• Korean Journal of Pharmacognosy
• /
• v.49 no.1
• /
• pp.55-64
• /
• 2018

Recently, it has been a big issue to distinguish the dried roots of Cynanchum wilfordii and C. auriculatum in health functional food market. The original plant species of Cynanchi Wilfordii Radix belong to the Asclepiadaceae family is differentially described in the national pharmacopoeia of Korea, China and Japan. Owing to the morphological similarities of the dried roots of this plant to those of C. auriculatum, which is often misidentified in Korean herbal medicine marketplace, distinguishing these two species is exceedingly difficult. The purpose of this study was to compare the conventional-PCR with the real-time PCR for detection of C. wilfordii and C. auriculatum DNA. We also tried to realize a quantitative real-time PCR assay using species-specific matK primers, which allowed us to estimate the ratio of C. willfordii and C. auriculatum using varying ratios of mixed genomic DNA template from the two species. The differentiation of intentional and unintentional mixture in this study would be applied to food safety management and can be helpful for protection of consumer's right and cultivators.

• Research in Plant Disease
• /
• v.15 no.3
• /
• pp.259-261
• /
• 2009

Sclerotinia shoot rot of Grapevine(Vitis labruscana) occurred at Gapyeong and Yeongwol area from 2003 to 2005. Infected plants showed shoot blight at the one-year-old fruit bearing branches. The first visible symptom noticed was wilting and blighting of the branches. The obvious and typical initial symptom is the presence of a cottony, white, dense mat of mycelial growth on the surface of the diseased lesions. The base or stem of the infected young shoots develop a pale brown rotted area, which girdled and killed shoots. In advanced stages of the disease, stems and branches became bleached and eventually died. The isolates collected from diseased grapevine stem were identified as Sclerotinia sclerotiorum based on the morphological and cultural characteristics. The pathogenicity test revealed that Vitis vinifera(cultivar: Kyoho) was stronger than V. labruscana(cultivar: Campbell early) to the pathogen. This is the first report on sclerotinia shoot rot of grapevine in Korea.

• The Korean Journal of Nuclear Medicine Technology
• /
• v.15 no.1
• /
• pp.106-112
• /
• 2011

Purpose: Until now, the process was improved by the needs of experimenters personally. But recently, suggestion system in hospital has been activated in various ways. So the department of nuclear medicine laboratory is also aware of the need of operation improvement using suggestion system. It is intend to assist in the development by sharing excellent suggestion cases with other hospitals. Material & Method: A total of 124 suggestion cases from January 2007 to March 2010 were analyzed. Suggestion cases were divided into customer satisfaction, cost reduction, improved testing methods, equipment, environmental improvement, and computational system. Result: Suggestion cases of environmental improvement and computational system were accounted for 26.6% as 33 cases, respectively. Suggestion for customer satisfaction is 25.8% as 32 in a total of 124 cases. Conclusion: Activation of the awareness of operation improvement is induced by suggestion system. By securing system of operation improvement, employees' ideas can lead to the production and systematization. Furthermore, it enhances hospital competitiveness and promotes the development of the hospital.

• Journal of Internet Computing and Services
• /
• v.8 no.1
• /
• pp.33-45
• /
• 2007

Using switches and sensors informing the current on or off state, this paper suggests a sleeping control and remote monitoring system that not only can recognize the sleeping situations but also can control for keeping an appropriate sleeping situation remotely, And we show an example that this system is applied to the healthcare sleeping mat, Our system comprises the following 3 parts: a part for detecting the sleeping situations, a part for extracting sensing data and sending/receiving the relating situated data, and a part controlling and monitoring the all of sleeping situations. In details, in order to develop our system, we used the touch and pressure-sensitive sensors with On/Off functions for a purpose of the first part, The second part consists of the self-developed embedded board with the socket based communication as well as extracting real-time sensing data. And the third part is implemented by service modules for providing controlling and monitoring functions previously described. Finally, these service modules are implemented by the TMO scheme, one of real-time object-oriented programming models and the communications among them is supported using the TMOSM of distributed real-time middleware.

