• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.5
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• pp.526-535
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• 2001

The gametogenesis, reproductive cycle, frequency distribution of egg diameter and fecundity of spotted sea bass Lateolabrax maculatus were investigated by histological observation. The samples were collected at Goheung, Jeonam from December 1996 to November 1997. Gonadosomatic index (CSI) was appeared less than 1.0 in female and male from December to July and began to increase from August and reached the maximum values of female 7.50 and male 2.98 in October. Hepatosomatic index (HSI) was appeared annual maximum value of female 1.97 and male 1.64 in March and then decreased from April. The annual reproductive cycle of this species can be divided into four successive stages: growing stage (July to August), maturation stage (September to October), ripe and spawning stage (October to November) and degeneration and resting stage (November to July). According to the frequency distribution of egg diameter, the development mode of egg was not seperated, formed one mode for the maturation and spawning season, The individual absolute fecundity ranges from $1,726,242\~17,822,576$ eggs, and individual relative fecundity ranges from $29,458\~214,730$ eggs in total length (cm) and $938\~3,363$ eggs in body weight (g). Female and male of L. maculatus were matured when total length were over 50.1 and 45.1 cm, respectively.

• Journal of Fisheries and Marine Sciences Education
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• v.24 no.6
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• pp.1003-1012
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• 2012

The gonadosomatic index (GSI), sex steroid hormones and gonadal development of roughscale sole Clidoderma asperrimum cultured in indoor tank were investigated to evaluate its sexual maturation and reproductive cycle. The highest GSI values of female and male were $6.91{\pm}4.03$ (May) and $0.16{\pm}0.08$ (August), respectively. The reproductive cycle would be classified into four successive developmental stages: growing stage (December to February), maturation stage (March to April), ripe and spawning stage (May to June), recovery and resting stage (July to November). The highest plasma testosterone (T) and estradiol-$17{\beta}$ ($E_2$) levels of female were $259.4{\pm}76.8$ and $633.3{\pm}182.5$ pg/mL, respectively in May. Also $17{\alpha}$, $20{\beta}$-dihydroxy-4-pregen-3-one ($17{\alpha}$, $20{\beta}$-OHP) levels of female peaked in April before spawning season ($244.2{\pm}42.5$ pg/mL). The highest plasma testosterone (T) and 11-ketotestosterone levels of male were $231.0{\pm}46.0$ and $273.9{\pm}54.5$ pg/mL, respectively in April. But there was no significant difference in $17{\alpha}$, $20{\beta}$-OHP.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.1
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• pp.41-47
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• 2009

The seasonal reproductive cycle of Gomphina melanaegis collected in the coastal area of Jumunjin, between April 2006 and March 2007, was analyzed. Maturation cycle parameters such as the gonad index (GI), ovarian egg diameter, frequency of developmental stages, protein content, and RNA/DNA variation in the gonads were analyzed monthly for the 40 samples. According to the indices from histological sections, the frequency of gonad developmental stages, and the oocyte diameter, this clam has a long-term partial spawning pattern from March to October. However, GI and nucleic acid values showed that the mature stage is from March to July and that the main spawning season is August. The peak RNA and DNA contents were good indicators of sexual maturation in females and males, respectively. The variation in protein content corresponded with the RNA/DNA ratios.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.3 no.1
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• pp.19-26
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• 1970

During the summer of 1969, a series of experiments on the spawning and development of the eggs of grass carp and silver carp was performed with the following results, but the complete development of eggs Iras not accomplished: 1. The season of maturity for both species is estimated to be from the end of June to the beginning of August. 2. Pituitary glands for the use of inducing maturation should be obtained before the maturation season of its donor. 3. Silver carp as small as 40 cm in body length (48 cm in total length) with an age of 6 years were found to have reached maturity, and the number of eggs in the ovaries were from about 23 to 26 thousand for those with body lengths of 40-44.5 cm. 4. The conical-shaped net cloth incubator worked better than others, and each incubator was most effective when installed in parallel series to the water supply pipe with a valve for each incubator to control water flow. 5. During the egg development, if any cell or cells were displaced, the eggs eventually died. 6. The proper manipulation of brood fish before the egg-taking seems to be very Important, and close care should be paid to feeding them well and keeping them in a pond of proper water depth.

