• Title/Summary/Keyword: Matrices

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Environmental Occurrence of Persistent Organochlorines in Gwangyang Bay (광양만내 지속성유기염소계화합물의 잔류농도 및 분포특성)

  • 홍상희;임운혁;심원준;오재룡
    • Korean Journal of Environmental Biology
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    • v.22
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    • pp.30-37
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    • 2004
  • Peysistent oyganochlorine compounds (OCs) weve determined in sediments and bivalves from Gwangyang Bay. The concentrations of ∑PCB, ∑DDT, ∑HCH and ∑CHL in sediments were in the range of f 2.25∼11.4 ng g$\^$-1/, 0.16∼1.16 ng g$\^$-1/, nd∼0.51 ng g$\^$-1/, and 0.05∼0.79 ng g$\^$-1/, respectively. The overall OCs concentrations in sediments were below the effect range -median (ER-M) values toy benthic organisms suggested by NOAA (1991). Levels of PCB compounds a re relatively lower than other industrialized bays (Pusan Bay, Ulsan Bay, and Youngil Bay). OCs accumulated in bivalves were higher than those in sediments. In bivalves, the concentration ranges of ∑PCB, ∑DDT, ∑HCH and ∑CHL were 9.97∼31.7 ng g$\^$-1/, 7.54∼22.6 ng g$\^$-1/, 0.49∼2.0 ng g$\^$-1/, and 0.82∼7.32 ng g$\^$-1/, respectively. Relatively high PCB concentrations in both environmental matrices are found at the inner bay than the outer part, indicating that the sources of PCBs were located inside the bay. DDT compound showed relatively high concentrations in the vicinity of the mouth of river and urban area, whereas other organochlorine pesticides show homogeneous distributions over the bay. Homologue profile of PCB compounds shows that low-chlorinated congeners (especially, di-, tyi- and tetra-) are abundant in Gwangynng Bay, which is diferent from other areas in Korea.

Nonlinear Flexural Modeling of Prestressed Concrete Beams with Composite Materials (복합소재 프리스트레스트 콘크리트보의 비선형 휨 모델링)

  • ;;Naaman, Antoine
    • Magazine of the Korea Concrete Institute
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    • v.10 no.6
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    • pp.269-280
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    • 1998
  • Recently, application of composite materials such as fiber reinforced concretes(FRCs) and fiber reinforced plastics(FRPs) in conjunction with conventional structural components has become one of the main research areas. A proper use of advanced composite materials requires understanding their resistance mechanism and failure mode when they are applied to structures or their components. Particular considerations are given in this research to develop an analytical model which can predict the nonlinear flexural responses of bonded and unbonded prestressed concrete beams possibly having layers of different cementitious composite matrices in a section and/or FRP tendons. The block concept is used, which can be regarded as an intermediate modeling method between the couple method with one block and the layered method with multiply sliced layers in a section. In order to find a particular deflection point of a beam under load, solutions to the 2N-variables are found numerically by using approximate N-force equilibrium equations and N-moment equilibirum equations. The model is shown to successfully predict the flexual behavior of variously reinforced bonded and unbonded prestressed concrete beams. The model is also successful in simulating a gradually increasing load after sudden drop inload resistance due to fracture of one or more FRP tendons. This feature is useful in tracing the overall load-deflection response of a beam prestressed with brittle FRP tendons.

Identification and molecular characterization of the chitinase gene, EaChi, from the midgut of the earthworm, Eisenia andrei (붉은줄지렁이 (Eisenia andrei) 중장에서 발현되는 chitinase 유전자, EaChi의 동정 및 분자생물학적 특성에 관한 연구)