• The Journal of the Korean Society for Microbiology
• /
• v.21 no.2
• /
• pp.181-189
• /
• 1986

To apply ELISA to serodiagnosis of leptospirosis with killed whole cells from Leptospira interrogans serovars mwogolo (Mwogolo), copenhageni (M-20), WH-20, autumnalis (Akiyami A), cynopteri (3522 C), australis (Bacillico) and Leptospira biflexa serovar patoc (patoc 1), sensitivity and specificity was evaluated. The reactivity of IgM and IgG antibody in the sera from patients with leptospirosis, hemorrhagic fever with renal syndrome and other febrile disease and normal healthy control to the killed whole cells was analysed. The results were summarized as follows. 1. The reactivity (absorbance at 492mn) of IgM and IgG to L. mwogolo antigen in the sera of pattients with leptospirosis were $1.414{\pm}0.370$, $1.242{\pm}9.554$ respectively: hemorrhagic fever with renal syndrome, $0.329{\pm}0.131$, $0.239{\pm}0.126$; other febrile disease, $0.196{\pm}0.071$, $0355{\pm}0.141$; normal healthy control, $0.136{\pm}0.016$, $0.208{\pm}0.077$. 2. The reactivity of IgM and IgG to L. copenhageni, WH-20, L. autumnalis, L. cynopteri and L. anstralis antigens were similar to that to L. mwogolo antigen, but that to L. biflexa antigen was not discriminated among above disease. 3. Correlation coefficient between the MAT titer and ELISA OD (IgM) to the above antigens was in the range of 0.071-0.518. 4. As absorbance above 0.60 was determined positive for the diagnosis of leptospirosis, the sensitivity and specificity of IgG was 25-89% and 91-96% respectively. And those of IgM was 98-100% and 89-100% except L. biflexa (29%) respectively.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.27 no.3
• /
• pp.393-400
• /
• 2009

Recently demand for the 3D modeling technology to reconstruct real world is getting increasing. However, existing geospatial data are mainly based on the 2D space. In addition, most of the geospatial data provide geometric information only. In consequence, there are limits in various applications to utilize information from those data and to reconstruct the real world in 3D space. Therefore, it is required to develop efficient 3D mapping methodology and data for- mat to establish geospatial database. Especially digital elevation model(DEM) is one of the essential geospatial data, however, DEM provides only spatially distributed 3D coordinates of the natural and artificial surfaces. Moreover, most of DEMs are generated without considering terrain properties such as surface roughness, terrain type, spatial resolution, feature and so on. This paper suggests adaptive and flexible geospatial data format that has possibility to include various information such as terrain characteristics, multiple resolutions, interpolation methods, break line information, model keypoints, and other physical property. The study area was categorized into mountainous area, gently rolling area, and flat area by taking the terrain characteristics into account with respect to terrain roughness. Different resolutions and interpolation methods were applied to each area. Finally, a 3D digital map derived from aerial photographs was integrated with the geospatial data and visualized.

• Applied Chemistry for Engineering
• /
• v.10 no.2
• /
• pp.252-258
• /
• 1999

Tetramethyl orthosilicate (TMOS) was obtained by the direct synthesis of methanol with metallic silicon including copper compound as a catalyst and zinc compound as a promoter. The effects of the preheating temperature and the preparation method of the contact mass on TMOS synthesis were investigated. The composition effects of the contact mass which was composed of metallic silicon with copper catalyst and various metallic halide promoters including Zn, Sn or Cd compound were studied also. The best performance on TMOS synthesis was observed on a mixed bed reactor containing metallic silicon preheated with CuCl as a catalyst and $ZnCl_2$ as a promoter. When Cu/Si = 7 wt %, Zn/Cu = 7 wt % was mixed in a slurry phase and activated into contact mass at $380^{\circ}C$, the average selectivity was 87.2% in the silicon consumption of 69.2% at $220^{\circ}C$.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.15 no.2
• /
• pp.161-169
• /
• 2011