• The Korean Journal of Malacology
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• v.13 no.1
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• pp.43-54
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• 1997

1994년 1월부터 12월까지 1년간에 걸쳐 우리 나라 서해안 비인만의 조간대에서 채집된 떡조개, Dosinorbis(Phacosoma) japonocus(Reeve)를 대상으로 성숙 및 산란시기를 규명하기 위해 생식소 여포지수(GFI)및 생식소지수(GI)의 월별변화, 생식주기, 월별 난경 조성 및 성비를 조사하였다. 본 종은 자웅이체이고, 생식소는 중장선 하부와 소화맹낭 그리고 섬유성 결체조직과 근섬유에 의해 치밀하게 구성된 외측 근섬유층 사이에 위치하고 있다. 산란기 추정을 위해 조사된 GFI와 GI의 월별변화 양상은 생식주기와 일치하는 경향을 보였다. 난경 55-65$\mu\textrm{m}$인 성숙란들은 젤라틴성 막에 의해 둘러싸여 있다. 본 종의 생식주기는 초기활성기 (12-2월), 후기활성기(2-5월), 성숙기(5-8월), 부분산란기(6-8월)그리고 퇴화 및 비활성기(8-12월)의 연속적인 5단계로 나눌 수 있었다. 산란기는 수온 및 먹이이용 가능성과 밀접하게 관련되어 있는 것으로 보이며, 산란은 수온이 2$0^{\circ}C$ 이상인 6월부커 8월까지 일어났고, 산란성기는 6월과 7월이었다. 산란기는 단지 그 해 하계에만 일어남으로써 1년에 1회의 산란기를 가지나 그 산란 빈도 횟수는 1년에 1회의 산란계절 중 2회 이상 일어나는 것으로 추정된다. 암.수 개체들의 성비는 통계 처리한 결과 1:1(x$^{2}$=0.08, P>0.05)로 나타나 암.수간 성비에 차이가 없었다.

• Journal of Aquaculture
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• v.13 no.1
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• pp.47-53
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• 2000

Hormone induction in maturation and ovulation was carried out with spotted halibut (Verasper variegatus) which normally does not spawn spontaneously in captivity. Prior to spawning females were treated with human chorionic gonadotropin(HCG 265-678 IU/kg BM) 17 ${\alpha}$-hydroxy 20-${\beta}$-dihydroprogesterone (17 ${\alpha}$ 20${\beta}$ OHP 0.5-1.0${\mu}$g/kg BW) and luteinizing hormone-releasing hormone analogue pellet (LHRHa pellet 63-81 ${\mu}$g/kg BW) In all the trials HCG injection succeeded in inducing ovulation at 265-300 IU/kg BW. HCG at 620 or 678 IU/kg BW was ineffective in inducing a good rate of ovulation. Final maturation and ovulation were not stimulated by treatment of 17 ${\alpha}$ 20${\beta}$OHP and LHRHa pellet. These results suggest that HCG injection (around 300 IU/kg BW) is more effective compared with LHRHa pellet implantation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.1
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• pp.41-47
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• 2017

Perhaps the most common type of reproductive dysfunction in captive fish is failure of females to undergo final oocyte maturation and thus to ovulate and spawn. The success of aquaculture could therefore be improved by developing techniques to enhance natural spawning, artificial maturation, and/or to induce ovulation in farmed fish. This study aimed to investigate the effects of prostaglandin $E_2$ ($PGE_2$) and prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$) on in vitro oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation in the marine fish Chasmichthys dolichognathus. Post-vitellogenic follicles (0.80-0.94 mm diameter oocytes) were incubated with $PGE_2$ or $PGF_{2{\alpha}}$ at concentrations of 5, 50, or 500 ng/mL for 24 hours. A significant increase in GVBD was seen in 0.84 mm and 0.94 mm oocytes incubated with 50 ng/mL $PGE_2$ compared with the control. There was no significant increase in GVBD in any of the other experimental conditions (5 or 500 ng/mL $PGE_2$ or 5, 50, or 500 ng/mL $PGF_{2{\alpha}}$). Neither of the prostaglandins induced ovulation at the concentrations tested.These results suggest that GVBD was induced by incubation with 50 ng/mL $PGE_2$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.6
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• pp.523-528
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• 1984

