• Korean journal of applied entomology
• /
• v.34 no.3
• /
• pp.169-173
• /
• 1995

This study was conducted to investigate control effects against Spodoptera exigua Hubner in field using mating disruption dispensers of synthetic sex pheromone during 1993 and 1994. Denisities in egg and larva of Spodoptera exigua in field treated with the dispensers was 1.9% level of that without dispensers in welsh onion, 12.5% in red pepper and 4.4% in potato in 1993. Also 5.9% and 5.2% were counted from 10 ha and 20 ha of welsh onion field compared. Control value in Fields with dispensers were maintained at 80% level in 10 ha and 90% level in 20 ha until October.

• Korean journal of applied entomology
• /
• v.47 no.1
• /
• pp.37-44
• /
• 2008

Oriental fruit moth, Grapholita molesta, is a serious pest on apples. To control this pest in an environmentally friendly method, mating disruption strategy using sex pheromone has been developed. Area-wide application of mating disruption has been needed to be effective, with little understanding on how much size of apple cultivating area should be treated in one time application of the mating disruption technique. On this matter, we needed to determine a minimal mating active zone of G. molesta that should be applied with mating disrupters to be effective. Molecular markers to discriminate a specific population should be developed to trace population migration for reproductive behaviors. Here we developed two effective molecular markers using random amplified polymorphic DNA (RAPD) technique. Different field populations of G. molesta, based on locations and seasons, were analyzed with these markers. In a specific location, G. molesta populations varied in genetic composition with different seasons. Different local populations showed differential variation according to their relative distances among apple orchards. In overall, genetic variation among different populations became lessen with progression of seasons.

• Korean journal of applied entomology
• /
• v.60 no.1
• /
• pp.15-47
• /
• 2021

Sex pheromone is used for chemical communication for mating in a species-specific manner in insects. Insect antennae possess sensory receptors specific to sex pheromone components and generate receptor potential to be perceived by the brain to evoke mating behavior. The sex pheromones have been used for monitoring specific species of insect pests to predict their subsequent occurrences based on a temperature-dependent growth model. Sex pheromones are also used for controlling pest insects using several different strategies such as mass capture, lure-and-kill, or mating disruption. This review explains the sensory physiology and insect pest management techniques related with sex pheromone.

• Korean journal of applied entomology
• /
• v.47 no.1
• /
• pp.51-57
• /
• 2008

This study demonstrates a variability in efficacy of mating disruption against the oriental fruit moth, Grapholita molesta, populations infesting apples cultivated in differential environmental conditions. Throughout the growing seasons, trap catches of G. molesta male moths and damaged leaf and fruit were examined to evaluate the efficacy of a commercialized mating disruptor. Treatment of the mating disruptor significantly reduced the trap catches of male moth in treated plot, compared to those of the untreated plot. This reduced trap catches were significantly correlated with leaf and fruit damage. Moreover, the highest host damage occurred in June just after the highest overwintering adult peaks in both plots. The treatment of mating disruptors in Chungsong effectively disrupted the overwintering population in April and May, resulting in no noticeable host damage during the following growing seasons. However, there was a marked difference in host damage between two plots, especially in late seasons. Variation in the efficacy of mating disruption technology in terms of host damage appeared to be related with nearby pheromone-untreated orchards, which may result in the immigration of gravid females.

• Korean journal of applied entomology
• /
• v.53 no.3
• /
• pp.209-215
• /
• 2014

This study was performed to monitor the seasonal occurrence of Pseudococcus comstocki (Kuwana) by using pheromone traps and to test a mating disruption technique to control this pest in pear orchards. We compared the attractiveness of rubber septa loaded with 1.5 and 3.0 mg of the pheromone, 2,6-dimethyl-1,5-heptadien-3-yl acetate. A total of 1,021 and 1,431 males were caught in traps baited with 1.5 mg and 3 mg of the pheromone, respectively. The numbers of males caught were not affected by trap color, although yellow traps were more attractive than white traps. In 2012, P. comstocki males were captured between June and October, with peaks in late June, early August, and late September. In 2013, the males were trapped between June and October, with peaks in middle June, late July, and late September. In pheromone mating disruption tests, catches were reduced by 17.7, 65.3, and 62.9% in orchards treated with 450, 900, and 1,350 mg per 10 a of the pheromone, respectively. At harvest, 3.4, 2.9, and 4.8% of fruits in orchards treated with 450, 900, and 1,350 mg per 10 a were damaged by P. comstocki, while 9.5% were damaged in the control orchard.