  • Tak, Eun Sik;Kim, Dae hwan;Lee, Myung Sik;Ahn, Chi Hyun;Park, Soon Cheol
    • Journal of the Korea Organic Resources Recycling Association
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    • v.18 no.3
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    • pp.31-37
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    • 2010
  • Chitinases (EC 3.2.1.14) hydrolyze the ${\beta}$-1,4-linkages in chitin, the second most abundant polymer of N-acetyl-${\beta}$-D-glucosamine which is a structural component of protective biological matrices such as fungal cell walls and insect exoskeletons. The glycosyl hydrolases 18 family including chitinases is an ancient gene family widely expressed in archea, prokaryotes and eukaryotes. Since earthworms live in the soil with a lot of microbial activities and fungi are supposed to be a major component of the diet of earthworm, it has been reported that there would be appropriate immune system to protect themselves from microorganisms attacks. In this study, the novel chitinase, EaChi, from the midgut of earthworm, Eisenia andrei, were identified and characterized. To obtain full-length cDNA sequence of chitinase, RT-PCR and RACE-PCR analyses were carried out by using the previously identified EST sequence amongst cDNA library established from the midgut of E. andrei. EaChi, a partial chitinase gene, was composed of 927 nucleotides encoding 309 amino acids. By the multiple sequence alignments of amino acids with other different species, it was revealed that EaCHI is a member of glycosyl hydrolases 18 family, which has two highly conserved domains, substrate binding and catalytic domain.

Three Dimensional Target Volume Reconstruction from Multiple Projection Images (다중투사영상을 이용한 표적체적의 3차원 재구성)

  • 정광호;진호상;이형구;최보영;서태석
    • Progress in Medical Physics
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    • v.14 no.3
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    • pp.167-174
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    • 2003
  • In the radiation treatment planning (RTP) process, especially for stereotactic radiosurgery (SRS), knowing the exact volume and shape and the precise position of a lesion is very important. Sometimes X-ray projection images, such as angiograms, become the best choice for lesion identification. However, while the exact target position can be acquired by bi-projection images, 3D target reconstruction from bi-projection images is considered to be impossible. The aim of this study was to reconstruct the 3D target volume from multiple projection images. It was assumed that we knew the exact target position in advance, and all processes were performed in Target Coordinates, where the origin was the center of the target. We used six projections: two projections were used to make a Reconstruction Box and four projections were for image acquisition. The Reconstruction Box was made up of voxels of 3D matrices. Projection images were transformed into 3D in this virtual box using a geometric back-projection method. The resolution and the accuracy of the reconstructed target volume were dependent on the target size. An algorithm was applied to an ellipsoid model and a horseshoe-shaped model. Projection images were created geometrically using C program language, and reconstruction was also performed using C program language and Matlab ver. 6(The Mathwork Inc., USA). For the ellipsoid model, the reconstructed volume was slightly overestimated, but the target shape and position proved to be correct. For the horseshoe-shaped model, reconstructed volume was somewhat different from the original target model, but there was a considerable improvement in determining the target volume.

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Simulation of Separating Isoclinics and Isochromatics from Photoelastic Fringes of a Disk using 8-step Phase Shifting Methodology (광탄성 프린지 위상이동법을 적용한 디스크의 등경 및 등색프린지 분리법에 관한 시뮬레이션)

  • Baek, Tae-Hyun;Kim, Myung-Soo;Cho, Sung-Ho
    • Journal of the Korean Society for Nondestructive Testing
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    • v.21 no.2
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    • pp.189-196
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    • 2001
  • Photoelasticity is one of the most widely used methods for whole field stress analysis. In photoelasticity, the difference and the directions of the principal stresses we given isochromatic and isoclinic fringe patterns. Conventionally, principal stress directions are measured manually by relating the polarizer and analyzer of a plane polariscope at the same time. This is known to be the Tardy compensation method. This measurement can be very tedious and time consuming in whole field analysis. It is not possible to separate isoclincs from photoelastic fringes by conventional photoelastic technique. In this study, photoelastic theory is represented by Jones matrices and 4-steps and 8-steps phase shifting methods are described A feasibility study using computer simulation is done to separate isoclincs and isochomatics from photoelastic fringes of a circular disk under diametrical compression. Fringe patterns of the disk are generated using stress optic law. The magnitudes of isoclincs and isochromatics obtained from 8-step phase shifting method are compared with those of theories. From computer simulation, it is verified to separate isoclincs and isochomatics from photoelastic fringes.