The small filefish, Rudarius ercodes, generally spawning from mid May to early October in the natural habitat, was exposed to various photoperiod and temperature regimes. These environmental effects on the gonad activity, regression and recrudescence were experimentally investigated based on the mechanism of reproductive cycle. Spawning season was initiated in the early spring with the gonad activated by long photoperiod(13L) and stimulated by compensatory temperature rising. Even when the gonad activated readily at the above critical daylength (12L to 13L)was kept back at the below if, it went on maturing. At the end of spawning period (mid September), since the shortening of daylength (12L) resulted in the gonad regression regardless of temperature, the short daylength might be related to the termination of spawning in situ. When the regressive gonad at the post spawning period was treated by the above 13L: $20^{\circ}C$ condition, it could recrudesce and bring forth even spawning. From this fact, the feasible control of annual reproductive cycle of small filefish was recognized. But even in the long daylength, the temperature above $28^{\circ}C$ was preventive of gonad maturation.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2005.07a
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• pp.52-52
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• 2005

Oogenesis, the gonadosomatic index (GSI), reproductive cycle and first sexual maturation of the female Neptunea (Barbitonia) arthritica cumingii have been investigated by light and electron microscope observations. In the early vitellogenic oocyte, the Golgi complex and mitochondria were involved in the formation of glycogen, lipid droplets and yolk granules. In late vitellogenic oocytes, the rough endoplasmic reticulum and multivesicular bodies were involved in the formation of proteid yolk granules in the cytoplasm. In particular, compared with the results of other gastropods, it is a different result that appearances of cortical granules at the cortical layer and microvilli on the vitelline envelope, which is associated with heterosynthetic vitellogenesis, were not observed in vitellogenic oocytes during oogenesis. A mature yolk granule was composed of three components: main body (central core), superficial layer, and the limiting menbrane, Monthly changes in the gonadosomatic index in females were studied in 2002 and 2003 were closely associated with ovarian developmental phases. Spawning occurred between May and August in 2002 and 2003 and the main spawning occurred between June and July when the seawater temperature rose to approximately 18${\sim}$23${\circ}$C. The female reproductive cycle can be classified into five successive stages: early activestage (Septmber to October), late active stage ( November to February), ripe stage (February to June), partially spawned stage (May to Aygust), and recovery stage (June to August).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.1
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• pp.76-90
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• 1993

To clarify the relations between photoperiodism and x-organ of eyestalks to the control of gonadal maturation in a freshwater prawn, Macrobrachium nipponense, the present investigation was performed with following examinations: 1) the influence of water temperature and daylength on the control of gonadal maturation, 2) the effect of eyestalk ablation on the gonadal maturation, 3) the seasonal variation of histology of MEX-organ (medulla externa X-organ) in the eyestalk. In previtellogenesis period (December${\sim}$March) and vitellogenesis period (April${\sim}$May), gonadal maturation was considerablely influnced by water temperature. In these periods, GSI increased and gonads were matured with water temperature rising without regard to photoperiod conditions (12L/12D and 15L/9D). In spawning period (June${\sim}$August), however, gonadal maturation was influenced by photoperiod condition. While high value of GSI was kept at long photoperiod regime (15L/9D), GSI was decreased at short photoperiod regime (12L/12D). In resting period (September${\sim}$November), no rematuration was occurred at all the experimental regimes combinated with water temperatures ($16^{\circ}C,\;22^{\circ}C,\;28^{\circ}C$) and photoperiod (12L/12D, 15L/9D). Effect of X-organ which inhibite the gonadal maturation was stronger in resting period than that in previtellogenesis and ealy spawning periods by observations on the effectiveness of eyestalk ablation on the gonadal maturation. In MEX-organ of eyestalk, the number of neurosecretory cells of which size was over $20{\mu}m$ in diameter varied according to the reproductive cycle. The number of cells increased $77{\pm}12$ in resting period, and decreased $55{\pm}7$ in vitellogenesis period. Volume of Bellonci's organ, however, increased in vitellogenesis period in comparison with that in resting period.