• Korean journal of applied entomology
• /
• v.45 no.2 s.143
• /
• pp.235-240
• /
• 2006

Efficacies of two commercial mating distruptor (SPLAT$^{(R)}$ and Isomate$^{(R)}$-ROSSO) were evaluated on field overwintering populations of Oriental fruit moth, Grapholita molesta (Busck), in four apple orchards. Based on the monitoring with sex pheromone traps, all the orchards (Youngchun, Kyungsan, Chungsong, and Youngju in Korea) exhibited significant overwintering populations from late April to late May. Both mating disruptors that were applied 10 days before the monitoring period significantly inhibited the male orientation to the monitoring lures, in which SPLAT type was more effective than Isomate type in the disrupting efficacy. The disruption of male orientation was highly correlated with the reduction in the early leaf damage caused mostly by G. molesta.

• Korean journal of applied entomology
• /
• v.46 no.2
• /
• pp.269-274
• /
• 2007

This study was performed to estimate the efficacy of three commercial mating disruptors for the control of oriental fruit moth, Grapholita molesta (Busck), using a food-baited trap to collect field females. Mated female ratios estimated in the mating disruptor-treated areas were compared with the estimates of male trap captures, and both estimates were evaluated on the basis of crop damage measured by shoot tip damage caused by G. molesta. Both males and females were attracted to the food trap-baited with terpinyl acetate in apple orchard. Spermatophore of G. molesta was similar to female bursa copulatrix in size. Though there was more than 95% reduction in male trap captures indicating significant mating disruption, significant crop damage occurred on apple leaf buds, in which more than 35% of captured females were mated. This study demonstrates that assessment of the mated females would be more reasonable to represent the efficacy of mating disruptor(s) than the assessment of the male captures in G. molesta.

• Korean journal of applied entomology
• /
• v.61 no.1
• /
• pp.63-75
• /
• 2022

Semiochemicals including pheromone are chemicals used in chemical communication of insect. Semiochemicals have been widely used for population monitoring, mass trapping, and mating disruption of insect pest. In this review article, the current status of pheromone research of major forest insect pest in Korea such as Monochamus alternauts, M. saltuarius, Matsucoccus thunbergianae, Platypus koryoensis, Glyphodes perspectalis, Dioryctria abietella, Lymantria dispar, Synanthedon bicingulata, and Naxa seriaria was introduced, and the results were compared with those reported in other countries. Based on the analysis of current pheromone research of forest insect pests, future studies and development direction was suggested.

• The Korean Journal of Pesticide Science
• /
• v.11 no.1
• /
• pp.46-51
• /
• 2007

In this study, two commercial mating disruptors were compared in terms of disruption of Oriental fruit moth, Grapholita molesta, male orientation, in which a new dispenser type, $SPLAT^{(R)}$, was compared with a current dispenser type, $Isomate^{(R)}$-M ROSSO. For this assessment, the last three year field monitoring data were analyzed for the efficacy of Isomate type dispenser. Then two commercial dispensers were compared in different localities with sub-locality replications during identical monitoring period from mid February to late August. There appeared to be four adult population peaks, in which the overwintering population size was positively correlated with the following reproductive population sizes. Isomate type dispenser effectively suppressed G. molesta populations during all growing seasons with some annual variation in its efficacy. Between two dispensers, SPLAT type was much effective. The difference in their efficacy may be caused by the difference in composition of major pheromone component.

• Korean journal of applied entomology
• /
• v.45 no.3 s.144
• /
• pp.309-316
• /
• 2006

Three sex pheromone components (cis-8-dodecenyl acetate (Z12Ac), trans-8-dodecenyl acetate (E12Ac), cis-8-dodecenol (Z12OH)) of the Oriental fruit moth, Grapholita molesta, were chemically synthesized. Especially to increase the composition of cis-stereoisomer, a triple bond intermediate was hydrogenated at $-20^{\circ}C$ with catalytic $Pd/BaSO_{4}$. The resulting product consisted of the acetates with a stereoisomer ratio in 92:8 (Z:E). The biological activity of the synthesized pheromone compounds was analyzed both in male responses and orientation disruption. The indoor pheromone effect was determined by male flight behavior showing wing movement in response to lure. Different mixtures of the synthetic pheromone components were prepared by mixing acetate and alcohol components in 100:0, 99:1, and 90:10 (g/g) and tested with a comparison of a standard commercial pheromone lure. The highest pheromone effect was observed in only acetate mixture (100:0) and the effect was reduced with the addition of the alcohol component. This indoor pheromone effect could be observed in field monitoring trial, in which 100:0 mixture showed the highest trap catches. Orientation disruption assay was conducted indoor by using a cage, in which the center had a commercial lure on sticky plate and the four candidates were placed at 6 cm away from the central lure on each of four directions. Test males were released to the arena during overnight (12 h) and then the caught males on the sticky plate were counted. The synthesized pheromone as well as the commercial pheromone showed 100% orientation disruption. However, the orientation disruption effect was reduced with decrease in the number of the surrounding disrupting pheromone baits. These results clearly suggest that the synthesized sex pheromone of G. molesta is biologically active and can be used for field mating disruption.