• Analytical Science and Technology
• /
• v.20 no.5
• /
• pp.424-433
• /
• 2007

An analytical method for the determination of thyroid hormones in plasma samples has been studied by solid-phase extraction and high-performance liquid chromatography/diode array detector (DAD)/electrospray ionization (ESI)-mass spectrometer. Seven thyroid hormones were successfully separated by gradient elution on the reverse phase Hypersil ODS column (4.6 mm I.D., 250 mm length, particle size $5{\mu}m$) with ammonium formate buffer and acetonitrile. In addition, these compounds were confirmed by UV spectra and ESI-mass Spectra. The extraction recoveries of thyroid hormones in the plasma sample (at pH 3) were in the range of 74.5-115.7 % with solid-phase extraction by C18, followed by elution with 4 mL of methanol. The calibration curves showed good linearity with the correlation coefficients ($r^2$) varying from 0.9939 to 0.9978 and the detection limits of all analytes were obtained in the range of 20-50 ng/mL (38.1-162.8 pmol/mL). As a result, thyroxine was found in the range of 50.98-112.97 ng/mL in normal plasma samples.

• Proceedings of the KSME Conference
• /
• 2003.04a
• /
• pp.834-838
• /
• 2003

Vibration of turbine is concerned with array of last moving blade at lower pressure turbine. When last moving blade at lower pressure turbine was replaced, we must consider mass unbalance problems of blades. If mass unbalance happened at rotor, it is impossible to operate turbine. In this paper, we have how to minimize the mass unbalance problems of last moving blade at lower pressure turbine.

• Journal of The Korean Astronomical Society
• /
• v.27 no.2
• /
• pp.159-175
• /
• 1994

We have mapped the whole extent of a dark cloud Lynds 1251 in the emission of the J=1-0 transitions of $^{12}CO\;and\;^{13}CO$ using FCRAO's fifteen-beam array receiver in high angular resolution of 50'. We have derived physical parameters of L1251, discussed three different mass estimate techniques, and obtained a large range of mass, 600 to $6,000M_\bigodot$, depending on the techniques. The factor of 10 discrepancy between the virial and LTE masses is much larger than expected based on the uncertainties residing in two methods. The large virial mass may reflect the fact that L1251 is not gravitationally bound system as in the case of dark clouds in solar neighborhood. Two outflows are affecting the dynamics of cloud significantly but not enough to reshape the whole extent of the cloud. The small cloud, 'Stripe', which is apparently connected with main cloud, is not likely to be associated with L1251. The velocity gradient composed on this small cloud may be driven by other unknown sources. It is found that L1251 cloud itself is very quiescent except the two bipolar outflow regions.

• Mass Spectrometry Letters
• /
• v.4 no.1
• /
• pp.1-9
• /
• 2013

Fungal biotechnology has been well established in food and healthcare sector, and now being explored for lignocellulosic biorefinery due to their great potential to produce a wide array of extracellular enzymes for nutrient recycling. Due to global warming, environmental pollution, green house gases emission and depleting fossil fuel, fungal enzymes for lignocellulosic biomass refinery become a major focus for utilizing renewal bioresources. Proteomic technologies tender better biological understanding and exposition of cellular mechanism of cell or microbes under particular physiological condition and are very useful in characterizing fungal secretome. Hence, in addition to traditional colorimetric enzyme assay, mass-spectrometry-based quantification methods for profiling lignocellulolytic enzymes have gained increasing popularity over the past five years. Majority of these methods include two dimensional gel electrophoresis coupled to mass spectrometry, differential stable isotope labeling and label free quantitation. Therefore, in this review, we reviewed more commonly used different proteomic techniques for profiling fungal secretome with a major focus on two dimensional gel electrophoresis, liquid chromatography-based quantitative mass spectrometry for global protein identification and quantification. We also discussed weaknesses and strengths of these methodologies for comprehensive identification and quantification of extracellular proteome.

• Proceedings of the KSME Conference
• /
• 2003.04a
• /
• pp.2219-2226
• /
• 2003

Two perforated plates are placed in parallel and staggered arrangements with a gap distance of 2 times of the hole diameter, and initial crossflow passes between the plates. Both the injection and effusion hole diameters are 10 mm, and the Reynolds number based on the hole diameter and hole-to-hole pitch are fixed to 10,000 and 6 times of the hole diameter, respectively. To investigate the effect of crossflow, the flow rate of crossflow is changed from 0.2 to 2 times of that of the impinging jet. A naphthalene sublimation method is used to determine the local heat/mass transfer coefficients on the upward facing surface of the effusion plate. With the initial crossflow, the heat/mass transfer rates on the effusion (target) plate decrease as the velocity of crossflow increases, since the crossflow induces the locally low transfer regions formed at the mid-way between the effusion holes. However, the impingement/effusion cooling with crossflow presents higher heat/mass transfer rates than the array jet impingement cooling with the same initial crossflow.

