• Journal of Microbiology and Biotechnology
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• v.21 no.5
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• pp.483-493
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• 2011

An acid phosphatase (HppA) activated by $NH_4Cl$ was purified 192- and 34-fold from the periplasmic and membrane fractions of Helicobacter pylori, respectively. SDS-polyacrylamide gel electrophoresis revealed that HppA from the latter appears to be several kilodaltons larger in molecular mass than from the former by about 24 kDa. Under acidic conditions (pH${\leq}$4.5), the enzyme activity was entirely dependent on the presence of certain mono- and/or divalent metal cations (e.g., $K^+$,$ NH_4{^+}$, and/or $Ni^{2+}$). In particular, $Ni^{2+}$ appeared to lower the enzyme's $K_m$ for the substrates, without changing $V_{max}$. The purified enzyme showed differential specificity against nucleotide substrates with pH; for example, the enzyme hydrolyzed adenosine nucleotides more rapidly at pH 5.5 than at pH 6.0, and vice versa for CTP or TTP. Analyses of the enzyme's N-terminal sequence and of an $HppA^-$ H. pylori mutant revealed that the purified enzyme is identical to rHppA, a cloned H. pylori class C acid phosphatase, and shown to be the sole bacterial 5'-nucleotidase uniquely activated by $NH_4Cl$. In contrast to wild type, $HppA^-$ H. pylori cells grew more slowly. Strikingly, they imported $Mg^{2+}$ at a markedly lowered rate, but assimilated urea rapidly, with a subsequent increase in extracellular pH. Moreover, mutant cells were much more sensitive to extracellular potassium ions, as well as to metronidazole, omeprazole, or thiophenol, with considerably lowered MIC values, than wild-type cells. From these data, we suggest that the role of the acid phosphatase HppA in H. pylori may extend beyond 5'-nucleotidase function to include cation-flux as well as pH regulation on the cell envelope.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2006.11a
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• pp.34-40
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• 2006

For a cooling performance research of the combustor operated in a extreme environment of a high temperature and high pressure, we accomplished a cooling performance analysis. Generally a heat transfer characteristic in cooling passage is known well experimentally and theoretically, however heat flux in the combustion chamber isn't. In this study, fluid flow combined with heat transfer and thermal structural analysis is accomplished about a combustor nozzle. We tried to analyze the cooling performance with a heat transfer characteristic of a gas and coolant side in the view point of quantity on the mass flow rate to be supplied to the cooling channel. And finally, evaluation on the thermal and structural safety of nozzle wall material was accomplished.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.2
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• pp.57.2-57.2
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• 2014

Red active galactic nuclei (AGNs) are supposed to be transitional objects becoming normal AGNs in the galaxy evolution scenario. So far, ~200 red AGNs have been found by very red color in optical through NIR wavelength (e.g., r'-K >5 and J-K>1.3; Urrutia et al. 2009). Here, we compare nuclear activities of the red AGNs to those of normal AGNs to verify the evolutionary phase of the red AGNs. In order to study the nuclear activities of the red AGNs, we use broad emission lines of $P{\beta}$ ($1.28{\mu}m$) of which flux is less suppressed by a factor of 100 than the $H{\beta}$ line in the case of the red AGNs with a color excess of E(B-V)=2 mag. We use 16 red AGNs discovered in previous red AGN surveys by using SDSS, 2MASS, and FIRST (Glikman et al. 2007; Urrutia et al. 2009) at z ~0.7 for which $P{\beta}$ lines are redshifted to the sky window at ${\sim}2.2{\mu}m$. The mean Eddington ratio of the 16 red AGNs is 0.562, and that of the normal AGNs is 0.320, which indicates the red AGNs include more active black hole (BH) than the normal AGNs. To test how significantly the nuclear activities of the red AGNs and the normal AGNs are different, we perform a two-dimensional Kolmogorov-Smirnov test (K-S test) on their Eddington ratio distributions. The K-S test shows the maximum deviation between the cumulative distributions, D, is 0.48, and the probability of null hypothesis, p, is even less than 0.001. This result is consistent with a picture of that the red AGNs are in intermediate phase between the stage of merger-driven starburst galaxy and the normal AGN.