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The Effects of Various Extracellular Matrices on Motility of Cultured MC3T3-E1 Cell (다양한 세포외기질이 배양 골아세포의 이동에 미치는 영향)

  • Park, Beyoung Yun;Seo, Sang Woo;Lee, Won Jai;Ryu, Chang Woo;Rah, Dong Kyun;Son, Hyun Joo;Park, Jong Chul
    • Archives of Plastic Surgery
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    • v.32 no.2
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    • pp.143-148
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    • 2005
  • Chemotactic migration of bone forming cell, osteoblast, is an important event during bone formation, bone remodeling, and fracture healing. Migration of cells is mediated by adhesion receptors, such as integrins, that link the cell to extracellular matrix ligands, type I collagen, fibronectin, laminin and depend on interaction between integrin and extracellular ligand. Our study was designed to investigate the effect of extracellular matrix like fibronectin, laminin, type I collagen on migration of osteoblast. Migration distance and speed of MC3T3-E1 cell on extracellular matrix-coated glass were measured for 24 hours using 0.01% type I collagen, 0.01% fibronectin, 100 microliter/ml laminin. The migration distance and speed of MC3T3-E1 cell was compared using a video-microscopy system. To determine migration speed, cells were viewed with a 4 phase- contrast lens and video recorded. Images were captured using a color CCD camera and saved in 8-bit full-color mode. The migration distance on 0.01% type I collagen or 0.01% fibronectin was longer than that on $100{\mu}l/ml$ laminin-coated glass. The migration speed on fibronectin-coated glass was 68 micrometer/hour which was fastest. The migration speed on type I collagen-coated glass was similar with that on fibronectin-coated glass. The latter two migration speeds were faster than that on no-coated glass. On the other hand, the average migration speed on laminin-coated glass was 37micrometer/hour and not different from that of control group. In conclusion, the extracelluar matrix ligands such as type I collagen and fibronectin seem to play an important role in cell migration. The type I collagen or fibronectin coated scaffold is more effective for migration of osteoblast in tissue engineering process.

Physicochemical and Archaeometric Characteristics of Goryeo Period Potteries from the Sandongri in Seosan, Korea (서산 산동리 고려시대 도기의 물리화학적 및 고고과학적 특성)

  • Lee, Chan Hee;Jin, Hong Ju
    • Journal of Conservation Science
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    • v.32 no.2
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    • pp.123-139
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    • 2016
  • The excavated potteries of Goryeo Period from the Sandongri archaeological site in Seosan were studied on physicochemical analyses. Surface color of the samples are mainly grayish blue, and showed the natural glaze by melting the body soils during the burning. Partly, swelling surface are observed bloated marks because of blow out gas by burning. The potteries are some possibility of making the similar source clay on the basis of magnetic susceptibilities (about $1{\times}10^{-3}SI\;unit$) and general occurrences. Values of specific gravity, apparent porosity and absorption ratio are divided two groups as highly different cases of bloating surface samples. The source clay of the potteries used mainly microcrystalline clay, the mineral compositions are quartz and some colored minerals. Based on the analysis, the burning temperature of the potteries are assumed that they were around $1,100^{\circ}C$ because detection of quartz and mullite within hard and compact matrices. As geochemical variations of the samples, evolution trends of rare earth, compatible and incompatible elements showed very similar patterns excepting the some major elements, that means the potteries are interpreted to making by elutriation processes using the same raw clays from very similar basement rocks of genetically.

Matrix effect on the Determination of Inorganic Priority Pollutants in Sludges (오니 시료중의 무기 Priority Pollutants의 분석 과정에 미치는 매질의 영향)