• Nuclear Engineering and Technology
• /
• v.55 no.1
• /
• pp.109-118
• /
• 2023

In this paper, the evaluation method of fluidelastic instability (FEI) of newly designed steam generator tubing in pressurized water reactor (PWR) nuclear power plants is discussed. To obtain the parameters for prediction of the critical velocity of FEI for steam generator tubes, experimental research is carried out, and the design parameters are determined. Using CFD numerical simulation, the tube array scale of the model experiment is determined, and the experimental device is designed. In this paper, 7 groups of experiments with void fractions of 0% (water), 10%, 20%, 50%, 75%, 85% and 95% were carried out. The critical damping ration, fundamental frequency and critical velocity of FEI of tubes in flowing water were measured. Through calculation, the total mass and instability constant of the immersed tube are obtained. The critical damping ration measured in the experiment mainly included two-phase damping and viscous damping, which changed with the change in void fraction from 1.56% to 4.34%. This value can be used in the steam generator design described in this paper and is conservative. By introducing the multiplier of frequency and square root of total mass per unit length, it is found that the difference between the experimental results and the calculated results is less than 1%, which proves the rationality and feasibility of the calculation method of frequency and total mass per unit length in engineering design. Through calculation, the instability constant is greater than 4 when the void fraction is less than 75%, less than 4 when the void fraction exceeds 75% and only 3.04 when the void fraction is 95%.

• Transactions of Materials Processing
• /
• v.18 no.4
• /
• pp.310-316
• /
• 2009

Micro lens array(MLA) is widely used in information technology(IT) industry fields for various applications such as a projection display, an optical power regulator, a micro mass spectrometer and for medical appliances. Recently, MLA have been fabricated and developed by using a reflow method having the processes of micro etching, electroplating, micro machining and laser local heating. Laser thermal relaxation method is introduced in marking of microdots on the surface of densified glass. In this paper, we have proposed a new direct fabrication process using UV laser local thermal-expansion(UV-LLTE) and investigated the optimal processing conditions of MLA on the surface of negative photo-resist material. We have also studied the 3D shape of the micro lens obtained by UV laser irradiation and the optimal process conditions. And then, we made chrome mold by electroplating. After that, we made MLA using chrome mold by hot embossing processing. Finally, we have measured the opto-physical properties of micro lens and then have also tested the possibility of MLA applications.

• Laser Solutions
• /
• v.11 no.2
• /
• pp.26-32
• /
• 2008

Micro lens array (MLA) is widely used in information technology (IT) industry fields, for examples such as a projection display, an optical power regulator, a micro mass spectrometer and for medical appliances. Recently, MLA have been fabricated and developed by using a reflow method, micro etching, electroplating, micromachining and laser local heating. Laser local thermal-expansion (LLTE) technology demonstrates the formation of microdots on the surface of polymer substrate, in this paper. We have also investigated the new direct fabrication method of placing the MLA on the surface of a SU-8 photoresist layer. We have obtained the 3D shape of the micro lens processed by UV laser irradiation and have experimentally verified the optimal process conditions.

• Smart Structures and Systems
• /
• v.25 no.4
• /
• pp.393-399
• /
• 2020

In this paper, the design method and experimental validation for the two-degree-of-freedom (2DOF) electromagnetic energy harvester are presented. The harvester consists of the rigid body suspended by four tension springs and electromagnetic transducers. Once the two resonant frequencies and the mass properties are specified, both the constant and the positions for the springs can be determined in the closed form. The designed harvester can locate two resonant peaks close to each other and forms the extended frequency bandwidth for power harvesting. Halbach magnet array is also introduced to enhance the output power. In the experiment, two resonant frequencies are measured at 34.9 and 37.6 Hz and the frequency bandwidth improves to 5 Hz at the voltage level of 207.9 mV. The normalized peak power of 4.587 mW/G² is obtained at the optimal load resistor of 367 Ω.

• Proceedings of the Korean Society for Technology of Plasticity Conference
• /
• 2002.05a
• /
• pp.94-97
• /
• 2002

Micromolding methods are most suitable for mass production of plastic microlens and lens array with low cost. Among the procedures related with micromolding of microlens array, fabrication of mold insect which contains micro cavity of lens shape is the most important stage. In this study, nickel mold inserts for 45 $\mu\textrm{m}$ and 95 $\mu\textrm{m}$ diameters lens way were fabricated using electroforming process. The mother for metal mold inset was made using reflow method. A micro compression molding with polymer powders was used to test the qualities of the metal mold insets. Micro lens profile and surface roughness was measured by interferometric technique and AFM, respectively. The final molded lens replicated the mother well, and had good surface quality.