• Proceedings of the KIEE Conference
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• 2001.07c
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• pp.1921-1923
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• 2001

In this work, an array of resistance temperature detector(RTD) was fabricated inside the microchannel in order to investigate in-situ flow characteristics. A rectangular straight microchannel, integrated with RTD's for temperature sensing and a heat source for generating the temperature gradient along the channel. were fabricated with the dimension of $200{\mu}m(W){\times}{\mu}m(D){\times}$48mm(L), while RTD measured precise temperatures at the inside-channel wall. 4" $525{\pm}25{\mu}m$ thick P-type <100> Si wafer was used as a substrate. For the fabrication of RTDs. 5300$\AA$ thick Pt/Ti layer was sputtered on a Pyrex glass wafer. Finally, glass wafer was bonded with Si wafer by anodic bonding, therefore RTD was located inside the microchannel. The temperature distribution inside the fabricated microchannel was obtained from 4 point probe measurements and Dl water is used as a working fluid. Temperature distribution inside the microchannel was measured as a function of mass flow rate and heat flux. As a result, precise temperatures inside the microchannel could be obtained. In conclusion, this novel temperature distribution measurement system will be very useful to the accurate analysis of the flow characteristics in the microchannel.

• Journal of Environmental Science International
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• v.18 no.11
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• pp.1271-1281
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• 2009

The heavy snowfall event over the eastern part of Seoul, Korea on Mar. 04, 2008 has been abruptly occurred after the frontal system with the heavy snowfall event had been past over the Korean peninsula on Mar. 03, 2008. Therefore, this heavy snowfall event couldn't be predicted well by any means of theoretical knowledges and models. After the cold front passed by, the cold air mass was flown over the peninsula immediately and became clear expectedly except the eastern part and southwestern part of peninsula with some large amount of snowfall. Even though the wide and intense massive cold anticyclone was expanded and enhanced by the lowest tropospheric baroclinicity over the Yellow Sea, but the intrusion and eastward movement of cold air to Seoul was too slow than normally predicted. Using the data of numerical model, satellite and radar images, three dimensional analysis Products(KLAPS : Korea Local Analysis and Prediction System) of the environmental conditions of this event such as temperature, equivalent potential temperature, wind, vertical circulation, divergence, moisture flux divergence and relative vorticity could be analyzed precisely. Through the analysis of this event, the formation and westward advection of lower cyclonic circulation with continuously horizontal movement of air into the eastern part of Seoul by the analyses of KLAPS fields have been affected by occurring the heavy snowfall event. As the predictability of abrupt snowfall event was very hard and dependent on not only the synoptic atmospheric circulation but also for mesoscale atmospheric circulation, the forecaster can be predicted well this event which may be occurred and developed within the very short time period using sequential satellite images and KLAPS products.

• Journal of Advanced Marine Engineering and Technology
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• v.38 no.9
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• pp.1032-1038
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• 2014

In this study, an experimental study was performed to understand the boiling heat transfer characteristics of FC-72 in parallel micro-channels. The parallel micro-channels contained channels having a $0.2mm{\times}0.45mm$ [$H{\times}W$] cross section and length of 60 mm. And heat flux was varied from 16.4 to $25.6kW/m^2$ and mass fluxes from 300 to $500kg/m^2s$. The measured heat transfer coefficient was sharply decreased at lower vapor quality and then it was kept approximately constant as the vapor quality is increased. From the experimental results, the boiling heat transfer mechanism of FC-72 was confirmed and the measured heat transfer coefficient was compared and analyzed with the existing correlations to predict the heat transfer coefficient.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.36 no.2
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• pp.225-232
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• 2012

A chemical reactor network (CRN) was developed for a lean premixed gas turbine combustor to predict the emission of pollutants such as NOx and CO. In this study, the predictions of NOx and CO emissions from lean premixed methane-air combustion in the gas turbine were carried out using CHEMKIN and a GRI 3.0 methane-air combustion mechanism, which includes the four NO formation mechanisms for various load conditions. The calculated results were compared with experimental data obtained from a modified test combustor to validate the model. The contributions of the four NO pathways were investigated for various load conditions. The effects of nonuniformity of the mass flux and of the equivalence ratio of the injector on the NOx formation were investigated, and a method of reducing the pollutant formation was suggested for the development of a sub-10 ppm gas turbine combustor.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.29 no.5 s.236
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• pp.643-652
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• 2005