  • Lee, Huk-Hee;Lee, Sueg-Geun
    • Analytical Science and Technology
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    • v.11 no.4
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    • pp.297-304
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    • 1998
  • The three analysis methods, EPA method 3050, the method offered by Ministry of Environment in Korea, and modified method corrected in this laboratory, were studied to investigate the effect of matrix on the analysis of inorganic priority pollutants. 7 inorganic priority pollutants(Ni, Cr, Cu, Zn, Pb, Cd, Hg) were spiked to the plating, leather, paper, electric, and dye sludges. Mean recovery of the elements except Hg was 95.5% when the procedure of EPA method was applied. However, recovery by the two other extraction methods showed 11.1% and 27.7%, respectively. Digestions were done by MDS (microwave digestion system) and $HNO_3+HClO_4$ methods. To study organic and inorganic matrix effect, samples were made by adding triethanol amine as a organic matrix and $FeCl_3{\cdot}6H_2O$+$AlCl_3{\cdot}6H_2O$ as a inorganic matrix, respectively. The extracts were analyzed by AAS and HG-AAS. Mean recovery of the elements by the $HNO_3+HClO_4$ procedure, except Hg, gave better result than that of the MDS method. Mean recovery of elements was decreased when organic and inorganic matrices were added in the sludge samples. The procedure of MDS and $HNO_3+HClO_4$ digestion gave higher recoveries than that of direct analysis. In general, the results of the studies showed a significant matrix effect on the inorganic priority pollutants analysis in sludges.

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Development of Enhanced Analytical Method for Perchlorate in Food by IC-MS/MS with an Internal Standard (IC-MS/MS와 내부표준물질을 이용한 식품 중 perchlorate 분석법 개선)

  • Yang, Min-June;Kim, Jong-Sung;Han, Jong-Hun;Her, Nam-Guk
    • Korean Journal of Food Science and Technology
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    • v.43 no.5
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    • pp.532-536
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    • 2011
  • The internal standard method using ion chromatography combined with tandem mass spectrometry (IC-MS/MS) for quantifying perchlorate in different types of food was established. Because the presence of ions and other biomolecules in matrices usually interrupts accurate determination of perchlorate, it is necessary to develop efficient, reproducible method to determine perchlorate concentrations in food. In this study, the internal standard method was compared with the external standard method to determine perchlorate concentrations in standard samples. The obtained method detection limit (MDL) for perchlorate was 0.005 ${\mu}g/L$ for bottled water, 0.12 ${\mu}g/L$ for milk, 0.27 ${\mu}g/kg$ for rice, 0.16 ${\mu}g/kg$ for vegetables, and 0.07 ${\mu}g/kg$ for seaweed, respectively. The limit of quantitation (LOQ) was calculated by multiplying the MDL by 7. The recoveries of perchlorate from food samples spiked with perchlorate ranged from 98 to 105% and their percent relative standard deviation (%RSD) levels were <20%. This method was successfully applied for the quantitative determination of perchlorate in various kinds of food.

A Route-Splitting Approach to the Vehicle Routing Problem (차량경로문제의 경로분할모형에 관한 연구)

  • Kang, Sung-Min
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 2005.10a
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    • pp.57-78
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    • 2005
  • The vehicle routing problem (VRP) is to determine a set of feasible vehicle routes, one for each vehicle, such that each customer is visited exactly once and the total distance travelled by the vehicles is minimized. A feasible route is defined as a simple circuit including the depot such that the total demand of the customers in the route does not exceed the vehicle capacity. While there have been significant advances recently in exact solution methodology, the VRP is not a well solved problem. We find most approaches still relying on the branch and bound method. These approaches employ various methodologies to compute a lower bound on the optimal value. We introduce a new modelling approach, termed route-splitting, for the VRP that allows us to address problems whose size is beyond the current computational range of set-partitioning models. The route-splitting model splits each vehicle route into segments, and results in more tractable subproblems. Lifting much of the burden of solving combinatorially hard subproblems, the route-splitting approach puts more weight on the LP master problem, Recent breakthroughs in solving LP problems (Nemhauser, 1994) bode well for our approach. Lower bounds are computed on five symmetric VRPs with up to 199 customers, and eight asymmetric VRPs with up to 70 customers. while it is said that the exact methods developed for asymmetric instances have in general a poor performance when applied to symmetric ones (Toth and Vigo, 2002), the route splitting approach shows a competent performance of 93.5% on average in the symmetric VRPs. For the asymmetric ones, the approach comes up with lower bounds of 97.6% on average. The route-splitting model can deal with asymmetric cost matrices and non-identical vehicles. Given the ability of the route-splitting model to address a wider range of applications and its good performance on asymmetric instances, we find the model promising and valuable for further research.

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