visually.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.3
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• pp.184-187
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• 2004

In case of a semi-forcing culture for vegetable perilla, the following research results on the effect of black polyethylene mulching, the optimum sowing season, and the seedling stand improvement method were drawn. Soil temperature was higher and the emergence was faster in a black polyethylene mulching culture than in a non-mulching culture. However, the mature was late, the main stem were larger, and the seed yield, as well as the leaf yield, was greater in a mulching culture than in a non-mulching culture. Considering growth and chilling injury, the stable sowing season of vegetable perilla was judged to be the early in January. The covering materials for improvement of the seedling stand can be a non-woven fabric and hyaline polyethylene. However, the non-woven fabric seemed to be quite suitable in view of the stability and convenience of control after the emergence of perilla.