Saturated nucleate pool boiling experiments for binary mixtures, which are consisted of refrigerant R11 and R113, were performed with constant wall temperature condition. Results for binary mixtures were also compared with pure fluids. A microscale heater array and Wheatstone bridge circuits were used to maintain the constant temperature of the heating surface and to obtain heat flow rate measurements with high temporal and spatial resolutions. Bubble growth images were captured using a high speed CCD camera synchronized with the heat flow rate measurements. The departure time for binary mixtures was longer than that for pure fluids, and binary mixtures had a higher onset of nucleate boiling (ONB) temperature than pure fluids. In the asymptotic growth region, the bubble growth rate was proportional to a value between $t^{\frac{1}{6}}$ and $t^{\frac{1}{4}}$. The bubble growth behavior was analyzed to permit comparisons with binary mixtures and pure fluids at the same scale using dimensionless parameters. There was no discernable difference in the bubble growth behavior between binary mixtures and pure fluids for a given ONB temperature. And the departure radius and time were well predicted within a ${\pm}30{\%}$ error. The minimum heat transfer coefficient of binary mixtures occurred near the maximum ${\mid}y-x{\mid}$ value, and the average required heat flux during bubble growth did not depend on the mass fraction of R11 as more volatile component in binary mixtures. Finally, the results showed that for binary mixtures, a higher ONB temperature had the greatest effect on reducing the heat transfer coefficient.

• The Korean Journal of Nuclear Medicine
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• v.38 no.3
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• pp.268-271
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• 2004

A 43-year-old man was presented with persistent headache for two weeks. 72 weighted MR imaging showed high signal intensity with surrounding edema in the left frontal lobe. These findings were considered with intracranial tumor such as glioma or metastasis. Tc-99m tetrofosmin SPECT showed focal radiotracer accumulation in the left frontal lobe. The operative specimen contained cerebral infarction with organizing leptomeningeal hematoma by pathologist. Another 73-year-old man was hospitalized for chronic headache. Initial CT showed ill-defined hypodensity with mass effect in the right parietal lobe. Tc-99m tetrofosmin SPECT showed focal radiotracer uptake in the right parietal lobe. These findings were considered with low-grade glioma or infarction. Follow-up CT after 5 months showed slightly decreased in size of low density in the right parietal lobe, and cerebral infarction is more likely than others. Tc-99m tetrofosmin has been proposed as a cardiotracer of myocardial perfusion imaging and an oncotropic radiotracer. Tc-99m tetrofosmin SPECT image provides a better attractive alternative agent than T1-201 as a tumor-imaging agent, with characteristics such as high-energy flux, short half-life, favorable biodistribution, dosimetry and lower background radioactivity. We have keep in mind on the analysis of Tc-99m tetrofomin imaging when cerebral infarction is being differentiated from brain tumor.

• Nuclear Engineering and Technology
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• v.32 no.4
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• pp.395-409
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• 2000

Parametric studies were performed to assess the sensitivity in determining the maximum in-vessel heat removal capability from the core material relocated into the lower plenum of the reactor pressure vessel (RPV）during a core melt accident. A fraction of the sensible heat can be removed during the molten jet delivery from the core to the lower plenum, while the remaining sensible heat and the decay heat can be transported by rather complex mechanisms of the counter-current flow limitation (CCFL) and the critical heat flux (CHF）through the irregular, hemispherical gap that may be formed between the freezing oxidic debris and the overheated metallic RPV wall. It is shown that under the pressurized condition of 10MPa with the sensible heat loss being 50% for the reactors considered in this study, i.e. TMI-2, KORI-2 like, YGN-3&4 like and KNGR like reactors, the heat removal through the gap cooling mechanism was capable of ensuring the RPV integrity as much as 30% to 40% of the total core mass was relocated to the lower plenum. The sensitivity analysis indicated that the cooling rate of debris coupled with the sensible heat loss was a significant factor The newly proposed heat removal capability map (HRCM) clearly displays the critical factors in estimating the maximum heat removal from the debris in the lower plenum. This map can be used as a first-principle engineering tool to assess the RPV thermal integrity during a core melt accident. The predictive model also provided ith a reasonable explanation for the non-failure of the test vessel in the LAVA experiments performed at the Korea Atomic Energy Research Institute (KAERI), which apparently indicated a cooling effect of water ingression through the debris-to-vessel gap and the intra-debris pores and crevices.