Two-way slabs reinforced with high-performance steels, which have several practical advantages of a reduction of congestion in heavily reinforced members, savings in the cost of labor and repair, the higher corrosion resistance, and a reduction of construction time, were constructed and tested. The influences of the flexural reinforcement ratio, concentrating the reinforcement in the immediate column region, and using steel fiber-reinforced concrete (SFRC) in the slab on the punching shear resistance and post-cracking stiffness were investigated, and compared with the punching shear test results of the slabs reinforced with conventional steels and GFRP bars. In addition, the strain distribution of flexural reinforcements and crack control were investigated, and the effective width calculating method for the average flexural reinforcement ratio was estimated. The use of high-performance steel reinforcement increased the punching shear strength of slabs, and decreased the amount of flexural reinforcements. The concentrating the top mat of flexural reinforcement increased the post-cracking stiffness, and showed better strain distribution and crack control. In addition, the use of SFRC showed beneficial effects on the punching shear strength and crack control. It was suggest that the effective width should be changed to larger than 2 times the slab thickness from the column faces.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.37 no.2
• /
• pp.198-208
• /
• 1992

Recently, a nursery mat made from rock wool has realized transplanting of the younger seedlings with the ordinary transplanting machines for Chibyo and Chubyo(3 and 4～5 leaved seedling, respectively). The seedlings defined as the 'Nyubyo' or 'Nursling seedlings' became possible to achieve economic profits from the reduction in both working time and costs. It being widely noticed as a strategy to solve the difficulties in current rice cultivation. The nursling seedlings are 1.4 to 2.5 leaves and height at 4.5 to 7cm, grown 4 to 7 days after seeding. They maintain still up to 50 to 80% of their nutrients in the endosperm, and can grow by using only their own nutrients for a certain period of time after transplanting. Nursling seedlings take 2 days in the nursery chamber at 32$^{\circ}C$ after seeding, and 2 days in the greening house at $25^{\circ}C$. This is only 4 days, all together, to make the nursling seedlings of 1.5 leaves which are ready for transplanting. Watering is only needed once at the sowing time. It only takes 1 or 2 waterings even to raise a seedlings for a period of 7 days. The number of nursery boxes can be reduced because it is possible to sow more densely(220 to 240g per box), thus it only needs seedlings of 15 to 16 boxes per 10 a which leads to a reduction in facilities and space needed. Temperature during the nursery period can be artificially adjusted more precisely which may lead to the prevention of temperature stress. The nursling seedlings can root rapid by because the crown roots from the coleoptile node begin to emerge immediately after transplanting. They show strong resistance to low temperature (12$^{\circ}C$) and deep-planting. There is no danger in the rooting of the seedlings even if half of their height is buried into the soil. Moreover, it can root at a rate of up to 65 to 80% even if the full height of the seedlings is buried. They show also strong resistance to submergence (10～15cm). The nursling seedlings tend to grow by producing tillers from lower nodes. It is therefore, necessary to control to keep the proper numbers of tillers per unit area. They have no fear in the delay of heading and their yield components can be so well balanced that the same level of yield was achieved with the nursling seedlings compared to that with Chibyo. It was further suggested that if the surplus tillers can be avoided by such cultivation practices, the number of grain per panicle can be kept greater and higher yield can be realized. Practical experiments with the nursling seedlings conducted in 1989 and 1990 by farmers in various areas showed exciting results. The nursling seedlings will become widely spread, or at least occupy an important position in Japanese and also in Korean rice cultivation techniques.tivation techniques.

• Journal of Life Science
• /
• v.20 no.11
• /
• pp.1738-1741
• /
• 2010

Caliper measurements of tumor volume have been widely used in the assessment of tumors in animal models. However, experiments based on caliper data have resulted in unreliable estimates of tumor growth, due to necrotic areas of tumor mass. To overcome this systematic bias, we engineered a new luciferase-expressing rat prostate cancer cell line (MLL-Luc) that produces bioluminescence from viable cancer cells. MLL-Luc cells showed a strong correlation between bioluminescence intensity and cell number ($R^2$=0.99) and also accurately quantified tumor growth, with reduced bioluminescence signals caused by necrotic cells in a subcutaneous MLL-Luc xenograft model. The accurate quantification of tumor growth with bioluminescence imaging (BLI) was confirmed by a better antitumor effect of combination chemotherapy, compared to that based on caliper measurements with a correlation between the bioluminescence signal and tumor volume ($R^2$=0.84). These data suggest that bioluminescent MLL xenografts are a powerful and quantitative tool for monitoring tumor growth and are useful in evaluating the efficacy of anticancer drugs, with less systematic bias.