• Journal of the Korean Chemical Society
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• v.54 no.1
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• pp.38-42
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• 2010

A sensitive quantitative method for the determination of matrine alkaloids in human urine was developed based on hollow fiber liquid-phase microextraction (HF-LPME) combined with HPLC. The influence of the different factors on the HF-LPME efficiency including the pH and ion strength of the donar solution, the pH of the acceptor solution, stirring rate and extraction time were examined. The best HF-LPME conditions were as follows: 1-octanol impregnated in the pores of the hollow fiber, 100 mmol/L of $H_3PO_4$ at pH 1.50 as the acceptor solution injected into the lumen of the hollow fiber, 1 mol/L NaOH used to adjust the pH of the donor solution, stirring rate of 600 rpm and extraction time of 60 min. The LPME method was applied successfully to the analysis of matrine and sophocarpine in real urine samples.

• Korean Journal of Environmental Agriculture
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• v.34 no.1
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• pp.1-5
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• 2015

BACKGROUND: The stabilities of the two alkaloidal insecticides of S. flavescens including matrine and oxymatrine are important factor to establish expiry date and usage manual for crop protection. However, the environmental stability of the compounds had not been studied with the extract and its commercial biopesticide. METHODS AND RESULTS: The environmental stabilities of the two alkaloids were performed with extract of S. flavescens, and its two commercial biopesticides both in controlled aquatic and soil conditions. The half-lives of the total matrines for the extract and its two commercial biopesticides were estimated over 200 days both under aerobic and anaerobic water condition. Under dry soil condition, the initial decay rates of the matrines were calculated 0.0804-0.1275 ($t_{1/2}$ 5.4-8.6 days), and the half-lives under wet soil condition were calculated 33.0-231 days. Total soil bacteria on the wet soil ranged 6.0-8.0 log CFU/g-soil during the experiments period. CONCLUSION: The aquatic mixture of the extract showed excellent stability both with the extract and its biopesticides, however, the stability of soil mixture were shorter than the aquatic mixture, suggesting that soil metal consider as a catalyst for the degradation of the two alkaloids.